La sinrazón de mi vida : Ficción autobiográfica, relato antiestatal y peronismo en La madriguera de Tununa Mercado

La presente investigación se enmarca en el proyecto mayor de nuestro plan de Doctorado en Letras. Luego de realizar una breve presentación de la obra de Tununa Mercado, procedemos a plantear nuestra hipótesis de trabajo: la de que a partir de la publicación de su libro La madriguera (1996) una ficción autobiográfica de infancia (categoría que involucra variables cuya fiabilidad han sido fuertemente sospechadas y cuestionadas por líneas teóricas desde el giro lingüístico en adelante) la autora funda un modo de réplica literaria (Avellaneda, 1983) al gobierno menemista y su ideología, bajo el procedimiento de una operación sustitutoria, desviada, en la que alude al gobierno de Juan Domingo Perón. Narrando y aludiendo a ese gobierno, desestabiliza las ficciones estatales oficiales (en especial el texto de lectura obligatoria La razón de mi vida, 1951) que sirvieron para legitimarlo y producir cohesión política y social, al tiempo que realiza una respuesta cultural de resistencia a la ideología y las prácticas sociales menemistas. Al mismo tiempo, construye un sujeto autobiográfico femenino, lo que involucra operaciones fuertemente innovadoras en orden de imponer una torsión en un género de fuerte tradición patriarcal en Occidente (Klein, 2001). Como toda ficción del origen, La madriguera no escapa a ciertos relatos míticos, fundantes de la identidad, en especial de carácter letrado, e interroga las categorías de la percepción, fundando una nueva sensibilidad y problematizando así el dominio de lo real. Esta es su forma de dar cuenta de los conflictos de fundamento social y no la que opera a partir de una doxa tranquilizadora y deformadora propia de los relatos estatales.

La sinrazón de mi vida : Ficción autobiográfica, relato antiestatal y peronismo en La madriguera de Tununa Mercado

La presente investigación se enmarca en el proyecto mayor de nuestro plan de Doctorado en Letras. Luego de realizar una breve presentación de la obra de Tununa Mercado, procedemos a plantear nuestra hipótesis de trabajo: la de que a partir de la publicación de su libro La madriguera (1996) una ficción autobiográfica de infancia (categoría que involucra variables cuya fiabilidad han sido fuertemente sospechadas y cuestionadas por líneas teóricas desde el giro lingüístico en adelante) la autora funda un modo de réplica literaria (Avellaneda, 1983) al gobierno menemista y su ideología, bajo el procedimiento de una operación sustitutoria, desviada, en la que alude al gobierno de Juan Domingo Perón. Narrando y aludiendo a ese gobierno, desestabiliza las ficciones estatales oficiales (en especial el texto de lectura obligatoria La razón de mi vida, 1951) que sirvieron para legitimarlo y producir cohesión política y social, al tiempo que realiza una respuesta cultural de resistencia a la ideología y las prácticas sociales menemistas. Al mismo tiempo, construye un sujeto autobiográfico femenino, lo que involucra operaciones fuertemente innovadoras en orden de imponer una torsión en un género de fuerte tradición patriarcal en Occidente (Klein, 2001). Como toda ficción del origen, La madriguera no escapa a ciertos relatos míticos, fundantes de la identidad, en especial de carácter letrado, e interroga las categorías de la percepción, fundando una nueva sensibilidad y problematizando así el dominio de lo real. Esta es su forma de dar cuenta de los conflictos de fundamento social y no la que opera a partir de una doxa tranquilizadora y deformadora propia de los relatos estatales.

Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques, supplementary material

Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes using confocal and multiphoton scanning microscopy, which are increasingly employed as non-invasive approach to physiological in-vivo and ex-vivo studies. Here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of reactive oxygen (ROS) and nitrogen (RNS) species formation in the living tissue. Our results indicate that H2O2, HOO. and ONOO- radicals (assessed through C-H2DFFDA staining) are mainly formed within the blood sinus of the filaments and are likely to be produced by hemocytes as defense against invading pathogens. The oxidative damage in these areas is controlled by enhanced CAT (catalase) activities recorded within the filaments. The outermost areas of the ciliated epithelial cells composing the filaments, concentrated the highest mitochondrial densities (MTK Deep Red 633 staining) and the most acidic pH values (as observed with ageladine-a). These mitochondria have low (depolarized) membrane potentials (D psi m) (JC-1 staining), suggesting that the high amounts of ATP required for ciliary beating may be in part produced by non-mitochondrial mechanisms, such as the enzymatic activity of an ATP-regenerating kinase. Nitric oxide (NO, DAF-2DA staining) produced in the region of the peripheral mitochondria may have an effect on mitochondrial electron transport and possibly cause the low membrane potential. High DAF-2DA staining was moreover observed in the muscle cells composing the wall of the blood vessels where NO may be involved in regulating blood vessel diameter. On the ventral bend of the gills, subepithelial mucus glands (SMG) contain large mucous vacuoles showing higher fluorescence intensities for O2.- (DHE staining) than the rest of the tissue. Given the antimicrobial properties of superoxide, release of O2.- into the mucus may help to avoid the development of microbial biofilms on the gill surface. However, cells of the ventral bends are paying a price for this antimicrobial protection, since they show significantly higher oxidative damage, according to the antioxidant enzyme activities and the carbonyl levels, than the rest of the gill tissue. This study provides the first evidence that one single epithelial cell may contain mitochondria with significantly different membrane potentials. Furthermore, we provide new insight into ROS and RNS formation in ex-vivo gill tissues which opens new perspectives for unraveling the different ecophysiological roles of ROS and RNS in multifunctional organs such as gills.

(Appendix) Vertebral number of topsmelt, Atherinops affinis, adults and offspring from different latitudes along the North-American Pacific coast

Organisms that are distributed across spatial climate gradients often exhibit adaptive local variations in morphological and physiological traits, but to what extent such gradients shape evolutionary responses is still unclear. Given the strong natural contrast in latitudinal temperature gradients between the North-American Pacific and Atlantic coast, we asked how increases in vertebral number (VN, known as Jordan's Rule) with latitude would differ between Pacific (Atherinops affinis) and Atlantic Silversides (Menidia menidia), two ecologically equivalent and taxonomically similar fishes with similar latitudinal distributions. VN was determined from radiographs of wild-caught adults (genetic + environmental differences) and its genetic basis confirmed by rearing offspring in common garden experiments. Compared to published data on VN variation in M. menidia (a mean increase of 7.0 vertebrae from 32 to 46°N, VN slope = 0.42/lat), the latitudinal VN increase in Pacific Silversides was approximately half as strong (a mean increase of 3.3 vertebrae from 28 to 43°N, VN slope = 0.23/lat). This mimicked the strong Atlantic (1.11°C/lat) versus weak Pacific latitudinal gradient (0.40°C/lat) in median annual sea surface temperature (SST). Importantly, the relationship of VN to SST was not significantly different between the two species (average slope = -0.39 vertebrae/°C), thus suggesting a common thermal dependency of VN in silverside fishes. Our findings provide novel support for the hypothesis that temperature gradients are the ultimate cause of Jordan's Rule, even though its exact adaptive significance remains speculative. A second investigated trait, the mode of sex determination in Atlantic versus Pacific Silversides, revealed patterns that were inconsistent with our expectation: M. menidia displays temperature-dependent sex determination (TSD) at low latitudes, where growing seasons are long or unconstrained, but also a gradual shift to genetic sex determination (GSD) with increasing latitude due to more and more curtailed growing seasons. Sex ratios in A. affinis, on the other hand, were independent of latitude and rearing temperature (indicating GSD), even though growing seasons are thermally unconstrained across most of the geographical distribution of A. affinis. This suggests that additional factors (e.g., longevity) play an important role in shaping the mode of sex determination in silverside fishes.

