On the ecology and explorations of the fisheries of an East African rift valley lake, Part 1: On the bionomics and population structure of Tilapia nilotica Linnaeus 1757 in Lake Baringo, Kenya

An ecological survey of the fisheries of Lake Baringo, Kenya was carried out between August, 1972 and August, 1973. The bionomics and population structure of T. nilotica is described. Sampling was done with multifilament gillnets of graded mesh sizes from 51 mm to 178 mm in approximately 12.5 mm increments. The Lake was divided into three sampling and ecologically different zones - the south, central and north zones. The size range of T. nilotica of both sexes caught was between 5 and 27 cm (mode 16 cm) with a mean length of 16.07 cm. For all the collections, males dominated (55.3%) and a higher proportion of males were caught in January, August and November. The smallest mature male and female was 9 and 10 cm respectively. Males grow faster and mature at larger sizes than females. 50% of all males and females mature at 17.4 and 16:4 cm respectively. The periods of intense spawning were between August and October and January to April. The Tilapia were feeding best in central and north zones and the feeding intensity was reduced in January. Two endoparasites Contracaecum sp. and Clinostomum sp. were isolated from the Tilapia. The "condition" of the fish was better in the north than in the other two zones.

Hybridization between Tilapia zillii (Gervais) and Tilapia andersonii (Casteinau) at the Freshwater Fisheries Institute, at Nyegezi, Tanzania

As a part of an overall project on fishculture development techniques in Tanzania, hybridization between Tilapia zillii and Tilapia andersonii was carried out at the Freshwater Fisheries Institute, Nyegezi, Tanzania. T. andersonii, a plankton feeder, is not indigenous to Tanzania but was introduced in 1968 from Zambia for certain specific purpose. T. zillii, a macrovegetation feeder, is present locally and is common. In the present studies T. zillii (245.0 mm/260.0 g) female was hybridized with T. andersonii (288.0 mm/350.0 g) male. Under cement cistern conditions it was only after about four months of acclimatization that hybridization between the two occurred. About 1,637 interspecific hybrid fry were produced in a single brood. Eggs were adhesive and parental care shown by the female, the male being driven away. Growth under cistern conditions was slow, attaining a size of 134.8 mm/44.3 g in 10 months. But this growth rate need not be taken as ideal. In body shape, colouration and other morphometric characters the hybrids had inherited from both parents. The number of gill rakers among the hybrids was eighteen which was intermediate between T. zillii (12) and T. andersonii (27). Among one hundred and seventy two specimens (106.0 mm - 168.0 mm) cut and examined the sex ration was hundred per cent males and all of them were between II and IV stages of maturity. This is the first report of fish hybridization from Tanzania and possibly the first report on hybridization between T. zillii and T. andersonii. The full significanoe of the findings and its role in African fishculture is discussed.

Evaluation of external anchor tags for estimation of growth and survival in tilapia (Oreochromis shiranus chilwae Trewavas)

The effects of tagging with Roy FD-68B T-bar anchor tags on estimates of growth in Tilapia (Oreochromis shiranus chilwae) were investigated in a pond and in a field experiment. In the pond experiment, mean length increments of tagged and marked fish were compared. In the field experiment growth of tagged and "untouched" individual wild fish were compared by measuring scale circuli spacing (Circ.), which is correlated to instantaneous growth rate. Length increments of tagged and untagged/marked fish were not significantly different in either experiment. In the pond experiment, the total mortality rate in the small tagged fish was significantly higher than in the marked fish. The recoveries of tagged fish in the pond experiment increased with fish size. Recoveries exceeded 80% at lengths over 13 cm TL. The ratios of tagged to marked recoveries were 1.02 and 0.74 for large and small fish respectively. The study shows that tagging of Tilapia with Roy anchor tags does not in general alter the growth rates of the fish.

A note on recent advances in the genetic characterization of tilapia stocks in Lake Victoria region

Oreochromis esculenta, the original "ngege" is virtually extinct in Lake Victoria, and is limited to satellite lakes and reservoirs in the greater Lake Victoria region. Oreochromis variabilis can still be found in Lake Victoria and some satellite lakes in the Kyoga System, but in small numbers and only at a few localities (WANDERA and KAUFMAN, unpub. data). Little is known about the influence that species translocations have had on the genetic structure of these crucial fishery species, and even the source of the parent stocks for the introductions remain obscure. Genetic variability was examined within and among allopatric populations of three species in the tilapiine genus Oreochromis: O. esculentus (endemic to Lakes Victoria and Kyoga), and two exotic species introduced to Lake Victoria in the late 1950's to supplement the failing fisheries for native tilapiines, O. niloticus and O. leucostictus.

