Molecular and isotopic characterization of biomarkers in the Frick Swiss Jura sediments: A palaeoenvironmental reconstruction on the northern Tethys margin

Molecular and stable carbon isotope compositions of source-specific hydrocarbons have been used to reconstruct palaeoenvironmental conditions during deposition of the Middle Hettangian to Upper Sinemurian sediments on the northern epicontinental Tethys margin, Frick Swiss Jura. Increasing algal, cyanobacterial and phytoplanktonic (i.e., dinoflagellate) contributions associated with the C-13-enrichment of cyanobacteria derivatives (i.e., hopanes and monomethylalkanes) suggest enhanced primary productivity upsection. This is related to the C-13-enrichment of dissolved CO2 in the upper layers and the progressive increase of depth and oxygenation of the water column. In the Middle Hettangian shallow-water environments (lagoon), the occurrence of green sulfur bacteria (Chlorobiaceae) derivatives indicates that the lower part of the water column was strictly anoxic and rich in H2S. Since these bacteria require very low light intensity to grow, these euxinic conditions may be extended up to the photic zone, allowing for anaerobic photosynthesis. Light penetration depth is most likely reduced by high productivity and/or turbidity in the photic zone. In these sediments, C-13-depleted hopanoids (-39.5 parts per thousand) are most likely associated with phototrophic purple sulfur bacteria utilizing isotopically light organic carbon at the base of the aerobic zone. These purple sulfur bacteria may have consumed the H2S used by Chlorobiaceae in the deeper layers and thus, sustained the algae and cyanobacteria productivity in the upper layers. The C-13-depleted carbonate (-13.3 parts per thousand) may be partially related to the anaerobic oxidation of the organic matter during bacterial sulfate-reduction. (c) 2006 Elsevier Ltd. All rights reserved.

A flow cytometry based oligotrophic pollutant exposure test to detect bacterial growth inhibition and cell injury.

Toxicity of chemical pollutants in aquatic environments is often addressed by assays that inquire reproductive inhibition of test microorganisms, such as algae or bacteria. Those tests, however, assess growth of populations as a whole via macroscopic methods such as culture turbidity or colony-forming units. Here we use flow cytometry to interrogate the fate of individual cells in low-density populations of the bacterium Pseudomonas fluorescens SV3 exposed or not under oligotrophic conditions to a number of common pollutants, some of which derive from oil contamination. Cells were stained at regular time intervals during the exposure assay with fluorescent dyes that detect membrane injury (i.e., live-dead assay). Reduction of population growth rates was observed upon toxicant insult and depended on the type of toxicant. Modeling and cell staining indicate that population growth rate decrease is a combined effect of an increased number of injured cells that may or may not multiply, and live cells dividing at normal growth rates. The oligotrophic assay concept presented here could be a useful complement for existing biomarker assays in compliance with new regulations on chemical effect studies or, more specifically, for judging recovery after exposure to fluctuating toxicant conditions.

Classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of Chaco, Argentina

To classify mosquito species based on common features of their habitats, samples were obtained fortnightly between June 2001-October 2003 in the subtropical province of Chaco, Argentina. Data on the type of larval habitat, nature of the habitat (artificial or natural), size, depth, location related to sunlight, distance to the neighbouring houses, type of substrate, organic material, vegetation and algae type and their presence were collected. Data on the permanence, temperature, pH, turbidity, colour, odour and movement of the larval habitat's water were also collected. From the cluster analysis, three groups of species associated by their degree of habitat similarity were obtained and are listed below. Group 1 consisted of Aedes aegypti. Group 2 consisted of Culex imitator, Culex davisi, Wyeomyia muehlensi and Toxorhynchites haemorrhoidalis separatus. Within group 3, two subgroups are distinguished: A (Psorophora ferox, Psorophora cyanescens, Psorophora varinervis, Psorophora confinnis, Psorophora cingulata, Ochlerotatus hastatus-oligopistus, Ochlerotatus serratus, Ochlerotatus scapularis, Culex intrincatus, Culex quinquefasciatus, Culex pilosus, Ochlerotatus albifasciatus, Culex bidens) and B (Culex maxi, Culex eduardoi, Culex chidesteri, Uranotaenia lowii, Uranotaenia pulcherrima, Anopheles neomaculipalpus, Anopheles triannulatus, Anopheles albitarsis, Uranotaenia apicalis, Mansonia humeralis and Aedeomyia squamipennis). Principal component analysis indicates that the size of the larval habitats and the presence of aquatic vegetation are the main characteristics that explain the variation among different species. In contrast, water permanence is second in importance. Water temperature, pH and the type of larval habitat are less important in explaining the clustering of species.

