Occurrence of enteroparasites in day care centers in Botucatu (São Paulo State, Brazil) with emphasis on Cryptosporidium sp., Giardia duodenalis and Enterobius vermicularis

Verificou-se a prevalência dos enteroparasitas em 279 crianças (0 a 6 anos) de quatro creches municipais de Botucatu/SP. Foram coletadas três amostras de fezes de cada criança e processadas pelos métodos Hoffman, Faust e Ritchie e posterior coloração do esfregaço fecal pelos métodos de Auramina-O e Ziehl-Neelsen modificado para diagnóstico de Cryptosporidium sp. e método da fita gomada para diagnóstico de Enterobius vermicularis. Das crianças analisadas apresentaram-se parasitadas 53.40%, sendo que o parasita mais freqüente foi Giardia duodenalis (26.88%). Verificou-se associação significativa entre enteroparasitose, renda familiar, escolaridade materna e idade; quanto maior a renda e o grau escolar, menor a freqüência de enteroparasitas. Observou-se que G. duodenalis é mais prevalente em crianças de 0 a 4 anos e E. vermicularis em crianças entre três e quatro anos de idade. A elevada prevalência de enteroparasitas em creches sugere estrutura complexa em sua epidemiologia, onde fatores além do saneamento devem ser considerados.

Molecular detection of Leishmania sp. in cats (Felis catus) from Andradina Municipality, São Paulo State, Brazil

The aim of this work was to molecularly detect Leishmania species in 52 cats from Andradina Municipality, São Paulo State, Brazil. The direct parasitological test was performed by using imprints of poplited lymph node, bone marrow and spleen to verify amastigote forms of Leishmania spp. The samples that were positive parasitological tests were subjected to molecular analysis (PCR) and sequencing. Infection was detected for 5.76% (3/52) of the examined cats and two had presence of amastigote forms of Leishmania spp. in lymph nodes. Polymerase chain reaction (PCR) of kinetoplast minicircle DNA, indicated positive amplification for samples of spleen and lymph nodes and the sequencing resulted in 97% similarity with Leishmania (L) chagasi. This study proved the occurrence of infection with Leishmania (L) chagasi in felines from Andradina municipality, São Paulo State. (C) 2010 Elsevier B.V. All rights reserved.

First report of Strongyloides sp. (Nematoda, Strongyloididae) in Leopardus tigrinus (Carnivora: Felidae) in the municipality of Botucatu, State of São Paulo, Brazil

O presente estudo reporta o primeiro caso de infecção por Strongyloides sp. em Leopardus tigrinus no município de Botucatu, Estado de São Paulo, Brasil. Fezes do exemplar parasitado de L. tigrinus foram cultivadas em fezes eqüinas esterilizadas e foi realizada infecção experimental em gato (Felis catus domesticus) com três mil larvas L3 infectantes por via subcutânea, para a identificação da espécie de Strongyloides envolvida no parasitismo. As fêmeas partenogenéticas obtidas do animal experimental foram analisadas porém a comparação dos dados biométricos encontrados com os dados da literatura não permitiu a identificação da espécie. Este é o primeiro relato sobre a ocorrência de Strongyloides sp. em L. tigrinus

Infecção natural de Equus caballus por Leishmania sp - São Paulo, Brasil (Breve comunicação científica)

E assinalado, pela primeira vez, o encontro de um cavalo (Equus caballus) infeclado por Leishmania sp, no Estado de São Paulo.

Population biology of Trichomycterus sp. (Siluriformes, Trichomycteridae) in Passa Cinco stream, Corumbataí River sub-basin, São Paulo State, southeastern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mites (Acari) associated with bamboo (Bambusa sp., Poaceae) in a woody area from northwestern São Paulo State, Brazil

This paper reports twelve acarine species belonging to eleven genera in five families, associated with Bambusa sp. (Poaceae), in a woody area from São Paulo State, Brazil. Four species of Tetranychidae (Monoceronychus bambusicola, sp.n.. Oligonychus santoantoniensis, sp.n.. Schizotetranychus longirostris, sp.n. and S. paraelymus, sp.n.) are regarded as new. The genus Cheletomimus Oudemans, 1904 (Cheyletidae) is recorded for the first time to South America.

Neotropical monogenoidea. 25. Anacanthorus penilabiatus n. sp. (Dactylogyridae, Anacanthorinae) from Piaractus mesopotamicus (Osteichthyes, Serrasalmidae), cultivated in the State of São Paulo, Brazil

Anacanthorus penilabiatus n. sp. is described from the serrasalmid fish, Piaractus mesopotamicus (Holmberg, 1887), cultivated in the Centro de Aqüicultura, Universidade Estadual Paulista. The new species is characterized by having a relatively straight copulatory organ with a long lip on the distal margin and a median longitudinal flap, and a copulatory ligament. The large size of the infrapopulations of this species of parasite indicates that it should be considered a potential agent causing losses in aquaculture of the fish host.

