Estudo da associação de água residuária de suinocultura e adubação mineral na cultura do milho e no solo

RESUMOO uso de água residuária em solo agrícola pode-se caracterizar como uma fonte alternativa de adubação, além de melhorar as condições edáficas do solo devido à matéria orgânica adicionada. Neste contexto, este trabalho teve por objetivo avaliar os efeitos na cultura do milho e em Latossolo Vermelho distroférrico típico, devido à aplicação de água residuária de suinocultura associada com adubação química. No experimento, utilizou-se de cinco doses de ARS (0; 112,5; 225; 337,5 e 450 m3 ha-1) durante o ciclo do milho, combinadas com duas adubações químicas (50 e 75% da dose recomendada), para compor os respectivos tratamentos, com três repetições. Os parâmetros da cultura do milho avaliados foram: produtividade (PROD), altura de plantas (AP), teor de macronutrientes (N, P, K e Mg) e de micronutrientes (Fe, Zn, Cu e B). Os parâmetros de solo avaliados foram: conteúdo de matéria orgânica (MO), soma e saturação por bases (SB e V), pH e teores disponíveis de N, P, K, Cu, Zn, Fe e Mn. A partir dos resultados obtidos, concluiu-se que: na cultura do milho, a aplicação de água residuária da suinocultura é fator predominante no aumento da produtividade e na nutrição mineral em K, Mg, Fe, Cu e B, e insuficiente para N e P; no solo, de modo geral, a água residuária da suinocultura propiciou aumentos positivos nos parâmetros de P, Zn, Cu, pH e SB, negativos nos teores de K e Fe, e nenhum efeito nos parâmetros N, V, MO e Mn; os parâmetros P, Cu e Zn aumentaram significativamente os teores no solo ao longo do experimento, indicando a importância do fator tempo nesse tipo de estudo.

Meso e macrofauna de solo cultivado com milho e irrigado com água residuária da suinocultura

RESUMO A utilização da fauna edáfica como bioindicadora de alterações antrópicas e ambientais vem crescendo nos últimos anos. No entanto, ainda são poucos os estudos em longo prazo que relacionam esses organismos com aplicação de resíduos orgânicos no solo. O objetivo deste trabalho foi avaliar, em dois ciclos da cultura do milho, os efeitos da aplicação de diferentes doses e tipos de águas residuárias da suinocultura (ARS) combinadas ou não com adubação mineral sobre a meso e a macrofauna de solo. Quatro doses (0; 100; 200 e 300 m3ha-1) de ARS, tratada em biodigestor no primeiro ano de estudo e bruta no segundo ano, associadas ou não com adubação mineral, foram aplicadas em dois ciclos da cultura de milho, totalizando 24 parcelas experimentais, sendo 12 com utilização de adubação mineral. Armadilhas de queda foram instaladas em cada uma das parcelas para amostrar a fauna edáfica, sendo os resultados encontrados avaliados por técnicas de estatística multivariada (NMS e Per-MANOVA). A adição de ARS, tanto bruta quanto tratada em biodigestor e de adubação mineral, não influenciou a fauna de solo. Os parâmetros químicos do solo diferenciaram a meso e a macrofauna edáfica somente entre os anos.

Asiakastarpeiden kartoitus kuljetusten häiriöiden analysoinnin ja hallinnan kehittämiseksi

