Waddlia chondrophila induces systemic infection, organ pathology, and elicits Th1-associated humoral immunity in a murine model of genital infection

Waddlia chondrophila is a known bovine abortigenic Chlamydia-related bacterium that has been associated with adverse pregnancy outcomes in human. However, there is a lack of knowledge regarding how W. chondrophila infection spreads, its ability to elicit an immune response and induce pathology. A murine model of genital infection was developed to investigate the pathogenicity and immune response associated with a W. chondrophila infection. Genital inoculation of the bacterial agent resulted in a dose-dependent infection that spread to lumbar lymph nodes and successively to spleen and liver. Bacterial-induced pathology peaked on day 14, characterized by leukocyte infiltration (uterine horn, liver, and spleen), necrosis (liver) and extramedullary hematopoiesis (spleen). Immunohistochemistry demonstrated the presence of a large number of W. chondrophila in the spleen on day 14. Robust IgG titers were detected by day 14 and remained high until day 52. IgG isotypes consisted of high IgG2a, moderate IgG3 and no detectable IgG1, indicating a Th1-associated immune response. This study provides the first evidence that W. chondrophila genital infection is capable of inducing a systemic infection that spreads to major organs, induces uterus, spleen, and liver pathology and elicits a Th1-skewed humoral response. This new animal model will help our understanding of the mechanisms related to intracellular bacteria-induced miscarriages, the most frequent complication of pregnancy that affects one in four women.

The comprehensiveness of the ESHRE/ESGE classification of female genital tract congenital anomalies: a systematic review of cases not classified by the AFS system.

STUDY QUESTION How comprehensive is the recently published European Society of Human Reproduction and Embryology (ESHRE)/European Society for Gynaecological Endoscopy (ESGE) classification system of female genital anomalies? SUMMARY ANSWER The ESHRE/ESGE classification provides a comprehensive description and categorization of almost all of the currently known anomalies that could not be classified properly with the American Fertility Society (AFS) system. WHAT IS KNOWN ALREADY Until now, the more accepted classification system, namely that of the AFS, is associated with serious limitations in effective categorization of female genital anomalies. Many cases published in the literature could not be properly classified using the AFS system, yet a clear and accurate classification is a prerequisite for treatment. STUDY DESIGN, SIZE AND DURATION The CONUTA (CONgenital UTerine Anomalies) ESHRE/ESGE group conducted a systematic review of the literature to examine if those types of anomalies that could not be properly classified with the AFS system could be effectively classified with the use of the new ESHRE/ESGE system. An electronic literature search through Medline, Embase and Cochrane library was carried out from January 1988 to January 2014. Three participants independently screened, selected articles of potential interest and finally extracted data from all the included studies. Any disagreement was discussed and resolved after consultation with a fourth reviewer and the results were assessed independently and approved by all members of the CONUTA group. PARTICIPANTS/MATERIALS, SETTING, METHODS Among the 143 articles assessed in detail, 120 were finally selected reporting 140 cases that could not properly fit into a specific class of the AFS system. Those 140 cases were clustered in 39 different types of anomalies. MAIN RESULTS AND THE ROLE OF CHANCE The congenital anomaly involved a single organ in 12 (30.8%) out of the 39 types of anomalies, while multiple organs and/or segments of Müllerian ducts (complex anomaly) were involved in 27 (69.2%) types. Uterus was the organ most frequently involved (30/39: 76.9%), followed by cervix (26/39: 66.7%) and vagina (23/39: 59%). In all 39 types, the ESHRE/ESGE classification system provided a comprehensive description of each single or complex anomaly. A precise categorization was reached in 38 out of 39 types studied. Only one case of a bizarre uterine anomaly, with no clear embryological defect, could not be categorized and thus was placed in Class 6 (un-classified) of the ESHRE/ESGE system. LIMITATIONS, REASONS FOR CAUTION The review of the literature was thorough but we cannot rule out the possibility that other defects exist which will also require testing in the new ESHRE/ESGE system. These anomalies, however, must be rare. WIDER IMPLICATIONS OF THE FINDINGS The comprehensiveness of the ESHRE/ESGE classification adds objective scientific validity to its use. This may, therefore, promote its further dissemination and acceptance, which will have a positive outcome in clinical care and research. STUDY FUNDING/COMPETING INTERESTS None.