Documentation of sediment cores from the Great Meteor Seamount, North Atlantic

Sedimentological and biostratigraphic investigations of 15 cores (total length: 88 m) from the vicinity of Great Meteor seamount (about 30° N, 28° W) showed that the calcareous ooze are asymmetrically distributed around the seamount and vertically differentiated into two intervals. East and west of the seampunt, the upper "A"-interval is characterized by yellowish-brown sediment colors and bioturbation; ash layers and diatoms are restricted to the eastern cores. On both seamount flanks, the sediment of the lower "B"-interval are white and very rich in CaCO3 with a major fine silt (2-16 µ) mode (mainly coccoliths). Lamination, manganese micronodules, Tertiary foraminifera and discoasters, and small limestone and basalt fragments are typical of the "B"-interval of the eastern cores only. The sediments contain abundant displaced material which was reworked from the upper parts of the seamount. The sedimentation around the seamount is strongly influenced by the kind of displaced material and the intensity of its differentiated dispersal: the sedimentation rates are generally higher on the east than on the west flank /e.g. in "B": 0.9 cm/1000 y in the W; 3.1 cm/1000 y in the E), and lower for the "A" than for the "B"-interval. The lamination is explained by the combination of increased sedimentation rates with a strong input of material poor in organic carbon producing a hostile environment for benthic life. The CaCO3 content of the core is highly influenced by the proportion of displaced bigenous carbonate material (mainly coccoliths). The genuine in-situ conditions of the dissolution facies are only reflected by the minimum CaCO3 values of the cores (CCD = about 5,500 m; first bend in dissolution curve = 4,000 m; ACD = about 3,400 m). The preservation of the total foraminiferal association depends on the proportions of in-situ versus displaced specimens. In greater water depths (stronger dissolution), for example, the preservation can be improved by the admixture of relatively well preserved displaced foraminifera. Carbonate cementation and the formation of manganese micronodules are restricted to microenvironments with locally increased organic carbon contents (e.g. pellets; foraminifera). The ash layers consist of redeposited, silicic volcanic glass of trachytic composition and Mio-Pliocene age; possibly, they can be derived from the upper part of the seamount. Siliceous organisms, especially diatoms, are frequent close to the ash layers and probably also redeposited. Their preservation was favoured by the increase of the SiO2 content in the pore water caused by the silicic volcanic glass. The cores were biostraftsraphically subdivided with the aid of planktonic foraminifera and partly alsococcoliths. In most cases, the biostratigraphically determined cold- and warm sections could be correlated from core to core. Almost all cores do not penetrate the Late Pleistocene. All Tertiary fossils are reworked. In general, the warm/cold boundary W2/C2 corresponds with the lithostratigraphic A/B boundray. Benthonic foraminifera indicate the original site deposition of the displaced material (summit plateau or flanks of the seamount). The asymmetric distribution of the sediments around the seamount east and west of the NE-directed antarctic bottom current (AABW) is explained by the distortion of the streamlines by the Coriolis force; by this process the current velocity is increased west of the seamount and decreased east of it. The different proportion of displaced material within the "A" and "B" interval is explained by changes of the intensity of the oceanic circulation. At the time of "B" the flow of the AABW around the seamount was stronger than during "A"; this can be inferred from the presence of characteristic benthonic foraminifera. The increased oceanic circulation implies an enhanced differentiation of the current velocities, and by that, also of the sedimentation rates, and intensifies the winnowed sediment material was transported downslope by turbid layers into the deep-sea, incorporated into the current system of the AABW, and asymmetrically deposited around the seamount.

Age determination and Argon geochemistry of Hawaiian Emperor seamounts

The Hawaiian-Emperor bend has played a prominent yet controversial role in deciphering past Pacific plate motions and the tempo of plate motion change. New ages for volcanoes of the central and southern Emperor chain define large changes in volcanic migration rate with little associated change in the chain's trend, which suggests that the bend did not form by slowing of the Hawaiian hot spot. Initiation of the bend near Kimmei seamount about 50 million years ago (MA) was coincident with realignment of Pacific spreading centers and early magmatism in western Pacific arcs, consistent with formation of the bend by changed Pacific plate motion.

The formation of a subsurface anticyclonic eddy in the Peru-Chile Undercurrent and its impact on the near-coastal salinity, oxygen and nutrient distributions

The formation of a subsurface anticyclonic eddy in the Peru-Chile Undercurrent (PCUC) in January and February 2013 is investigated using a multi-platform four-dimensional observational approach. Research vessel, multiple glider and mooring-based measurements were conducted in the Peruvian upwelling regime near 12°30'S. The dataset consists of more than 10000 glider profiles and repeated vessel-based hydrography and velocity transects. It allows a detailed description of the eddy formation and its impact on the near-coastal salinity, oxygen and nutrient distributions. In early January, a strong PCUC with maximum poleward velocities of ca. 0.25 m/s at 100 to 200 m depth was observed. Starting on January 20 a subsurface anticyclonic eddy developed in the PCUC downstream of a topographic bend, suggesting flow separation as the eddy formation mechanism. The eddy core waters exhibited oxygen concentrations less than 1mol/kg, an elevated nitrogen-deficit of ca. 17µmol/l and potential vorticity close to zero, which seemed to originate from the bottom boundary layer of the continental slope. The eddy-induced across-shelf velocities resulted in an elevated exchange of water masses between the upper continental slope and the open ocean. Small scale salinity and oxygen structures were formed by along-isopycnal stirring and indications of eddy-driven oxygen ventilation of the upper oxygen minimum zone were observed. It is concluded that mesoscale stirring of solutes and the offshore transport of eddy core properties could provide an important coastal open-ocean exchange mechanism with potentially large implications for nutrient budgets and biogeochemical cycling in the oxygen minimum zone off Peru.

