Audit report on the Hiawatha Water Department, Hiawatha, Iowa for the year ended June 30, 2007

Audit report on the Hiawatha Water Department, Hiawatha, Iowa for the year ended June 30, 2007

Audit report on the City of Shenandoah, Iowa for the year ended June 30, 2007

Audit report on the City of Shenandoah, Iowa for the year ended June 30, 2007

Audit report on the City of Ely, Iowa for the year ended June 30, 2007

Audit report on the City of Ely, Iowa for the year ended June 30, 2007

The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion.

Cell polarization relies on small GTPases, such as Cdc42, which can break symmetry through self-organizing principles, and landmarks that define the axis of polarity. In fission yeast, microtubules deliver the Tea1-Tea4 complex to mark cell poles for growth, but how this complex activates Cdc42 is unknown. Here, we show that ectopic targeting of Tea4 to cell sides promotes the local activation of Cdc42 and cell growth. This activity requires that Tea4 binds the type I phosphatase (PP1) catalytic subunit Dis2 or Sds21, and ectopic targeting of either catalytic subunit is similarly instructive for growth. The Cdc42 guanine-nucleotide-exchange factor Gef1 and the GTPase-activating protein Rga4 are required for Tea4-PP1-dependent ectopic growth. Gef1 is recruited to ectopic Tea4 and Dis2 locations to promote Cdc42 activation. By contrast, Rga4 is locally excluded by Tea4, and its forced colocalization with Tea4 blocks ectopic growth, indicating that Rga4 must be present, but at sites distinct from Tea4. Thus, a Tea4-PP1 landmark promotes local Cdc42 activation and growth both through Cdc42 GEF recruitment and by creating a local trough in a Cdc42 GAP.

Audit report on Clarke Community School District in Osceola, Iowa for the year ended June 30, 2007

Audit report on Clarke Community School District in Osceola, Iowa for the year ended June 30, 2007

Audit report on the Perry Municipal Waterworks, Perry, Iowa for the year ended June 30, 2007

Audit report on the Perry Municipal Waterworks, Perry, Iowa for the year ended June 30, 2007

Audit report on Adair County, Iowa for the year ended June 30, 2007

Audit report on Adair County, Iowa for the year ended June 30, 2007

Audit report on the six divisions of the Iowa Department of Commerce for the year ended June 30, 2006

Audit report on the six divisions of the Iowa Department of Commerce for the year ended June 30, 2006

Audit report on Iowa Public Television for the year ended June 30, 2007

Audit report on Iowa Public Television for the year ended June 30, 2007

Audit report on Sheldon Community School District in Sheldon, Iowa for the year ended June 30, 2007

Audit report on Sheldon Community School District in Sheldon, Iowa for the year ended June 30, 2007

Audit report on the City of Hiawatha, Iowa for the year ended June 30, 2007

Audit report on the City of Hiawatha, Iowa for the year ended June 30, 2007

Audit report on the City of Carlisle, Iowa for the year ended June 30, 2007

Audit report on the City of Carlisle, Iowa for the year ended June 30, 2007

Audit report on the City of Dike, Iowa for the year ended June 30, 2006

Audit report on the City of Dike, Iowa for the year ended June 30, 2006

Audit report on the City of West Branch, Iowa for the year ended June 30, 2007

Audit report on the City of West Branch, Iowa for the year ended June 30, 2007