Ação antimicrobiana de compostos majoritários presentes em óleos essenciais sobre bactérias causadoras de infecções hospitalares

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e caracterização das Tanases do fungo filamentoso Aspergillus carbonarius

Pós-graduação em Biotecnologia - IQ

Inoculação de sementes com Azospirillum brasilense e Rhizobium tropici e adubação nitrogenada em cobertura em feijoeiro de inverno irrigado em sistema plantio direto

Pós-graduação em Agronomia - FEIS

Multiplicação de Diaphorina citri e de Candidatus Liberibacter asiaticus em híbridos de citros indicados como porta-enxerto

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Diversidade e evolução da microbiota lática autóctone em queijo Muçarela de búfala e aplicação tecnológica dos isolados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Solubilización de fuentes de fósforo asociadas a un compuesto orgánico enriquecido con biofertilizantes

The enrichment of an organic compound with biofertilizers of microorganisms (solubilizing phosphorus bacteria, BSF) can improve the properties of phosphate fertilizers applied to soils to increase nutrient availability. The experimental design was randomized complete blocks with a factorial 4x2 +1 arrangement of treatments: 1) four sources of phosphorus: phosphate rock, natural phosphate, triple superphosphate (TSP) and Cuban phosphate rock; 2) two sources of bio-fertilizer prepared from filter cake (cane waste) enriched with microorganisms and no enrichment; 3) and an absolute control (no filter cake or fertilizer); repeated three times. Data was analyzed with ANOVA and treatment means were compared using Tukey (p <= 0.05). Assessments of available P (Bray-2 and Oniani methodology) and the quantification of total and solubilizing bacteria and fungi in the soil were performed 30, 60 and 90 d after starting the experiment. After 60 d, the population of BSF was lower in the control (p <= 0.05) and the content of P (Bray-2) was higher for SFT (p <= 0.01). The application of the bio-fertilizer increased the P content compared to the control. At 90 d P (Bray-2) increased with the SFT treatment, which also increased (p P in the soil at 60 and 90 d regardless of the presence of microorganism-enriched bio-fertilizer. The use of filter cake enriched with microorganisms associated with different sources of P, and applied to the soil with a high content of P, did not change the soil populations of total and solubilizing bacteria and fungi in the short term. TSP promoted the highest levels of P in the soil, irrespective of the presence of the organic compound enriched with P solubilizing microorganisms.

Desempenho agronômico do milho e do trigo em função da inoculação com azospirillum brasilense e doses e fontes de nitrogênio

Pós-graduação em Agronomia - FEIS

Pasteurelose em juvenis de cobias (Rachycentron canadum) criados em tanques-redes no litoral norte do estado de São Paulo - Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Susceptibilidade antimicrobiana ao metronidazole de microrganismos isolados de periodontite crônica e agressiva

The objective of this study was to evaluate the microbial susceptibility to metronidazole from microorganisms isolated from patients with chronic or aggressive periodontitis. The samples were obtained from 50 patients with periodontitis and microorganisms were isolated onto selective and nonselective culture media, identified by biochemical methods and tested for susceptibility to metronidazole. The results evidenced the susceptibility to metronidazole among gram-negative anaerobes and some intermediate susceptibility and resistance among Grampositive anaerobes and facultatives.

Capacitação de produtores rurais para a melhoria da qualidade do leite cru produzido na região de Araçatuba - SP

This paper reports the results of an extension project carried out at Unesp/Veterinary Medicine course in Araçatuba city with milk farmers of the region. The aim of the project was to supply information to farmers concerning to good quality milk production and, at the same time, to follow up the evolution of milk quality parameters, according to the current Brazilian legislation. Every 45 days, approximately, lectures were presented to milk farmers in Araçatuba city region, approaching chemical and microbiological composition of milk, prevention and detection of mastitis, hygiene proceedings in milking and conservation of milk, cleaning and sanitization operations of facilities and equipments and prevention of adulteration. During the intervals between lectures, milk samples were collected from collective milk cooling tanks and analyzed for microbiological, hygienic and physicochemical tests. The main inadequacies in milk quality were high total bacteria and somatic cells counts, low solids contents and water addition. These problems did not proceed to betterment during the project time. So, it was concluded that the time for instruction of farmers was not enough for a progress in the quality of the milk produced in the region, pointing out the need in continuing this kind of work.

