Vooruzhennyi︠a︡ sily Rosī.

Mode of access: Internet.

Revolución de Entre-Ríos. La guerra y la paz.

Mode of access: Internet.

Prestigios de la ciega autoridad y del espíritu sistemático que se oponen á la verdadera observación : discurso inaugural que en la abertura de clases del Colegio Nacional de Cirugía Médica de Barcelona leyó...el día 1 de octubre del año 1817 : Ignacio Ameller y Ros.

Mode of access: Internet.

[Deberes que el médico tiene contraidos con la humanidad] : discurso leído ante el claustro de la Universidad Central por el Licenciado en Medicina Don José Montero y Ríos, en el acto de recibir la investidura de Doctor en la misma Facultad.

Mode of access: Internet.

Opyt obozri︠e︡nīi︠a︡ zhizni sanovnikov, upravli︠a︡vshikh inostrannymi di︠e︡lami v Rosīi /

-- ch. 3. Vit︠s︡e-kant︠s︡lery

[Diodōros] = Diodori Siculi Bibliothecae historicae libri qui supersunt /

"Notitia literaria editionum Diodori Siculi": v. 1, p. CLX-CLXXI.

D'Artagnan, Capitaine des mousquetaires du roi ; histoire vèridique d'un héros de roman.

Mode of access: Internet.

Hē kainē diathēkē tou kyriou kai sōtēros hēmōn Iēsou Christou : metaphrastheisa ek tou Hellēnikou.

Mode of access: Internet.

Klēros kai theatron /

Includes bibliographical references.

Pami︠a︡tniki diplomaticheskikh snoshenīĭ drevneĭ Rosīi s derzhavami inostrannymi.

No more published.

Obreros rurales transitorios. El caso de los cosecheros de arándano en Concordia, Entre Ríos

El cultivo de arándano en Argentina, prácticamente desconocido dos décadas atrás, se desarrolla incipientemente durante la década del noventa y experimenta un acelerado crecimiento tras la salida de la convertibilidad y la devaluación de la moneda en 2002. Actualmente, la superficie implantada alcanza las 4200 hectáreas, la mitad de las cuales, aproximadamente, se concentra en la provincia de Entre Ríos, principalmente en la localidad de Concordia. Se trata de una producción con altos requerimientos de mano de obra transitoria, especialmente para el período de cosecha, que se realiza manualmente y se extiende a lo largo de apenas dos meses. Nuestro interés se centra en analizar las condiciones laborales y de vida de los obreros cosecheros del arándano en la localidad de Concordia. Como primera aproximación a la temática, la presente ponencia tiene por objetivo realizar un estado de la cuestión, examinando las características del mercado de trabajo conformado alrededor de esta producción

Direct inhibitory effects of an antisense oligodeoxynucleotide upon the volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells

The effects of a 15-mer antisense c-myc phosphorothioate modified oligodeoxynucleotide (OdN) upon the volume-sensitive Cl- current in ROS 17/2.8 cells were investigated using the whole-cell configuration of the patch clamp technique. At 5 microM, the OdN reversibly inhibited the current in a voltage- and time-dependent fashion. This was evident from the reduction in the peak current as assessed at the termination of each voltage pulse and an acceleration of the time-dependent inactivation present at strongly depolarised potentials. The kinetic modifications induced by the OdN suggest it may act by blocking the pore of open channels when the cell membrane potential is depolarised.

Effects of arachidonic acid upon the volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells.

1. The effects of arachidonic acid upon the volume-sensitive Cl- current present in cultured osteoblastic cells (ROS 17/2.8) was studied using the whole-cell patch-clamp technique. 2. Arachidonate produced two distinct phases of inhibition, a rapid phase occurring within 10-15 s of application preceding a slower phase that occurred 2 min after onset of arachidonate superfusion. Accompanying the slower inhibitory phase was an acceleration of the time-dependent inactivation exhibited by the current at strongly depolarized potentials (> + 50 mV). The half-maximal inhibitory concentrations (IC50) were 177 +/- 31 and 10 +/- 4 microM for the two phases respectively. 3. Arachidonate was still effective in the presence of inhibitors of cyclo-oxygenase (indomethacin, 10 microM), lipoxygenase (nordihydroguaretic acid, 10-100 microM) and cytochrome P450 (SKF525A, 100 microM; ethoxyresorufin, 10 microM; metyrapone, 500 microM; piperonyl butoxide, 500 microM; cimetidine, 1 mM). The effects of arachidonate could not be produced by another cis unsaturated fatty acid, oleic acid. 4. Measurements of cell volume showed that arachidonate effectively inhibited the regulatory volume decrease elicited by ROS 17/2.8 cells in response to a reduction in extracellular osmolarity. 5. It is concluded that the volume-sensitive Cl- conductance in ROS 17/2.8 cells is directly modulated by arachidonate and may represent a physiological mechanism by which volume regulation can be controlled in these cells.

Characterization of a volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells

1. During osmotic swelling, cultured osteoblastic cells (ROS 17/2.8) exhibited activation of large amplitude Cl- currents in the whole-cell configuration of the patch-clamp technique. Effects of hypotonic shock on cell volume and membrane conductance were rapidly reversed on return to isotonic conditions. 2. Voltage command pulses in the range -80 to +50 mV produce instantaneous activation of Cl- currents. At potentials more positive than +50 mV the current exhibited time-dependent inactivation. The instantaneous current-voltage relationship was outwardly rectifying. 3. The anion permeability sequence of the induced current was SCN- (2.2) > I- (1.9) > Br- (1.5) > Cl- (1.0) > F- (0.8) > gluconate- (0.2). This corresponds to Eisenman's sequence I. 4. The volume-sensitive Cl- current was effectively inhibited by the Cl- channel blockers 4,4'-diisothiocyanatostilbene-2,2-disulphonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB). Outward currents were more effectively suppressed by DIDS than inward currents. The concentrations for 50% inhibition (IC50) of outward and inward currents were 81 and 298 μM, respectively. NPPB was equally effective at inhibiting outward and inward currents (IC50 of 64 μM). The current was relatively insensitive to diphenylamine-2-carboxylate (DPC), 500 μM producing only 22.5 ± 4.0% inhibition. 5. Inhibitors of protein kinase A (H-89, 1 μM) and tyrosine kinase (tyrphostin A25, 200 μM) were without effect upon activation of Cl- currents in response to hypotonic shock. Under isotonic conditions, elevation of intracellular Ca2+ by ionomycin (1 μM) or activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate (TPA, 0.1 μM) failed to evoke increases in basal Cl- conductance levels. 6. It is concluded that an outwardly rectifying Cl- conductance is activated upon osmotic swelling and may be involved in cell volume regulation of ROS 17/2.8 cells.