499 resultados para destinations
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Coilin is the signature protein of the Cajal body (CB), a nuclear suborganelle involved in the biogenesis of small nuclear ribonucleoproteins (snRNPs). Newly imported Sm-class snRNPs are thought to traffic through CBs before proceeding to their final nuclear destinations. Loss of coilin function in mice leads to significant viability and fertility problems. Coilin interacts directly with the spinal muscular atrophy (SMA) protein via dimethylarginine residues in its C-terminal domain. Although coilin hypomethylation results in delocalization of survival of motor neurons (SMN) from CBs, high concentrations of snRNPs remain within these structures. Thus, CBs appear to be involved in snRNP maturation, but factors that tether snRNPs to CBs have not been described. In this report, we demonstrate that the coilin C-terminal domain binds directly to various Sm and Lsm proteins via their Sm motifs. We show that the region of coilin responsible for this binding activity is separable from that which binds to SMN. Interestingly, U2, U4, U5, and U6 snRNPs interact with the coilin C-terminal domain in a glutathione S-transferase (GST)-pulldown assay, whereas U1 and U7 snRNPs do not. Thus, the ability to interact with free Sm (and Lsm) proteins as well as with intact snRNPs, indicates that coilin and CBs may facilitate the modification of newly formed snRNPs, the regeneration of 'mature' snRNPs, or the reclamation of unassembled snRNP components.
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BACKGROUND International travel contributes to the worldwide spread of multidrug resistant Gram-negative bacteria. Rates of travel-related faecal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae vary for different destinations. Especially travellers returning from the Indian subcontinent show high colonization rates. So far, nothing is known about region-specific risk factors for becoming colonized. METHODS An observational prospective multicentre cohort study investigated travellers to South Asia. Before and after travelling, rectal swabs were screened for third-generation cephalosporin- and carbapenem-resistant Enterobacteriaceae. Participants completed questionnaires to identify risk factors for becoming colonized. Covariates were assessed univariately, followed by a multivariate regression. RESULTS Hundred and seventy persons were enrolled, the largest data set on travellers to the Indian subcontinent so far. The acquired colonization rate with ESBL-producing Escherichia coli overall was 69.4% (95% CI 62.1-75.9%), being highest in travellers returning from India (86.8%; 95% CI 78.5-95.0%) and lowest in travellers returning from Sri Lanka (34.7%; 95% CI 22.9-48.7%). Associated risk factors were travel destination, length of stay, visiting friends and relatives, and eating ice cream and pastry. CONCLUSIONS High colonization rates with ESBL-producing Enterobacteriaceae were found in travellers returning from South Asia. Though risk factors were identified, a more common source, i.e. environmental, appears to better explain the high colonization rates.
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AIM OF PAPER AND RESEARCH QUESTIONS The primary aim of this investigation was to examine, if tourism in Switzerland can be stimulated by focusing on e-bike offers. Switzerland is an attractive and manifold country for bike tourism. However, there are plenty of hilly and steep areas, where the topography is quite demanding and cycling is predestined to ambitious sportsperson. Less performance-oriented bike tourists are possibly discouraged by the challenges of the landscape. E-bikes seem to be a reasonable alterna-tive to enable less trained person to attend bike tours in steeper regions as well as to keep heterogeneous groups together. E-bikes are already popular in Switzerland for daily routes as the journey to work, but they are not very common in the tour-istic context. The presented investigation evaluates the current standing of supply and demand for e-bike tourism and asks for opportunities and threats in the future development. LITERTURE REVIEW The existing frameworks to analyse capability in the field of sport tourism are limited, because of the broad variation of sports and tourism types. Still several conceptions are valuable to evaluate the opportunities of e-bike tourism in Switzer-land. According to Higham and Hinch (2009) the potential of touristic products and destinations always depends on the interaction among the factors place, people and activity. Standeven and de Knop (1999) support this position by identify-ing the experience of place as a key component of the sport tourism experience. Bull (2005) assumes that place not only affects experience. In his opinion, the specific spatially located resources are even crucial for the existence of nature sports. He identifies four factors determining the attraction of touristic products or destination, as physical characteristics, accessibility and infrastructural arrangements, political and economic resources as well as cultural and perceptual aspects. Concerning the demand side (people) the contemporary research