Periodontite ulcerativa necrosante: tratamento fisico da superficie radicular com Er YAG laser

Necrotizing ulcerative periodontitis is associated with bacteria and predisposing risk factors. The treatment is centered in elimination and/or control of the bacteria and predisposing risk factors. However the disease acute phase could induce sequelae as periodontal tissue destruction which may be treated by periodontal surgical procedures.

Susceptibility of microorganisms isolated from necrotic pulps to calcium hydroxide

Calcium hydroxide has been used as pulp-capping agent and canal dressing due to its antimicrobial and anti-inflammatory properties besides its ability to induce formation of mineralized tissues. The aim of this study was to evaluate the susceptibility to calcium hydroxide of 146 bacterial strains isolated from endodontic infections. MIC was determined by using an agar dilution method, while contact bactericide activity was performed through in broth. All the isolates were sensitive to calcium hydroxide in concentrations that varied from 0.5mg/ml to 128 mg/ml, and the genera Enterococcus, Pseudomonas, Staphylococcus and Actinomyces were the most resistant. Gramnegative anaerobes proved to be the most sensitive isolates. All the isolates were inhibited after 60 minutes of contact with the alkali in concentration of 100mg/ml

Antimicrobial activity of plants infusions on oral fusobacteria and their adherence to human erythrocytes

Periodontal disease is the result of the interrelationship between microbiotic aggression and the host’s organic defence. Amongst the microorganisms involved in periodontopathies, Fusobacterium nucleatum is conspicuous by establishing a link between the initial and final colonizers, besides producing toxic compounds and adhering to the host’s cells. Control of bacterial biofilm can be achieved by use of chemical agents, many of which extracted from plants. Thus the object of this study was to evaluate the inhibitory activity in vitro of some teas, generally taken in a normal diet, on Fusobacterium nucleatum and your adherence to host’s cells. Minimum inhibitory and bactericidal concentrations were established and haemagglutinative test in microplaques was effected. It was ascertained that all plant extracts have inhibitory activity and that infusions of Camellia sinensis (black tea and green tea), Mentha piperita (mint) and Pimpinella anixem (aniseed) added to the bacteria/erythrocyte compound reduced significantly the adherence of microorganisms.

Chronic osteomyelitis of the maxilla and mandible: microbiological and clinical aspects

Osteomyelitis of the mandible and maxilla are common in developing countries and their treatment may be long-standing and difficult. Thus, the aim of this study was to discuss the main biological aspects of the chronic osteomyelitis of the jaws of especial interest for dental team. These infections are associated with a complex microbiota composed mainly by anaerobic bacteria, sometimes associated with microorganisms originated from the skin and digestive tract. These data suggest that chronic osteomyelitis of the mandible and maxilla should be treated as anaerobic infections in most cases. In addition, local surgical treatments are relevant in the therapy outcome, associated to the use of antimicrobial agents, and the failure to accomplish them is a major cause of treatment failure.

Experimentally induced anachoresis in the periapical region after root canal filling

Anachoresis is the phenomenon through which blood-borne bacteria, dyes, pigments and other materials are attracted and fixed to circumscribed areas of inflammation. This study evaluated the occurrence of anachoresis in the periapical region of dogs submitted to root canal fillings. One hundred and four roots from four dogs were endodontically treated and root canals were filled with zinc-oxide-eugenol cement. Fifty percent were filled up to the dentinocemental junction and the others were overfilled. At 120 days after root canal treatment, experimental bacteremia was induced by intravenous inoculation of 105 CFU Streptococcus pyogenes. The dogs were sacrificed 48 hours and 30 days after the bacteremia. Culture and DNA amplification by PCR revealed the presence of the inoculated bacteria just in periapical tissues of dogs sacrificed 48 hours after bacteremia and not in animals sacrificed after 30 days. AP-PCR fingerprints of recovered colonies of S. pyogenes and the presence of genetic markers of resistance to antimicrobials were similar to the inoculated strain. Endodontically treated periapices seemed to be prone to the occurrence of anachoresis and there was no relationship between the phenomenon and the level of root canal filling.