Growing banana prawns in prawn farm settlement ponds to utilise and help remove waste nutrients.

To assess their utility for profitable wastewater bioremediation, banana prawns, Penaeus (Fenneropenaeus) merguiensis (de Man), were stocked at low densities (1 – 5 m-2) and grown without supplemental feeding in five commercial-prawn-farm settlement ponds (0.3 to 6.0 ha). The prawns free-ranged in the variously designed ponds for 160 to 212 days after stocking as PL15. Survival estimates ranged from 12% to 60% with production of 50 – 528 kg ha-1. Over 1150 kg of marketable product was produced in the study. Exceptional growth was monitored at one farm where prawns reached an average size of 17g in 80 days. Nutrients in water flowing into (8 - 40 ML d-1) and out of the settlement pond at that farm were assessed twice weekly along with routine water quality measurements. Only small differences in water qualities were detected between waters running into and out of this settlement pond. Total nitrogen levels gradually increased from 1 - 1.5 mg L-1 early in the season to over 3 mg L-1 towards the end of the season. Total phosphorus levels similarly rose from 0.1 - 0.2 mg L-1 to 0.3 - 0.4 mg L-1 in the middle of the season, but fell to 0.2 – 0.3 mg L-1 towards the end when approximately 12,000 prawns were harvested with a total weight of 175 kg. No significant differences (P > 0.05) were detected in the overall acceptability of prawns harvested from each of the 5 settlement ponds in small-scale consumer sensory analyses. The prawns from settlement ponds were rated similarly to banana prawns grown with commercial diets at two other establishments. Microbiological analyses of prawns from all farms showed bacterial levels to be well within food-grade standards and lower than prawns produced in a normal growout pond. These results demonstrate that high quality food grade banana prawns can be produced in these wastewater treatment systems.

Bivalves for the remediation of prawn farm effluent: identification of some potentially useful species in Southern Queensland

Several species of oysters, clams and mussels are currently being used around the world to create extra profits and help remediate waste-waters from mariculture operations. To identify opportunities and potentially suitable species of bivalves for remediation of prawn farm effluent in Australia, recent literature dealing with bivalve filtration is reviewed, and species occurring naturally in a banana prawn, Penaeus (Fenneropenaeus) merguiensis, grow-out pond and effluent streams at the Bribie Island Aquaculture Research Centre (BIARC) were collected, identified and assessed in terms of their tolerance of high silt loadings over 3 months. Three bivalve species predominated in the BIARC case study. These were the mud ark, Anadara trapezia, the rock oyster, Dendostrea folium, and the pearl shell, Pinctada maculata. The mud ark demonstrated the highest tolerance of silt loading (99% survival), followed by pearl shells and rock oysters (88 and 63% survival respectively).

Organic diet development for black tiger prawns

The survival and growth of black tiger prawn (Penaeus monodon) juveniles (~3.3 g) were compared after feeding in tanks over one month with several prepared diets based on organically certified ingredients. The extrusion process in the manufacture of pelletised experimental diets was similar to processes used in commercial plants and was closely documented. The daily feeding rate (6% of starting mean body weight) was split equally into two feeds, one in the morning and one in the afternoon. All diets tested produced high survival (97-100%). A widely-used commercial Australian prawn feed was used as a control diet. It contained 41.2% protein with 29.5 g kg-1 lysine, and produced the highest (P<0.05) growth (117% weight gain). Three of the experimental organic diets tested (namely, 1. wheat + soy, 2. pig weaner diet + soy, and 3. pig weaner diet + dried fish waste) produced moderate growth (73–77% weight gain). These contained 33%, 36% or 31% protein, respectively, and produced better (P<0.05) growth than diets utilising a range of other prospective ingredients (eg: wheat + dried scallop gut, wheat + fish waste, wheat + chickpea, or wheat + macadamia meal, containing 23%, 25%, 29% or 24% protein, respectively). An unfed control-treatment produced the lowest (P<0.05) growth (4% weight gain). The water stability of the experimental diets that produced the best growth was poorer than the commercial diet, suggesting that improvements in this aspect of these organic feed’s manufacture could result in additional performance benefits and possibly reduced feed wastage. Analyses revealed a linear relationship between diet performance (in terms of weight gains) and the protein and lysine contents of diets. About 70% of diet performance was explained by these factors. The superior performance of the commercial diet could be attributed primarily to its formulation using mainly marine proteins, as well as a range of other unknown factors (commercial in confidence). These other factors range from use of feed attractants, better knowledge of ingredient nutrient availability, different extrusion conditions and the use of other unspecified micro-nutrients not present in the experimental diets. The organic diets studied still require a degree of fine-tuning before structured commercial uptake. This would sensibly include further detailed investigations of the composition and nutrient availabilities of these and other organic dietary ingredients, and refinement of the extrusion process for formulated diets.

