Yleisradion pääjohtaja Eino S. Repo 3/2 1966

Kirje

The complete amino acid sequence for brain beta spectrin (beta fodrin): relationship to globin sequences.

The amino acid sequence of mouse brain beta spectrin (beta fodrin), deduced from the nucleotide sequence of complementary DNA clones, reveals that this non-erythroid beta spectrin comprises 2363 residues, with a molecular weight of 274,449 Da. Brain beta spectrin contains three structural domains and we suggest the position of several functional domains including f-actin, synapsin I, ankyrin and spectrin self association sites. Analysis of deduced amino acid sequences indicated striking homology and similar structural characteristics of brain beta spectrin repeats beta 11 and beta 12 to globins. In vitro analysis has demonstrated that heme is capable of specific attachment to brain spectrin, suggesting possible new functions in electron transfer, oxygen binding, nitric oxide binding or heme scavenging.

Lhermitte-Duclos disease.

Lhermitte-Duclos disease (dysplastic gangliocytoma of the cerebellum) is a rare pseudo-neoplastic disorder of the cerebellum with typical MRI findings. A 25-year-old man presenting with progressive neck pain, dizziness, and impaired vision is reported. CT and MRI revealed a left cerebellar haemispheric mass and obstructive hydrocephalus. Lhermitte-Duclos disease was histologically confirmed after surgical removal of the lesion. The typical MRI appearance of a nonenhancing haemispheric cerebellar mass with preservation and exaggeration of the normal gyral pattern allows pre-operative diagnosis of this condition. The literature is reviewed and clinical presentation, radiology and histopathology are discussed.

Volúmenes de zooplancton y distribución del ictioplancton en el litoral peruano durante verano 2006

Los volúmenes de zooplancton variaron de 0,3 a 150 mL/m2, en dos núcleos: a) entre Caleta La Cruz y Chimbote y b) al sur de San Juan. Se determinaron 53 familias de ictioplancton, las más frecuentes fueron Engraulidae (31%), Myctophidae (18%) y Photichthyidae (16%). Huevos y larvas de anchoveta se distribuyeron desde caleta La Cruz hasta Matarani. Huevos en concentraciones importantes frente a bahía de Sechura, Salaverry y Supe y larvas frente a Chicama y Supe. La abundancia fluctuó de 3 a 26.385 huevos/m2 (47,7% de frecuencia), y de 3 a 6.210 larvas/m2 (66,4% de frecuencia). Tamaño de larvas varió de 1,5 a 20 mm (recién nacidas a 37 días de vida). La mayor frecuencia de tallas estuvo entre 2 y 4 mm en las áreas B y D, el área C presentó mayor rango de tallas. Huevos y larvas de Vinciguerria lucetia cercanos a la costa de Caleta La Cruz-Paita, (ingreso de Aguas Subtropicales Superficiales) y al sur del Callao. Especies con baja abundancia: merluza, sardina, perico; caballa y jurel, registradas en el norte y centro a excepción de la sardina que se presentó frente a San Juan.

Encuesta de la pesca artesanal en el lago Titicaca (Junio-diciembre 2006)

Entre junio y diciembre 2006, se realizó la encuesta para conocer el estado de la pesca artesanal en el Lago Titicaca, parte peruana. Se encuestaron 130 comunidades pesqueras, contabilizándose 1734 pescadores (93% hombres), 50% menor a lo determinado por PRODUCE-Puno. Se registraron 1716 embarcaciones, en su mayoría de madera que se desplazan a remo y/o vela. Las artes de pesca más utilizadas son redes cortina y espinel. La truchicultura se encuentra en fase de crecimiento.

P. DE L'ESTOILE, « Continuation de mes Mémoires, commençans le premier de l'an 1598 jusques à la fin de febvrier de l'an 1602 ».

Provient des Jacobins de Saint-Honoré.

P. DE L'ESTOILE, « Continuation de mes Mémoires, commençans le premier de l'an 1598 jusques à la fin de febvrier de l'an 1602 ».

Provient des Jacobins de Saint-Honoré.