Tämä diplomityö on tehty osana Logproof-tutkimushanketta, joka keskittyy häiriöttömyyden hallintaan logistisissa monitoimijaverkostoissa. Työn tavoitteena on selvittää logistiikkaintensiivisten yritysten tarpeita kuljetusten häiriöiden analysoinnin ja hallinnan kehittämiseksi ja siten ennakoivan riskienhallinnan edistämiseksi. Asiakastarvetietoa on kerätty hyödyntäen puolistrukturoituja haastatteluja ja tietoa on analysoitu käyttäen hyväksi sisällönanalyysiä sekä tulkintataulukkoa. Kiinnostus kuljetusten häiriöitä ja niiden analysointia kohtaan on kasvamassa ja yrityksissä tiedostetaan hallintajärjestelmien ja analysoinnin tarve tulevaisuudessa. Kirjallisuuskatsauksen ja asiakastarpeiden kartoituksen avulla työssä on selvitetty yritysten nykytilaa kuljetusten häiriöiden hallinnan ja analysoinnin osalta ja tarkasteltu mahdollisia tulevaisuuden kehityssuuntia analysoimalla asiakkaiden näkyviä ja piileviä tarpeita. Työssä on edellisten lisäksi tarkasteltu, kuinka havaitut asiakastarpeet ovat sovitettavissa yhteen case-yrityksen, Oy Lars Krogius Ab:n, ARS (Analytic Rou-ting Solution) -palvelun ominaispiirteiden kanssa. Työ tarjoaa tarvetietoa palvelun tulevaisuuden kehitykselle.

Muscarinic toxins : characterization of adrenoceptor binding and applicability of membrane anchoring via glycosylphosphatidylinositol tail

Adrenoceptors (ARs), G-protein coupled receptors (GPCRs) at the plasma membrane, respond to endogenous catecholamines noradrenaline and adrenaline. These receptors mediate several important physiological functions being especially important in the cardiovascular system and in the regulation of smooth muscle contraction. Impairments in the function of these receptors can thus lead to severe diseases and disorders such as to cardiovascular diseases and benign prostatic hyperplasia. The Eastern green mamba (Dendroaspis angusticeps) venom has been shown to contain toxins that can antagonize the functions of GPCRs. The most well-known are muscarinic toxins (MTs) targeting muscarinic acetylcholine receptors (mAChRs) with high affinity and selectivity. However, some reports have indicated that these toxins might also act on the α1- and α2-ARs which can be divided into various subtypes; the α1-ARs to α1A-, α1B- and α1D-ARs and α2-ARs to α2A-, α2B- and α2C-ARs. In this thesis, the interaction of four common MTs (MT1, MT3, MT7 and MTα) with the adrenoceptors was characterized. It was also evaluated whether these toxins could be anchored to the plasma membrane via glycosylphosphatidylinositol (GPI) tail. Results of this thesis reveal that muscarinic toxins are targeting several α-adrenoceptor subtypes in addition to their previously identified target receptors, mAChRs. MTα was found to interact with high affinity and selectivity with the α2B-AR whereas MT7 confirmed its selectivity for the M1 mAChR. Unlike MTα and MT7, MT1 and MT3 have a broad range of target receptors among the α-ARs. All the MTs characterized were found to behave as non-competitive antagonists of receptor action. The interaction between MTα and the α2B-AR was studied more closely and it was observed that the second extracellular loop of the receptor functions as a structural entity enabling toxin binding. The binding of MTα to the α2B-AR appears to be rather complex and probably involves dimerized receptor. Anchoring MTs to the plasma membrane did not interfere with their pharmacological profile; all the GPI-anchored toxins created retained their ability to block their target receptors. This thesis shows that muscarinic toxins are able to target several subtypes of α-ARs and mAChRs. These toxins offer thus a possibility to create new subtype specific ligands for the α-AR subtypes. Membrane anchored MTs on the other hand could be used to block α-AR and mAChR actions in disease conditions such as in hypertension and in gastrointestinal and urinary bladder disorders in a cell-specific manner and to study the physiological functions of ARs and mAChRs in vivo in model organisms.

Cell Model Systems in Characterizing Alpha2-Adrenoceptors as Drug Targets.