Antibiotic Susceptibility and Sequence Type Distribution of Ureaplasma Species Isolated from Genital Samples in Switzerland.

Antibiotic resistance in Ureaplasma urealyticum/Ureaplasma parvum and Mycoplasma hominis is an issue of increasing importance. However, data regarding the susceptibility and, more importantly, the clonality of these organisms are limited. We analyzed 140 genital samples obtained in Bern, Switzerland, in 2014. Identification and antimicrobial susceptibility tests were performed by using the Mycoplasma IST 2 kit and sequencing of 16S rRNA genes. MICs for ciprofloxacin and azithromycin were obtained in broth microdilution assays. Clonality was analyzed with PCR-based subtyping and multilocus sequence typing (MLST), whereas quinolone resistance and macrolide resistance were studied by sequencing gyrA, gyrB, parC, and parE genes, as well as 23S rRNA genes and genes encoding L4/L22 ribosomal proteins. A total of 103 samples were confirmed as positive for U. urealyticum/U. parvum, whereas 21 were positive for both U. urealyticum/U. parvum and M. hominis. According to the IST 2 kit, the rates of nonsusceptibility were highest for ciprofloxacin (19.4%) and ofloxacin (9.7%), whereas low rates were observed for clarithromycin (4.9%), erythromycin (1.9%), and azithromycin (1%). However, inconsistent results between microdilution and IST 2 kit assays were recorded. Various sequence types (STs) observed previously in China (ST1, ST2, ST4, ST9, ST22, and ST47), as well as eight novel lineages, were detected. Only some quinolone-resistant isolates had amino acid substitutions in ParC (Ser83Leu in U. parvum of serovar 6) and ParE (Val417Thr in U. parvum of serovar 1 and the novel Thr417Val substitution in U. urealyticum). Isolates with mutations in 23S rRNA or substitutions in L4/L22 were not detected. This is the first study analyzing the susceptibility of U. urealyticum/U. parvum isolates in Switzerland and the clonality outside China. Resistance rates were low compared to those in other countries. We hypothesize that some hyperepidemic STs spread worldwide via sexual intercourse. Large combined microbiological and clinical studies should address this important issue.

Optimal frequency of Imiquimod (Aldara(RTM)) 5% cream treatment of external genital warts in immunocompetent adults. Systematic review and meta-analysis

Genital warts are a sexually transmitted disease with high prevalence in the U.S. Imiquimod 5% cream is a self-applied treatment, prescribed three-times weekly, at bedtime, for 16 weeks. The post-marketing research addressed questions of imiquimod dosing frequency. MEDLINE, Embase, and the Cochrane Library were searched for randomized trials on efficacy and safety of imiquimod 5% cream with either three-times weekly or once-daily regimens to systemically review treatment options. Efficacy was evaluated by completely cleared warts at the end of treatment, and safety - by frequency of adverse events and at least one rest period taken from treatment. Six studies were selected for the analysis, including circumcised men, uncircumcised men, and women. The once-daily compared to three-times weekly regimen did not improve the efficacy, but resulted in increased incidence of local skin reactions and events, when at least one rest period was taken from treatment. The optimal regimen is three-times weekly.^

The influence of HIV/AIDS on the association between genital human papillomavirus and cervical cancer in HIV/AIDS infected women: A literature review