is guided by an individual psychological focus delivering mainly results about motives and the decision making process (e.g. Görtz & Hürten, 2011). RESEARCH DESIGN AND DATA ANALYSIS The presented study is based on a between method triangulation, consisting of qualitative interviews with important stake-holders on the supply side and a cross sectional survey on the demand side. Qualitative interviews were conducted with the first provider of touristic e-bike routes and with the manager of the leading e-bike rental company in Switzerland. The interviews were evaluated by qualitative content analysis according to Mayring (2008). The survey covered a randomized sample of 748 adult persons and focused on interests and needs of potential consumers. The concluding potentials analy-sis combined the results of the interviews and the survey with the findings of literature research. As central outcome the strengths, weaknesses, opportunities and threats were evaluated and presented by SWOT-analysis. RESULTS Results showed that the development of e-bike tourism in Switzerland was not initiated by tourism promoter, but by an innovative e-bike producer. However, also for the manufacturer the fit between landscape (place), product (activity) and visitors (people) was the crucial criterion. The first e-bike tours were offered in a demanding and rural region, in order that the experience of place was able to promote a positive overall experience. Due to the success of the first touristic e-bike products, several tourism regions started to extend their portfolio with e-bike offers. To date a nationwide network for signalised e-bike tours, rental stations and changing stations for rechargeable batteries is established. Despite the attractive offer, the demand is only moderate. The obtained results of the consumer survey pointed at a certain barrier to use e-bikes for tourism activities. The most substantial barrier is the missing affinity for bike tourism in general. Another notable group considering themselves as “fit enough for normal bike tourism”. Nevertheless 55% of the respondent are interested in tour-istic e-bike products. Looking only at people with e-bike tourism experience, even 92% are interested in further activities. DISCUSSION AN CONCLUSION The current study findings are encouraging because they suggest a superior suitability and an attractive level of e-bike tourism products in Switzerland. The results of the consumer survey indicate an increasing demand for e-bike tourism. The investigation also points at some risks, as the rivalry for bike tourism or the raised safety hazard because of the increased driving speed. Summing up, the results support the conclusion, that e-bike tourism will become more important in the com-ing years. However, to reach the goal relevant trends as the requirement for customised offers must be considered and marketing activities are supposed to be extended. REFERENCES Bull, C. (2005). Sport tourism resource analysis. In J. Higham (Ed.), Sport tourism destination: Issues, opportunities and analysis (pp. 25-38). Amsterdam: Elsevier Butterworth-Heinemann. Görtz, M., & D. Hürten (2011). Motive der Radurlauber, psychografische Merkmale und Reiseverhalten. In A. Dreyer, E. Miglbauer & R. Mühlnickel (Hrsg.), Radtourismus. Entwicklungen, Potenziale, Perspektiven (S. 36-43). München: Olden-bourg. Higham, J., & Hinch, T. (2009). Sport and Tourism. Globalization, Mobility and Identity. Amsterdam: Butterworth-Heinemann. Mayring, P. (2008). Qualitative Inhaltsanalyse. Grundlagen und Techniken (10. Aufl.). Weinheim: Beltz Verlag. Standeven, J., & De Knop, P. (1999). Sport Tourism. Campaign: Human Kinetics.
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OBJECTIVE To assess whether exposure to high altitude induces cognitive dysfunction in young healthy European children and adolescents during acute, short-term exposure to an altitude of 3450 m and in an age-matched European population permanently living at this altitude. STUDY DESIGN We tested executive function (inhibition, shifting, and working memory), memory (verbal, short-term visuospatial, and verbal episodic memory), and speed processing ability in: (1) 48 healthy nonacclimatized European children and adolescents, 24 hours after arrival at high altitude and 3 months after return to low altitude; (2) 21 matched European subjects permanently living at high altitude; and (3) a matched control group tested twice at low altitude. RESULTS Short-term hypoxia significantly impaired all but 2 (visuospatial memory and processing speed) of the neuropsychological abilities that were tested. These impairments were even more severe in the children permanently living at high altitude. Three months after return to low altitude, the neuropsychological performances significantly improved and were comparable with those observed in the control group tested only at low altitude. CONCLUSIONS Acute short-term exposure to an altitude at which major tourist destinations are located induces marked executive and memory deficits in healthy children. These deficits are equally marked or more severe in children permanently living at high altitude and are expected to impair their learning abilities.
