Phytoplankton composition and biomass and its fluorescence parameters in waters of the Nha Trang Bay, South China Sea

Species composition and abundance of phytoplankton and chlorophyll concentration were measured at three horizons of 9 stations in the Nha Trang Bay of the South China Sea in March 1998. Vertical distribution of fluorescence parameters, temperature and irradiance were measured in the 0-18 m layer of the water column at 21 stations. It was shown that according to biomass (B) and chlorophyll concentration (Chl) the Bay is mezotrophic. B and Chl in the water column increased seaward. Mean values of Chl in the southern part of the Bay exceeded those in northern part. Mean values of B were similar. B and Chl in the bottom layer exceeded ones in the upper layer. Diatoms dominated in species diversity and abundance. Diatom Guinardia striata made the main contribution to phytoplankton biomass. Similarity of phytoplankton was high. In the upper layer phytoplankton was photoinhibited during the most part of the light period, but at the bottom photosynthetic activity was high. Water column B varied in an order of magnitude during the daily cycle mainly because of B variations in the bottom layer due to tide flow.

Ocean acidification outweighs nutrient effects in structuring seagrass epiphyte communities

1. Developing a framework for assessing interactions between multiple anthropogenic stressors remains an important goal in environmental research. In coastal ecosystems, the relative effects of aspects of global climate change (e.g. CO2 concentrations) and localized stressors (e.g. eutrophication), in combination, have received limited attention. 2. Using a long-term (11 month) field experiment, we examine how epiphyte assemblages in a tropical seagrass meadow respond to factorial manipulations of dissolved carbon dioxide (CO2(aq)) and nutrient enrichment. In situ CO2(aq) manipulations were conducted using clear, open-top chambers, which replicated carbonate parameter forecasts for the year 2100. Nutrient enrichment consisted of monthly additions of slow-release fertilizer, nitrogen (N) and phosphorus (P), to the sediments at rates equivalent to theoretical maximum rates of anthropogenic loading within the region (1.54 g N/m**2/d and 0.24 g P m**2/d). 3. Epiphyte community structure was assessed on a seasonal basis and revealed declines in the abundance of coralline algae, along with increases in filamentous algae under elevated CO2(aq). Surprisingly, nutrient enrichment had no effect on epiphyte community structure or overall epiphyte loading. Interactions between CO2(aq) and nutrient enrichment were not detected. Furthermore, CO2(aq)-mediated responses in the epiphyte community displayed strong seasonality, suggesting that climate change studies in variable environments should be conducted over extended time-scales. 4. Synthesis. The observed responses indicate that for certain locations, global stressors such as ocean acidification may take precedence over local eutrophication in altering the community structure of seagrass epiphyte assemblages. Given that nutrient-driven algal overgrowth is commonly cited as a widespread cause of seagrass decline, our findings highlight that alternate climate change forces may exert proximate control over epiphyte community structure.

Nitrogen fuelling of the pelagic food web of the Tropical Atlantic

We estimated the relative contribution of atmospheric Nitrogen (N) input (wet and dry deposition and N fixation) to the epipelagic food web by measuring N isotopes of different functional groups of epipelagic zooplankton along 23°W (17°N-4°S) and 18°N (20-24°W) in the Eastern Tropical Atlantic. Results were related to water column observations of nutrient distribution and vertical diffusive flux as well as colony abundance of Trichodesmium obtained with an Underwater Vision Profiler (UVP5). The thickness and depth of the nitracline and phosphocline proved to be significant predictors of zooplankton stable N isotope values. Atmospheric N input was highest (61% of total N) in the strongly stratified and oligotrophic region between 3 and 7°N, which featured very high depth-integrated Trichodesmium abundance (up to 9.4×104 colonies m-2), strong thermohaline stratification and low zooplankton delta15N (~2 per mil). Relative atmospheric N input was lowest south of the equatorial upwelling between 3 and 5°S (27%). Values in the Guinea Dome region and north of Cape Verde ranged between 45 and 50%, respectively. The microstructure-derived estimate of the vertical diffusive N flux in the equatorial region was about one order of magnitude higher than in any other area (approximately 8 mmol m-2 d 1). At the same time, this region received considerable atmospheric N input (35% of total). In general, zooplankton delta15N and Trichodesmium abundance were closely correlated, indicating that N fixation is the major source of atmospheric N input. Although Trichodesmium is not the only N fixing organism, its abundance can be used with high confidence to estimate the relative atmospheric N input in the tropical Atlantic (r2 = 0.95). Estimates of absolute N fixation rates are two- to tenfold higher than incubation-derived rates reported for the same regions. Our approach integrates over large spatial and temporal scales and also quantifies fixed N released as dissolved inorganic and organic N. In a global analysis, it may thus help to close the gap in oceanic N budgets.

Cytotoxic analogs of luteinizing hormone-releasing hormone containing doxorubicin or 2-pyrrolinodoxorubicin, a derivative 500-1000 times more potent.

