Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

Improvement of the features of an enzyme is in many instances a pre-requisite for the industrial implementation of these exceedingly interesting biocatalysts. To reach this goal, the researcher may utilize different tools. For example, amination of the enzyme surface produces an alteration of the isoelectric point of the protein along with its chemical reactivity (primary amino groups are the most widely used to obtain the reaction of the enzyme with surfaces, chemical modifiers, etc.) and even its “in vivo” behavior. This review will show some examples of chemical (mainly modifying the carboxylic groups using the carbodiimide route), physical (using polycationic polymers like polyethyleneimine) and genetic amination of the enzyme surface. Special emphasis will be put on cases where the amination is performed to improve subsequent protein modifications. Thus, amination has been used to increase the intensity of the enzyme/support multipoint covalent attachment, to improve the interaction with cation exchanger supports or polymers, or to promote the formation of crosslinkings (both intra-molecular and in the production of crosslinked enzyme aggregates). In other cases, amination has been used to directly modulate the enzyme properties (both in immobilized or free form). Amination of the enzyme surface may also pursue other goals not related to biocatalysis. For example, it has been used to improve the raising of antibodies against different compounds (both increasing the number of haptamers per enzyme and the immunogenicity of the composite) or the ability to penetrate cell membranes. Thus, amination may be a very powerful tool to improve the use of enzymes and proteins in many different areas and a great expansion of its usage may be expected in the near future.

La enseñanza del Derecho del Trabajo a través del método jurídico: análisis normativo, judicial y doctrinal

La presente memoria tiene por objeto exponer las principales conclusiones a las que se ha llegado en relación con la materia de análisis concerniente a los instrumentos docentes que resultan más adecuados en orden a alcanzar con éxito los objetivos relativos a la enseñanza por competencias y el aprendizaje autónomo que permitan al alumno afrontar con éxito el ejercicio de las profesiones para las que le capacita su titulación. El análisis se lleva a cabo en relación con la asignatura Derecho del Trabajo, que constituye una materia central en el Área de Conocimiento del Derecho del Trabajo y de la Seguridad Social, impartida en varias titulaciones universitarias, principalmente, en los estudios de Derecho, DADE, Relaciones Laborales y Recursos Humanos, pero también en los Grados de Turismo, Criminología o Trabajo Social. En este contexto, y partiendo de la base de la importancia y necesidad de diseñar un tipo de formación (y evaluación) por competencias, el equipo investigador de esta red ha trazado el diseño de dicha asignatura a través del recurso a tres herramientas básicas del método jurídico: el análisis de las normas jurídicas, la interpretación judicial de las mismas, y la exégesis efectuada por parte de la doctrina científica.

Fortification and Frontier: The Project Drawn up by Juan Martín Zermeño for Puebla de Sanabria in 1766

Following the death of engineer General Jorge Próspero de Verboom in 1744 and after a few years of transition in the management of Spanish fortifications, Juan Martín Zermeño took on the role, initially with a temporary mandate, but then definitively during a second period that ran from 1766 until his death in 1772. He began this second period with a certain amount of concern because of what had taken place during the last period of conflict. The Seven Years War (1756–1763) which had brought Spain into conflict with Portugal and England in the Caribbean had also lead to conflict episodes along the Spanish–Portuguese border. Zermeño’s efforts as a planner and general engineer gave priority to the northern part of the Spanish–Portuguese border. After studying the territory and the existing fortifications on both sides of the border, Zermeño drew up three important projects in 1766. The outposts that needed to be reinforced were located, from north to south, at Puebla de Sanabria, Zamora and Ciudad Rodrigo, which is where he is believed to have come from. This latter township already had a modern installation built immediately after the war of the Spanish Succession and reinforced with the Fort of La Concepción. However, Zamora and Puebla de Sanabria had some obsolete fortifications that needed modernising. Since the middle of the 15th century Puebla de Sanabria had had a modern castle with rounded turrets, that of the counts of benavente. During the 16th and 17th centuries it had also been equipped with a walled enclosure with small bastions. During the war of the Spanish Succession the Portuguese had enlarged the enclosure and had erected a tentative offshoot to the west. In order to draw up the ambitious Puebla de Sanabria project Zermeño had the aid of some previous reports and projects, such as those by the count of robelin in 1722, the one by Antonio de Gaver in 1752, and Pedro Moreau’s report dated June 1755. This study includes a technical analysis of Zermeño’s project and its strategic position within the system of fortifications along the Spanish–Portuguese border.