Identification of tropomyosin as the major shrimp allergen and characterization of its IgE-binding epitopes

The major heat-stable shrimp allergen (designated as Sa-II), capable of provoking IgE-mediated immediate type hypersensitivity reactions after the ingestion of cooked shrimp, has been shown to be a 34-kDa heat- stable protein containing 300 amino acid residues. Here, we report that a comparison of amino acid sequences of different peptides generated by proteolysis of Sa-II revealed an 86% homology with tropomyosin from Drosophila melanogaster, suggesting that Sa-II could be the shrimp muscle protein tropomyosin. To establish that Sa-II is indeed tropomyosin, the latter was isolated from uncooked shrimp (Penaeus indicus) and its physicochemical and immunochemical properties were compared with those of Sa-II. Both tropomyosin and Sa-II had the same molecular mass and focused in the isoelectric pH range of 4.8 to 5.4. In the presence of 6 M urea, the mobility of both Sa-II and shrimp tropomyosin shifted to give an apparent molecular mass of 50 kDa, which is a characteristic property of tropomyosins. Shrimp tropomyosin bound to specific IgE antibodies in the sera of shrimp-sensitive patients as assessed by competitive ELISA inhibition and Western blot analysis. Tryptic maps of both Sa-II and tropomyosin as obtained by reverse phase HPLC were superimposable. Dot-blot and competitive ELISA inhibition using sera of shrimp-sensitive patients revealed that antigenic as well as allergenic activities were associated with two peptide fractions. These IgE-binding tryptic peptides were purified and sequenced. Mouse anti-anti-idiotypic antibodies raised against Sa-II specific human idiotypic antibodies recognized not only tropomyosin but also the two allergenic peptides, thus suggesting that these peptides represent the major IgE binding epitopes of tropomyosin. A comparison of the amino acid sequence of shrimp tropomyosin in the region of IgE binding epitopes (residues 50-66 and 153-161) with the corresponding regions of tropomyosins from different vertebrates confirmed lack of allergenic cross-reactivity between tropomyosins from phylogenetically distinct species.

Aquaculture Asia, Vol. 8, No. 1, pp.1-58, January-March 2003

*Table of Contents* Sustainable Aquaculture Fertilization, soil and water quality management in small-scale ponds part II:Soil and water quality management S. Adhikari Fisheries and aquaculture activities in Nepal Tek Gurung Peter Edwards writes on rural aquaculture: A knowledge-base for rural aquaculture Farmers as Scientists: Commercialization of giant freshwater prawn culture in India M.C. Nandeesha Aquaculture in reservoir fed canal based irrigation systems of India – a boon for fish production K.M. Rajesh, Mridula R. Mendon, K. N. Prabhudeva and P. Arun Padiyar Research and Farming Techniques Production and grow-out of the Black-lip pearl oyster Pinctada margaritifera Idris Lane Breeding of carps using a low-cost, small-scale hatchery in Assam, India: A farmer proven technology S.K. Das Genes and Fish: Hybridisation – more trouble than its worth? Graham Mair Breeding and culture of the sea cucumber Holothuria scabra in Vietnam R. Pitt and N. D. Q. Duy The potential use of palm kernel meal in aquaculture feeds Wing-Keong Ng Using a Simple GIS model to assess development patterns of small-scale rural aquaculture in the wider environment Simon R. Bush Aquaculture fundamentals: Getting the most out of your feed Simon Wilkinson Marine finfish section Status of marine finfish aquaculture in Myanmar U Khin Kolay Regional training course on grouper hatchery production Aquatic Animal Health Advice on aquatic animal health care: Problems in Penaeus monodon culture in low salinity areas Pornlerd Chanratchakool

Spawning pattern of the Nematopalaemon hastatus (Crustacea: Palaemonidae) (Aurivilius, 1898), exploited in the artisanal shrimp fishery in the outer Cross River estuary

Some aspects of the reproduction of Nematopalaemon hastatus in the artisanal shrimp fishery in the outer Cross River estuary (Nigeria) was investigated. 2 kg samples were taken from the daily catch of the N. hastatus fishery on a twice-weekly basis. The N. hastatus in the sample were then observed for gravidity, using a hand lens, and their post-orbital carapace length (POCL) taken. This was done over a 12-month period. The eggs in formalin-fixed samples showed colour changes from light orange to dark brown with maturation. Gravid females with eggs in different stages of maturation, from light orange to dark brown were observed in the samples. The reproduction occurs all year-round. The proportion of gravid females observed in samples had two distinct peaks in June and November, and two distinct troughs in May and October. The lowest length at which gravidity was observed was 6.0 mm POCL. There were no Penaeus notialis in the samples

Survey of the shrimp stocks of the Sierra Leone inshore shelf : a summary report outlining objectives, observations and recommendations on the status of the Sierra Leone shrimp fishery

This report gives the species and catch-rates per hour of some commercial shrimp species caught in the course of a short survey of inshore shelf fishing grounds. Species seen in significant quantities were: Parapenneopsis atlantica, Parapenaeus longirostris, Penaeus kerathurus and Penaeus duorarum notialis

Estimativa da captura total de peixe e camarão das pescarias de emalhe e de arrasto da Baía de Maputo, em 1985

The most important fisheries of Maputo Bay (Mozambique) are the gill netting of the Hilsa kelee and the trawling of the shrimps Penaeus indicus and Metapenaeus monoceros . Data of capture and yields are presented and the number of artisanal fishing boats and semi-industrial fishing vessels is given.

