Representaciones de la vejez y formas del discurso ejemplar romano durante el S. I d.C.

Examinaremos c��mo la vejez en el corpus fedriano se configura a partir de los diálogos que este establece con sus contextos literarios y culturales. Así, la construcción de la ancianidad femenina incorpora estereotipos c��micosatíricos que evidencian la perspectiva romanoc��ntrica desde la que el género se reformula (Phaed.2.2 y 3.1). Por su parte, la debilidad atribuida a los ancianos (Phaed.1.15 y 1.21) -leída en contraposición con la fortaleza que su contemporáneo, Valerio Máximo, adjudica a la ancianidad mod��lica en sus exempla (V. Max. 8.13)- contribuye a la construcción de la máscara del fabulista como sujeto marginal (3. 9 y 3. epil.).

Historia de la provincia de la corona de Aragon de la... orden de los Ermitaños de... San Agustin... : parte II, III, y IV, contienen las fundaciones de los conventos de religiosos ...

Sign.: []2, 2[calderón]4, A-Z4, 2A-2Z4, 3A-3Q4, 3R2

Decada legal, en que contrahidas a diez las Leyes de estos Reynos, se van poniendo por resumen, y baxo un contexto las de cada parte de la Decada : Tomo primero ; que contiene una idea de la legislacion ... mostrando las epocas de su formacion ... con las leyes Iª, IIª, IIIª y IVª de ella

Contiene: Leyes religiosa y regia de Castilla : primera y segunda de la Decada ; Leyes Magistratoria y Popular : tercera y quarta de la Decada

Obtención del modelo tridimensional de la puerta de La Latina del S. XVI d.C. mediante equipo láser esc��ner terrestre.

El objetivo de este Proyecto Fin de Carrera (PFC) es la obtención de un modelo tridimensional de “La Puerta de la Latina”, ubicada en el aparcamiento delantero de la Escuela Técnica Superior de Arquitectura de la Universidad Politécnica de Madrid. El levantamiento se realiza con el equipo láser esc��ner terrestre Riegl LMS-Z420i. Tras la toma de datos se efectuará el tratamiento de la nube de puntos y se obtendrá una “imagen realista” del modelo, entendiendo por “imagen realista”una representación final en la que, partiendo del modelo digital triangulado, se realiza una asignación de texturas a partir de imágenes obtenidas in situ. Este proyecto pretende ser un ensayo de las posibilidades de esta tecnología en el proceso de la representación tridimensional de edificios de interés arquitectónico e histórico.

Excitation–contraction coupling is unaffected by drastic alteration of the sequence surrounding residues L720–L764 of the α1S II-III loop

The II-III loop of the skeletal muscle dihydropyridine receptor (DHPR) α1S subunit is responsible for bidirectional-signaling interactions with the ryanodine receptor (RyR1): transmitting an orthograde, excitation–contraction (EC) coupling signal to RyR1 and receiving a retrograde, current-enhancing signal from RyR1. Previously, several reports argued for the importance of two distinct regions of the skeletal II-III loop (residues R681–L690 and residues L720–Q765, respectively), claiming for each a key function in DHPR–RyR1 communication. To address whether residues 720–765 of the II-III loop are sufficient to enable skeletal-type (Ca2+ entry-independent) EC coupling and retrograde interaction with RyR1, we constructed a green fluorescent protein (GFP)-tagged chimera (GFP-SkLM) having rabbit skeletal (Sk) DHPR sequence except for a II-III loop (L) from the DHPR of the house fly, Musca domestica (M). The Musca II-III loop (75% dissimilarity to α1S) has no similarity to α1S in the regions R681–L690 and L720–Q765. GFP-SkLM expressed in dysgenic myotubes (which lack endogenous α1S subunits) was unable to restore EC coupling and displayed strongly reduced Ca2+ current densities despite normal surface expression levels and correct triad targeting (colocalization with RyR1). Introducing rabbit α1S residues L720–L764 into the Musca II-III loop of GFP-SkLM (substitution for Musca DHPR residues E724–T755) completely restored bidirectional coupling, indicating its dependence on α1S loop residues 720–764 but its independence from other regions of the loop. Thus, 45 α1S-residues embedded in a very dissimilar background are sufficient to restore bidirectional coupling, indicating that these residues may be a site of a protein–protein interaction required for bidirectional coupling.

RNA polymerase II/III transcription specificity determined by TATA box orientation.

The TATA box sequence in eukaryotes is located about 25 bp upstream of many genes transcribed by RNA polymerase II (Pol II) and some genes transcribed by RNA polymerase III (Pol III). The TATA box is recognized in a sequence-specific manner by the TATA box-binding protein (TBP), an essential factor involved in the initiation of transcription by all three eukaryotic RNA polymerases. We have investigated the recognition of the TATA box by the Pol II and Pol III basal transcription machinery and its role in establishing the RNA polymerase specificity of the promoter. Artificial templates were constructed that contained a canonical TATA box as the sole promoter element but differed in the orientation of the 8-bp TATA box sequence. As expected, Pol II initiated transcription in unfractionated nuclear extracts downstream of the "forward" TATA box. In distinct contrast, transcription that initiated downstream of the "reverse" TATA box was carried out specifically by Pol III. Importantly, this effect was observed regardless of the source of the DNA either upstream or downstream of the TATA sequence. These findings suggest that TBP may bind in opposite orientations on Pol II and Pol III promoters and that opposite, yet homologous, surfaces of TBP may be utilized by the Pol II and Pol III basal machinery for the initiation of transcription.