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Glycerophospholipids are the principal fabric of cellular membranes. The pathways by which these lipids are synthesized were elucidated mainly through the work of Kennedy and colleagues in the late 1950s and early 1960s. Subsequently, attention turned to cell biological aspects of lipids: Where in the cell are lipids synthesized? How are lipids integrated into membranes to form a bilayer? How are they sorted and transported from their site of synthesis to other cellular destinations? These topics, collectively termed 'lipid topogenesis', were the subject of a review article in 1981 by Bell, Ballas and Coleman. We now assess what has been learned about early events of lipid topogenesis, i.e. "lipid synthesis, the integration of lipids into membranes, and lipid translocation across membranes", in the 35years since the publication of this important review. We highlight the recent elucidation of the X-ray structures of key membrane enzymes of glycerophospholipid synthesis, progress on identifying lipid scramblase proteins needed to equilibrate lipids across membranes, and new complexities in the subcellular location and membrane topology of phosphatidylinositol synthesis revealed through a comparison of two unicellular model eukaryotes.
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Background. Large field studies in travelers' diarrhea (TD) in multiple destinations are limited by the need to perform stool cultures on site in a timely manner. A method for the collection, transport and storage of fecal specimens that does not require immediate processing, refrigeration and is stable for months would be advantageous. ^ Objectives. Determine if enteric pathogen bacterial DNA can be identified in cards routinely used for evaluation of fecal occult blood. ^ Methods. U.S. students traveling to Mexico in 2005-07 were followed for occurrence of diarrheal illness. When ill, students provided a stool specimen for culture and occult blood by the standard method. Cards were then stored at room temperature prior to DNA extraction. A multiplex fecal PCR was performed to identify enterotoxigenic Escherichia coli and enteroaggregative E. coli (EAEC) in DNA extracted from stools and occult blood cards. ^ Results. Significantly more EAEC cases were identified by PCR done in DNA extracted from cards (49%) or from frozen feces (40%) than by culture followed by HEp-2 adherence assays (13%). Similarly more ETEC cases were detected in card DNA (38%) than fecal DNA (30%) or culture followed by hybridization (10%). Sensitivity and specificity of the card test was 75% and 62%, respectively, and 50% and 63%, respectively, when compared to EAEC and ETEC culture, respectively, and 53% and 51%, respectively compared to EAEC multiplex fecal PCR and 56% and 70%, respectively, compared to ETEC multiplex fecal PCR. ^ Conclusions. DNA extracted from fecal cards used for detection of occult blood is of use in detecting enteric pathogens. ^
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En este artículo se analizan los cambios que se están experimentando en el comercio mundial de la pera. Su objetivo es conocer si los procesos de integración entre países están repercutiendo en el comercio internacional de peras y por lo tanto afectando su competitividad. Como metodología se emplea el Índice de Competitividad del Comercio Exterior (ICCE) que analiza la competitividad en un país para un producto en particular con nomenclador arancelario, abarcando sus principales destinos en las exportaciones y observando la evolución y tendencia de la cuota de participación en los principales mercados, y de esa forma evaluar a los competidores. Como resultado se observa cómo la competitividad del comercio mundial de la pera no es global y sí regional, y los países, en general, tienen sus mercados relevantes en territorios cercanos o con acuerdos comerciales. Ante este panorama es necesario la adaptación hacia un nuevo paradigma de mercado.
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The article discusses the memories and the history stored in the family archive of Francis Mouro, migrant in Rio de Janeiro, in the first half of the twentieth century. The Mouro family, since the nineteenth century, decided to adopt Brazil as preferred destination. However, like so many Galician families also choose other destinations, moving to Portugal, Uruguay and Argentina. Through letters and personal documents preserved by the immigrant family is possible to reconstruct the migratory chains formed over more than half a century, the solidarity networks and migration of a city of intense immigration to Rio de Janeiro, as the galego city Santa Comba
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Hasta principios del siglo XX la región de Castilla y León no figuraba en el cómputo de las regiones migratorias, situadas básicamente en la periferia mediterránea o en el noroeste español. En este texto se examinan brevemente las causas de este débil pulso migratorio como las que provocaron luego el éxodo masivo a América. Se exponen luego muy sumariamente las políticas migratorias de Argentina, Cuba y Brasil, principales países a los que se dirigían los castellanos. Teniendo en cuenta la decisiva importancia que la emigración española desempeñó en la formación del mercado de trabajo, podremos conocer mejor la evolución del fenómeno migratorio si examinamos cuándo y por qué se plantearon las distintas políticas de inmigración. Finalmente se analiza cómo se concretaron esas políticas de inmigración para conocer los grados de preferencia por un tipo u otro de emigrantes.
