Purificação e caracterização bioquímica da invertase extracelular produzida pelo fungo filamentoso Aspergillus terreus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de ciclodextrina glicosiltransferase (CGTase) e de ciclodextrinas como coadjuvante na inibição do escurecimento em alimentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise morfológica da superfície dos cones de guta-percha submetidos à desinfecção com hipoclirito de sódio a 1% e a 2,5% e sua influência no selamento marginal da obturação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão da trealose-6-fosfato sintase (TPS) em cana-de-açúcar (Saccharum spp) sob estresse hídrico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da essencialidade da metionina e lisina em frangos de corte pelo turnover de isótopos estáveis de carbono

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de beta-glucanases por Trichoderma harzianum Rifai para obtenção gluco-oligossacarídeos a partir de botriosferana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Glucoamilases mutantes termoestáveis do fungo Aspergillus awamori expressas em levedura Saccharomyces cerevisiae: Sequenciamento do gene, produção e purificação das enzimas obtidas por fermentação submersa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo químico e biológico de espécies de Rubiaceae

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A new flavonoid derivative from leaves of Oxandra Sessiliflora R. E. Fries

A fração em acetato de etila (EtOAc) obtida a partir da partição do extrato de etanol (EtOH) das folhas de O. sessiliflora R. E. Fries (Annonaceae) foi submetida a diversos procedimentos cromatográficos, incluindo cromatografia líquida de alta eficiência (HPLC), o que resultou no isolamento dos flavonóides: quercetina-3-O-α-L-ramnopiranosil-(1→4)-β-D-glucopiranosídeo (1), inédito na literatura, canferol-3-O-α-L-ramnopiranosil-(1→4)-β-D-glucopiranosídeo (2), rutina (3) e canferol-3-O-rutinosídeo (4). As estruturas foram definidas através da análise dos espectros de ressonância magnética nuclear (NMR) de ¹H e de 13C (1D e 2D) e espectrometria de massas.

Endothelial AT(1) and AT(2) pathways in aortic responses to angiotensin II after stress and ethanol consumption in rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Teorias de Spin-1: decomposição em helicidades, redução dimensional e imersão de calibre

We have studied the physical content of the following models: Maxwell, Proca, Self-Dual and Maxwell-Chern-Simons. One method we have used is the decomposition in the so called helicity variables, which can be done in the Lagrangian formalism. It leads to the correct counting of degrees of freedom without choosing a gauge condition. The method separates the propagating modes from the non-propagating ones. The Hamiltonian of the MCS and the AD is calculated. The second method used here is the analysis of the sign of the imaginary part of the residues of the two-point amplitude of the theory, showing that the models analyzed are free of ghosts. We also carry the dimensional reduction of the Maxwell-Chern-Simons and Self-Dual models from D = 2+1 to D = 1 + 1 dimensions. Next, we show that the dimensional reduction of those equivalent models also leads to equivalent models in D=1+1. Even more interesting is the fact, demonstrated here, that those reduced models can also be connected via gauge embedding. So the gauge embedding of the Self-Dual model into the Maxwell-Chern-Simons theory is preserved by the dimensional reduction

Estudo fitoquímico dos extratos de folhas, galhos e cascas do caule de Calycophyllum spruceanum Benth para testes de potencial de cosmético funcional

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fructose-Induced Hypothalamic AMPK Activation Stimulates Hepatic PEPCK and Gluconeogenesis due to Increased Corticosterone Levels

Fructose consumption causes insulin resistance and favors hepatic gluconeogenesis through mechanisms that are not completely understood. Recent studies demonstrated that the activation of hypothalamic 5'-AMP-activated protein kinase (AMPK) controls dynamic fluctuations in hepatic glucose production. Thus, the present study was designed to investigate whether hypothalamic AMPK activation by fructose would mediate increased gluconeogenesis. Both ip and intracerebroventricular (icv) fructose treatment stimulated hypothalamic AMPK and acetyl-CoA carboxylase phosphorylation, in parallel with increased hepatic phosphoenolpyruvate carboxy kinase (PEPCK) and gluconeogenesis. An increase in AMPK phosphorylation by icv fructose was observed in the lateral hypothalamus as well as in the paraventricular nucleus and the arcuate nucleus. These effects were mimicked by icv 5-amino-imidazole-4-carboxamide-1-beta-D-ribofuranoside treatment. Hypothalamic AMPK inhibition with icv injection of compound C or with injection of a small interfering RNA targeted to AMPK alpha 2 in the mediobasal hypothalamus (MBH) suppressed the hepatic effects of ip fructose. We also found that fructose increased corticosterone levels through a mechanism that is dependent on hypothalamic AMPK activation. Concomitantly, fructose-stimulated gluconeogenesis, hepatic PEPCK expression, and glucocorticoid receptor binding to the PEPCK gene were suppressed by pharmacological glucocorticoid receptor blockage. Altogether the data presented herein support the hypothesis that fructose-induced hypothalamic AMPK activation stimulates hepatic gluconeogenesis by increasing corticosterone levels. (Endocrinology 153: 3633-3645, 2012)