The effect of fluoxetine on the pharmacokinetics and safety of risperidone in psychotic patients

In this open, 30-day trial, the pharmacokinetics, safety and tolerability of a combination therapy of risperidone (4 or 6 mg/day)and fluoxetine (20mg/day from day 6) were evaluated in 11 psychotic inpatients. CYP2D6 genotyping revealed that 3 and 8 patients were poor metabolizers (PMs) and extensive metabolizers (EMs) of debrisoquine, respectively. The mean (+/- SD) AUC of risperidone increased from 83.1 +/- 46.8 ng.h/ml and 398.3 +/- 33.2 ng.h/ml (monotherapy) to 345.1 +/- 158.0 ng.h/ml (p < 0.05) and 514.0 +/- 144.2 ng.h/ml (p < 0.001) when coadministered with fluoxetine in EMs and PMs, respectively. The AUC of the active moiety (risperidone plus 9-hydroxy-risperidone) increased from 470.0 +/- 170.0 ng.h/ml to 663.0 +/- 243.3 ng.h/ml (p < 0.05)and from 576.3 +/- 19.6 ng.h/ml to 788.0 +/- 89.1 ng.h/ml (ns) in EMs and PMs, respectively. In EMs, the AUC of 9-hydroxy-risperidone remained similar (monotherapy vs. combination therapy: 386.8 +/- 153.0 ng.h/ml vs. 317.7 +/- 125.2 ng.h/ml, ns),whereas it increased in PMs (178.3 +/- 23.5 ng.h/ml vs. 274.0 +/- 55.1 ng.h/ml (p < 0.05)). Ten of the 11 patients showed a clinical improvement (reduction of 20% or more in total PANSS score and 70% on the mean MADRS score compared to baseline). The severity and incidence of extrapyramidal symptoms and adverse events did not significantly increase when fluoxetine was added.

Médecine interne générale ambulatoire: quelques enseignements de la littérature en 2005 [Primary care in general internal medicine: relevant information in 2005]

During the year 2005, the chief residents of the University Medical Outpatient Clinic of Lausanne have done a database of useful articles for daily practice, scientifically validated and with excellent didactic quality, from 10 electronic journals. They have used those selected articles in personal meetings between the chief residents on a regular basis and the possibility to access the database by the junior physicians. Six of these articles concerning different topics (depression, tuberculosis detection, anticoagulation at home, cholinesterase inhibitors, insomnia and therapies, transdermal nitroglycerin and tendinopathies) are presented.

Defining new criteria for selection of cell-based intestinal models using publicly available databases.

BACKGROUND: The criteria for choosing relevant cell lines among a vast panel of available intestinal-derived lines exhibiting a wide range of functional properties are still ill-defined. The objective of this study was, therefore, to establish objective criteria for choosing relevant cell lines to assess their appropriateness as tumor models as well as for drug absorption studies. RESULTS: We made use of publicly available expression signatures and cell based functional assays to delineate differences between various intestinal colon carcinoma cell lines and normal intestinal epithelium. We have compared a panel of intestinal cell lines with patient-derived normal and tumor epithelium and classified them according to traits relating to oncogenic pathway activity, epithelial-mesenchymal transition (EMT) and stemness, migratory properties, proliferative activity, transporter expression profiles and chemosensitivity. For example, SW480 represent an EMT-high, migratory phenotype and scored highest in terms of signatures associated to worse overall survival and higher risk of recurrence based on patient derived databases. On the other hand, differentiated HT29 and T84 cells showed gene expression patterns closest to tumor bulk derived cells. Regarding drug absorption, we confirmed that differentiated Caco-2 cells are the model of choice for active uptake studies in the small intestine. Regarding chemosensitivity we were unable to confirm a recently proposed association of chemo-resistance with EMT traits. However, a novel signature was identified through mining of NCI60 GI50 values that allowed to rank the panel of intestinal cell lines according to their drug responsiveness to commonly used chemotherapeutics. CONCLUSIONS: This study presents a straightforward strategy to exploit publicly available gene expression data to guide the choice of cell-based models. While this approach does not overcome the major limitations of such models, introducing a rank order of selected features may allow selecting model cell lines that are more adapted and pertinent to the addressed biological question.