尼罗罗非鱼Tilapia nilotica卵巢发育过程中液晶态类脂滴组织化学初步研究

<正> 我们研究了尼罗罗非鱼卵巢中的液晶态。用偏光显微镜观察到在第3和第4时相卵母细胞的胞质、滤泡细胞和部份间质区存在着双折射和多种形式的“马尔它十字”。电子显微镜观察到这些“马尔它十字”小滴是多层的膜结构。根据这些小滴的性质和结构,将其归属于溶致性液晶(lyotropic liquid crystal)的片层相(lamellar phase),即纯净相(neat phase)(孙建民1981)。

Effects of dietary cyanobacteria of two different sources on growth and recovery of hybrid tilapia (Oreochromis niloticus x O. aureus)

A 115 days feeding trial was conducted to evaluate the effect of dietary cyanobacteria on growth, microcystins (MCs) accumulation in hybrid tilapia (Oreochromis niloticus x O. aureus) and the recovery when the fish were free of cyanobacteria. Three experimental diets were formulated: the control (cyanobacteria free diet); one test diet with cyanobacteria from Lake Taihu (AMt 80.0 mu g MCs g(-1) diet) and one with cyanobacteria from Lake Dianchi (AMd, 410.0 rho g MCs g(-1) diet). Each diet was fed to fish for 60 days and then all fish were free of cyanobacteria for another 55 days. A significant increase in feeding rate (FR) was observed in fish fed AMd diet after a first 30-day exposure (1(st) EP), and in fish fed both AMt diet and AMd diet after a second 30-day exposure (2(nd) EP). Specific growth rates (SGR) of fish fed AMt diet and AMd diet were both obviously affected after the first 30-day exposure, but SGR was only significantly affected in fish fed AMt diet after the second 30-day exposure. After a 55-day recovery, there were no significant differences among diets in the indices mentioned above. Much higher concentrations of MCs were accumulated in tissues of all fish exposed to cyanobacteria. After the 55-day recovery, MC concentrations in fish tissues were significantly lower than those on day 60. (C) 2009 Elsevier Ltd. All rights reserved.

Detection and quantitative analysis of polychlorinated biphenyls in tilapia from Hawaiian waters

The purpose of this study was to investigate polychlorinated biphenyls (PCBs) contamination in tilapia (Oreochromis mossambicus) collected from the Manna stream and Ala Wai Canal of O'ahu, an island of the geographically isolated Hawaiian archipelago. Our results show that the average concentrations of PCBs varied from 51.90 to 89.42 ng g(-1) lipid weight for the sampling sites. Relative toxic potencies (RTPs) and toxic equivalencies (TEQs) were determined to be 20.38-40.60 ng TCDD g(-1) lipid weight and 2.89-4.17 ng TEQ g(-1) lipid weight by 7-ethoxy-resorufin-O-deethylase (EROD) activity analysis and calculation of PCB concentrations based on toxic equivalency factors (TEFs), respectively. Penta-chlorinated congeners were found to be predominant, which revealed that Aroclor 1254 was a possible major source of PCBs in our fish samples. PCB 118, an indicator PCBs, constituted more than 55% and 30% of the total PCBs and TEQs, respectively. In addition, PCB 118 was found to have a linear correlation to the total PCBs (R = 0.975) and TEQs (R = 0.782). Detection of concentrated PCBs in Hawaiian waters suggests a potentially adverse impact of this pollutant on human health, as well as ecological systems, and suggests the necessity of environmental monitoring and hazard assessment of PCBs within the Hawaiian Islands. (c) 2008 Published by Elsevier Ltd.

Detection of polybrominated diphenyl ethers in tilapia (Oreochromis mossambicus) from O'ahu, Hawai'i

Polybrominated diphenyl ethers (PBDEs) have been detected for the first time at a range from 231.58 to 685.61 ng g(-1) lipid weight in the muscles of tilapia ( Oreochromis mossambicus) collected from O'ahu, an island of the geographically isolated Hawaiian archipelago.

Evaluation of estrogenic activities and mechanism of action of perfluorinated chemicals determined by vitellogenin induction in primary cultured tilapia hepatocytes