Detection of enteric viruses in recreational waters of an urban lagoon in the city of Rio de Janeiro, Brazil

The aim of this study was to determine the occurrences of the group A rotavirus (RVA), norovirus (NoV) and human adenovirus (HAdV) in the surface waters of an urban lagoon (Rodrigo de Freitas Lagoon) in the city of Rio de Janeiro, Brazil. During one year of surveillance, water samples were obtained from the lagoon and other interconnected ecosystems (river and beach). The samples were concentrated using an adsorption-elution method with a negatively charged membrane and tested by qualitative and quantitative polymerase chain reaction assays. RVA was the most prevalent virus detected (24.3%) with a viral load ranging from 3.0 x 10¹-5.6 x 10(4) genome copies/L, followed by NoV (18.8%) and HAdV (16.7%). Considering water samples suitable for bathing, according to Escherichia coli criterion (< 2,000 most probable number/100 mL), viruses were detected in 50% (57/114) of them. Physicochemical parameters were also measured and showed possible correlations between turbidity and RVA presence and between pH and NoV presence. These data demonstrate the importance of considering viral parameters to ensure water quality and the utilisation of these parameters as additional tools for the characterisation of environmental contamination.

Testing the response of macroinvertebrate functional structure and biodiversity to flooding and confinement

The aim of the present study was to investigate the relative importance of flooding- and confinement-related environmentalfeatures in explaining macroinvertebrate trait structure and diversity in a pool of wetlands located in a Mediterranean riverfloodplain. To test hypothesized trait-environment relationships, we employed a recently implemented statistical procedure, thefourth-corner method. We found that flooding-related variables, mainly pH and turbidity, were related to traits that confer an abilityof the organism to resist flooding (e.g., small body-shape, protection of eggs) or recuperate faster after flooding (e.g., short life-span, asexual reproduction). In contrast, confinement-related variables, mainly temperature and organic matter, enhanced traits that allow organisms to interact and compete with other organisms (e.g., large size, sexual reproduction) and to efficiently use habitat and resources (e.g., diverse locomotion and feeding strategies). These results are in agreement with predictions made under the River Habitat Templet for lotic ecosystems, and demonstrate the ability of the fourth-corner method to test hypothesis that posit traitenvironment relationships. Trait diversity was slightly higher in flooded than in confined sites, whereas trait richness was not significantly different. This suggests that although trait structure may change in response to the main environmental factors, as evidenced by the fourth-corner method, the number of life-history strategies needed to persist in the face of such constraints remains more or less constant; only their relative dominance differs

Quality of surface water related to land use: a case study in a catchment with small farms and intensive vegetable crop production in southern Brazil

Water degradation is strongly related to agricultural activity. The aim of this study was to evaluate the influence of land use and some environmental components on surface water quality in the Campestre catchment, located in Colombo, state of Parana, Brazil. Physical and chemical attributes were analyzed (total nitrogen, ammonium, nitrate, total phosphorus, electrical conductivity, pH, temperature, turbidity, total solids, biological oxygen demand, chemical oxygen demand and dissolved oxygen). Monthly samples of the river water were taken over one year at eight monitoring sites, distributed over three sub-basins. Overall, water quality was worse in the sub-basin with a higher percentage of agriculture, and was also affected by a lower percentage of native forest and permanent preservation area, and a larger drainage area. Water quality was also negatively affected by the presence of agriculture in the riparian zone. In the summer season, probably due to higher rainfall and intensive soil use, a higher concentration of total nitrogen and particulate nitrogen was observed, as well as higher electrical conductivity, pH and turbidity. All attributes, except for total phosphorus, were in compliance with Brazilian Conama Resolution Nº 357/2005 for freshwater class 1. However, it should be noted that these results referred to the base flow and did not represent a discharge condition since most of the water samples were not collected at or near the rainfall event.