Control rod drop surveillance using two friction coefficients

In the case of large burnup, a control rod (CR) guide tube in the pressurized water reactor of a commercial nuclear power plant might bend. As a consequence, a CR drop experiment may indicate an event of a CR partially inserted and whether the CR should be deemed inoperable. Early prevention of such an event can be achieved by measuring two friction coefficients: the hydraulic coefficient and the sliding coefficient. The hydraulic coefficient hardly changes, so that the curvature of the guide tube can only be detected thanks to a variation of the sliding coefficient. A simple model for the CR drop is established and validated with CR drop experiments. If tmx denotes the instant of CR maximum velocity, a linear relationship between (tmx)_2 and the sliding coefficient is found.

BR3: a biologically inspired fish-like robot actuated by SMA-based artificial muscles

Los peces son animales, donde en la mayoría de los casos, son considerados como nadadores muy eficientes y con una alta capacidad de maniobra. En general los peces se caracterizan por su capacidad de maniobra, locomoción silencioso, giros y partidas rápidas y viajes de larga distancia. Los estudios han identificado varios tipos de locomoción que los peces usan para generar maniobras y natación constante. A bajas velocidades la mayoría de los peces utilizan sus aletas pares y / o impares para su locomoción, que ofrecen una mayor maniobrabilidad y mejor eficiencia de propulsión. A altas velocidades la locomoción implica el cuerpo y / o aleta caudal porque esto puede lograr un mayor empuje y aceleración. Estas características pueden inspirar el diseo y fabricación de una piel muy flexible, una aleta caudal mórfica y una espina dorsal no articulada con una gran capacidad de maniobra. Esta tesis presenta el desarrollo de un novedoso pez robot bio-inspirado y biomimético llamado BR3, inspirado en la capacidad de maniobra y nado constante de los peces vertebrados. Inspirado por la morfología de los peces Micropterus salmoides o también conocido como lubina negra, el robot BR3 utiliza su fundamento biológico para desarrollar modelos y métodos matemáticos precisos que permiten imitar la locomoción de los peces reales. Los peces Largemouth Bass pueden lograr un nivel increíble de maniobrabilidad y eficacia de la propulsión mediante la combinación de los movimientos ondulatorios y aletas morficas. Para imitar la locomoción de los peces reales en una contraparte artificial se necesita del análisis de tecnologías de actuación alternativos, como arreglos de fibras musculares en lugar de servo actuadores o motores DC estándar, así como un material flexible que proporciona una estructura continua sin juntas. Las aleaciones con memoria de forma (SMAs) proveen la posibilidad de construir robots livianos, que no emiten ruido, sin motores, sin juntas y sin engranajes. Asi es como un pez robot submarino se ha desarrollado y cuyos movimientos son generados mediante SMAs. Estos actuadores son los adecuados para doblar la espina dorsal continua del pez robot, que a su vez provoca un cambio en la curvatura del cuerpo. Este tipo de arreglo estructural está inspirado en los músculos rojos del pescado, que son usados principalmente durante la natación constante para la flexión de una estructura flexible pero casi incompresible como lo es la espina dorsal de pescado. Del mismo modo la aleta caudal se basa en SMAs y se modifica para llevar a cabo el trabajo necesario. La estructura flexible proporciona empuje y permite que el BR3 nade. Por otro lado la aleta caudal mórfica proporciona movimientos de balanceo y guiada. Motivado por la versatilidad del BR3 para imitar todos los modos de natación (anguilliforme, carangiforme, subcarangiforme y tunniforme) se propone un controlador de doblado y velocidad. La ley de control de doblado y velocidad incorpora la información del ángulo de curvatura y de la frecuencia para producir el modo de natación deseado y a su vez controlar la velocidad de natación. Así mismo de acuerdo con el hecho biológico de la influencia de la forma de la aleta caudal en la maniobrabilidad durante la natación constante se propone un control de actitud. Esta novedoso robot pescado es el primero de su tipo en incorporar sólo SMAs para doblar una estructura flexible continua y sin juntas y engranajes para producir empuje e imitar todos los modos de natación, así como la aleta caudal que es capaz de cambiar su forma. Este novedoso diseo mecatrónico presenta un futuro muy prometedor para el diseo de vehículos submarinos capaces de modificar su forma y nadar mas eficientemente. La nueva metodología de control propuesto en esta tesis proporcionan una forma totalmente nueva de control de robots basados en SMAs, haciéndolos energéticamente más eficientes y la incorporación de una aleta caudal mórfica permite realizar maniobras más eficientemente. En su conjunto, el proyecto BR3 consta de cinco grandes etapas de desarrollo: • Estudio y análisis biológico del nado de los peces con el propósito de definir criterios de diseño y control. • Formulación de modelos matemáticos que describan la: i) cinemática del cuerpo, ii) dinámica, iii) hidrodinámica iv) análisis de los modos de vibración y v) actuación usando SMA. Estos modelos permiten estimar la influencia de modular la aleta caudal y el doblado del cuerpo en la producción de fuerzas de empuje y fuerzas de rotación necesarias en las maniobras y optimización del consumo de energía. • Diseño y fabricación de BR3: i) estructura esquelética de la columna vertebral y el cuerpo, ii) mecanismo de actuación basado en SMAs para el cuerpo y la aleta caudal, iii) piel artificial, iv) electrónica embebida y v) fusión sensorial. Está dirigido a desarrollar la plataforma de pez robot BR3 que permite probar los métodos propuestos. • Controlador de nado: compuesto por: i) control de las SMA (modulación de la forma de la aleta caudal y regulación de la actitud) y ii) control de nado continuo (modulación de la velocidad y doblado). Está dirigido a la formulación de los métodos de control adecuados que permiten la modulación adecuada de la aleta caudal y el cuerpo del BR3. • Experimentos: está dirigido a la cuantificación de los efectos de: i) la correcta modulación de la aleta caudal en la producción de rotación y su efecto hidrodinámico durante la maniobra, ii) doblado