The three alpha2-adrenoceptor (alpha2-AR) subtypes belong to the G protein-coupled receptor superfamily and represent potential drug targets. These receptors have many vital physiological functions, but their actions are complex and often oppose each other. Current research is therefore driven towards discovering drugs that selectively interact with a specific subtype. Cell model systems can be used to evaluate a chemical compound's activity in complex biological systems. The aim of this thesis was to optimize and validate cell-based model systems and assays to investigate alpha2-ARs as drug targets. The use of immortalized cell lines as model systems is firmly established but poses several problems, since the protein of interest is expressed in a foreign environment, and thus essential components of receptor regulation or signaling cascades might be missing. Careful cell model validation is thus required; this was exemplified by three different approaches. In cells heterologously expressing alpha2A-ARs, it was noted that the transfection technique affected the test outcome; false negative adenylyl cyclase test results were produced unless a cell population expressing receptors in a homogenous fashion was used. Recombinant alpha2C-ARs in non-neuronal cells were retained inside the cells, and not expressed in the cell membrane, complicating investigation of this receptor subtype. Receptor expression enhancing proteins (REEPs) were found to be neuronalspecific adapter proteins that regulate the processing of the alpha2C-AR, resulting in an increased level of total receptor expression. Current trends call for the use of primary cells endogenously expressing the receptor of interest; therefore, primary human vascular smooth muscle cells (SMC) expressing alpha2-ARs were tested in a functional assay monitoring contractility with a myosin light chain phosphorylation assay. However, these cells were not compatible with this assay due to the loss of differentiation. A rat aortic SMC cell line transfected to express the human alpha2B-AR was adapted for the assay, and it was found that the alpha2-AR agonist, dexmedetomidine, evoked myosin light chain phosphorylation in this model.

Impact of β-2 Thr164Ile and combined β-adrenergic receptor polymorphisms on prognosis in a cohort of heart failure outpatients

Genetic polymorphisms of adrenergic receptors (ARs) have been associated with the development, progression, and prognosis of patients with heart failure (HF), with few data for the Brazilian population. We evaluated the role of the β2-AR Thr164Ile polymorphism at codon 164 on prognosis in a prospective study on 315 adult Brazilian HF patients, predominantly middle-aged Caucasian men in functional class I-II, with severe left ventricular systolic dysfunction. Genomic DNA was extracted from peripheral blood and β2-AR164 genotypes were detected by PCR followed by restriction fragment length analysis. During a median follow-up of 3 years, 95 deaths occurred and 57 (60%) were HF-related. Unexpectedly, Ile164 carriers (N = 12) had no HF-related events (log-rank P value = 0.13). Analysis using genotype combination with β1-AR polymorphisms at codons 49 and 389 identified patients with favorable genotypes (Thr164Ile of β2-AR, Gly49Gly of β1-AR and/or Gly389Gly of β1-AR), who had lower HF-related mortality (P = 0.01). In a Cox proportional hazard model adjusted for other clinical characteristics, having any of the favorable genotypes remained as independent predictor of all-cause (hazard ratio (HR): 0.41, 95%CI: 0.17-0.95) and HF-related mortality (HR: 0.12, 95%CI: 0.02-0.90). These data show that the β2-AR Thr164Ile polymorphism had an impact on prognosis in a Brazilian cohort of HF patients. When combined with common β1-AR polymorphisms, a group of patients with a combination of favorable genotypes could be identified.

