Obtenção dos parâmetros e estudo cinético da hidrólise ácida dos resíduos florestais de eucalipto

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação do processo de produção de cefamicina C por Streptomyces clavuligerus

Pós-graduação em Biotecnologia - IQ

Otimização do cultivo de Lasiodiplodia theobromae para obtenção de EPS e caracterização física e química dos polissacarídeos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de suportes para a imobilização de Zymomonas mobilis CCT 4494 visando à produção de etanol e levana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Characterization of the co-purified invertase and beta-glucosidase of a multifunctional extract from Aspergillus terreus

The filamentous fungus Aspergillus terreus secretes both invertase and beta-glucosidase when grown under submerged fermentation containing rye flour as the carbon source. The aim of this study was to characterize the co-purified fraction, especially the invertase activity. An invertase and a beta-glucosidase were co-purified by two chromatographic steps, and the isolated enzymatic fraction was 139-fold enriched in invertase activity. SDS-PAGE analysis of the co-purified enzymes suggests that the protein fraction with invertase activity was heterodimeric, with subunits of 47 and 27 kDa. Maximal invertase activity, which was determined by response surface methodology, occurred in pH and temperature ranges of 4.0-6.0 and 55-65 A degrees C, respectively. The invertase in co-purified enzymes was stable for 1 h at pH 3.0-10.0 and maintained full activity for up to 1 h at 55 A degrees C when diluted in water. Invertase activity was stimulated by 1 mM concentrations of Mn2+ (161 %), Co2+ (68 %) and Mg2+ (61 %) and was inhibited by Al3+, Ag+, Fe2+ and Fe3+. In addition to sucrose, the co-purified enzymes hydrolyzed cellobiose, inulin and raffinose, and the apparent affinities for sucrose and cellobiose were quite similar (K-M = 22 mM). However, in the presence of Mn2+, the apparent affinity and V-max for sucrose hydrolysis increased approximately 2- and 2.9-fold, respectively, while for cellobiose, a 2.6-fold increase in V-max was observed, but the apparent affinity decreased 5.5-fold. Thus, it is possible to propose an application of this multifunctional extract containing both invertase and beta-glucosidase to degrade plant biomass, thus increasing the concentration of monosaccharides obtained from sucrose and cellobiose.

Formation of titania nanotube arrays by anodisation: DOE approach

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Enhancing effect of lysine combined with other compounds on cephamycin C production in Streptomyces clavuligerus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization and optimization of levan production by Bacillus subtilis NATTO

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ajuste preciso do nível de energia na dieta de frangos de corte para controle do desempenho e da composição lipídica da carne

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Optimizing xanthophyll concentrations in diets to obtain well-pigmented yolks

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Acurácia e precisão na formulação não linear de ração para frangos de corte: avanço e otimização de resultados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sucrose utilization by Zymomonas mobilis: Levan production optimization using submerged fermentation

Levan is an extracellular polysaccharide (EPS), constituted by linked fructose units β (2,6), obtained by transfructosilation reaction during fermentation of microorganisms in a sucrose rich culture medium. The bacterial levan production is a good alternative of fructose source, besides having certain functional characteristics in the human body, such as a hypocholesterolemic and an anticarcinogenic agent. In the food industry, the levan can be used to fix colors and flavors, as well as to thickening and stabilizing agent in foods. This work aimed to analyze the kinetic parameters for levan production by Zymomonas mobilis CCT 4494, using submerged fermentation. The response surface methodology (RSM), was utilized to predict the optimization of medium for exopolymer production and the independent variables studied were: initial pH, incubation temperature, sucrose, KCl, K2SO4, MgSO4 and CaCl2. It was observed that the bacterium Z. mobilis CCT 4494 well adapted in medium containing high concentrations of sucrose.

Produção de 1,3-propanodiol por Klebsiella pneumoniae e Escherichia coli: otimização de meio e clonagem de genes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chicken fat and inorganic nitrogen source for lipase production by Fusarium sp. (Gibberella fujikuroi complex)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo do comportamento reológico de sucos combinados de frutas vermelhas

The aim of this work was to evaluate the rheological behavior of combined juices of strawberry, blackberry and raspberry in two different temperatures of interest in industrial processes in the food industry, using rheological analyses on non-oscillatory regime and the Response Surface Methodology (RSM). The rheological data were fitted by the Casson model. All samples of combined juices of ref fruits showed a Newtonian behavior and the rheological parameters yield stress (Kc) and plastic viscosity of Casson (Koc) had a decrease in its values with the temperature increase. By the analysis of the ternary diagrams it is evident that the raspberry juice effectively contributed to the higher values of yield stress and plastic viscosity of Casson in both temperatures studied.