White spot syndrome virus (WSSV) transmission risk through infected cooked shrimp products assessed by polymerase chain reaction (PCR) and bio-inoculation studies

The aim of the study was to evaluate the resistance of white spot syndrome virus (WSSV) in shrimps (Penaeus monodon) to the process of cooking. The cooking was carried out at 1000C six different durations 5, 10, 15, 20, 25 and 30 min. The presence of WSSV was tested by single step and nested polymerase chain reaction (PCR). In the single step PCR, the primers 1s5 & 1a16 and IK1 & IK2 were used. While in the nested PCR, primers IK1 &IK2 – IK3 & IK4 were used for the detection of WSSV. WSSV was detected in the single step PCR with the primers 1s5 and 1a16 and the nested PCR with the primers IK1 and IK2 – IK3 & IK4 from the cooked shrimp samples. The cooked shrimps, which gave positive results for WSSV by PCR, were further confirmed for the viability of WSSV by conducting the bio-inoculation studies. Mortality (100%) was observed within 123 h of intra-muscular post injection (P.I) into the live healthy WSSV-free shrimps (P. monodon). These results show that the WSSV survive the cooking process and even infected cooked shrimp products may pose a transmission risk for WSSV to the native shrimp farming systems.

La pêche crevettière en Côte d'Ivoire. Bilan 1969-1970 et perspectives

The evolution of the fishery of the pink shrimp Penaeus duorarum Burkenroad is analysed from its beginning in 1969 until the end of 1970. A rapid and general decline of the yield has been evident during this period. The actual shrimp fleet seems to be too big to allow an exploitation economically convenient of the stock.

Antibacterial marine bacterium deter luminous vibriosis in shrimp larvae

Inhibitory activity of a marine pigmented bacterium - Alteromonas sp. - isolated from Penaeus monodon Fabricius larva against pathogenic and environmental isolates of Vibrio harveyi was studied. All the isolates were inhibited to varying degrees by Alteromonas sp. in vitro. The antibacterial substance produced by the Alteromonas sp. was soluble in organic solvent and closely bound to the external surface of bacterial cells. The antibacterial Alteromonas sp., when allowed to colonize on shrimp larvae, suppressed the activity of V. harveyi M3 and reduced mortality of P. monodon larvae in vivo.

Role of women in Indian shrimp farming

Women in India are involved in various facets of shrimp (Penaeus monodon; Penaeus indicus) farming, including pond construction, seed collection, collection of feed materials and preparation of feeds, pond maintenance and post-harvest handling. This study indicates that 40% of laborers involved in shrimp farm construction are women. The various roles of women in shrimp farming are also described.

A survey of ichthyofauna of Lake Kanyaboli and other small waterbodies in Kenya: alternative refugia for endangered fish species

In 1988, the World Conservation Union (WCU) Red Book of Endangered Species listed hundreds of endemic fishes of Lake Victoria under a single heading - "ENDANGERED". Most of the endemic native food fishes are either endangered or extinct. However, a survey of the fauna of Lake Kanyaboli, revealed that a few remaining samples of these native fishes are actually thriving. These include several unidentified Haplochromis spp., Oreochromis esculentus and Oreochromis variabilis. As a result, a stock rehabilitation and management strategy has been designed to use Lake Kanyaboli and other small waterbodies as conservation 'Refugia' for endangered fish species of the larger Lake Victoria.

Multiple environmental states affecting penaeid shrimp production in Peru

A modification of the Schaefer surplus-production model was used to account for environmental induced variations of shrimp (Penaeus vannamei) catch in northern Peru. Based on time series of catch, effort, river discharge and sea surface temperature, fluctuations in catch of shrimps are explained and discussed with respect to multiple level of carrying capacity and hence different maximum sustainable yields.

Shrimp disease management using bioactive marine secondary metabolites: an eco-friendly approach

Vibriosis caused by opportunistic and secondary bacterial pathogens is still a serious disease problem in aquaculture of the black tiger shrimp Penaeus monodon. Attempts were made for controlling shrimp bacterial disease using Marine Secondary Metabolites (MSMs). Findings indicated that the MSMs of seaweed Ulva fasciata and Dendrilla nigra are effective for controlling shrimp bacterial pathogens.