Das Grosse Zumahlen Geistliche Elend Des Jüdischen Volckes Bey Gelegenheit Einer Juden-Tauffe : Aus 2. Cor. III, 15.16.17 In der Hospital-Kirche d. 29. Aug. 1743 Der Zahlreichen Gemeinde des Herrn vorgetragen, Und heraus gegeben Von Gottfried Thomas Zeitmann, Evangel. Prediger in Franckfurt am Mayn

Hss. auf Titelbl. Sign. aus Freimann-Kat.:Jud. Ff. 599

Human bone chips release of sclerostin and FGF-23 into the culture medium: an in vitro pilot study

Abstract OBJECTIVE: Signaling molecules derived from osteocytes have been proposed as a mechanism by which autografts contribute to bone regeneration. However, there have been no studies that determined the role of osteocytes in bone grafts. MATERIAL AND METHOD: Herein, it was examined whether bone chips and demineralized bone matrix release sclerostin and FGF-23, both of which are highly expressed by osteocytes. RESULTS: Bone grafts from seven donors were placed in culture medium. Immunoassay showed that bone chips released sclerostin (median 1.0 ng/ml) and FGF-23 (median 9.8 relative units/ml) within the first day, with declining levels overtime. Demineralized bone matrix also released detectable amounts of sclerostin into culture medium, while FGF-23 remained close to the detection limit. In vitro expanded isolated bone cells failed to release detectable amounts of sclerostin and FGF-23. CONCLUSION: These results suggest that autografts but also demineralized bone matrix can release signaling molecules that are characteristically produced by osteocytes. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. KEYWORDS: FGF-23; autologous bone; bone grafts; demineralized bone matrix; osteocytes; sclerostin

Antiseptic solutions modulate the paracrine-like activity of bone chips: differential impact of chlorhexidine and sodium hypochlorite

AIM: Chemical decontamination increases the availability of bone grafts; however, it is unclear whether antiseptic processing changes the biological activity of bone. MATERIALS AND METHODS: Bone chips were incubated with 4 different antiseptic solutions including (1) povidone-iodine (0.5%), (2) chlorhexidine diguluconate (0.2%), (3) hydrogen peroxide (1%) and (4) sodium hypochlorite (0.25%). After 10 minutes of incubation, changes in the capacity of the bone-conditioned medium to modulate gene expression of gingival fibroblasts was investigated. RESULTS: Conditioned medium obtained from freshly prepared bone chips increased the expression of TGF-β target genes interleukin 11 (IL11), proteoglycan4 (PRG4), NADPH oxidase 4 (NOX4), and decreased the expression of adrenomedullin (ADM), and pentraxin 3 (PTX3) in gingival fibroblasts. Incubation of bone chips with 0.2% chlorhexidine, followed by vigorously washing resulted in a bone-conditioned medium with even higher expression of IL11, PRG4, and NOX4. These findings were also found with a decrease in cell viability and an activation of apoptosis signaling. Chlorhexidine alone, at low concentrations, increased IL11, PRG4 and NOX4 expression, independent of the TGF-β receptor I kinase activity. In contrast, 0.25% sodium hypochlorite almost entirely abolished the activity of bone-conditioned medium, while the other two antiseptic solutions, 1% hydrogen peroxide and 0.5% povidone-iodine, had relatively no impact, respectively. CONCLUSION: These in vitro findings demonstrate that incubation of bone chips with chlorhexidine differentially affects the activity of the respective bone-conditioned medium compared to the other antiseptic solutions. The data further suggest that the main effects are caused by chlorhexidine remaining in the bone-conditioned medium after repeated washing of the bone chips. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved. KEYWORDS: Autografts; TGF-β; antiseptic solution; bone; bone conditioned medium; bone supernatant; chlorhexidine; hydrogen peroxide; povidone-iodine; sodium hypochlorite

Elegia De Eclipsi, Qva in Vmbram Terrae Lvna Incvrrit Ivxta Cor Leonis : D. Vlt. M. Ianvar. H. IIX M. LIIX P. M. Anni Vertentis

Scripta ad Studiosos in Academia Erphordiana, Aut. Barth. Hubnero Philos. & Med. Doctore

Mus Hs 678 - Var: Cor, et Fagott ...

par C. Oestreich

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El presente trabajo pretende ilustrar brevemente las actitudes de tres grupos de hablantes/ alumnos de inglés como lengua extranjera (LE) de entre 20 y 30 años respecto de un conjunto de transferencias léxicas (TL) del inglés en lengua escrita y en el contexto particular del lenguaje publicitario para la comercialización de productos alimenticios de consumo ordinario

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El presente trabajo pretende ilustrar brevemente las actitudes de tres grupos de hablantes/ alumnos de inglés como lengua extranjera (LE) de entre 20 y 30 años respecto de un conjunto de transferencias léxicas (TL) del inglés en lengua escrita y en el contexto particular del lenguaje publicitario para la comercialización de productos alimenticios de consumo ordinario

Light, packs, chips, crackers

El presente trabajo pretende ilustrar brevemente las actitudes de tres grupos de hablantes/ alumnos de inglés como lengua extranjera (LE) de entre 20 y 30 años respecto de un conjunto de transferencias léxicas (TL) del inglés en lengua escrita y en el contexto particular del lenguaje publicitario para la comercialización de productos alimenticios de consumo ordinario

Chronology and petrology of sediment cor DAPC2

Evidence from paleoclimatic archives suggests that Earth's climate experienced rapid temperature changes associated with pronounced interhemispheric asymmetry during the last glacial period. Explanations for these climate excursions have converged on nonlinear interactions between ice sheets and the ocean's thermohaline circulation, but the driving mechanism remains to be identified. Here we use multidecadal marine records of faunal, oxygen isotope, and sediment proxies from the northeast Atlantic proximal to the western margins of the last glacial British Ice Sheet (BIS) to document the coupling between ice sheet dynamics, ocean circulation, and insolation changes. The core data reveal successions of short-lived (80-100 years), high-amplitude ice-rafted debris (IRD) events that were initiated up to 2000 years before the deposition of detrital carbonate during Heinrich events (HE) 1 and 2. Progressive disintegration of the BIS 19-16 kyr before present (B.P.) occurred in response to abrupt ocean-climate warmings that impinged on the northeast Atlantic during the early deglaciation. Peak IRD deposition recurs at 180-220 year intervals plausibly involving repeated breakup of glacial tidewater margins and fringing marine ice shelves. The early deglaciation culminated in a major meltwater pulse at ~16.3 kyr B.P. followed by another discharge associated with HE1 some 300 years after. We conclude that temperature changes related to external forcing and marine heat transport caused a rapid response of the BIS and possibly other margins of the Eurasian Ice Sheet. Massive but short-lived meltwater surges influenced the Atlantic meridional overturning circulation thereby contributing to North Atlantic climate variability and bipolar climatic asymmetry.