del cuerpo para la producción de empuje y iii) efecto de la flexibilidad de la piel en la habilidad para doblarse del BR3. También tiene como objetivo demostrar y validar la hipótesis de mejora en la eficiencia de la natación y las maniobras gracias a los nuevos métodos de control presentados en esta tesis. A lo largo del desarrollo de cada una de las cinco etapas, se irán presentando los retos, problemáticas y soluciones a abordar. Los experimentos en canales de agua estarán orientados a discutir y demostrar cómo la aleta caudal y el cuerpo pueden afectar considerablemente la dinámica / hidrodinámica de natación / maniobras y cómo tomar ventaja de la modulación de curvatura que la aleta caudal mórfica y el cuerpo permiten para cambiar correctamente la geometría de la aleta caudal y del cuerpo durante la natación constante y maniobras. ABSTRACT Fishes are animals where in most cases are considered as highly manoeuvrable and effortless swimmers. In general fishes are characterized for his manoeuvring skills, noiseless locomotion, rapid turning, fast starting and long distance cruising. Studies have identified several types of locomotion that fish use to generate maneuvering and steady swimming. At low speeds most fishes uses median and/or paired fins for its locomotion, offering greater maneuverability and better propulsive efficiency At high speeds the locomotion involves the body and/or caudal fin because this can achieve greater thrust and accelerations. This can inspire the design and fabrication of a highly deformable soft artificial skins, morphing caudal fins and non articulated backbone with a significant maneuverability capacity. This thesis presents the development of a novel bio-inspired and biomimetic fishlike robot (BR3) inspired by the maneuverability and steady swimming ability of ray-finned fishes (Actinopterygii, bony fishes). Inspired by the morphology of the Largemouth Bass fish, the BR3 uses its biological foundation to develop accurate mathematical models and methods allowing to mimic fish locomotion. The Largemouth Bass fishes can achieve an amazing level of maneuverability and propulsive efficiency by combining undulatory movements and morphing fins. To mimic the locomotion of the real fishes on an artificial counterpart needs the analysis of alternative actuation technologies more likely muscle fiber arrays instead of standard servomotor actuators as well as a bendable material that provides a continuous structure without joins. The Shape Memory Alloys (SMAs) provide the possibility of building lightweight, joint-less, noise-less, motor-less and gear-less robots. Thus a swimming underwater fish-like robot has been developed whose movements are generated using SMAs. These actuators are suitable for bending the continuous backbone of the fish, which in turn causes a change in the curvature of the body. This type of structural arrangement is inspired by fish red muscles, which are mainly recruited during steady swimming for the bending of a flexible but nearly incompressible structure such as the fishbone. Likewise the caudal fin is based on SMAs and is customized to provide the necessary work out. The bendable structure provides thrust and allows the BR3 to swim. On the other hand the morphing caudal fin provides roll and yaw movements. Motivated by the versatility of the BR3 to mimic all the swimming modes (anguilliform, caranguiform, subcaranguiform and thunniform) a bending-speed controller is proposed. The bending-speed control law incorporates bend angle and frequency information to produce desired swimming mode and swimming speed. Likewise according to the biological fact about the influence of caudal fin shape in the maneuverability during steady swimming an attitude control is proposed. This novel fish robot is the first of its kind to incorporate only SMAs to bend a flexible continuous structure without joints and gears to produce thrust and mimic all the swimming modes as well as the caudal fin to be morphing. This novel mechatronic design is a promising way to design more efficient swimming/morphing underwater vehicles. The novel control methodology proposed in this thesis provide a totally new way of controlling robots based on SMAs, making them more energy efficient and the incorporation of a morphing caudal fin allows to perform more efficient maneuvers. As a whole, the BR3 project consists of five major stages of development: • Study and analysis of biological fish swimming data reported in specialized literature aimed at defining design and control criteria. • Formulation of mathematical models for: i) body kinematics, ii) dynamics, iii) hydrodynamics, iv) free vibration analysis and v) SMA muscle-like actuation. It is aimed at modelling the e ects of modulating caudal fin and body bend into the production of thrust forces for swimming, rotational forces for maneuvering and energy consumption optimisation. • Bio-inspired design and fabrication of: i) skeletal structure of backbone and body, ii) SMA muscle-like mechanisms for the body and caudal fin, iii) the artificial skin, iv) electronics onboard and v) sensor fusion. It is aimed at developing the fish-like platform (BR3) that allows for testing the methods proposed. • The swimming controller: i) control of SMA-muscles (morphing-caudal fin modulation and attitude regulation) and ii) steady swimming control (bend modulation and speed modulation). It is aimed at formulating the proper control methods that allow for the proper modulation of BR3’s caudal fin and body. • Experiments: it is aimed at quantifying the effects of: i) properly caudal fin modulation into hydrodynamics and rotation production for maneuvering, ii) body bending into thrust generation and iii) skin flexibility into BR3 bending ability. It is also aimed at demonstrating and validating the hypothesis of improving swimming and maneuvering efficiency thanks to the novel control methods presented in this thesis. This thesis introduces the challenges and methods to address these stages. Waterchannel experiments will be oriented to discuss and demonstrate how the caudal fin and body can considerably affect the dynamics/hydrodynamics of swimming/maneuvering and how to take advantage of bend modulation that the morphing-caudal fin and body enable to properly change caudal fin and body’ geometry during steady swimming and maneuvering.

