Possessed by the beast: subjectivity and agency in pictures in the dark and foxspell

The first book to assess critically mystery in children's literature, this collection charts a development from religious mystery through rationally solved detective fictions to insoluble supernatural and horror mysteries. Written by internationally recognised scholars in the field, these thirteen original essays offer challenging and innovative readings of both classic and popular mysteries for children. This volume will be essential and stimulating reading for anyone with an interest in children's literature or in mystery fiction.

Characterization of AFN1, a Gene Associated with Cereal Grain Germination

The AFN1 gene is transiently expressed in germinating oat grains. As AFN1 is not expressed in dormant oat grains during imbibition, we hypothesize that AFN1 may be involved in stimulating the germination process. Sequence analysis of an AFN1 cDNA clone indicates that the AFN1 polypeptide is similar to a previously identified abscisic acid (ABA) glucosyl transferase. This suggests that AFN1 may be acting to glucosylate ABA, thereby inactivating it. As the hormone ABA is known to inhibit germination, ABA glucosylation/inactivation could lead to germination in grains expressing AFN1. To test this hypothesis, we have constructed an expression plasmid that encodes an MBP::AFN1 (maltose binding protein) fusion protein. E. coli cells carrying the expression plasmid were found to produce the MBP::AFN1 fusion protein as a substantial fraction of total protein. We are currently in the process of purifying the MBP::AFN1 fusion protein by affinity chromatography, so that it can be assayed for ABA glucosyl transferase activity. We also wish to test the effect of AFN1 gene expression during grain imbibition on the germination behavior of the grains. To this end, we have constructed plasmids for the overexpression and RNAi-based suppression of AFN1 in transgenic plants. These plasmids have been introduced into oat cells by particle bombardment and we are in the process of regenerating transgenic plants for study.

Upside Down World -- the Opposition Between Light and Dark in Bulgakov's Master and Margarita

A look at the role that symbolism plays in the novel. In this case, as it is in many other great novels, we see that symbolism is used to enhance the mood and the atmosphere of the novel rather than adding anything of importance to the plot.

All that is dark can become white: the rules of the game in Bend it Like Beckham

The “light” and uplifting film, Bend it Like Beckham (2002), is deconstructed to expose its passive ideologies that equate physical darkness with regressive cultural and social outlooks and practices. While Bend it Like Beckham constructs itself as a modern fairy tale of a girl achieving her dream of athletic opportunity and success (with a nice side-dish of romance), the film’s privileging of whiteness is both a cultural and a gendered norm that must be desired and achieved before that dream may come true.

Dark light

Daniel Armstrong takes astronomical images of the southern sky. From these images each star is individually selected and mapped into grid like alignments and symmetrical shapes. The images present an optical play between structure and mapping.

Environmental influences on akinete germination and development in Nodularia spumigena (Cyanobacteriaceae), isolated from the Gippsland Lakes, Victoria, Australia

This study was carried out to investigate the genesis of N. spumigena blooms by specifically studying the effects of environmental variables (salinity, nitrogen, phosphorus and light) on the germination of N. spumigena akinetes. Optimal conditions for maximum germination and germling growth were determined by exposing akinetes to a range of salinities and nutrient (nitrogen and phosphorus) concentrations under two different irradiances. At pre-determined time periods, treatments were sampled and the percent germination and length of germlings assessed. The results indicated that akinete germination and germling growth were optimal at salinities from 5 to 25 and significantly reduced outside this range. A positive correlation in germination was observed with increasing nutrient (phosphorus and nitrate) concentration. Similarly, germling growth increased with increasing concentrations of both nutrients. Irradiance significantly influenced both germination and growth during salinity experiments, whereas in nutrient addition experiments, irradiance had no effect on germination; however, growth was significantly influenced during phosphorus addition experiments. Consequently, salinity and light appeared to be most critical in the germination process for N. spumigena akinetes, with phosphorus most important for germling growth. The study showed that N. spumigena may be able to germinate under environmental conditions outside its optimal range, but the growth of the germling is significantly reduced, which in turn suggests that its ability to form a bloom outside its optimal environmental conditions would also be greatly reduced.

Trainers and transformation : facilitating the 'dark side' of vocational learning

'Transformative learning' is a term used by Mezirow (1991) and his followers to designate a specifically 'adult' kind of learning that involves shifts in how learners view the world and themselves. New research into learning in VET suggests that in some subject areas transformative learning may play more than an incidental role. Among the implications of this finding is that the trainer's practice may be more important in VET than it has been the custom to acknowledge. When transformative learning systematically contributes to VET, the trainer becomes a co-constructor of competence rather than a transmitter of skills and knowledge.

This paper reports on this new research and reflects on the role of the trainer in the process of VET-oriented transformative learning. Results indicate that some trainers of youth workers develop a practice that responds to the contours and dangers of transformative learning without necessarily being aware of the body of knowledge that has built up around this type of learning. The paper suggests that in some VET sectors, trainers and RTO's could enhance their work by taking stock of transformative learning research and theory.