Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity

The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly against multidrug resistant strains of Acinetobacter baumannii. We have cloned, heterologously expressed and characterized a novel endolysin (ABgp46) from Acinetobacter phage vb_AbaP_CEB1 and tested its antibacterial activity against several multidrug-resistant A. baumannii strains. LC-MS revealed that ABgp46 is an N-acetylmuramidase, that is also active over a broad pH range (4.0-10.0) and temperatures up to 50°C. Interestingly, ABgp46 has intrinsic and specific anti-A. baumannii activity, reducing multidrug resistant strains by up to 2 logs within 2 hours. By combining ABgp46 with several organic acids that act as outer membrane permeabilizing agents, it is possible to increase and broaden antibacterial activity to include other Gram-negative bacterial pathogens. In the presence of citric and malic acid, ABgp46 reduces A. baumannii below the detection limit (> 5 log) and more than 4 logs P. aeruginosa and Salmonella Typhimurium strains. Overall, this globular endolysin exhibits a broad and high activity against Gram-negative pathogens, that can be enhanced in presence of citric and malic acid, and be used in human and veterinary medicine.

Structural and functional characteristics of rat hearts with and without myocardial infarct. Initial experience with doppler echocardiography

OBJECTIVE: To assess by Doppler echocardiography the structural and functional alterations of rat heart with surgical induced extensive myocardial infarction. METHODS: Five weeks after surgical ligature of the left coronary artery, 38 Wistar-EPM rats of both sexes, 10 of them with extensive infarction, undergone anatomical and functional evaluation by Doppler echocardiography and then euthanized for anatomopathological analysis. RESULTS: Echocardiography was 100% sensible and specific to anatomopathological confirmed extensive miocardial infarction. Extensive infarction lead to dilatation of left ventricle (diastolic diameter: 0.89cm vs.0.64cm; systolic: 0.72cm vs. 0.33cm) and left atrium (0.55cm vs. 0.33cm); thinning of left ventricular anterior wall (systolic: 0.14cm vs. 0.23cm, diastolic: 0.11cm vs. 0.14cm); increased mitral E/ A wave relation (6.45 vs. 1.95). Signals of increased end diastolic ventricle pressure, B point in mitral valve tracing in 62.5% and signs of pulmonary hypertension straightening of pulmonary valve (90%) and notching of pulmonary systolic flow (60%) were observed in animals with extensive infarction. CONCLUSION: Doppler echocardiography has a high sensitivity and specificity for detection of chronic extensive infarction. Extensive infarction caused dilatation of left cardiac chambers and showed in Doppler signals of increased end diastolic left ventricular pressure and pulmonary artery pressure.

The London journal of botany : containing figures and descriptions of such plants as recommend themselves by their novelty, rarity, history, or uses : together with botanical notices and information and and occasional portraits and memoirs of eminent botanists.

v.2 (1843)

The London journal of botany : containing figures and descriptions of such plants as recommend themselves by their novelty, rarity, history, or uses : together with botanical notices and information and and occasional portraits and memoirs of eminent botanists.

v.3 (1844)

The London journal of botany : containing figures and descriptions of such plants as recommend themselves by their novelty, rarity, history, or uses : together with botanical notices and information and and occasional portraits and memoirs of eminent botanists.

v.4 (1845)

The London journal of botany : containing figures and descriptions of such plants as recommend themselves by their novelty, rarity, history, or uses : together with botanical notices and information and and occasional portraits and memoirs of eminent botanists.

v.5 (1846)

The London journal of botany : containing figures and descriptions of such plants as recommend themselves by their novelty, rarity, history, or uses : together with botanical notices and information and and occasional portraits and memoirs of eminent botanists.

v.6 (1847)

The London journal of botany : containing figures and descriptions of such plants as recommend themselves by their novelty, rarity, history, or uses : together with botanical notices and information and and occasional portraits and memoirs of eminent botanists.

v.7 (1848)

Prodromus Florae Peninsulae Indiae Orientalis :containing abridged descriptions of the plants found in the peninsula of British India, arranged according to the natural system : Vol. 1 /by Robert Wight and G.A. Walker-Arnott.

1834

Plants of the coast of Coromandel :selected from drawings and descriptions presented to the hon. court of directors of the East India Company /by William Roxburgh.

v.1

Plants of the coast of Coromandel :selected from drawings and descriptions presented to the hon. court of directors of the East India Company /by William Roxburgh.

v.2

Plants of the coast of Coromandel :selected from drawings and descriptions presented to the hon. court of directors of the East India Company /by William Roxburgh.

v.3