HEAVY METALS AND MICRONUTRIENTS IN THE SOIL AND GRAPEVINE UNDER DIFFERENT IRRIGATION STRATEGIES

Soils under natural conditions have heavy metals in variable concentrations and there may be an increase in these elements as a result of the agricultural practices adopted. Transport of heavy metals in soil mainly occurs in forms dissolved in the soil solution or associated with solid particles, water being their main means of transport. In this context, the aim of this study was to evaluate the heavy metal and micronutrient content in the soil and in the grapevine plant and fruit under different irrigation strategies. The experiment was carried out in Petrolina, PE, Brazil. The treatments consisted of three irrigation strategies: full irrigation (FI), regulated deficit irrigation (RDI), and deficit irrigation (DI). During the period of grape maturation, soil samples were collected at the depths of 0-10, 10-20, 20-40, 40-60, and 60-80 cm. In addition, leaves were collected at the time of ripening of the bunches, and berries were collected at harvest. Thus, the heavy metal and micronutrient contents were determined in the soil, leaves, and berries. The heavy metal and micronutrient contents in the soil showed a stochastic pattern in relation to the different irrigation strategies. The different irrigation strategies did not affect the heavy metal and micronutrient contents in the vine leaves, and they were below the contents considered toxic to the plant. In contrast, the greater availability of water in the FI treatment favored a greater Cu content in the grape, which may be a risk to vines, causing instability and turbidity. Thus, adoption of deficit irrigation is recommended so as to avoid compromising the stability of tropical wines of the Brazilian Northeast.

Seasonal patterns op biomass variation of Ruppia cirrhosa (Petagna) Grande and Potamageton pectinatus L. in a coastal logoon

Coastal lagoons where salinity varies within a wide range during the year are colonized by euryhaline macrophytes which can develop extensive beds. Seasonal changes in biomass of Ruppia cirrhosa and Potamogeton pectinatus were studied in Tancada Lagoon (Ebro Delta, NE Spain) in order to reveal the environmental factors controlling their population development. Ruppia cirrhosa occupy a larger area of the lagoon than Potarnogeton pectinatus. Their maximum above ground biomasses are also different (495 g m-2 and 351 g m-2 ash free dry weight, respectively). Below ground biomass of Ruppia cirrhosa is between 9 and 53 % of the above ground biomass, while it is 3-40 % for Potamogeton pectinatus. Chlorophyll a contents show fluctuations similar to biomass. Low salinity and high turbidity caused by freshwater inflows favour Potamogeton expansion, while Ruppia development is favoured by high salinity and transparent water.

08012-008 Lake Morris Final Report

Lake Morris is the larger of two lakes which serve as the municipal water supply for the City of Chariton, Iowa. As a site for fishing and boating, it also serves as a significant recreational resource for area residents. Its ability to sustain these uses has been significantly impaired by long-term and ongoing accumulation of sediment and sediment-borne nutrients from both public and private land within the watershed. This accumulation has resulted in reduced water depth, reduced water-holding capacity, reduced quality of the fishery, increased water turbidity, increased growth of undesirable algae, and increased cost of treating the water for municipal uses. Water quality projects undertaken in the past, notably the Lucas Lakes Project of the 1990s, made important progress in reducing sedimentation from privately-owned land higher in the watershed, but paid little attention to land owned by the City of Chariton immediately surrounding the lakes. A recent reassessment of gully erosion within the watershed shows serious, ongoing erosion on that City-owned land. This project proposes a two-part approach to improving the water quality in Lake Morris. First, we propose that a complement of five SolarBee water circulation devices be installed in Lake Morris to provide near-term and continuing improvements in water quality, by inhibiting cyanobacterial growth and thereby removing the need for treatment of the lakes with copper sulfate. Second, we propose the installation of erosion-control structures on primarily City-owned land surrounding the lake, to provide a major reduction in ongoing sedimentation.

