The effect of the ocean water immersion and UV ageing on the dynamic mechanical properties of the PPS/glass fiber composites

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Seed Dispersal Anachronisms: Rethinking the Fruits Extinct Megafauna Ate

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Envejecimiento y desarrollo en América Latina y el Caribe

Incluye Bibliografía

Analyzing the effect of homogeneous frustration in protein folding

The energy landscape theory has been an invaluable theoretical framework in the understanding of biological processes such as protein folding, oligomerization, and functional transitions. According to the theory, the energy landscape of protein folding is funneled toward the native state, a conformational state that is consistent with the principle of minimal frustration. It has been accepted that real proteins are selected through natural evolution, satisfying the minimum frustration criterion. However, there is evidence that a low degree of frustration accelerates folding. We examined the interplay between topological and energetic protein frustration. We employed a Cα structure-based model for simulations with a controlled nonspecific energetic frustration added to the potential energy function. Thermodynamics and kinetics of a group of 19 proteins are completely characterized as a function of increasing level of energetic frustration. We observed two well-separated groups of proteins: one group where a little frustration enhances folding rates to an optimal value and another where any energetic frustration slows down folding. Protein energetic frustration regimes and their mechanisms are explained by the role of non-native contact interactions in different folding scenarios. These findings strongly correlate with the protein free-energy folding barrier and the absolute contact order parameters. These computational results are corroborated by principal component analysis and partial least square techniques. One simple theoretical model is proposed as a useful tool for experimentalists to predict the limits of improvements in real proteins. © 2013 Wiley Periodicals, Inc.

Jogo e contrajogo: o lúdico no teatro de Antônio José da Silva, O Judeu

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Las finanzas públicas de América Latina en la década de 1980 = Public finances in Latin America in the 1980s

Incluye Bibliografía

Industrial competitiveness policies: the local dimension

Includes bibliography

Educação rural e desenvolvimento local sustentável: a lógica subjacente das relações inter-setoriais

Este estudo tem por objetivo avaliar os modelos de educação gerados nos processos de ocupação da terra nos municípios Juazeiro, Uauá e Valente no Estado da Bahia, tanto nas áreas irrigadas quanto nas de sequeiro, tendo como hipótese norteadora a educação rural como mecanismo disseminador de informações à população e grande mobilizadora das transformações social, econômica, política e cultural das comunidades, na promoção do seu desenvolvimento sustentável. A metodologia da pesquisa tomou por base a análise das redes de relações sociais engendradas no processo, elegendo-se como categorias preferências para a compreensão do fenômeno estudado o capital social, o desenvolvimento local, pedagogias alternativas, currículo escolar e as parcerias intersetoriais, através da ruptura com o antagonismo dos conceitos de indivíduo e de sociedade. Trata-se de pesquisa quanti-qualitativa fundamentada numa abordagem de caráter interativo, onde o discurso emerge como espaço de negociação do sentido e da construção dos sujeitos aprendizes, cujos subsídios somados às evidências quantitativas permitiram o aprofundamento da complexidade dos fenômenos, suas contradições e seu relacionamento com o contexto. A análise dos dados permitiu compreender que a Educação Rural nas áreas pesquisadas vive duas situações: uma, veiculada pelo sistema público de ensino que, salvo algumas experiências pontuais, não atende aos interesses dos povos que habitam e trabalham no campo. Outra, exercitada por Organizações Não Governamentais que valorizando o rural como espaço de vida, forma indivíduos com um repertório de saberes, habilidades e valores capaz de mobilizá-los para uma ação transformadora.

Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A representação da natureza no romance francês do século XIX

Busca-se determinar as características da representação da natureza no romance francês do século XIX, analisando-se Paul et Virginie de Bernardin de Saint-Pierre, Les travailleurs de la mer de Victor Hugo e L’Ève future de Villiers de l’Isle-Adam, por meio da teoria sócio-crítica e do embate com o texto. Na primeira parte, faz-se um retrospecto da representação nas artes e na literatura, destacando-se as concepções de Aristóteles e as do Romantismo. Apresenta-se também um panorama das concepções da natureza no tempo e no espaço e reflete-se sobre as relações do homem com o mundo natural, bem como sobre as teorias a elas referentes no âmbito da história, da pintura e da literatura. Na segunda parte, analisam-se os três romances escolhidos, buscando-se neles os modos e os sentidos da representação literária da natureza. Conclui-se pela existência, nas três obras estudadas, de uma atitude ambígua do homem diante da natureza, que havia sido anteriormente detectada na história. Embora diferenciadas entre si, sob alguns aspectos, as representações da natureza no corpus estudado demonstram o registro, no universo literário, de marcas do contexto histórico, político e social em que os três romances foram escritos, publicados e usufruidos.

Validação das unidades arbitrárias de teor de clorofilas obtido em folhas intactas de seringueira

The determination of foliar chlorophyll content is a characteristic that interests ecophysiologists and rural producers. With consideration for practical uses as well as scientific publications, our present work aims to establish equations, for rubber tree leaves, that can convert arbitrary units of expressing chlorophyll content to the international system. Chlorophyll a (Chla), chlorophyll b (Chlb), and total chlorophyll (Chltot) were obtained from intact leaves using a portable chlorophyll detecting instrument. Leaves from different positions on the plant, at various stages of maturity, and representing a large spectrum of pigment concentrations, were collected and analyzed in the field using the Clorofil OG Falkner ® instrument, through four evaluations in forty-five medium leaflets. At the laboratory, leaflets underwent a process of pigment extraction. They were incubated in a water-bath with dimethylsulfoxide (DMSO), dosed in molecular absorption spectrophotometer, and converted into pigment content per unit of fresh weight using conventional equations. The data were evaluated according to the Pearson correlation coefficient and tested with different regression models. For all variables, the linear fit is the most adequate, with correlation coefficients (r) 0.74 for Chlb and 0.88 for Chla and Chltott.

Validação das unidades arbitrárias do teor de clorofilas obtido em folhas intactas de seringueira

The determination of foliar chlorophyll content is a characteristic that interests ecophysiologists and rural producers. With consideration for practical uses as well as scientific publications, our present work aims to establish equations, for rubber tree leaves, that can convert arbitrary units of expressing chlorophyll content to the international system. Chlorophyll a (Chla), chlorophyll b (Chlb), and total chlorophyll (Chltot) were obtained from intact leaves using a portable chlorophyll detecting instrument. Leaves from different positions on the plant, at various stages of maturity, and representing a large spectrum of pigment concentrations, were collected and analyzed in the field using the Clorofil OG Falkner ® instrument, through four evaluations in forty-five medium leaflets. At the laboratory, leaflets underwent a process of pigment extraction. They were incubated in a water-bath with dimethylsulfoxide (DMSO), dosed in molecular absorption spectrophotometer, and converted into pigment content per unit of fresh weight using conventional equations. The data were evaluated according to the Pearson correlation coefficient and tested with different regression models. For all variables, the linear fit is the most adequate, with correlation coefficients (r) 0.74 for Chlb and 0.88 for Chla and Chltott .

Segmentation of Cell Nuclei Regions in Epithelium of Prostate Glands

This paper proposes a method for segmentation of cell nuclei regions in epithelium of prostate glands. This structure provides information to diagnosis and prognosis of prostate cancer. In the initial step, the contrast stretching technique was applied in image in order to improve the contrast between regions of interest and other regions. After, the global thresholding technique was applied and the value of threshold was defined empirically. Finally, the false positive regions were removed using the connected components technique. The performance of the proposed method was compared with the Otsu technique and statistical measures of accuracy were calculated based on reference images (gold standard). The result of the mean value of accuracy of proposed method was 93% ± 0.07.

Anchieta, o Orfeu Brasílico: a prática do gênero epidíctico em três epigramas

Pós-graduação em Letras - FCLAS