Perfluorochemicals (PFCs) are emerging persistent organic pollutants (POPs) and are widely present in the environment, wildlife and humans. Recently, reports have suggested that PFCs may have endocrine-disrupting activities. In the present study, we have developed a non-competitive enzyme-linked immunosorbent assay (ELISA) method to investigate estrogenic activities of selected PFCs using vitellogenin (VTG) induction in primary cultured hepatocytes of freshwater male tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to various concentrations of perfluorooctanyl sulfonate (PFOS), pentadecafluorooctanoic acid (PFOA), 1H, 1H, 2H, 2H-nonafluoro-1-hexanol (4:2 FTOH), 1H, 1H, 2H, 2H-perfluorooctanol (6:2 FTOH) and 1H, 1H, 2H, 2H-perfluoro-1-decanol (8:2 FTOH) for 48h, while 17 beta-estradiol (E2) and 4-nonylphenol (4-NP) were used as positive controls. A dose-dependent induction of VTG was observed in E2-, 4-NP-, PFOS-, PFOA- and 6:2 FrOH-treated cells, whereas VTG levels remained unchanged in the 4:2 FTOH and 8:2 FTOH exposure groups at the concentrations tested. The estimated 48-h EC50 values for E2,4-NP, PFOS, PFOA and 6:2 FTOH were 4.7 x 10(-7), 7.1 x 10(-6), 1.5 x 10(-5), 2.9 x 10(-5) and 2.8 x 10(-5) M, respectively. In the time-course study, significant VTG induction took place at 24 h (E2), 6 It (4-NP), 48 It (PFOS), 48 It (PFOA), 72 It (4:2 FTOH), 12 h (6:2 FTOH), 72 h (8:2 FTOH), and increased further after 96 It of exposure. Co-exposure to binary mixtures of individual PFCs and E2 for 48 It significantly inhibited E2-induced hepatocellular VTG production in a dose-dependent manner except for 4:2 FTOH. The estimated 48-h IC50 (concentration of a compound that elicits 50% inhibition of maximally E2-induced VTG) values for PFOS, PFOA, 6:2 FTOH and 8:2 FTOH were 3.1 x 10(-7), 5.1 X 10(-7), 1.1 X 10(-6) and 7.5 x 10(-7) M, respectively. In order to further investigate the estrogenic mechanism of PFCs, the hepatocytes were co-exposed to binary mixtures of individual chemicals (E2,4-NP, PFOS, PFOA and 6:2 FTOH) and the known estrogen receptor inhibitor tamoxifen for 48 h; tamoxifen significantly inhibited the ability of these chemicals to stimulate vitellogenesis. The overall results demonstrated that PFOS, PFOA and FTOHs have estrogenic activities and that exposure to a combination of E2 and PFCs produced anti-estrogenic effects. The results of the estrogen receptor inhibition assay further suggested that the estrogenic effect of PFCs may be mediated by the estrogen receptor pathway in primary cultured tilapia hepatocytes. (c) 2007 Elsevier B.V. All rights reserved.

Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)

Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. (c) 2007 Elsevier B.V. All rights reserved.

Effect of dietary cyanobacteria on growth and accumulation of microcystins in Nile tilapia (Oreochromis niloticus)

A 12-week growth trial was conducted in a flow-through system to investigate the chronic toxic effect of dietary intake of cyanobacteria on growth, feed utilization and microcystins accumulation in Nile tilapia (Oreochromis niloticus L.) (initial body weight: 5.6 g). Six isonitrogenous and isocaloric diets were formulated to include different contents of cyanobacteria with the dietary microcystins increasing from 0 to 5460.06 ng/g diet. The results showed that dietary intake of cyanobacteria could increase the growth of tilapia while there are no impacts on feed conversion efficiency or mortality. Feeding rate was higher for the diets containing highest cyanobacteria. Microcystins were mostly accumulated in fish liver. The relationship between microcystins contents in muscle, liver, spleen and dietary intake could be described by quadratic equations. Microcystins content in the muscle of Nile tilapia in present study exceeded the upper limit of the tolerable daily intake (TDI) of microcystins suggested by the WHO (0.04 mu g/kg body weight/d). It is suggested that Nile tilapia fed on toxic cyanobacteria is not suitable for human food. (c) 2006 Elsevier B.V. All rights reserved.

Partial compensatory growth in hybrid tilapia Oreochromis mossambicus x O-niloticus following food deprivation

The capacity of hybrid tilapia Oreochromis mossambicus x O. niloticus [23.2 +/- 0.2 g (mean +/- SE)] to show compensatory growth was assessed in an 8-week experiment. Fish were deprived of feed for 1, 2 and 4 weeks, and then fed to satiation for 4 weeks; fish fed to satiation during the experiment served as control. Water temperature gradually declined from 28.1 to 25.5 degrees C throughout the experiment. Specific growth rate (SGR) decreased with progressive food deprivation. At the end of deprivation, body weight was lower in the deprived fish than in the control. Fish deprived for 4 weeks exhibited lower contents of lipids and energy in whole body, and higher moisture content and ratio of protein to energy (P/E) than those of the control; they also consumed feed faster than the control when normal feeding was resumed. All deprived fish showed higher food intake (FI) than that of the control during re-alimentation; however, enhanced SGR was only observed in the fish deprived for 4 weeks. There were no significant differences in digestibility of protein and energy, food efficiency (FE) or energy retention efficiency between the control and deprived fish. At the end of re-alimentation, deprived fish failed to catch up in body weight with the control, while content of moisture, lipids and energy, and P/E in whole body of the deprived fish did not significantly differ from that of the control. The results of the experiment revealed that the hybrid tilapia reared in freshwater showed partial capacity for compensatory growth following food deprivation of 4 weeks, and that growth compensation was due mainly to increased FI, rather than to improved FE.

Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants

A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved.

Effects of several organic acids on the feeding behavior of Tilapia nilotica

Automatic recording of the frequency of feeding 'bites' was used to evaluate the effects of several organic acids (citric, metacectonic, lactic, acetic, and oxalic) on the stimulatory feeding behavior of Tilapia nilotica . Some of these acids are added to food stocks to retard spoilage. The results showed that citric acid at a concentration of 10(-2) to 10(-6) m, metacetonic acid at 10(-4) to 10(-6) m, and lactic acid at 10(-2) to 10(-5) m stimulated feeding. Fish tended to avoid metacetonic acid at 10(-3) m and acetic acid at 10(-3) m. Acetic acid at 10(-5) m and oxalic acid at 10(-6) m had no significant effects on fish feeding.