抗顶体反应人精子单克隆抗体的制备和鉴定

精子从男性生殖道释放后必须经过获能和顶体反应，才能与卵子结合。获能和顶体反应后，精子细胞内和精子表面的许多大分子都会发生改变。利用这些变化，可探索新的避孕方法和男性不育诊断及治疗的新途径。本文将正常人精子于体外在BWW－BSA培养基中获能，用钙离子载体A23187诱导人精子进行顶体反应，以三染和金霉素荧光染色两种方法检测这些精子的顶体反应率为50％左右，然后用这些经顶体反应处理的新鲜人精子（AR sperm）作为抗复，腹腔免疫Balb/C小鼠，利用最近发展起来的半固体培养及普通的液体培养方法制备单抗。分别用未经任何处理的人精子（NT sperm）及上述顶体反应的人精子包被酶标板，用ELISA法检测所得杂交瘤细胞分泌的抗体，得到了近百株阳性杂交瘤，其中已克隆并得到腹水的有23株，并对这些单抗进行了以下鉴定：腹水滴度测定；抗体分类；与人白细胞系U937，Raji和Jurkat的交叉反应；在NT和AR人精子、树（左鼠右句）和小鼠精子上的荧光定位；单抗对人精子的凝集（SA）和制动（SI）试验；免疫印迹测定单抗相应抗原的分子量。主要结果如下：1.单抗与NT和AR精子反应的ELISA结果表明，有12个单抗主要与AR精子抗原发生反应（A组抗体），6个单抗主要与NT精子抗原反应（B组抗体），而另外5个则与这两种精子抗原都呈阳性反应（C组抗体）。2.在得到的23个单抗中，绝大多数（21个）为IgM，只有两个分别是IgC_1和IgG_(2a)。3.23个单抗与白细胞系的交叉反应强度不同。A组单抗的交叉反应有的较强，有的较弱，有的居中；B组单抗的交叉反应为弱阳性或阴性；C组单抗则呈现要么很强、要么很弱的交叉反应。4.所得单抗的荧光定位主要在赤道板和中段，未发现定位于顶体及顶体后的单抗，而国内外其他实验室已获得的单抗，主要定位在顶体。某些单抗在两种不同精子（NT sperm, AR sperm）上有不同的荧光定位。这些结果表明，AR精子的免疫原性是十分特殊的，它明显不同于NT精子。5.有9个单抗显示较强的精子凝集作用，另有9个单抗的凝集作用稍弱，未发现有精子制动效应的单抗。6.免疫印迹结果表明，有9个单抗的靶抗原是蛋白质类物质，其分子量为16－146kDa，其余14个单抗的免疫印迹呈阴性反应。有关这些单抗及其抗原的鉴定仍在进行中。其中10个单抗已送世界卫生组织（WHO），参与WHO的抗人精子抗原的单克隆抗体的研究计划。

Extracting the Mechanical Properties of 1D Nanostructures from Nanoindentation

<正>The so-called one dimensional(1D) nanostructures or wirelike nanoentities,such as nanowire(NW),nanotube(NT),and nanobelt(NB) have attracted much interest in scientific community because of their remarkable mechanical,electrical,thermal properties and potential applications in wide variety of devices.The mechanical failure of 1D nanostructures can lead to the malfunction or even failure of entire device and 1D nanostructures may also have size-dependent properties. Therefore,an accurate measurement of their mechanical properties is of

计算机远程控制RIBLL系统真空监测系统

本文介绍了兰州放射性核素次级束流装置 ( RIBL L)中真空的计算机远程读出监视系统的设计和实现。着重说明了 RIBL L上使用的各种不同真空计同计算机通信的端口及利用 Microsoft Visuual C++编写的基于 Windows NT或 Windows95的读出及控制程序流程

Experimental study of the beta-delayed proton decays of Er-145,Er-147

The beta-delayed proton decays of Er-145,Er-147 have been studied experimentally using the Ni-58 + Mo-92 reaction at beam energy of 383 MeV. On the basis of a He-jet apparatus coupled with a tape transport system, the beta-delayed proton radioactivities both from the nu s(1/2) ground state and the nu h(11/2) isomer in Er-145,Er-147 were observed by proton-gamma coincidence measurements. By analyzing the time distributions of the 4(+) -> 2(gamma)(+) transitions in the granddaughter nuclei Dy-144,Dy-146, the half-lives of 1.0 +/- 0.3 s and 1.6 +/- 0.2 s have been deduced for the nu h(11/2) isomers in Er-145,Er-147, respectively.

