On the Velocity and Acceleration Estimation from Discrete Time-Position Sensors

In this work is addressed the topic of estimation of velocity and acceleration from digital position data. It is presented a review of several classic methods and implemented with real position data from a low cost digital sensor of a hydraulic linear actuator. The results are analyzed and compared. It is shown that static methods have a limited bandwidth application, and that the performance of some methods may be enhanced by adapting its parameters according to the current state.

Contribuição à história dos batistas Sul-Mato-grossenses a partir da memória coletiva Um Recorte Biográfico da missionária Ann Mae Louise Wollerman

Esta pesquisa parte do interesse de análise da contribuição da missionária Ana Wollerman para o crescimento da denominação batista no sul de Mato Grosso e Mato Grosso do Sul, no período compreendido entre os anos de 1948 a 1978. A memória religiosa e autobiográfica da missionária apresenta experiências com o sagrado que marcam divisores de fases e temporalidades no seu recorte biográfico e que influenciam decisivamente na postura ministerial adotada. As entrevistas com algumas pessoas que participaram das comunidades afetivas existentes e os registros nas atas lidas constatam em grande parte os dados coletados pela memória. Ana Wollerman, filha de descendentes de alemães nos E.U.A., graduou-se em Artes e pósgraduou- se em Educação Religiosa. Veio para o Brasil inicialmente como missionária sem depender do sustento financeiro de uma Junta Missionária, fundou diversas escolas de ensino primário, trabalhou na implantação de diversas igrejas e dedicou grande parte de seus esforços no ensino ministerial. Foi responsável pela ajuda financeira no sustento de mais de uma dezena de jovens nos Seminários de Curitiba-PR, no IBER-RJ e no Seminário do Sul-RJ. Contribuiu também para que fossem destinadas grandes ofertas para a construção do Seminário Teológico Batista em Dourados. O trabalho procura seguir uma metodologia ainda em construção no que se refere à memória religiosa e utiliza o referencial teórico de Maurice Halbwachs para apresentar as memórias individuais e construção da memória coletiva, bem como tem apoio no próprio Halbwachs ao trabalhar a leitura da formação das comunidades afetivas. Aliado a estas questões se presta como um primeiro tratado sobre a historiografia da denominação batista em Mato Grosso e Mato Grosso do Sul, reunindo aspectos da sua gênese e do seu desenvolvimento. O resgate e a valorização da memória do sujeito-objeto em questão, ainda em vida constitui também no reconhecimento que a academia pode prestar às pessoas e às comunidades que se dedicam à construção de um mundo melhor.(AU)

Cu-NirK from Haloferax mediterranei as an example of metalloprotein maturation and exportation via Tat system

The green Cu-NirK from Haloferax mediterranei (Cu-NirK) has been expressed, refolded and retrieved as a trimeric enzyme using an expression method developed for halophilic Archaea. This method utilizes Haloferax volcanii as a halophilic host and an expression vector with a constitutive and strong promoter. The enzymatic activity of recombinant Cu-NirK was detected in both cellular fractions (cytoplasmic fraction and membranes) and in the culture media. The characterization of the enzyme isolated from the cytoplasmic fraction as well as the culture media revealed important differences in the primary structure of both forms indicating that Hfx. mediterranei could carry out a maturation and exportation process within the cell before the protein is exported to the S-layer. Several conserved signals found in Cu-NirK from Hfx. mediterranei sequence indicate that these processes are closely related to the Tat system. Furthermore, the N-terminal sequence of the two Cu-NirK subunits constituting different isoforms revealed that translation of this protein could begin at two different points, identifying two possible start codons. The hypothesis proposed in this work for halophilic Cu-NirK processing and exportation via the Tat system represents the first approximation of this mechanism in the Halobacteriaceae family and in Prokarya in general.

Regulation of ammonium assimilation in Haloferax mediterranei: Interaction between glutamine synthetase and two GlnK proteins

GlnK proteins belong to the PII superfamily of signal transduction proteins and are involved in the regulation of nitrogen metabolism. These proteins are normally encoded in an operon together with the structural gene for the ammonium transporter AmtB. Haloferax mediterranei possesses two genes encoding for GlnK, specifically, glnK1 and glnK2. The present study marks the first investigation of PII proteins in haloarchaea, and provides evidence for the direct interaction between glutamine synthetase and both GlnK1 and GlnK2. Complex formation between glutamine synthetase and the two GlnK proteins is demonstrated with pure recombinant protein samples using in vitro activity assays, gel filtration chromatography and western blotting. This protein–protein interaction increases glutamine synthetase activity in the presence of 2-oxoglutarate. Separate experiments that were carried out with GlnK1 and GlnK2 produced equivalent results.

Algorithm for Correcting the Keratometric Error in the Estimation of the Corneal Power in Eyes With Previous Myopic Laser Refractive Surgery

Purpose: To calculate theoretically the errors in the estimation of corneal power when using the keratometric index (nk) in eyes that underwent laser refractive surgery for the correction of myopia and to define and validate clinically an algorithm for minimizing such errors. Methods: Differences between corneal power estimation by using the classical nk and by using the Gaussian equation in eyes that underwent laser myopic refractive surgery were simulated and evaluated theoretically. Additionally, an adjusted keratometric index (nkadj) model dependent on r1c was developed for minimizing these differences. The model was validated clinically by retrospectively using the data from 32 myopic eyes [range, −1.00 to −6.00 diopters (D)] that had undergone laser in situ keratomileusis using a solid-state laser platform. The agreement between Gaussian (PGaussc) and adjusted keratometric (Pkadj) corneal powers in such eyes was evaluated. Results: It was found that overestimations of corneal power up to 3.5 D were possible for nk = 1.3375 according to our simulations. The nk value to avoid the keratometric error ranged between 1.2984 and 1.3297. The following nkadj models were obtained: nkadj= −0.0064286r1c + 1.37688 (Gullstrand eye model) and nkadj = −0.0063804r1c + 1.37806 (Le Grand). The mean difference between Pkadj and PGaussc was 0.00 D, with limits of agreement of −0.45 and +0.46 D. This difference correlated significantly with the posterior corneal radius (r = −0.94, P < 0.01). Conclusions: The use of a single nk for estimating the corneal power in eyes that underwent a laser myopic refractive surgery can lead to significant errors. These errors can be minimized by using a variable nk dependent on r1c.

al- Durr al-munaẓẓam fī sharḥ al-ism al-aʻẓam : manuscript, undated

Written in one column, 20 lines per page, in black rubricated in red. Text framed within double red lines and each line framed within a red line.

Angelo Poliziano.

Bibliographical references in "Note" (p. [147]-158)

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Contiene con paginación independiente: Physica comentatio de risu [et] lacrymis.

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Mode of access: Internet.

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Mode of access: Internet.

Tertius tomus operum Angeli Politiani, eiusdem praelectiones, orationes, [et] Epigra[m]mata complectens, quorum versa pagella reperies.

CCBE S. XVI

Histoire du connêtable de Lune, favori de Jean II, Roi de Castille & de Leon.

Mode of access: Internet.