Corteza y gravedad: el plano del suelo como estrategia en la arquitectura contemporánea

Es, en el encuentro de los edificios con el terreno, donde el suelo como realidad se transforma en cualidad arquitectónica. La presente tesis aborda el estudio del plano del suelo, haciendo una revisión crítica de la arquitectura como mecanismo de pensamiento proyectual. Este análisis se enmarca a partir de los años sesenta, por considerar que es cuando comienza a evidenciarse la ruptura respecto a la herencia del Movimiento Moderno. Es entonces cuando la arquitectura marca un punto de inflexión, y empiezan a surgir diferentes actitudes metodológicas respecto al suelo, totalmente nuevas. Las clásicas acciones de encuentro como posar, elevar o enterrar fueron poco a poco sustituidas por otras más complejas como plegar, inclinar o esponjar. Utilizando como marco de restricción los encuentros o desencuentros del objeto arquitectónico con el terreno, se analiza el suelo como estrategia arquitectónica tratando de demostrar como su manipulación puede ser una eficaz herramienta con la que establecer relaciones específicas con el lugar. La capacidad que presenta el suelo, como elemento arquitectónico, de explorar y modificar las características de cada entorno, hacen de esta superficie una eficiente forma de contextualización. Por tanto, la manipulación del suelo que aquí se plantea, opera transcodificando los elementos específicos de cada lugar y actúa como estrategia arquitectónica que pone en relación al edificio con el contexto, modificando las particularidades formales de dicho plano. Frente a la tendencia que reduce la expresión arquitectónica a una simple apariencia formal autónoma, se plantea la manipulación del plano del suelo como mecanismo de enraizamiento con el lugar, enfatizando para ello la condición terrestre de la arquitectura. El plano del suelo es el que ata al edificio mediante la gravedad a la corteza terrestre. En realidad se trata de realzar el carácter mediador del suelo en la arquitectura, definiendo para ello el establecimiento de elementos comunes entre realidades distintas, potenciando el valor del suelo como herramienta que puede transcodificar el entorno, trasformando su datos en elementos arquitectónicos concretos. En este proceso de traducción de información, el suelo pasa de ser un objeto pasivo a ser uno operativo, convirtiéndose en parte activa de las acciones que sobre él se ejercen. La tesis tiene también como propósito demostrar cómo, la clave de la rápida evolución que el suelo como estrategia arquitectónica ha sufrido en los últimos años, mucho debe a la expansión del suelo en otras artes como en la escultura, y mas concretamente en el landart. Surgen entonces nuevas disciplinas, en las que se propone la comprensión del lugar en los proyectos desarrollando una visión integral del mundo natural, convirtiéndolo en un tejido viviente y conector que pone en relación las actividades que sustenta. También encontramos en Utzon, y sus plataformas geológicas, al precursor de la importancia que más tarde se le daría al plano del suelo en la arquitectura, ya que inicia cierta actitud crítica, que hizo avanzar hacia una arquitectura más expresiva que requería nuevos mecanismos que la relacionasen con el suelo que los soportaba, proponiendo con sus plataformas una transformación infraestructural del suelo. Con su interpretación transcultural de las estructuras espaciales arquetípicas mayas, chinas y japonesas, irá enriqueciendo el panorama arquitectónico, adquiriendo de este modo más valor el contexto que acabará por ser entendido de forma más compleja. Los proyectos de arquitectura en muchas ocasiones se han convertido en territorios propicios de especulación donde construir teoría arquitectónica. Desde este contexto se analizan cuatro estrategias de suelo a través del estudio de cuatro posiciones arquitectónicas muy significativas desde el punto de vista de la manipulación del plano del suelo, que construyen una interesante metodología proyectual con la que operar. Los casos de estudio, propuestos son; la Terminal Pasajeros (1996-2002) en Yokohama del estudio FOA, la Casa de la Música (1999-2005) de OMA en Oporto, el Memorial Judío (1998-2005) de Berlín de Peter Eisenman, y por último el Museo MAXXI (1998-2009) de Zaha Hadid en Roma. Descubrir las reglas, referencias y metodologías que cada uno nos propone, nos permite descubrir cuáles son los principales posicionamientos en relación al proyecto y su relación con el lugar. Las propuestas aquí expuestas abordan una nueva forma de entender el suelo, que hizo avanzar a la arquitectura hacia nuevos modos de encuentro con el terreno. Nos permite también establecer cuáles son las principales aportaciones arquitectónicas del suelo, como estrategia arquitectónica, que han derivado en su reformulación. Dichas contribuciones abren nuevas formas de abordar la arquitectura basadas en el movimiento y en la flexibilidad