Dipole bands in nucleus Pm-139

High-spin states in nucleus Pm-139 have been studied using the reaction Cd-116(Al-27, 4n)Pm-139. Two dipole cascades have been found. Spin and parity assignments were based on the Directional Correlation of Oriented Nuclei (DCO) ratios and systematic behavior in neighboring odd-proton nuclei. The level structures of Pm-139 are compared with those of the N = 78 isotone Eu-141 in which two dipole bands have been confirmed as magnetic rotational bands. The close similarity between them suggests that the dipole bands in Pm-139 may be magnetic rotational bands.

A New High-Spin Level Scheme of I-128

High-spin states in the odd-odd nucleus I-128 are investigated via the Sn-124(Li-7,3n)I-128 reaction at 28 and 32 MeV beam energies. A new level scheme of I-128 is established up to high-spin states at I-pi = 16, including 48 levels and 72 gamma transitions. The present level scheme is largely different from the one in a recent publication due to identification of several doublet and triplet gamma transitions and their proper placements in the level scheme. The high-spin level structure exhibits no obvious collective properties and is possibly associated with two and multi-quasiparticle configurations.

Band properties of the transitional nucleus Pt-189

High-spin states in Pt-189 have been studied with the in-beam gamma-spectroscopy method via the Yb-176(O-18, 5n) reaction at beam energies of 88 MeV and 95 MeV. A new level scheme of Pt-189 has been established. Rotational bands based on the upsilon i(13/2)(-1), upsilon f(5/2)(p(3/2)) and upsilon i(13/2)(-2)upsilon f(p(3/2)) configurations, as well as several structures with irregular level spacings, have been observed. Properties of rotational bands have been analyzed in the framework of triaxial particle-rotor model. A gamma similar to -30 degrees triaxial shape and a near prolate shape have been proposed to the upsilon i(13/2)(-1) and uf(5/2)(p(3/2)) bands, respectively. Two Delta I=2 transition sequences with similar energies have been observed, and they have been proposed to be associated with the upsilon i(13/2)(-1)upsilon f(5/2)(p(3/2)) configuration. According to the relevant Nilsson orbitals, the bands built on the upsilon i(13/2)(-1)upsilon f(5/2)(p(3/2)) configuration could be interpreted as a pair of pseudo-spin partner.

HIRFL 束流诊断系统软件的设计

本论文根据兰州重离子加速器（HIRFL）束流诊断系统的现状及改造要求，开发了多任务的束流诊断系统应用软件，它能够通过计算机网络进行测量。该软件的设计采用了面向对象程序设计技术；论文还完成了荧光靶图像获取与处理系统应用软件的开发。这两个应用软件都是由Visual C++编译器来实现，并运行于Windows 95/98操作系统中。论文包括三个部分。第一部分综述了目前国际上加速器束流诊断技术的现状及其发展的新动向，并扼要地介绍了一些重要束流参数的测量方法。第二部分简单介绍了数字图像处理技术的基本内容，比较系统地介绍了荧光靶图像获取及处时系统软件的设计过程，并给出了用该系统测量ECR源束线上束流横向发射度的结果。第三部分首先介绍了Windows操作系统的多任务机制以及计算机网络的通信机制，其中包括TCP/IP协议的有关特性以及如何利用MFC中的CSocket类实现Windows 95/NT系统中网络通信的方法；然后详细介绍了多任务束流诊断系统软件的设计方法及实现过程；最后给出它的现场测试结果以及作者对仿后该软件的升级和维护的设想及展望。