Doxorubicin (DOX) and its daunosamine-modified derivative, 2-pyrrolino-DOX, which is 500-1000 times more active than DOX, were incorporated into agonistic and antagonistic analogs of luteinizing hormone-releasing hormone (LH-RH). The conjugation of DOX with LH-RH analogs was performed by using N-(9-fluorenylmethoxycarbonyl)-DOX-14-O-hemiglutarate, a dicarboxylic acid ester derivative of DOX. Coupling this derivative covalently to the epsilon-amino group of the D-Lys side chain of agonist [D-Lys6]LH-RH or antagonistic analog AC-D-Nal(2)-D-Phe(4Cl)-D-Pal(3)-Ser-Tyr-D-Lys-Leu-Arg-Pro-D-Ala-NH 2 [where Nal(2) = 3-(2-naphthyl)alanine, Pal(3) = 3-(3-pyridyl)alanine, and Phe(4CI) = 4-chlorophenylalanine] was followed by the removal of the 9-fluorenylmethoxycarbonyl protective group to yield cytotoxic derivatives of LH-RH analogs containing DOX. From these DOX containing LH-RH hybrids, intensely potent analogs with daunosamine-modified derivatives of DOX can be readily formed. Thus, cytotoxic LH-RH agonist containing DOX (AN-152) can be converted in a 66% yield by a reaction with a 30-fold excess of 4-iodobutyraldehyde in N,N-dimethylformamide into a derivative having 2-pyrrolino-DOX (AN-207). Hybrid molecules AN-152 and AN-207 fully preserve the cytotoxic activity of their radicals, DOX or 2-pyrrolino-DOX, respectively, in vitro, and also retain the high binding affinity of the peptide hormone portion of the conjugates to rat pituitary receptors for LH-RH. These highly potent cytotoxic analogs of LH-RH were designed as targeted anti-cancer agents for the treatment of various tumors that possess receptors for the carrier peptide. Initial in vivo studies show that the hybrid molecules are much less toxic than the respective cytotoxic radicals incorporated and significantly more active in inhibiting tumor growth.

Majmūʻat al-rasāʼil fī ādāb al-baḥth : manuscript, 1869 or 70

1. Risālah fī ādāb al-muṭālaʻah / Ḥāmid ibn Burhān ibn Abī Dharr al-Ghifārī (ff. 1r-6v) -- 2. Ādāb ʻUthmānīyah (ff. 7r-14r) -- 3. Ādāb Sharīfīyah fī al-munāẓarah / al-Sayyid al-Sharīf al-Jurjānī (ff. 15r-27r) -- 4. Sharḥ ādāb al-baḥth fī ʻilm al-munāẓarah lil-ʻAḍudī (ff. 28r-46v) -- 5. Ḥāshiyah ʻalá Sharḥ Ādāb al-Ḥanafī (ff. 47r-55v).

Lithogenic and biogenic daily and annual particle fluxes on the Lomonosov Ridge of trap LOMO-2

Investigations of lithogenic and biogenic particle fluxes using long-term sediment traps are still very rare in the northern high latitudes and restricted to the arctic marginal seas and sub-arctic regions. Here, for the first time, data on the variability of fluxes of lithogenic matter, carbonate, opal, and organic carbon as well as biomarker composition from the central Arctic Ocean are presented for a one-year period. The study has been carried out on material obtained from a long-term mooring system equipped with two multi-sampling-traps (150 and 1550 m water depth) and deployed on the southern Lomonosov Ridge close to the Laptev Sea continental margin from September 1995 to August 1996. In addition, data from surface-sediments were included in the study to get more information about the flux and sedimentation of organic carbon in this area. Annual fluxes of lithogenic matter, carbonate, opal, and particulate organic carbon are 3.9 g/m**2/y, 0.8 g/m**2/y, 2.6 g/m**2/y, 1.5 g/m**2/y, respectively, at the shallow trap and 11.3 g/m**2/y, 0.5 g/m**2/y, 2.9 g/m**2/y, 1.05 g/m**2/y, respectively, at the deep trap. Both the shallow as well as the deep trap show significant differences in vertical flux values over the year. Higher values were found from mid-July to end of October (total flux of 75-130 mg/m**2/d in the shallow trap and 40-225 mg/m**2/d in the deep trap, respectively). During all other months, fluxes were fairly low in both traps (most total flux values <10 mg/m**2/d1). The interval of increased fluxes can be separated into (1) a mid-July/August maximum caused by increased primary production as documented in high abundances of marine biomarkers and diatoms, and (2) a September/October (absolute) maximum caused by increased influence of Lena river discharge indicated by maximum lithogenic flux and high portions of terrigenous/fluvial biomarkers in both traps. Here, total fluxes in the deep trap were significantly higher than in the shallow trap, suggesting a lateral sediment flux at greater depth. The lithogenic flux data also support the importance of sediment input from the Laptev Sea for the sediment accumulation on the Lomonosov Ridge on geological time scales, as indicated in sedimentary records from this region.