Esine ja käsityö. Anu Tuomisen teokset ja työskentely

Anu Tuomisen (s. 1961 Lemi) taideteokset yhdistävät löydettyjä esineitä ja käsityötä. Esineistö on tuttua arkipäivän käyttötavaraa, joka on pääosin hankittu kirpputoreilta tai kerätty luonnosta. Taideteosten ja esineinstallaatioiden lisäksi Tuominen on julkaissut Kuvan sijamuotoja -taidekirjan (1. painos 2001), joka koostuu teosten valokuvista ja kirjallisista teosideoista. Vuonna 2003 Tuominen voitti Ars Fennica -kuvataidepalkinnon. Tarkastelen Anu Tuomisen työskentelytapoja strukturalistisessa viitekehyksessä. Käsittelen esineiden keräilyä ja käsityötä kielenkaltaisina järjestelminä, jotka kykenevät kertomaan jotakin omasta rakentuneisuudestaan. Roland Barthesin kirjoitukset strukturalistisesta toiminnasta ja intertekstuaalisuuden teoria valottavat Tuomisen tapaa kutoa teoksiin merkityksiä ja viittauksia. Käsittelen Anu Tuomisen tekijyyttä tai taiteilijuutta Claude Lévi-Straussin bricoleur- termin avulla. Bricolage on taiteellinen strategia, jossa koostetaan teoksia monimuotoisesta materiaalista – mutta myös tapa yhdistää taide ja elämä. 1980-luvulla bricoleur kytkeytyi Michel de Certeaun kirjoituksissa kekseliääseen ja luovaan kulutukseen. Tuomisen kohdalla bricolage tuo mukanaan myös kysymyksen amatööriyden arvostuk-sesta ja merkityksestä. Tuominen ymmärretään poikkeuksetta käsitetaiteilijaksi. Tuominen käyttää valmisesineitä (readymade), leikkii tekstillä ja kuvalla, pohtii teoksen merkityksen rakentumista ja taiteen metakieltä teoksissaan. Tuomisen teosten keskiössä on prosessuaalisuus, joka korostaa teosten vaatiman työn ja työskentelyn merkitystä. Tuomisen teoksissa käsityö on käsitteellistä, koska sillä prosessoidaan ideoita. Toinen silmiinpistävä piirre Tuomisen teoksissa on tekijyyden kyseenalaistaminen tai monikollisuus. Tutkielmassa luodaan läpileikkaus kanonisoituun antiformalistiseen käsitetaiteeseen Robert Morrisin, Sol LeWittin, Lawrence Weinerin ja Yoko Onon teoksien kautta, mutta se on jäsennetty Tuomisen taiteessa ilmenevien piirteiden kautta.

Mémoire sur la ville d'Alcudia, dans l'isle de Majorque, relatif au plan de cette place

Ancien possesseur : Argenson, Antoine-René de Voyer (1722-1787 ; marquis de Paulmy d')

Mémoire sur la ville d'Yvice qui est dans l'isle du même nom, sur la mer Mediterranée à l'occident de Majorque

Ancien possesseur : Argenson, Antoine-René de Voyer (1722-1787 ; marquis de Paulmy d')

Mémoire sur la ville d'Yvice size dans l'isle de même nom sur la Mediterranée à l'occident de Majorque

Ancien possesseur : Argenson, Antoine-René de Voyer (1722-1787 ; marquis de Paulmy d')

Etat dressé par M. De Rochemore, lieutenant de Roy de la cittadelle de l'isle d'Olleron

Ancien possesseur : Argenson, Antoine-René de Voyer (1722-1787 ; marquis de Paulmy d')

Etat des changemens du cours du Rhin depuis le mois de janvier 1754. jusqu'au mois de may 1755. dans les douze inspections depuis Huningue jusqu'à Lauterbourg

Ancien possesseur : Argenson, Antoine-René de Voyer (1722-1787 ; marquis de Paulmy d')

Fragments de mss.

Poésies (1, 8). — Lettres de Gautier de Mortagne (2), Pierre [Abélard] (6v), frère Eudes (59), Yves de Chartres (98). — Concile de Pont-Audemer en 1279 (10). — Ars dictaminis (20). — Sermons (31). — Homélie de S. Césaire (82v). — Canons (87). — Lettres formées (95v). — Lettre de L., abbé de Pouthières, sur la grammaire (107). — Gerbert sur Porphyre (109). — Questions sur l'Écriture Sainte (117). — Préface de Juvencus (119 et 95). — Traité d'Arnoul de Lisieux contre Girard d'Angoulême (121 et 145). — Libellus Hug. de S. Victore de virtute amoris (137). — Miracle de S. Martin par un clerc d'Utrecht (155).