Difference of ZSM-5 zeolites synthesized with various templates

ZSM-5 zeolites with similar SUM ratio were synthesized successfully using various templates (n-butylamine (BTA), ethylamine (ETA), isopropylamine (IPA), ethylenediamine (EDA), ethanol (ETL), ethanol-ammonium (ETL-AM) and no template (NT)) under hydrothermal conditions. The samples were characterized by XRD, SEM, XRF, NH3-TPD and BET surface area measurements in order to understand the template effects and the differences of the ZSM-5 samples. The synthesis of ZSM-5 with organic templates was relatively easier than those with inorganic templates and without template. SEM results revealed that ZSM-5 synthesized with different templates had different morphology and particle size. The Si/Al ratio and BET specific surface area of the sample with ethanol as template was the lowest. NH3-TPD results showed that the sample synthesized without template had fewer strong acid sites than others. n-Hexane cracking reaction was carried out over the samples to evaluate the catalytic properties. All ZSM-5 zeolites were effective in n-hexane cracking reaction, especially for the sample synthesized without template. (C) 2004 Elsevier B.V. All rights reserved.

草甸白浆土水解酶活性和动力学特性对保护性耕作方式的响应

在田间试验条件下,以传统耕作(CT)为对照,研究了免耕(NT)和少耕(RT)对黑龙江省八五三农场草甸白浆土土壤与碳、氮、磷、硫元素转化相关的九种水解酶(α—葡糖苷酶、β—葡糖苷酶、α—半乳糖苷酶、β半乳糖苷酶活性脲酶、蛋白酶、磷酸单酯酶、磷酸二酯酶和芳基硫酸醅酶)的影响,同时研究了保护性耕作对土壤脲酶、磷酸单酯酶、磷酸二酯酶、芳基硫酸酯酶及β—葡糖苷酶动力学特性的影响。结果表明,保护性耕作(RT,NT)降低土壤pH值,提高土壤养分(有效氮:42.0%,21.6%;总碳:26.6%,12.2%;总氮:16.6%,15.8%)含量,提高了土壤α—半乳糖苷酶、β—半乳糖苷酶和α—葡糖苷酶、蛋白酶活性活性,但脲酶活性却降低,参与土壤磷素及硫素转化的磷酸单酯酶、磷酸二酯酶和芳基硫酸酯酶活性差异不显著,传统耕作处理(CT)与免耕(NT)处理土壤中芳基硫酸酯酶活性著高于少耕(MT)处理。土壤水解酶动力学参数对耕作方式的反应与表观活性对耕作方式的反应不一致,草甸白浆土短期(三年)保护性耕作已经体现了一定程度的土壤改良效果。

干旱和复水条件下转基因甘薯的光合特性

以转铜锌超氧化物歧化酶(Cu/Zn SOD)和抗坏血酸过氧化物酶(APX)基因甘薯(TS)及未转基因甘薯(NT)为实验材料,研究在旱后复水条件下转基因甘薯及未转基因甘薯抗氧化酶活性和光合特性变化。结果显示,连续36 h胁迫条件下,TS和NT的SOD活性都先降低后升高,但TS的SOD活性始终高于NT。胁迫至24 h时,TS的SOD活性约为NT的1.2倍,复水后二者SOD活性都下降。持续胁迫,TS的APX活性先升高后降低,NT与之相反,复水后TS和NT的APX活性都是先升高后降低,复水12 h,TS的APX活性是NT的1.5倍。水分胁迫条件下TS的膜质受伤害程度要轻于NT,胁迫24 h,复水12 h,NT的MDA含量均约为TS的1.2倍。胁迫12 h,TS和NT净光合速率都下降,继续胁迫,TS净光合速率开始上升,NT几乎保持不变,胁迫36 h,TS的净光合速率约为NT的1.5倍。复水后二者净光合速率都开始上升,复水12 h,TS净光合速率约为NT的3倍。胁迫时TS、NT胞间CO2浓度(Ci)都逐渐增大,胁迫36 h时NT胞间CO2浓度显著高于TS,是其1.4倍。实验结果表明,同时转入SOD、APX抗氧化基因后,在...

