[Pyramide de canons pris à l'ennemi au rond-point des Champs-Elysées, autour du 13 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54858

Delbord, Championnat de Paris, plongeon [Choisy-le-Roi, le 27 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55188

Championnat de Paris [de natation], Pouilley gagnant [du] 100 m libre [Choisy-le-Roi, le 27 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55187

Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples

In this study, we evaluated several techniques for the detection of the yeast form of Cryptococcus in decaying wood and measured the viability of these fungi in environmental samples stored in the laboratory. Samples were collected from a tree known to be positive for Cryptococcus and were each inoculated on 10 Niger seed agar (NSA) plates. The conventional technique (CT) yielded a greater number of positive samples and indicated a higher fungal density [in colony forming units per gram of wood (CFU.g-1)] compared to the humid swab technique (ST). However, the difference in positive and false negative results between the CT-ST was not significant. The threshold of detection for the CT was 0.05.10³ CFU.g-1, while the threshold for the ST was greater than 0.1.10³ CFU-1. No colonies were recovered using the dry swab technique. We also determined the viability of Cryptococcus in wood samples stored for 45 days at 25ºC using the CT and ST and found that samples not only continued to yield a positive response, but also exhibited an increase in CFU.g-1, suggesting that Cryptococcus is able to grow in stored environmental samples. The ST.1, in which samples collected with swabs were immediately plated on NSA medium, was more efficient and less laborious than either the CT or ST and required approximately 10 min to perform; however, additional studies are needed to validate this technique.

Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon).

BACKGROUND Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. OBJECTIVE To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma MATERIALS AND METHODS An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. RESULTS Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases. CONCLUSIONS Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens.

M. Katz [chef du service français à l'ambassade d'Espagne à Bruxelles en 1917, cité dans le procès Marguliès en août 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55330

[Rue du Faubourg Poissonnière avec une banderole en l'honneur des poilus, 13 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54883

[Rue Edouard VII à Paris, décorée pour les fêtes de la Victoire, 13 juillet 1919, avec le théâtre Edouard VII au fond] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54880

[Trophée offert pour la traversée de Paris à la nage 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55211

Traversée de Paris à la nage, [Luigi] Bacigalupo (2e) [Italien, 20 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55071

Mariage du prince Axel de Danemark avec la princesse Margareth[a] de Suède [à Stockholm le 22 mai 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54155

[Foule importante place de la Concorde, obélisque enguirlandé, autour du 13 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54847

Delabre sur Rover, 750 cmc [course motocycliste Paris-Reims-Paris, 6 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54747

Lecomte sur Rover, 500 cmc [course motocycliste Paris-Reims-Paris, side-car, 6 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54746

Barthélémy sur Rover, 500 cmc [course motocycliste Paris-Reims-Paris, 6 juillet 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54744