Avaluació dels canvis de ritme de sedimentació en el canyó submarí d'Arenys a partir del Pb-210

Amb la finalitat d’estudiar els ritmes d’acumulació de sediments durant els últims 100 anys, s’han extret tres testimonis de sediments del canyó d’Arenys a profunditats de 1074 m, 1410 m i 1632 m respectivament. Els ritmes de sedimentació basats en els perfils verticals de Pb-210 suggereixen que les tendències actuals sobre el flux i acumulació de sediments poden ser diferents a tendències passades. Durant la dècada dels 70 es va portar a terme una ràpida evolució de la flota pesquera del port d’Arenys de Mar. Aquest fet es pot relacionar amb els canvis en el ritme d’acumulació dels sediments al testimoni extret a 1074 m. Els flancs del canyó submarí són objectiu dels arrossegadors del port d’Arenys de Mar, una activitat que pot fer variar la morfologia del fons marí, la resuspensió de les partícules i pot crear fluxos de terbolesa. Per tant, els resultats suggereixen que l’activitat pesquera d’arrossegament pot afectar als ambients submarins d’una manera més important del que s’havia pensat.

Mixing of Rhône River water in Lake Geneva (Switzerland-France) inferred from stable hydrogen and oxygen isotope profiles

Depth profiles were sampled at different locations throughout Lake Geneva on a monthly and seasonal basis over the course of 2 years and analysed for their stable hydrogen and oxygen isotope compositions. The isotopic compositions indicate an isotopic stratification in the metalimnion during summer and fall. This is related to mixing of Rhône River water, which in summer is dominated by snow and glacier melt waters, and lake water, with the latter having a homogenous isotopic composition. The observed interflow layer is 7-15 m thick and can be traced by the distinct stable isotope composition of the water for about 55 km throughout the lake as well as into shallow bay regions. Depth of the interflow layer close to the Rhône River mouth is similar to those previously described based on echo-soundings and turbidity profiles of sediment dispersion. In contrast to previous descriptions of the interflow within Lake Geneva, the stable isotope compositions allow for direct, natural tracing of the Rhône River water even in cases where the turbidity and conductivity measurements do not indicate such an interflow. In addition, the method allows for a quantification of the Rhône River and lake water in the interflow with the fraction of Rhône River water within the interflow estimated to be up to 37% in summer. The isotopic composition further indicates different vertical mixing processes within the two lake basins of Lake Geneva, related to the density gradients and local stability within the water column. The method may be applicable to other lakes in catchments with large differences in the topography as water that originates from high altitudes or glaciers has a distinct oxygen and hydrogen isotope composition compared to other sources of water originating at lower altitudes and/or from direct precipitation over the lake. Stable isotope measurements thus improve the understanding of the circulation of water within the lake, which is fundamental for an evaluation of the water residence times, dissolved pollutant and nutrient transport as well as oxygenation.

1003-001 Williamson Pond Final Report

Williamson Pond is a 26-acre publicly owned lake located about 2 miles east of the town of Williamson, in Lucas County. It has a watershed area of 1,499 acres. It has been managed since 1976 by the Lucas County Conservation Board (while still under state ownership) for fishing, boating, hunting, picnicking and other passive uses. Designated uses are Class AI, primary contact, and Class B (LW) aquatic life. Williamson Pond is on the 2004 EPA 303(d) List of Impaired Waters. A Total Maximum Daily Load (TMDL) for turbidity and nutrients at Williamson Pond was prepared by IDNR in 2005 and approved by EPA in 2006. The TMDL set reduction targets for both suspended sediment and phosphorus. The Williamson Pond Watershed Management Plan has provided the local work group and partners with information to develop and implement strategies to improve and protect water quality. These strategies are based on a three phase approach that will ultimately lead the removal of Williamson Pond from the Impaired Waters List. The goals identified in this proposal (Phase I) will reduce sediment and phosphorus delivery by 453 tons and 589 pounds annually. The Lucas County SWCD has and will continue to provide leadership on the Williamson Pond Project and has secured the partnerships necessary to address water quality problems and hired a part-time project coordinator to manage, implement, and oversee all activities pertaining to this proposal.