Fatores associados à prática de atividade física global e de lazer em idosos: Inquérito de Saúde no Estado de São Paulo (ISA-SP), Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fatores associados ao tabagismo em idosos: Inquérito de Saúde no Estado de São Paulo (ISA-SP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparação das estimativas de prevalência de indicadores de saúde no Município de Campinas, São Paulo, Brasil, nos anos de 2001/2002 (ISA-SP) e 2008/2009 (ISA-Camp)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Neotropical Monogenoidea .25. Anacanthorus penilabiatus n sp (Dadylogyridae, Anacanthorinae) from Piaractus mesopotamicus (Osteichthyes, Serrasalmidae), cultivated in the State of São Paulo, Brazil

Anacanthorus penilabiatus n. sp. is described from the serrasalmid fish, Piaractus mesopotamicus (Holmberg, 1887), cultivated in the Centro de Aquicultura, Universidade Estadual Paulista. The new species is characterized by having a relatively straight copulatory organ with a long ''lip'' on the distal margin and a median longitudinal flap, and a copulatory ligament. The large size of the infrapopulations of this species of parasite indicates that it should be considered a potential agent causing losses in aquaculture of the fish host.

Prevalência de Cryptosporidium sp. em cães de instituições da cidade de São Paulo

Objective Dogs play an important role as infection source of human cryptosporidiosis. The objective of the study was to determine the prevalence of Cryptosporidium sp. in dogs as well as to compare two techniques of fecal analysis.Methods Four-hundred and fifty canine fecal samples from the city of São Paulo were analyzed between 2003 and 2004. Fecal samples were randomly selected from dogs housed in a university veterinary hospital (group 1, n=200) and private kennels (group 2, n=250). The detection of Cryptosporidium was performed using modified Ziehl-Neelsen staining and Polymerase Chain Reaction (PCR) technique. Statistical analysis was performed using the two-tailed test of significance at 5% confidence interval (z critical=+/- 1.645).Results Only Cryptosporidium parvum oocysts were found the prevalences found by light microscopy examination and PCR techniques were 8.8% and 9.5%, respectively. Young animals showed a lower frequency (5.5%) compared to adults (10.1%). There was no statistically significant difference in Cryptosporidium prevalence between males and females.Conclusions the prevalence of C. parvum in the canine population studied was similar to that one found in the literature and affects equally males and females. The use of PCR allowed the detection of more positive cases than light microscopy.

FIRST RECORD of AN EPIBIONT PROTOZOAN EPISTYLIS SP (CILIOPHORA, PERITRICHIA) ATTACHED TO AMPLEXIBRANCHIUS BRYCONIS THATCHER & PAREDES, 1985 (COPEPODA, ERGASILIDAE) FROM PEIXE'S RIVER, STATE of São Paulo, BRAZIL

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Henneguya nagelii n. sp. (Myxozoa: Myxobolidae) in Cyphocharax nagelii (Steindachner, 1881) (Teleostei: Characiformes: Curimatidae) from the Peixe's River, São Paulo State, Brazil

A new species of Myxosporea, Henneguya nagelii n. sp., is described parasitizing the gills of Cyphocharax nagelii collected from Peixe's River, São Paulo State, Brazil. Among the fish examined, 16.7 % had gills parasitized by myxosporeans. The plasmodia were white, round, or oval and measured 150-250 μm. The mature spores were fusiform and had smooth wall. The spores measurements were the following: total length, 34.5 ± 4.2 (26.4-39.9) μm; body length, 12.0 ± 0.5 (11.2-11.9) μm; body width, 4.9 ± 0.3 (4.4-5.5) μm; and caudal process length, 22.4 ± 4.0 (14.7-27.3) μm. The polar capsules were elongated and of unequal size, with lengths of 4.9 ± 0.4 (4.0-5.9) μm and 5.2 ± 0.4 (4.6-6.0) μm for the longest and shortest axes, respectively. Capsule width was 1.8 ± 0.2 (1.5-2.2) μm. Each capsule contained a polar filament with six to eight turns. There was no mucous envelope or iodinophilous vacuole. Morphometric differences between this parasite and other species of the genus Henneguya indicated that the parasite observed in C. nagelii is a new species. This is the first species of Myxosporea described in Peixe's River. © 2013 Springer-Verlag Berlin Heidelberg.

Ocorrência de Leishmania sp. em gatos do município de Araçatuba - São Paulo - Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)