Ijāzat Aḥmad ibn Ibrāhīm al-Tūnisī al-Miṣrī li-Muḥammad ibn Shaʻbān al-Ḥalabī : manuscript, 1691

Contains an ijāzah issued by Aḥmad ibn Ibrāhīm al-Tūnisī al-Miṣrī al-Azharī al-Ḥanafī to Muḥammad ibn Shaʻbān ibn Sharaf al-Dīn ibn ʻAbd al-Wahhāb al-Ḥalabī al-Sarmīnī (?) al-Muʻaddil (?) and another one issued by Muḥammad ibn Shaʻbān to two brothers named Ṣāliḥ and ʻAbd al-Raḥmān.

Transfer of Pattern Recall Skills May Contribute to the Development of Sport Expertise

Superior recall of domain-specific patterns is well established as a defining attribute of expert performers. Recent studies on the developmental histories of expert team ball sport players (e.g. Baker, Côté, & Abernethy, 2003a) also suggest that experts characteristically receive exposure to a wide range of sports in their developing years and that this related sports experience may reduce the amount of sport-specific training needed to become an expert. This study examined whether the facilitation of expertise associated with other sport experience might arise from positive transfer of pattern recall skills from one sport to another. Expert netball, basketball and field hockey players and experienced non-experts performed a recall task for patterns of play derived from each of these sports. Experts from sports different to those shown in the presented pattern consistently outperformed non-experts in their recall of defensive player positions, suggesting some selective transfer of pattern recall skills may indeed be possible

Heat flow and heat conductivity coefficient measured in bottom sediments collected in Cruise 13 of R/V Akademik Sergey Vavilov in the Pechora Sea

In Cruise 13 of R/V Akademik Sergey Vavilov in the Pechora Sea, six heat flow varied from 50 to 75 mW/m**2. Deep heat flow in the Pechora Sea was calculated equal to 45 mW/m**2, which is confirmed by results of geological and geophysical studies and corresponds to Middle Baikal age of the basement. A model of structure of the lithosphere in the Pechora Sea is suggested. Total thickness of the lithosphere in the basin (190 km) determined from geothermal data agrees well with that in transition zones from the continent to the ocean. According to estimates of deep heat flow in the region obtained, thickness of the mantle (160 km), of the basaltic (15 km), and of the granitic (15 km) layers of the lithosphere were also evaluated. Temperature values at boundaries of the sedimentary layers were calculated over a geological and geophysical profile crossing the Pechora Sea basin. Temperatures obtained agree with the temperature interval of hydrocarbon generation and correspond to Permian-Triassic sedimentary sequences, which are the most productive ones in the Pechora Sea region from the point of view of oil and gas potential.

Synthesis of existing knowledge and practice in the field of educational partnerships /

"PIP 93-1102"--P. [4] of cover.

Geshichte der Kroaten : Teil 1 /

"Erster Teil (bis 1102)"

Nueua filosofia de la naturaleza del hombre : no conocida, ni alcançada de los grandes filosofos antiguos, la cual mejora la vida y salud humana /

Datos tomados de ICCU.

Segunda parte de los Anales de Flandes /

Mode of access: Internet.

Oghb Edeseay /

Mode of access: Internet.

Memoria que en conformidad con lo que dispone el artículo 24 del nuevo reglamento orgánico para los establecimientos de aguas minerales de 11 de marzo del presente año,elevan los condueños del de las de Benimarfull á la dirección general de Beneficencia y Sanidad del Reyno. /

Acompaña plano:"Copia del establecimiento de los baños de Benimarfull",firmado en Alcoy,el 5 de mayo de 1868,por Francisco Gisbert Payá.En tela.Escala 1:500.29 x 40 cm.

Sancti Hieronymi Stridonensis Operum tomus IIII : continens commentarium in Psalmos & in Epistolas Pauli ac varias epistolas sancto Hieronymo falsò adscriptas /

Index locupletissimus in quatuor tomos operum Sancti Hieronymi quam maxima diligenti collectus : accesit index locorum sacrae scripturae, qui sunt in his Tomis, à diuo Hieronymo explicati.