Analysis of Protein Turnover by Quantitative SNAP-Based Pulse-Chase Imaging

Assessment of protein dynamics in living cells is crucial for understanding their biological properties and functions. The SNAP-tag, a self labeling suicide enzyme, presents a tool with unique features that can be adopted for determining protein dynamics in living cells. Here we present detailed protocols for the use of SNAP in fluorescent pulse-chase and quench-chase-pulse experiments. These time-slicing methods provide powerful tools to assay and quantify the fate and turnover rate of proteins of different ages. We cover advantages and pitfalls of SNAP-tagging in fixed- and live-cell studies and evaluate the recently developed fast-acting SNAPf variant. In addition, to facilitate the analysis of protein turnover datasets, we present an automated algorithm for spot recognition and quantification.

Influência das redes sociais na intenção de compra das marcas

O presente trabalho de investigação pretende compreender a influência da presença das marcas nas redes sociais, nomeadamente no Facebook, na intenção de compra do consumidor no ponto de venda.É feita uma revisão de literatura à panóplia de conceitos de marketing e comunicação de marketing digital, ao consumidor e à decisão de compra e ao social media online, sendo dado maior enfâse à relação entre consumidor e as marcas presentes no Facebook. A investigação é dividida em duas fases, uma fase qualitativa de análise à estratégia digital das marcas e uma fase quantitativa de análise ao comportamento do consumidor.

Labor turnover : calculation and cost.

Includes bibliographical references.

Political appointees : turnover rates in executive schedule positions requiring Senate confirmation : fact sheet for the Chairman, Committee on Governmental Affairs, U.S. Senate /

"B-249785"--P. [1].

Wisconsin employment, earnings, and labor turnover.

WI docs no.: EMP 1.1/2:4/1963-1971

The turnover of labor : November, 1919.

"Prepared by Boris Emmet"--p. 5.

Turnover analysis manual /

Research conducted by LaSalle Governmental Consultants, Ltd.