猕猴桃优质高效管理专家系统的研制与应用

猕猴桃优质高效管理专家系统是在 Window NT(4.0 )环境下开发的 ,适用于 Internet的一个人工智能软件系统 ,该系统由苗木繁殖技术、高接换种技术、整形修剪技术、疏蕾授粉疏果技术、果园生草技术、优化施肥技术、节水灌溉技术、病虫害防治技术、冻害预防技术、果品保鲜贮藏技术和系统维护等 14个子系统组成 ,介绍了系统结构及其实现技术。应用示范结果表明 ,该系统指导的 6 6 .6 7hm2示范果园年增产值 90 0 0元 / hm2 ,推广辐射的 6 70 0 hm2果园年增产值 2 840万元。

Titanium and zirconium complexes with aminoiminophosphorane ligands

A series of titanium and zirconium complexes based on aminoiminophosphorane ligands [Ph2P(Nt-Bu)(NR)](2)MCl2 (4, M = Ti, R = Ph; 5, M = Zr, R = Ph; 6, M = Ti, R = SiMe3; 7, M = Zr, R = SiMe3) have been synthesized by the reaction of the ligands with TiCl4 and ZrCl4. The structure of complex 4 has been determined by X-ray crystallography. The observed very weak interaction between Ti and P suggests partial pi-electron delocalization through both Ti and P. The complexes 4-7 are inactive for ethylene polymerization in the presence of modified methylaluminoxane (MMAO) or i-Bu3Al-Ph3CB(C6F5)(4) under atmospheric pressure, and is probably the result of low monomer ethylene concentration and steric congestion around the central metal.

热致液晶性序列嵌段共聚酯的多重热转变

本文利用DSC研究了热致液晶性序列嵌段共聚酯在不同升温速率时的多重熔融转变,和不同冷却速率对结晶与熔融过程的影响。发现T_(kn)和T_(ni)与热历史、升温速率及成形条件均无关,T_(kn)=425.86K,T_(nt)=480.60K;而T_(in)与T_(nk)随冷却速率增大而降低。

Interaction between Thymidylate Synthase and Its Cognate mRNA in Zebrafish Embryos

Thymidylate synthase (TS), which catalyzes the de novo synthesis of dUMP, is an important target for cancer therapy. In this report, the effects of 5-fluorouracil (5-FU) and ZD1694 on the regulation of TS gene expression were evaluated in zebrafish embryos. Our results revealed that the expression of TS was increased by about six-fold when embryos were treated with 1.0 mu M 5-FU and there was a greater than 10-fold increase in the TS protein level after treatment with 0.4 mu M ZD1694. Northern blot analysis confirmed that expression of TS mRNA was identical in treated or untreated embryos. Gel shift and immunoprecipitation assays revealed that zebrafish TS was specifically bound with its cognate mRNA in vitro and in vivo. We identified a 20 nt RNA sequence, TS:N20, localized to the 5'-UTR of TS mRNA, which corresponded to nt 13-32; TS:N20 bound to the TS protein with an affinity similar to that of the full-length TS mRNA. The MFold program predicted that TS:N20 formed a stable stem-loop structure similar to that of the cis-acting element found in human TS mRNA. Variant RNAs with either a deletion or mutation in the core motif of TS:N20 were unable to bind to the TS protein. In vitro translation experiments, using the rabbit lysate system, confirmed that zebrafish TS mRNA translation was significantly repressed when an excess amount of TS protein was included in the system. Additionally, a TS stability experiment confirmed that treatment of zebrafish embryos with 5-FU could increase the TS stability significantly, and the half life of TS protein was about 2.7 times longer than in untreated embryos. Our study revealed a structural requirement for the interaction of TS RNA with TS protein. These findings also demonstrated that the increase in TS protein induced by 5-FU occurs at the post-transcriptional level and that increased stability and translation efficiency both contributed to the increase in TS protein levels induced by TS inhibitors.