Intravital and whole-organ imaging reveals capture of melanoma-derived antigen by lymph node subcapsular macrophages leading to widespread deposition on follicular dendritic cells.

Aberrant antigens expressed by tumor cells, such as in melanoma, are often associated with humoral immune responses, which may in turn influence tumor progression. Despite recent data showing the central role of adaptive immune responses on cancer spread or control, it remains poorly understood where and how tumor-derived antigen (TDA) induces a humoral immune response in tumor-bearing hosts. Based on our observation of TDA accumulation in B cell areas of lymph nodes (LNs) from melanoma patients, we developed a pre-metastatic B16.F10 melanoma model expressing a fluorescent fusion protein, tandem dimer tomato, as a surrogate TDA. Using intravital two-photon microscopy (2PM) and whole-mount 3D LN imaging of tumor-draining LNs in immunocompetent mice, we report an unexpectedly widespread accumulation of TDA on follicular dendritic cells (FDCs), which were dynamically scanned by circulating B cells. Furthermore, 2PM imaging identified macrophages located in the subcapsular sinus of tumor-draining LNs to capture subcellular TDA-containing particles arriving in afferent lymph. As a consequence, depletion of macrophages or genetic ablation of B cells and FDCs resulted in dramatically reduced TDA capture in tumor-draining LNs. In sum, we identified a major pathway for the induction of humoral responses in a melanoma model, which may be exploitable to manipulate anti-TDA antibody production during cancer immunotherapy.

Central-Verein-Zeitung : Blätter für Deutschtum und Judentum ; C-V-Zeitung ; Organ des Central-Vereins Deutscher Staatsbürger Jüdischen Glaubens

Periodizität: wöchentl.

Organ inflammation in porcine Escherichia coli sepsis is markedly attenuated by combined inhibition of C5 and CD14.

Sepsis is an infection-induced systemic inflammatory syndrome, potentially causing organ failure. We previously showed attenuating effects on inflammation, thrombogenicity and haemodynamics by inhibiting the Toll-like receptor co-factor CD14 and complement factor C5 in a porcine Escherichia coli-induced sepsis model. The present study explored the effect on organ inflammation in these pigs. Tissue samples were examined from the combined treatment group (n = 8), the positive (n = 8) and negative (n = 6) control groups after 4h of sepsis. Inflammatory biomarkers were measured using ELISA, multiplex and qPCR analysis. Combined inhibition of C5 and CD14 markedly attenuated IL-1β by 31-66% (P < 0.05) and IL-6 by 54-96% (P < 0.01) in liver, kidney, lung and spleen; IL-8 by 65-100% in kidney, lung, spleen, and heart (P < 0.05) and MCP-1 by 46-69% in liver, kidney, spleen and heart (P < 0.05). Combined inhibition significantly attenuated tissue factor mRNA upregulation in spleen (P < 0.05) and IP-10 mRNA upregulation in four out of five organs. Finally, C5aR mRNA downregulation was prevented in heart and kidney (P < 0.05). Combined inhibition of C5 and CD14 thus markedly attenuated inflammatory responses in all organs examined. The anti-inflammatory effects observed in lung and heart may explain the delayed haemodynamic disturbances observed in septic pigs receiving combined inhibition of C5 and CD14.

Nationalzeitung : Organ für die gesamten Interessen des jüdischen Volkes

Periodizität: zweimal wöchentl.

Die Stimme : Organ der Allgemeinen Zionisten in Österreich

Zionistischer Landesverband in Österreich

Israelitische Rundschau : offizielles Organ d. Zionistischen Vereinigung für Deutschland ; Centralblatt für die jüdischen Vereine

Wöchentl.

Jüdische Zeitung : National-Jüdisches Organ

Periodizität: 14-tägl.; anfangs wöchentl.

Central-Verein-Zeitung : Blätter für Deutschtum u. Judentum ; C-V-Zeitung ; Organ des Central-Vereins Deutscher Staatsbürger Jüdischen Glaubens

1931,Dez. nicht ersch.; teils ohne Zählung; Periodizität: monatl.

Der treue Zionswächter : Organ zur Wahrung der Interessen des orthodoxen Judenthums

Periodizität: unregelmäßig

Jüdische Presse : Organ für die Interessen des orthodoxen Judentums

Periodizität: wöchentl.

Mitteilungen zur jüdischen Volkskunde : Organ d. Gesellschaft für Jüdische Volkskunde in Hamburg u. d. Gesellschaft für Sammlung und Konservierung von Kunst- und Historischen Denkmälern des Judentums in Wien

Ab 1908 entfällt N.F

Organ- und Nährpräparate und ihre rituelle Zulässigkeit [Rezension]

Stein

Das Organ des Fürsten Uchtomsky gegendie Gegner des Zionismus

Gt.