The Menkes/Wilson disease gene homologue in yeast provides copper to a ceruloplasmin-like oxidase required for iron uptake.

The CCC2 gene of the yeast Saccharomyces cerevisiae is homologous to the human genes defective in Wilson disease and Menkes disease. A biochemical hallmark of these diseases is a deficiency of copper in ceruloplasmin and other copper proteins found in extracytosolic compartments. Here we demonstrate that disruption of the yeast CCC2 gene results in defects in respiration and iron uptake. These defects could be reversed by supplementing cells with copper, suggesting that CCC2 mutant cells were copper deficient. However, cytosolic copper levels and copper uptake were normal. Instead, CCC2 mutant cells lacked a copper-dependent oxidase activity associated with the extracytosolic domain of the FET3-encoded protein, a ceruloplasmin homologue previously shown to be necessary for high-affinity iron uptake in yeast. Copper restored oxidase activity both in vitro and in vivo, paralleling the ability of copper to restore respiration and iron uptake. These results suggest that the CCC2-encoded protein is required for the export of copper from the cytosol into an extracytosolic compartment, supporting the proposal that intracellular copper transport is impaired in Wilson disease and Menkes disease.

Caníbales y reyes. Marvin Harris

Estudio monográfico del libro de Marvin Harris "Caníbales y reyes". Presentación en PPT.

Letter from William Croswell to Thaddeus Harris, 1820 May 22

Handwritten copy of a one-page letter requesting advice about the Harvard Library Catalogue.

Letter from William Croswell to Thaddeus Harris, 1821 May 16

Draft of a letter regarding the Harvard Library Catalogue.

Insecta Saundersiana : or, Charcters of undescribed insects in the collection of William Wilson Saunders.

v.4 (1858) [Homoptera]

[Admittatur of Thaddeus W. Harris], 1811 September 24

Published copy of the 1814 College Laws with the admittatur of undergraduate Thaddeus W. Harris signed by President John Kirkland on September 24, 1811.

An oration, Thaddeus M Harris A.M. now minister of Dorchester, 1790

This small paper notebook contains a twenty-one-page handwritten oration on learned societies delivered by Phi Beta Kappa member Thaddeus Mason Harris on September 1, 1790. The oration is followed by five pages of "notes and illustrations" on the text. Title transcribed from title page. Item bound in blue-and-white floral wallpaper covers.

Geochronology, stratigraphy, and typology / John Andrew Wilson --.

v.33:no.11(1975)

Letter from William Harris to Samuel Shapleigh, 3 January 1791

Includes response and receipt for payment of £10 from butler Samuel Shapleigh.

Mathematical notebook attributed to Thaddeus Mason Harris, ca. 1787

Hardcover notebook containing handwritten transcriptions of rules, cases, and examples from 18th century mathematical texts. The author and purpose of the volume is unclear, though it has been connected with Thaddeus Mason Harris (Harvard AB 1787). Most of the entries include questions and related answers, suggesting the notebook was used as a manuscript textbook and workbook. The extracts appear to be copied from John Dean's " Practical arithmetic" (published in 1756 and 1761), Daniel Fenning's "The young algebraist's companion" (published in multiple editions beginning in 1750), and Martin Clare's "Youth's introduction to trade and business" (extracts first included in 1748 edition).