funcional, o en la superposición de flujos de información y circulación. También plantean nuevas vías desdibujando la figura contra el fondo, y refuerzan la idea del suelo como plataforma infraestructural que ya había sido enunciada por Utzon. Se trata en definitiva de proponer la exploración de la superficie del suelo como el elemento más revelador de las formas emergentes del espacio. ABSTRACT Where the building hits the ground, it is where the latter as reality becomes architectural quality. This thesis presents the study of the ground plane, making a critical review of architecture as a mechanism of projectual thought. This analysis starts from the sixties, because it is when the break begins to be demonstrated with regard to the inheritance of the Modern Movement. It is then when architecture marks a point of inflexion, and different, completely new methodological attitudes to the ground start to emerge. The classic meeting action like place, raise or bury are gradually replaced by more complex operations such as fold, bend or fluff up. Framing it within the meetings or disagreements between architectural object and ground, we analyzed it as architectural strategy trying to show how handling can be an effective tool to establish a specific relationship with the place. The capacity ground has, as an architectural element, to explore and modify the characteristics of each environment, makes this area an efficient tool for contextualization. Therefore, the manipulation of ground that is analyzed here, operates transcoding the specifics of each place and acts as architectural strategy that relates to the building with the context, modifying the structural peculiarities of such plane. Opposite to the reductive tendency of the architectural expression to a simple formal autonomous appearance, the manipulation of the ground plane is considered as a rooting mechanism place that emphasises the earthly condition of architecture. The ground plane is the one that binds the building by gravity to the earth’s crust. In fact, it tries to study the mediating character of the ground in architecture, defining for it to establish commonalities among different realities, enhancing the value of the ground as a tool that can transcode the environment, transforming its data into specific architectural elements. In this process of translating information, the ground goes from being a liability, to become active part of the actions exerted on the object. The thesis also tries to demonstrate how the key of the rapid evolution that the ground likes architectural strategy has gone through recently, much due to its use expansion in other arts such as sculpture. New disciplines arise then, in which one proposes the local understanding in the projects, developing an integral vision of the natural world and turning it into an element linking the activities it supports. We also find in Utzon, and his geological platforms, the precursor of the importance that later would be given to the ground plane in architecture, since it initiates a certain critical attitude, which advances towards a more expressive architecture, with new mechanisms that relate to the ground that it sits in, proposing with its platforms an infrastructural transformation of the ground. With his transcultural interpretation of the spatial archetypal structures, he will enrich the architectural discourse, making the context become understood in more complex ways. Architectural projects in many cases have become territories prone to architectural theory speculation. Within this context, four strategies are analyzed through the study of four very significant architectural positions, from the point of view of handling the ground plane, and the project methodology within which to operate. The case studies analyzed are; Passenger Terminal (1996-2002) in Yokohama from FOA, The House of the music (1999-2005) the OMA in Oporto, The Jew monument (1998-2005) in Berlin the Peter Eisenman, and finally the MAXXI Museum (1998-2009) the Zaha Hadid in Rome. Discovering the rules, references and methodologies that each of those offer, it allows to discover what the main positions are regarding the building and its relationship with the place where it is located. The proposals exposed here try to shed a different light on the ground, which architecture advancing in new ways on how meet it. The crossing of the different studied positions, allows us to establish what the main contributions of ground as architectural strategy are. Such contributions open up new approaches to architecture based on movement and functional flexibility, overlapping information flow and circulation, consider new ways that blur the figure against the background, and reinforce the idea of ground as infrastructural platform, already raised by Utzon. Summarizing, it tries to propose the exploration of the ground plane as the most revealing form of spatial exploration.