Background: With over 440 million cases of infections worldwide, genital HPV is the most frequent sexually transmitted infection. There are several types including high risk types 16, 18, 58 and 70 among others, which are known to cause cervical cell abnormality and if persistent, can lead to cervical cancer which globally, claims 288,000 lives annually. 33.4 million people worldwide are currently living with HIV/AIDS, with 22.4 million in sub-Saharan Africa where 70% of the female population living with HIV/AIDS is also found. Similar risk factors for HPV, cervical cancer and HIV/AIDS include early age at sexual debut, multiple sexual partners, infrequent condom use, history of STI and immune-suppression. ^ Objectives: To describe the role of HPV in cervical cancer development, to describe the influence of HIV/AIDS on HPV and in the development of cervical cancer and to describe the importance of preventive measures such as screening. ^ Methods: This is a literature review where data were analyzed qualitatively and a descriptive narrative style used to evaluate and present the information. The data came from searches using Pub Med, Cochrane Library, EBSCO Medline databases as well as websites such as the CDC and WHO. Articles selected were published in English over the last 10 years. Keywords used included: 'HPV, cervical cancer and HIV', 'HIV and HPV', 'HPV and cervical cancer', 'HPV infection', 'HPV vaccine', 'genital HPV', 'HIV and cervical cancer', 'prevalence of HIV and cervical cancer' and 'prevalence of cervical cancer'. ^ Results: Women with HIV/AIDS have multiple HPV types, persistent infection, are more likely to present with cervical neoplasia and are at higher risk for cervical cancer. Research also shows that HIV could affect the transmissibility of HPV and that HPV itself could also increase the susceptibility to HIV acquisition. ^ Conclusion: HIV, genital HPV and cervical cancer are all preventable. Need to emphasize programs that aim to increase HIV/AIDS, HPV and cervical cancer awareness. Stress importance of behavior modification such as frequent use of condoms, decreased sexual partners and delayed first intercourse. Facilitate programs for screening and treating HPV, male circumcision, effective management of HAART and HPV vaccination.^

Memoria, historia y ficción en la escritura femenina del siglo XIX : la ciudad heroica de Rosario Puebla de Godoy

Fil: Hintze, Gloria. Universidad Nacional de Cuyo. Facultad de Filosofía y Letras

Ciudadanía femenina en El Salvador : Prudencia Ayala

El relato de la historia mundial, y de la historia de América Latina, ha sido escrito mayoritaria- mente por hombres. Por esa razón, este trabajo da cuenta de la participación y contribución del género masculino, con la excepción de contadas mujeres en el Salvador. Si bien es cierto que muchos campos estuvieron vedados a las mujeres por siglos, éstas no cesaron de defender la igualdad de derechos, constituyéndose en verdaderas protagonistas de nuestra historia en el campo de la política y la ciudadanía femenina. Por ello centramos el foco en el proceso de lucha de las mujeres salvadoreñas para ejercer este derecho, a través de la figura Prudencia Ayala, una mujer indígena y pobre, candidata a la presidencia en 1930.

Las representaciones de la sexualidad femenina en el campo de las políticas públicas

El tema de investigación de esta tesis consiste en las representaciones de la sexualidad femenina inscriptas en las políticas públicas implementadas por los Estados después de asumir los compromisos correspondientes en las distintas conferencias de las Naciones Unidas. Nos hemos centrado en la descripción y el análisis de las políticas referidas a salud y derechos sexuales y reproductivos en la provincia de Mendoza, procurando realizar un seguimiento histórico que abarca desde el momento de la formación de los organismos encargados de la ejecución de las políticas públicas hacia mujeres hasta el presente. La investigación, elaborada desde un punto de vista teórico de género, implica considerar tanto los procesos que llevaron a los Estados a intervenir en relación con la vida de las mujeres; como la perspectiva de las actoras y los actores sociales y estatales. Históricamente, las mujeres han sido las encargadas del bienestar familiar y sus roles se han delimitado estrechamente vinculados a sus funciones dentro de la familia. Tanto es así, que las mujeres sólo eran incluidas en la planificación social como parte de la unidad familiar y/o como pertenecientes a grupos vulnerables y carenciados o bien en relación con políticas natalistas o antinatalistas, pero nunca atendiendo a las problemáticas resultantes de su condición específica de mujeres. Esta situación comienza a modificarse en las décadas de 1960 y 1970, cuando los organismos internacionales empezaron a intervenir con relación al papel de las mujeres en el desarrollo y a diseñar políticas específicas que las tuvieran como objeto, considerándolas en sus roles de madres, esposas y como agentes económicos al interior de la familia. Simultáneamente emergía en occidente la llamada segunda ola del feminismo, que cuestionaba las jerarquías sexuales existentes e intentaba instalar en la sociedad sus demandas presionando sobre los gobiernos por el reconocimiento de sus derechos.