Net community production, Thorium-234 activity and particle flux during the Southern Ocean iron fertilization experiment LOHAFEX

A closed eddy core in the Subantarctic Atlantic Ocean was fertilized twice with two tons of iron (as FeSO4), and the 300 km**2 fertilized patch was studied for 39 days to test whether fertilization enhances downward particle flux into the deep ocean. Chlorophyll a and primary productivity doubled after fertilization, and photosynthetic quantum yield (FV/FM) increased from 0.33 to >0.40. Silicic acid (<2 µmol/L) limited diatoms, which contributed <10% of phytoplankton biomass. Copepods exerted high grazing pressure. This is the first study of particle flux out of an artificially fertilized bloom with very low diatom biomass. Net community production (NCP) inside the patch, estimated from O2:Ar ratios, averaged 21 mmol POC/m**2/d, probably ±20%. 234Th profiles implied constant export of ~6.3 mmol POC/m**2/d in the patch, similar to unfertilized waters. The difference between NCP and 234Th-derived export partly accumulated in the mixed layer and was partly remineralized between the mixed layer and 100 m. Neutrally buoyant sediment traps at 200 and 450 m inside and outside the patch caught mostly <1.1 mmol POC/m**2/d, predominantly of fecal origin; flux did not increase upon fertilization. Our data thus indicate intense flux attenuation between 100 and 200 m, and probably between the mixed layer and 100 m. We attribute the lack of fertilization-induced export to silicon limitation of diatoms and reprocessing of sinking particles by detritus feeders. Our data are consistent with the view that nitrate-rich but silicate-deficient waters are not poised for enhanced particle export upon iron addition.

œuvres de Mr. Turgot, ministre d'état, précédées et accompagnées de Mémoires et de notes sur sa vie, son administration et ses ouvrages ...

Vol. 1: Mémoires sur la vie, l'administration et les ouvrages de M. Turgot, by the editor, Dupont de Nemours.

Répertoire des livres d'occasion en vente à la librairie Lucien Dorbon ...

Half title, binder's title: Repertoire de la Librairie Lucien Dorbon.

No 1, 2, 3 of Haydn's grand symphonies : composed for Mr Salomon's concerts /

1. C major (M.97) -- 2. D major (M.93) -- 3. G major (M.94)

Oeuvres complètes : mises en ordre, revues et annotées d'après les manuscrits de l'auteur.

t.1. 2. éd., 1862.--t.2. 2. éd., 1862.--t.3. 3. éd., 1864.--t.4. 2. éd., 1863.--t.5. 2. éd., 1863.--t.6. 5. éd., 1864.--t.7. 1864.

Catalogue des factums et d'autres documents judiciaires anterieurs a 1790 /

Vol. 10 has imprint: Paris, Editions des Bibliotheques nationales.

Mémoires d'une contemporaine; ou, Souvenirs d'une femme sur les principaux personnages de la république, du consulat, de l'empire, etc. ...

"Lettres inédites de Napoléon Bonaparte, général en chef de l'armée d'Italie": v. 2, p. [347]-395.

The efficacy of a vitamin D-3 metabolite for improving the myofibrillar tenderness of meat from Bos indicus cattle

The influence of a once only administration of a metabolite of vitamin D-3 (HY center dot D-(R)-25-hydroxy vitamin D-3) on myofibrillar meat tenderness in Australian Brahman cattle was studied. Ninety-six Brahman steers of three phenotypes (indo-Brazil, US and US/European) and with two previous hormonal growth promotant (HGP) histories (implanted or not implanted with Compudose((R))) were fed a standard feedlot ration for 70 d. Treatment groups of 24 steers were offered daily 10 g/head HY center dot D-(R) (125 mg 25-hydroxyvitamin D-3) for 6, 4, or 2 d before slaughter. One other group of 24 steers was given the basal diet without HY center dot D-(R). Feed lot performance, blood and muscle samples and carcass quality data were collected at slaughter. Calcium, magnesium, potassium, sodium, iron and Vitamin D-3 metabolites were measured in plasma and longissimus dorsi muscle. Warner-Bratzler (WB) shear force (peak force, initial yield) and other objective meat quality measurements were made on the longissimus dorsi muscle of each steer after ageing for 1, 7 and 14 d post-mortem at 0-2 degrees C. There were no significant effects of HY center dot D-(R) supplements on average daily gain (ADG, 1.28-1.45 kg/d) over the experimental period. HY center dot D-(R) supplements given 6 d prior to slaughter resulted in significantly higher (P < 0.05) initial yield values compared to supplements given 2 d prior to slaughter. Supplementation had no significant effect on meat colour, ultimate pH, sarcomere length, cooking loss, instron compression or peak force. There was a significant treatment (HY center dot D-(R)) by phenotype/HGP interaction for peak force (P = 0.028), in which Indo-Brazil steers without previous HGP treatment responded positively (increased tenderness) to HY center dot D-(R) supplements at 2 d when compared with Indo-Brazil steers previously given HGP. There were no significant effects of treatment on other phenotypes. HY center dot D-(R) supplements did not affect muscle or plasma concentrations of calcium, potassium or sodium, but did significantly decrease plasma magnesium and iron concentrations when given 2 d before slaughter. There were no detectable amounts of 25-hydroxyvitamin D-3 in the blood or muscle of any cattle at slaughter. (c) 2005 Elsevier Ltd. All rights reserved.