965 resultados para internal transcribed spacer (ITS)
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Este artículo se enfoca en el estudio del período que corre entre 1935 y 1951 para destacar una de las empresas más importantes del PC a nivel mundial, con substanciales réditos en Argentina: la organización de las mujeres. En particular, nuestro interrogante es de qué modo un partido tradicionalmente considerado de estructuración directa y unitaria, profesional, eminentemente concentrado en la línea de formación ideológica por sobre la electoral, caracterizado por un perfil obrero y fuerte en términos de organización administrativa interna -particularmente, debido a su característica celular-, encuentra límites a esa propia caracterización organizacional en tanto delinea una política de intervención coyuntural que lo coloca en alianzas con otros actores y modifica, con ello, sus propios caracteres. El corpus documental de esta investigación incluye documentos y prensa partidaria (comunista y de otras agrupaciones políticas o sociales); archivos personales, autobiografías y entrevistas. La codificación permitió un ordenamiento de los datos extraídos de los documentos relevados, agrupándolos, según similitudes o diferencias relevantes en categorías que hicieron posible su análisis
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Este artículo se enfoca en el estudio del período que corre entre 1935 y 1951 para destacar una de las empresas más importantes del PC a nivel mundial, con substanciales réditos en Argentina: la organización de las mujeres. En particular, nuestro interrogante es de qué modo un partido tradicionalmente considerado de estructuración directa y unitaria, profesional, eminentemente concentrado en la línea de formación ideológica por sobre la electoral, caracterizado por un perfil obrero y fuerte en términos de organización administrativa interna -particularmente, debido a su característica celular-, encuentra límites a esa propia caracterización organizacional en tanto delinea una política de intervención coyuntural que lo coloca en alianzas con otros actores y modifica, con ello, sus propios caracteres. El corpus documental de esta investigación incluye documentos y prensa partidaria (comunista y de otras agrupaciones políticas o sociales); archivos personales, autobiografías y entrevistas. La codificación permitió un ordenamiento de los datos extraídos de los documentos relevados, agrupándolos, según similitudes o diferencias relevantes en categorías que hicieron posible su análisis
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Hospitals from ancient Seville had an important heritage for survival of the institution and its patients. In order to keep this heritage, the officialdom settled down several control mechanisms that would serve to manage a profitable management of their income and rights. For this purpose, they developed devising instruments able to preserve their possessions and put them into operation. This article attempts to identify the defining elements of these books, called “protocolos de bienes” (protocols goods), indicating their characteristics and evolution from archaic models until the final form. This final form was reached late sixteenth and early seventeenth century, at which time devoted use main codex of hospitality. To do this, we used the documentary collec-tions of Seville, preserved in different archives of the city, from where they have taken several significant examples showing the changes that occurred in both its internal structure and its mate-rials manufacturing, underlining the participation of official, booksellers, illuminators and calligraphers. Similarly, it has high-lighted the multifaceted and multifunctional character of this ins-titutions that became also a corporate identity. The multiplicity of hospitals in Sevilla had different types and features of protocols, which were modificated according to the different needs of each institution.
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Wydział Nauk Politycznych i Dziennikarstwa
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Esta investigación está dirigida a la Administración de Fondos de los diferentes tipos de Asociatividad Municipal: Asociación Municipal, Asociación de Municipios, Asociaciones de Municipalidades y Micro-Regiones de la Zona Paracentral. Los tipos de Asociatividad Municipal a los que se hace referencia en esta investigación administran recursos y obligaciones del sector público, por tanto son sujetos de derechos, atribuciones, y deberes propios, los que en conjunto conforman el ente contable gubernamental. Para el control, organización y registro de las diferentes transacciones de estas instituciones únicamente podrá realizarse en el Sistema de Contabilidad Gubernamental de los Entes (SICGE), aplicación informática proporcionada por el Ministerio de Hacienda. Para conocer el funcionamiento del control interno institucional se realizó un estudio a través de entrevista, encuestas y observación de los procesos, con el objeto de realizar un diagnóstico situacional sobre los procedimientos de controles aplicados. El control interno institucional fomenta la seguridad razonable en las cifras, oportunidad y consistencia en la información financiera, fundamental para la toma de decisiones. Con el objeto de ayudar al fortalecimiento institucional de las mancomunadas se incluye una propuesta de procedimientos de control interno financiero contables, con sus herramientas de control ofimatizadas, diseñados para la administración de los fondos de las Asociaciones de Municipios, que por ser entes contables, es preciso que cada mancomunidad cuente con su propio control interno financiero, que servirá para la transparencia de la gestión y la confiabilidad de la información financiera. EXECUTIVE SUMMARY This research is about at the Fund Management of different types of Municipal Associativity: Municipal Association, Association of Municipalities, Associations of Municipalities and Micro-Regions Paracentral Zone. Municipal Associative types referred to in this investigation about manage resources and obligations of the public sector, so they are subjects of rights, powers, and duties, which together make up the government accounting entity. For control, organization and registration of the several transactions of these institutions may only take place in the Government Accounting System of the Entities (SICGE) for acronym is spanish, computer application provided by the Ministry of Finance. For the functioning of the institutional internal control study was conducted through interviews, surveys and observation of processes in order to conduct a situational analysis on the procedure applied control. The Constitutional internal control promotes reasonable safety in numbers, timeliness and consistency financial information, critical for taking decision. In order to help the institutional strengthening of the asocitiation municipal is included a proposed of procedures of financial-accounting internal control, with its office automatization tools, which is designed for the administration of found the Association of Municipalities (political division of several cities), who is Gubernamental accounting entity, each association must have its own financial internal control, which will serve for management transparency and reliability of financial information.
