Representational difference analysis:Critical appraisal and method development for the identification of unique DNA sequences from prokaryotes

Representational difference analysis (RDA) has great potential for preferential amplification of unique but uncharacterised DNA sequences present in one source such as a whole genome, but absent from a related genome or other complex population of sequences. While a few examples of its successful exploitation have been published, the method has not been well dissected and robust, detailed published protocols are lacking. Here we examine the method in detail, suggest improvements and provide a protocol that has yielded key unique sequences from a pathogenic bacterial genome. © 2003 Elsevier Science B.V. All rights reserved.

Organization and Security of the Audio and Video Archive for Unique Bulgarian Bells

AMS Subj. Classification: H.3.7 Digital Libraries, K.6.5 Security and Protection

Warehousing and OLAP Analysis of Data About Unique Bulgarian Bells

Тихомир Трифонов, Цветанка Георгиева-Трифонова - В настоящата статия е представена системата bgBell/OLAP за складиране и онлайн аналитична обработка на данни за уникални български камбани. Реализираната система предоставя възможност за извеждане на обобщени справки и анализиране на различни характеристики на камбаните, за да се извлече предварително неизвестна и потенциално полезна информация.

Building OWL Ontology of Unique Bulgarian Bells Using Protégé Platform

We describe an ontological representation of data in an archive containing detailed description of church bells. As an object of cultural heritage the bell has general properties such as geometric dimensions, weight, sound of each of the bells, the pitch of the tone as well as acoustical diagrams obtained using contemporary equipment. We use Protégé platform in order to define basic ontological objects and relations between them.

Weak Ergodicity of Mt /Mt /N /N + R Queue

2000 Mathematics Subject Classification: 60J27, 60K25.

Some Inequalities of the Uniform Ergodicity and Strong Stability of Homogeneous Markov Chains

2000 Mathematics Subject Classification: 60J45, 60K25

Unique temperature dependence of selectively liquid-crystal-filled photonic crystal fibers

We demonstrate a unique temperature-dependent characteristic of the selectively liquid-crystal-filled photonic crystal fiber, which is realized by a selectively infiltrating liquid crystal into a single air hole located at the second ring near the core of the PCF. Three-resonance dips are observed in the transmission spectrum. Theoretical and experimental investigations reveal that the three-resonance dips all result from the coupling between the LP01 core mode and the rod modes, i.e., LP03 and LP51. Then, we find that the dip shift induced by temperature shows good agreements with the thermo-optic performance of the LC employed. Furthermore, the dips shift greatly with changes in temperature, providing a method to achieve temperature measurement in such a compact structure.

Phosphorus Biogeochemistry and the Impact of Phosphorus Enrichment: Why Is the Everglades so Unique?

The Florida Everglades is extremely oligotrophic and sensitive to small increases in phosphorus (P) concentrations. P enrichment is one of the dominant anthropogenic impacts on the ecosystem and is therefore a main focus of restoration efforts. In this review, we synthesize research on P biogeochemistry and the impact of P enrichment on ecosystem structure and function in the Florida Everglades. There are clear patterns of increased P concentrations and altered structure and processes along nutrient-enrichment gradients in the water, periphyton, soils, macrophytes, and consumers. Periphyton, an assemblage of algae, bacteria, and associated microfauna, is abundant and has a large influence on phosphorus cycling in the Everglades. The oligotrophic Everglades is P-starved, has lower P concentrations and higher nitrogen–phosphorus (N:P) ratios, and has oxidized to only slightly reduced soil profiles compared to other freshwater wetland ecosystems. Possible general causes and indications of P limitation in the Everglades and other wetlands include geology, hydrology, and dominance of oxidative microbial nutrient cycling. The Everglades may be unique with respect to P biogeochemistry because of the multiple causes of P limitation and the resulting high degree of limitation.

Quadratic and Symmetric Bilinear Forms on Modules with Unique Base Over a Semiring

27 pages

A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway

Acknowledgements This work was supported by funding of the Academy of Finland (259505), Helsinki University Research grant (490085) and ESCMID grant (4720572) to D.P.F., University of Pittsburgh Central Research Development Fund to X.L., Technology Strategy Board grant (131181) to W.H., M.J. and J.H.N. National Programme of Sustainability I of the Ministry of Education of the Czech Republic I grant (LO1416) to T.G.

Quadratic and symmetric bilinear forms on modules with unique base over a semiring

Peer reviewed

A Longitudinal Investigation of Infant Gesture Use and Parent Speech: Unique and Dynamic Influences on Infant Vocabulary Acquisition

How do infants learn word meanings? Research has established the impact of both parent and child behaviors on vocabulary development, however the processes and mechanisms underlying these relationships are still not fully understood. Much existing literature focuses on direct paths to word learning, demonstrating that parent speech and child gesture use are powerful predictors of later vocabulary. However, an additional body of research indicates that these relationships don’t always replicate, particularly when assessed in different populations, contexts, or developmental periods.

The current study examines the relationships between infant gesture, parent speech, and infant vocabulary over the course of the second year (10-22 months of age). Through the use of detailed coding of dyadic mother-child play interactions and a combination of quantitative and qualitative data analytic methods, the process of communicative development was explored. Findings reveal non-linear patterns of growth in both parent speech content and child gesture use. Analyses of contingency in dyadic interactions reveal that children are active contributors to communicative engagement through their use of gestures, shaping the type of input they receive from parents, which in turn influences child vocabulary acquisition. Recommendations for future studies and the use of nuanced methodologies to assess changes in the dynamic system of dyadic communication are discussed.