Urinary MCP-1 and RBP: Independent predictors of renal outcome in macroalbuminuric diabetic nephropathy

Background: Albuminuria has been considered a sine qua non condition for the diagnosis of diabetic nephropathy (DN) and has been widely used as a surrogate outcome of chronic kidney disease (CKD). However, recent data suggest that albuminuria may fail as a biomarker in a subset of patients, and the search for novel markers is intense. Methods: We analyzed the role of urinary RBP and of serum and urinary cytokines (TGF-beta, MCP-1 and VEGF) as predictors of the risk of dialysis. doubling of serum creatinine or death (primary outcome. PO) in 56 type 2 diabetic patients with macroalbuminuric DN. Results: Mean follow-up time was 30.7 +/- 10 months. Urinary RBP and MCP-1 were significantly higher in patients presenting the PO, whereas no difference was shown for TGF-beta or VEGF. In the Cox regression, urinary RBP. MCP-1 and VEGF were positively associated and serum VEGF was inversely related to the risk of the PO. However, after adjustments for creatinine clearance, proteinuria, and blood pressure only urinary RBP (OR 11.6; 95% CI 2.7-49.2, p = 0.001 for log RBP) and urinary MCP-1 (OR 11.0; 95% CI 1.6-76.4, p = 0.02 for log MCP-1) remained as significant independent predictors of the PO. Conclusion: Urinary RBP and MCP-1 are independently related to the risk of CKD progression in patients with macroalbuminuric DN. Whether these biomarkers have a role in the setting of normoalbuminuria and microalbuminuria in DN should be further investigated. (C) 2012 Elsevier Inc. All rights reserved.

Optimal Timing for Assessment of Tumor Response to Neoadjuvant Chemoradiation in Patients With Rectal Cancer: Do All Patients Benefit From Waiting Longer Than 6 Weeks?

Purpose: To estimate the metabolic activity of rectal cancers at 6 and 12 weeks after completion of chemoradiation therapy (CRT) by 2-[fluorine-18] fluoro-2-deoxy-D-glucose-labeled positron emission tomography/computed tomography ([18 FDG] PET/CT) imaging and correlate with response to CRT. Methods and Materials: Patients with cT2-4N0-2M0 distal rectal adenocarcinoma treated with long-course neoadjuvant CRT (54 Gy, 5-fluouracil-based) were prospectively studied (ClinicalTrials. org identifier NCT00254683). All patients underwent 3 PET/CT studies (at baseline and 6 and 12 weeks fromCRT completion). Clinical assessment was at 12 weeks. Maximal standard uptakevalue (SUVmax) of the primary tumor wasmeasured and recorded at eachPET/CTstudy after 1 h (early) and3 h (late) from 18 FDGinjection. Patientswith an increase in early SUVmax between 6 and 12 weeks were considered " bad" responders and the others as "good" responders. Results: Ninety-one patients were included; 46 patients (51%) were "bad" responders, whereas 45 (49%) patients were " good" responders. " Bad" responders were less likely to develop complete clinical response (6.5% vs. 37.8%, respectively; PZ. 001), less likely to develop significant histological tumor regression (complete or near-complete pathological response; 16% vs. 45%, respectively; PZ. 008) and exhibited greater final tumor dimension (4.3cmvs. 3.3cm; PZ. 03). Decrease between early (1 h) and late (3 h) SUVmax at 6-week PET/CTwas a significant predictor of " good" response (accuracy of 67%). Conclusions: Patients who developed an increase in SUVmax after 6 weeks were less likely to develop significant tumor downstaging. Early-late SUVmax variation at 6-week PET/CT may help identify these patients and allow tailored selection of CRT-surgery intervals for individual patients. (C) 2012 Elsevier Inc.