Criopreservação de embriões bovino produzidos in vitro: estado da arte

In the last years, the embryo in vitro production for every domestic species and mainly for bovine has attained a notorius status. This reproductive biotechnical procedure associate with ultrasound-guided ovum pick up (OPU) has been more and more incorporated and spread in our cattle herds, ranking up Brazil already at the top of the list in number of in vitro embryo produced. Some significant advantages provided, such as the possibility of using the premature or pregnant animals oocytes, without necessarily requiring the use of hormonal treatment, to make it possible to generate pregnancy at a shorter period of time, the rationalization in the use of semen and optimization in the use of sexed semen were determinant factors for OPU/IVP to reach this outstanding position. Nevertheless, right now the possibility of IVP embryo cryopreservation, just now is the biggest impediment for maximizing the use of this biotechnology, due to both lack of efficient methods and low laboratory produced embryo cryotolerance. Nowadays, the most used methods of IVP embryo cryopreservation are: slow freezing and vitrification. Traditionally, slow freezing is still the most used methods for in vivo and in vitro produced embryo cryopreservation. However, more recently vitrification - although still not commercially used in large scale - has been presenting satisfactory results in IVP embryo cryopreservation, according to searches

Remoção de N-amoniacal em baixas concentrações de oxigênio dissolvido a partir do efluente de frigorífico bovino

This work aimed to develop a biological system for removal of ammonia nitrogen operating at low concentrations of dissolved oxygen. Thus, a biological upflow vertical reactor was built, in which the affluent pass through the support media until the top. Sludge from an anaerobic stabilization pond of a slaughterhouse unit in the city of Presidente Prudente - SP was used as inoculum. Initially the system operated in batch and afterwards in a continuous flow with different HRT. For feeding the reactor, an initial phases was adopted a synthetic culture media, described by Martins (2007), in order to establish the ideal conditions for the development of Anammox bacteria and subsequently, submitted to the system a slugde effluent of slaughterhouse. The results showed significant removal efficiency of N-NH4+, especially in the phase without recirculation of culture media, with an average of 71% removal, with the proportion of removal of N-NH4 +:N-NO2- average 1: 1,69. For the period of operation with effluent from the slaughterhouse, were not obtained satisfactory results, without confirmation of the proliferation of Anammox bacteria in the system, due to the high presences of organic matter in the same confirmed by high concentrations of COD

Tratamento de efluentes provenientes de abatedouro de bovinos por meio de reator enzimático seguido de reator aeróbio operado em bateladas sequenciais

Brazil has one of the largest cattle herds in the world, so the cattle slaughter is one of the most important economic activities in the Brazilian market. But this activity requires a high demand of water, resulting in serious problems about the correct disposal of wastewater generated in the process. This effluent has a high pollution load, becoming its receiving bodies (streams and rivers) unfit for various activities such as public water supply, recreation, fisheries. To minimize the environmental impacts of its industrial wastewater and fallow the local environmental legislation, refrigerators must make the treatment of these effluents. This study aimed to verify the efficiency of a enzymatic reactor, when occur hydrolysis of lipids present in the effluent industrial of an cattle slaughter industry. The treatment system used was composed of two separate reactors: one being the anaerobic fluidized bed reactor (AFBR), inoculated with immobilized enzymes on the matrix support, and the other by sequential batch reactor (SBR) inoculated with activated sludge. Whereas, the reactors have been developed and installed at the Wastewater Treatment Laboratory, Faculdade de Ciências e Tecnologia, UNESP, campus Presidente Prudente. The procedure operating occurred differently for each reactor: preparation and inoculation of enzyme granules, filling the reactor, hydrolysis, and AFBR emptying, filling, aerobic reaction, sedimentation, and emptying the SBR. We performed three experimental stages, with the first and second stage of the work were done reactor analyzes separately, and the third step of the analysis were made with the interconnected reactors... (Complete abstract electronic access below)

Jejum no pré-abate de suínos: bem estar animal e qualidade da carne

In the pre-slaughter management of pigs there are stressors, which depending on the duration or severity, are able to alter the quality of meat. One of the factors considered critical to the welfare is the fasting period in the farm. This practice is fundamental and proven relevance in the production chain. Besides the effects on meat quality and welfare of pigs, fasting is among the main factors responsible for the incidence of skin lesions, can significantly influence the incidence of esophagogastric ulcers and is also important to avoid any contamination of carcasses by gastrointestinal contents. When fasting is done right, it has a positive impact on welfare, meat quality and conditions of welfare during the pre-slaughter management of pigs

Mediadores da via intrínseca da morte celular programada relacionados à infecção in vitro pelo herpesvirus bovino tipo 5

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização e infecção de células tronco mesenquimais e células semelhantes a neurônios, derivadas de cordão umbilical bovino pelo herpesvírus bovino Tipo 5 (BoHV-5)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise histomorfológica e molecular de lesões granulomatosas sugestivas de tuberculose bovina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de imunossensor baseado na imobilização de anticorpo monoclonal em fibroína da seda para diagnóstico rápido da cisticercose bovina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aspectos morfológicos e funcionais do corpo lúteo bovino durante a luteólise parcial e total

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito de vernizes fluoretados suplementados com trimetafosfato de sódio na desmineralização e remineralização do esmalte bovino: estudos in vitro e in situ

Pós-graduação em Ciência Odontólogica - FOA

Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Utilização de informações genômicas para o melhoramento genético de características da carne em bovinos da raça Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV

Desempenho de bovinos cruzados e parâmetros qualitativos de músculos maturados

Pós-graduação em Zootecnia - FCAV

Mineralização de nitrogênio do esterco bovino e produção de alface em função de N-ureia

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Efeito de nanopartículas de trimetafosfato de sódio associadas a dentifrício com concentração reduzida de fluoreto sobre a desmineralização do esmalte bovino: estudos in vitro e in situ

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)