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Este artículo se propone interrogar las relaciones entre comunidad diaspórica catalana del Cono Sur y prácticas regionales solidarias, enfatizando aquellos proyectos e iniciativas que se articularon a ambos lados de la cordillera es pos de la ayuda hacia los desplazados (primero los desplazados al interior de la península durante la Guerra Civil, luego los desplazados a Francia tras la "retirada" y por último los evacuados desde el país galo hacia América Latina). Asimismo, intenta pensar desde un conjunto de trayectorias individuales y grupales de destierro que tuvieron como destinos a Chile y la Argentina, en qué medida estos países de recepción operaron, para esos sujetos y también para las instituciones (oficiales o societales catalanas) generadoras del auxilio, como un espacio poroso de tránsitos, de relaciones fluidas, de intercambio de información y de proyectos comunes, cimentado en complejas redes familiares, político-partidarias, profesionales e ideológicas, transfronterizas y también transnacionales
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Este artículo se propone interrogar las relaciones entre comunidad diaspórica catalana del Cono Sur y prácticas regionales solidarias, enfatizando aquellos proyectos e iniciativas que se articularon a ambos lados de la cordillera es pos de la ayuda hacia los desplazados (primero los desplazados al interior de la península durante la Guerra Civil, luego los desplazados a Francia tras la "retirada" y por último los evacuados desde el país galo hacia América Latina). Asimismo, intenta pensar desde un conjunto de trayectorias individuales y grupales de destierro que tuvieron como destinos a Chile y la Argentina, en qué medida estos países de recepción operaron, para esos sujetos y también para las instituciones (oficiales o societales catalanas) generadoras del auxilio, como un espacio poroso de tránsitos, de relaciones fluidas, de intercambio de información y de proyectos comunes, cimentado en complejas redes familiares, político-partidarias, profesionales e ideológicas, transfronterizas y también transnacionales
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The article discusses the memories and the history stored in the family archive of Francis Mouro, migrant in Rio de Janeiro, in the first half of the twentieth century. The Mouro family, since the nineteenth century, decided to adopt Brazil as preferred destination. However, like so many Galician families also choose other destinations, moving to Portugal, Uruguay and Argentina. Through letters and personal documents preserved by the immigrant family is possible to reconstruct the migratory chains formed over more than half a century, the solidarity networks and migration of a city of intense immigration to Rio de Janeiro, as the galego city Santa Comba
Resumo:
Hasta principios del siglo XX la región de Castilla y León no figuraba en el cómputo de las regiones migratorias, situadas básicamente en la periferia mediterránea o en el noroeste español. En este texto se examinan brevemente las causas de este débil pulso migratorio como las que provocaron luego el éxodo masivo a América. Se exponen luego muy sumariamente las políticas migratorias de Argentina, Cuba y Brasil, principales países a los que se dirigían los castellanos. Teniendo en cuenta la decisiva importancia que la emigración española desempeñó en la formación del mercado de trabajo, podremos conocer mejor la evolución del fenómeno migratorio si examinamos cuándo y por qué se plantearon las distintas políticas de inmigración. Finalmente se analiza cómo se concretaron esas políticas de inmigración para conocer los grados de preferencia por un tipo u otro de emigrantes.
Resumo:
Este artículo se propone interrogar las relaciones entre comunidad diaspórica catalana del Cono Sur y prácticas regionales solidarias, enfatizando aquellos proyectos e iniciativas que se articularon a ambos lados de la cordillera es pos de la ayuda hacia los desplazados (primero los desplazados al interior de la península durante la Guerra Civil, luego los desplazados a Francia tras la "retirada" y por último los evacuados desde el país galo hacia América Latina). Asimismo, intenta pensar desde un conjunto de trayectorias individuales y grupales de destierro que tuvieron como destinos a Chile y la Argentina, en qué medida estos países de recepción operaron, para esos sujetos y también para las instituciones (oficiales o societales catalanas) generadoras del auxilio, como un espacio poroso de tránsitos, de relaciones fluidas, de intercambio de información y de proyectos comunes, cimentado en complejas redes familiares, político-partidarias, profesionales e ideológicas, transfronterizas y también transnacionales
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The article discusses the memories and the history stored in the family archive of Francis Mouro, migrant in Rio de Janeiro, in the first half of the twentieth century. The Mouro family, since the nineteenth century, decided to adopt Brazil as preferred destination. However, like so many Galician families also choose other destinations, moving to Portugal, Uruguay and Argentina. Through letters and personal documents preserved by the immigrant family is possible to reconstruct the migratory chains formed over more than half a century, the solidarity networks and migration of a city of intense immigration to Rio de Janeiro, as the galego city Santa Comba