09012-009 Little River Lake Watershed Final Report

Little River Lake watershed is a 13,305 acre subwatershed of Little River. The 788 acre lake was listed as a 303d impaired water body in 2008 due to elevated turbidity and algae levels. The Decatur SWCD has prioritized water quality protection efforts within the Little River Lake watershed because 1) portions of this watershed has been identified as the primary contributor of sediment and nutrients to Little River Lake, which provides an essential source of drinking water for Decatur County and the Southern Iowa Rural Water Association; 2) the watershed provides exemplary education and project interpretation opportunities due to its proximity to Little River Lake Recreation Area, and 3) by using targeted and proven soil conservation practices to address water quality deficiencies the probability of successfully attenuating soil erosion and ameliorating water quality impairments is enhanced. The specific goals of this proposal are to: 1. reduce annual sediment, and phosphorous delivery to the lake by 11,280 tons and 14,664 lbs., respectively, via applications of conservation practices on targeted agricultural land; 2. delist the lake as an EPA 303d impaired water body via water quality enhancement; 3. obtain a “Full Support” status for the lake’s aquatic life and recreational use; 4. reduce potable water treatment costs (minimum 50% cost reduction) associated with high suspended solid levels; and 5. restore a viable sport-fish population, thereby bolstering tourism and the economy. To achieve timely project implementation the Decatur SWCD has cooperated with the IDNR Watershed Improvement Section, Fisheries Bureau, and IDALS-DSC to assess extant water quality and watershed conditions, coalesced a diverse team of committed partners and secured matching funding from multiple sources.

1017-011 Tuttle Lake Watershed Final Report

The Tuttle Lake Watershed is approximately 125,000 acres and Tuttle Lake itself is 2,270 acres; 5,609 acres of the watershed lies in Iowa territory within Emmet County. It is a sub-watershed of the larger East Fork Des Moines River Watershed, also referred to as Hydrologic Unit Code 07100003. For the purpose of this document, grant money is only being applied for the project implementation in the Iowa portion of the Tuttle Lake Watershed. Tuttle Lake was placed on the 2002 EPA 303(d) Impaired Waters List due to a “very large population of suspended algae and very high levels of inorganic turbidity.” In 2004, the Iowa Department of Natural Resources (IDNR) completed a Total Maximum Daily Load (TMDL) study on Tuttle Lake and found excess sediment and phosphorus levels being the primary pollutants causing the algae and turbidity impairment. Although two point sources were located in Minnesota, IDNR determined that the influx of nutrients is likely from agricultural runoff and re-suspension of lake sediment. The condition of Tuttle Lake is such that the reduction of sediment, nutrients [phosphorus and nitrogen] and pathogens is the primary objective. To achieve that objective, wetlands will be constructed in this first phase to reduce the delivery of nitrogen, phosphorus, and sediment to Tuttle Lake.

08009-005 Storm Lake Watershed Final Report

Storm Lake, the state's fourth largest natural lake, has been the centerpiece of significant economic development, including a resort, water park, and state marina. While there have been considerable improvements to the lake's water quality through a major on-going lake restoration program and watershed project, the Little Storm Lake area still needs to be addressed. Little Storm Lake is a 190 acre area on the nmihwest side of Storm Lake. The water level in both areas is based on the dam height located on the southeast corner of Storm Lake. Approximately 70% of the water from the watershed flows through Little Storm Lake. Little Storm Lake originally had the ability to remove much of the sediment and nutrients from incoming waters. However, due to degradation, proper wetland function has been compromised. Under normal hydrologic conditions Little Storm Lake has the potential to function as a sediment trap for Storm Lake, but tllis capacity is overwhelmed during high flows. Little Storm Lake is at or near its sediment trapping capacity, which results in higher sediment transport into Storm Lake. Resuspension of sediments due to wind and other in-lake dynamics, such as rough fish, further exacerbate the total turbidity from suspended sediment and results in movement of sediment from Little Storm Lake into Storm Lake. This project includes a fish barrier and water retention structure between Little Storm Lake and Storm Lake and the construction of a pumping station and associated equipment. The project involves periodic dewatering of Little Storm Lake during years of favorable climatological conditions to consolidate the sediments and revegetate the area. Construction of the fish banier would aid restoration efforts by preventing rough fish from destroying the vegetation and would decrease recruitment of rough fish by limiting their spawning area. In the future, if the diminished trapping capacity of Little Storm Lake still results in sediment moving into Storm Lake, a dredging project would be initiated to deepen Little Storm Lake.