El sistema genital masculino y el control androtrófico en un ampulárido

Fil: Gamarra Luques, Carlos . Universidad Nacional de Cuyo. Facultad de Ciencias Médicas

“Idolatrado ancón" : identificación con la nación panameña desde la subjetividad femenina de los versos de Amelia Denis de Icaza

Ciento diez años más tarde de la separación de Panamá de Colombia y con el advenimiento del centenario de la apertura del Canal en 2014 es posible observar que la obra de Amelia Denis de Icaza muestra cómo la poeta romántica panameña, desde una perspectiva femenina, inaugura en 1906 la articulación de la fisura como rasgo característico de la identidad nacional de Panamá. Denis de Icaza inicia el lamento y la crítica por la presencia norteamericana en el Istmo con su poema “Al Cerro Ancón". En sus versos, la autora expresa su desagrado por la nueva realidad de ocupación territorial por fuerzas imperialistas y al mismo tiempo enciende el deseo por recuperar lo perdido, representándolo todo en el iconográfico accidente geográfico del Cerro Ancón. Esta nueva presencia extranjera y las consecuencias que se desprenden de tal ocupación trascienden el imaginario panameño a lo largo del siglo XX y más allá. Denis de Icaza, desde su espacio privado y con su voz intimista problematiza la situación canalera dentro del imaginario panameño, y se convierte en líder de la travesía que enrumbará al discurso istmeño en torno a la búsqueda y promoción por la soberanía en la totalidad del territorio nacional.

Ley 25.673/02: Créase el Programa Nacional de Salud Sexual y Procreación Responsable, en el ámbito del Ministerio de Salud

Serán objetivos de este programa: a) Alcanzar para la población el nivel más elevado de salud sexual y procreación responsable con el fin de que pueda adoptar decisiones libres de discriminación, coacciones o violencia; b) Disminuir la morbimortalidad materno-infantil; c) Prevenir embarazos no deseados; d) Promover la salud sexual de los adolescentes; e) Contribuir a la prevención y detección precoz de enfermedades de transmisión sexual, de vih/sida y patologías genital y mamarias; f) Garantizar a toda la población el acceso a la información, orientación, métodos y prestaciones de servicios referidos a la salud sexual y procreación responsable; g) Potenciar la participación femenina en la toma de decisiones relativas a su salud sexual y procreación responsable.

División sexual del trabajo no remunerado y participación política femenina : Transformaciones necesarias para la igualdad de género

En el trabajo me propongo reflexionar sobre las barreras que impiden a las mujeres acceder a su derecho de participación política, en función de las relaciones de inequidad dentro de las familias. Abordaré cómo las características de dicha institución y la división sexual del trabajo que en ella se establece, tal como está estructurada actualmente en la sociedad argentina, influye en la posibilidad de ejercicio de la ciudadanía femenina. En este contexto, es sumamente relevante cuestionar la desigualdad en el trabajo de cuidado no remunerado como una restricción a la ciudadanía ampliada de las mujeres, y en particular como una importante barrera para desempeñar cargos públicos representativos. En función de esto presentaré y analizaré los datos de la Encuesta sobre Trabajo No Remunerado y Uso del Tiempo, realizada en Argentina en el año 2013, junto con los datos sobre representación de la mujer en los órganos políticos del mismo país