DNA bending and the initiation of transcription at σ54-dependent bacterial promoters

We have examined the effects on transcription initiation of promoter and enhancer strength and of the curvature of the DNA separating these entities on wild-type and mutated enhancer–promoter regions at the Escherichia coli σ54-dependent promoters glnAp2 and glnHp2 on supercoiled and linear DNA. Our results, together with previously reported observations by other investigators, show that the initiation of transcription on linear DNA requires a single intrinsic or induced bend in the DNA, as well as a promoter with high affinity for σ54-RNA polymerase, but on supercoiled DNA requires either such a bend or a high affinity promoter but not both. The examination of the DNA sequence of all nif gene activator- or nitrogen regulator I-σ54 promoters reveals that those lacking a binding site for the integration host factor have an intrinsic single bend in the DNA separating enhancer from promoter.

Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1

LEF-1 (lymphoid enhancer-binding factor 1) is a cell type-specific member of the family of high mobility group (HMG) domain proteins that recognizes a specific nucleotide sequence in the T cell receptor (TCR) α enhancer. In this study, we extend the analysis of the DNA-binding properties of LEF-1 and examine their contributions to the regulation of gene expression. We find that LEF-1, like nonspecific HMG-domain proteins, can interact with irregular DNA structures such as four-way junctions, albeit with lower efficiency than with specific duplex DNA. We also show by a phasing analysis that the LEF-induced DNA bend is directed toward the major groove. In addition, we find that the interaction of LEF-1 with a specific binding site in circular DNA changes the linking number of DNA and unwinds the double helix. Finally, we identified two nucleotides in the LEF-1-binding site that are important for protein-induced DNA bending. Mutations of these nucleotides decrease both the extent of DNA bending and the transactivation of the TCRα enhancer by LEF-1, suggesting a contribution of protein-induced DNA bending to the function of TCRα enhancer.

Torsional directed walks, entropic elasticity, and DNA twist stiffness

DNA and other biopolymers differ from classical polymers because of their torsional stiffness. This property changes the statistical character of their conformations under tension from a classical random walk to a problem we call the “torsional directed walk.” Motivated by a recent experiment on single lambda-DNA molecules [Strick, T. R., Allemand, J.-F., Bensimon, D., Bensimon, A. & Croquette, V. (1996) Science 271, 1835–1837], we formulate the torsional directed walk problem and solve it analytically in the appropriate force regime. Our technique affords a direct physical determination of the microscopic twist stiffness C and twist-stretch coupling D relevant for DNA functionality. The theory quantitatively fits existing experimental data for relative extension as a function of overtwist over a wide range of applied force; fitting to the experimental data yields the numerical values C = 120 nm and D = 50 nm. Future experiments will refine these values. We also predict that the phenomenon of reduction of effective twist stiffness by bend fluctuations should be testable in future single-molecule experiments, and we give its analytic form.