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Analysis of the structure of the urochordate Herdmania curvata ribosomal DNA intergenic spacer (IGS) and its role in transcription initiation and termination suggests that rRNA gene regulation in this chordate differs from that in vertebrates. A cloned H, curvata IGS is 1881 bp and composed predominantly of two classes of similar repeat sequences that largely alternate in a tandem array. Southern blot hybridization demonstrates that the IGS length variation within an individual and population is largely the result of changes in internal repeat number. Nuclease S1 mapping and primer extension analyses suggest that there are two transcription initiation sites at the 3' end of the most 3' repetitive element; these sites are 6 nucleotides apart. Unlike mouse, Xenopus, and Drosophila, there is no evidence of transcription starting elsewhere in the IGS. Most sequence differences between the promoter repeat and the other internal repeats are in the vicinity of the putative initiation sites. As in Drosophila, nuclease S1 mapping of transcription termination sites suggest that there is not a definitive stop site and a majority of the pre-rRNAs read through a substantial portion of the IGS. Some transcription appears to proceed completely through the promoter repeat into the adjacent rDNA unit. Analysis of oocyte RNA by reverse transcription-polymerase chain reaction (RT-PCR) confirms that readthrough transcription into the adjacent rDNA unit is occurring in some small IGS length variants; there is no evidence of complete readthrough of IGSs larger than 1.0 kb.
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Biomphalaria glabrata can react through different pathways to Schistosoma mansoni miracidium penetration, according to the degree of resistance/susceptibility presented by different snail strains, which is a genetically determined character, resistance being the dominant feature. However, it has been observed that previous susceptible snail strain may change its reactive behavior along the course of infection, exhibiting later a pattern of cercarial shedding and histopatopathological picture compatible with high resistance. Such observation suggests the possibility of B. glabrata to develop a sort of adaptative immunity face a schistosome infection. To explore on this aspect, the present investigation looked for the behavior of S. mansoni infection in B. glabrata previously subjected to different means of artificial stimulation of its internal defense system. Snails previously inoculated with irradiated miracídia (Group I); treated with S. mansoni antigens (Group II) or with a non-related parasite antigen (Group III) were challenged with 20 viable S. mansoni miracidia, and later looked for cercarial shedding and histopathologic changes at different times from exposition. Nodules of hemocyte accumulations were found at the site of antigen injection. These nodules resembled solid granulomas, and were larger and more frequent in snails injected with S. mansoni products as compared to those injected with Capillaria hepatica. However, the presence of such granulomas did not avoid the S. mansoni challenge infection from developing in a similar way as that seen in controls. The data are indicative that hemocytes are able to proliferate locally when stimulated, such capacity also remaining localized, not being shared by the population of hemocytes located elsewhere within the snail body.
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Transcription and translation require a high concentration of potassium across the entire tree of life. The conservation of a high intracellular potassium was an absolute requirement for the evolution of life on Earth. This was achieved by the interplay of P- and V-ATPases that can set up electrochemical gradients across the cell membrane, an energetically costly process requiring the synthesis of ATP by F-ATPases. In animals, the control of an extracellular compartment was achieved by the emergence of multicellular organisms able to produce tight epithelial barriers creating a stable extracellular milieu. Finally, the adaptation to a terrestrian environment was achieved by the evolution of distinct regulatory pathways allowing salt and water conservation. In this review we emphasize the critical and dual role of Na(+)-K(+)-ATPase in the control of the ionic composition of the extracellular fluid and the renin-angiotensin-aldosterone system (RAAS) in salt and water conservation in vertebrates. The action of aldosterone on transepithelial sodium transport by activation of the epithelial sodium channel (ENaC) at the apical membrane and that of Na(+)-K(+)-ATPase at the basolateral membrane may have evolved in lungfish before the emergence of tetrapods. Finally, we discuss the implication of RAAS in the origin of the present pandemia of hypertension and its associated cardiovascular diseases.
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The 23S ribosomal RNA (rRNA) gene has been sequenced in strains of the fish pathogens Photobacterium damselae subsp. damselae (ATCC 33539) and subsp. piscicida (ATCC 29690), showing that 3 nucleotide positions are clearly different between subspecies. In addition, the 5S rRNA gene plus the intergenic spacer region between the 23S and 5S rRNA genes (ITS-2) were amplified, cloned and sequenced for the 2 reference strains as well as the field isolates RG91 (subsp. damselae) and DI21 (subsp. piscicida). A 100% similarity was found for the consensus 5S rRNA gene sequence in the 2 subspecies, although some microheterogeneity was detected as inter-cistronic variability within the same chromosome. Sequence analysis of the spacer region between the 23S and 5S rRNA genes revealed 2 conserved and 3 variable nucleotide sequence blocks, and 4 different modular organizations were found. The ITS-2 spacer region exhibited both inter-subspecies and inter-cistronic polymorphism, with a mosaic-like structure. The EMBL accession numbers for the 23S, 5S and ITS-2 sequences are: P. damselae subsp. piscicida 5S gene (AJ274379), P. damselae subsp. damselae 23S gene (Y18520), subsp. piscicida 23S gene (Y17901), R damselae subsp. piscicida ITS-2 (AJ250695, AJ250696), P. damselae subsp. damselae ITS-2 (AJ250697, AJ250698).
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The length polymorphism of ribosomal DNA ITS-1 intergenic spacer was analyzed in eight species of triatomines belonging to Triatoma, Rhodnius, and Panstrongylus genera. The analyzed species were Rhodnius domesticus, R. neivai, R. robustus, Triatoma brasiliensis, T. infestans, T. vitticeps, Panstrongylus megistus, and P. herreri. These insects are vectors of Chagas' disease, one of the most prominent public health problems among South American countries. This work allowed the differentiation between species of the Triatomini and Rhodniini tribes through the analysis of ITS-1 length polymorphism by PCR and RFLP techniques. The species of the Triatoma and Panstrongylus genera presented an amplified ITS-1 fragment between 600 and 1000 bp, whereas Rhodnius presented a less variable ITS-1 length fragment, around 300 bp, which could reflect the monophyletic origin of the Rhodniini tribe. Species belonging to this genus were further differentiated by RFLP with HaeIII and AluI endonucleases. Our results corroborate the hypothesis of polyphyletic origin in this group of insects and contribute to knowledge about evolutionary relationships in triatomines.
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We analyzed the ITS-1 spacer region of the rDNA in Drosophila mulleri and D. arizonae, two sibling species belonging to the mulleri complex (repleta group) and in hybrids obtained in both cross directions. In spite of several previous studies showing the incompatibility of crosses involving D. arizonae females and D. mulleri males, we were able to obtain hybrids in this direction. Complete ITS-1 region was amplified using primers with homology at the 3'-end of the 18S rDNA and the 5'-end of the 5.8S rDNA genes. Our data demonstrated that D. mulleri and D. arizonae can be differentiated as they present a difference in length for the ITS-1 region. The amplified fragment for this region in D. mulleri has a length of 600 bp, whereas in D. arizonae this fragment is about 500 bp. It was also observed that male and female hybrids obtained in both cross directions present two amplified fragments, confirming the location of the ribosomal cistrons in the X chromosomes and microchromosomes of both parental species.
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BACKGROUND The function of naturally existing internal mammary (IMA)-to-coronary artery bypasses and their quantitative effect on myocardial ischemia are unknown. METHODS AND RESULTS The primary end point of this study was collateral flow index (CFI) obtained during two 1-minute coronary artery balloon occlusions, the first with and the second without simultaneous distal IMA occlusion. The secondary study end point was the quantitatively determined intracoronary ECG ST-segment elevation. CFI is the ratio of simultaneously recorded mean coronary occlusive pressure divided by mean aortic pressure both subtracted by mean central venous pressure. A total of 180 pairs of CFI measurements were performed among 120 patients. With and without IMA occlusion, CFI was 0.110±0.074 and 0.096±0.072, respectively (P<0.0001). The difference of CFI obtained in the presence minus CFI obtained in the absence of IMA occlusion was highest and most consistently positive during left IMA with left anterior descending artery occlusion and during right IMA with right coronary artery occlusion (ipsilateral occlusions): 0.033±0.044 and 0.025±0.027, respectively. This CFI difference was absent during right IMA with left anterior descending artery occlusion and during left IMA with right coronary artery occlusion (contralateral occlusions): -0.007±0.034 and 0.001±0.023, respectively (P=0.0002 versus ipsilateral occlusions). The respective CFI differences during either IMA with left circumflex artery occlusion were inconsistently positive. Intracoronary ECG ST-segment elevations were significantly reduced during ipsilateral IMA occlusions but not during contralateral or left circumflex artery occlusions. CONCLUSION There is a functional, ischemia-reducing extracardiac coronary artery supply via ipsilateral but not via contralateral natural IMA bypasses. CLINICAL TRIAL REGISTRATION URL http://www.clinicaltrials.gov. Unique identifier: NCTO1676207.