Florescimento e frutificação em lichieiras

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Variação espaço temporal da comunidade zooplanctônica em viveiros de cultivo de camarão branco, Litopenaeus vannamei (Boone, 1931), no município Curuçá, Pará-Brasil

Ao longo das últimas décadas, a carcinicultura vem apresentando um grande crescimento em diversas partes do mundo, com o Brasil seguindo esta tendência mundial (FAO, 2004). Nesta atividade três espécies de camarão têm se destacado como as mais cultivadas, sendo elas Penaeus monodon (Fabricius, 1798), Fenneropenaeus chinensis (Osbeck, 1765) e Litopenaeus vannamei (Boone, 1931), responsáveis por cerca de 80% da produção mundial (FAO, 2004). No Brasil L. vannamei é a espécie mais cultivada, com a produção brasileira correspondendo a 5% da produção mundial (FAO, 2004). L. vannamei é uma espécie marinha originária do Oceano Pacífico, distribuída do México ao Peru. Por ser eurihalino, este camarão pode se adaptar às mais diversas condições de cultivo, desde águas salgadas até de menores salinidades (BRAY et al., 1993; PONCE-PALAFOX et al., 1997), característica que tem aumentado o interesse dos produtores. Embora seja exótica no Brasil, L. vannamei, mostra maior resistência à variação de temperatura e salinidade do que outros camarões peneídeos nativos (BRITO et al., 2000). O alimento do camarão e as estratégias de seu fornecimento têm merecido uma atenção especial do setor, gerando novas técnicas ou seu aperfeiçoamento. A ração nos sistemas de cultivo intensivo e semi-intensivo, por exemplo, é responsável por 50-60% dos custos totais de produção, demonstrando a importância de novas estratégias para minimizar sue uso. O aumento da biomassa do plâncton (alimento natural), e conseqüentemente, da cadeia alimentar, reduz os custos com a alimentação suplementar, influenciando diretamente os custos finais de produção (AVAULT, 2003). Segundo Nunes (1995), o incremento da produtividade natural é tão importante quanto o uso de uma ração nutricionalmente completa e bem balanceada. Logo após a introdução nos viveiros de cultivo, a base da alimentação de L. vannamei é composta, em parte, pelo alimento natural disponível (NUNES et al. 1997; MARTINEZ-CORDOVA et al. 1997; ROTHLISBERG, 1998) complementada com ração comercial. Martinez-Cordova et al. (2002) mostraram que as concentrações de clorofila ‘a’ diminuem cerca de 50% do início ao fim do cultivo, provavelmente devido a pastagem pelo zooplâncton e por alguns invertebrados bentônicos. Além da importância do zooplâncton como alimento para as pós-larvas de camarão nos viveiros de engorda, o uso destes organismos (principalmente copépodes) como alimento vivo na aqüicultura marinha vem recebendo grande atenção nos últimos anos (DELBARE et al. 1996). Tal fato ocorre por serem ricos em fosfolipídios, ácidos graxos essenciais altamente insaturados e antioxidantes naturais, sendo nutricionalmente superiores aos rotíferos e aos náuplios de artemia, comumente usados na larvicultura marinha (SARGENT et al. 1997, STOTTRUP e NOSKER, 1997) promovendo o sucesso as larviculturas de camarão (PAYNE et al. 1998; SCHIPP et al. 1999; PAYNE e RIPPINGALE, 2000). Desta forma, estudos sobre o cultivo intensivo de camarões marinhos que enfoquem a composição da comunidade planctônica, as variáveis bióticas e abióticas no sistema, e a característica dos efluentes gerados, são de grande importância. Assim, os resultados obtidos podem incrementar a produtividade aquática no cultivo, alem de fornecer subsídios para pesquisas posteriores de avaliação e mitigação dos impactos ambientais causados por esta atividade.

Influência de temperatura, anelamento e reguladores de crescimento, sobre a floração e frutificação de lichieiras

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Aplicação de métodos combinados na conservação da qualidade de lichias ‘Bengal’

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ácido cítrico e quitosana na conservação de lichias 'Bengal'

The aim of this study was to evaluate citric acid application with or without chitosan on the maintenance of the quality of litchi 'Bengal'. It was used litchi on mature maturation stage. After selection, fruits were immersed for one minute on the solution of citric acid and chitosan: [1]Control - without immersion; [2] citric acid 300g L-1, [3] citric acid 300 g L-1 + chitosan 0,3%, [4]citric acid 300 g L-1 + chitosan 0,6%, [5] citric acid 600 g L-1, [6] citric acid 600 g L-1 + chitosan 0,3%, [7] citric acid 600 g L-1 + chitosan 0,6%. Chitosan of low molecular weight and deacetylation degree of 75,58% (Sigma - Aldrich®) were used. After immersion, fruits were put to drain the excess of solution. Then, they were stored in cool chamber at 5ºC, previously sanitized, for 20 days. The experiment was conducted following a completely randomized design, with a factorial scheme composed by seven coating solutions and five sampling dates. Every five days were evaluated: loss of fresh mass of fruits; color; content of anthocyanins and the activity of peel enzymes polyphenoloxidase and peroxidase. It was verified that the solution of citric acid 600 g L-1, associated or not to chitosan, and the combination of chitosan 0,3% to 300 g L-1 of citric acid were the most efficient to maintain the quality of litchi 'Bengal' for 20 days at 5ºC.

A Method to Estimate the Population Level of Aceria litchii (Prostigmata: Eriophyidae) and a Study of the Population Dynamics of This Species and Its Predators on Litchi Trees in Southern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Development of a Methodology and Evaluation of Pesticides Against Aceria litchii and Its Predator Phytoseius intermedius (Acari: Eriophyidae, Phytoseiidae)

The litchi erineum mite, Aceria litchii (Keifer), is the major pest of litchi, Litchi chinensis Sonnerat (Sapindaceae). This study evaluated the effect of 11 pesticides on the survival of A. litchii as well as on the survival and reproduction of Phytoseius intermedius Evans& McFarlane, the predator most found in association with it in Brazil. The containment of A. litchii in small petri dishes whose bottoms were covered with a solidified paste made of a mixture of gypsum and activated charcoal (9: 1 in volume), kept humid, was shown to be adequate for this type of study. For the evaluation of the effect of pesticides on A. litchii, mites leaving the erinia from the pieces of litchi leaves (removed from the plants approximate to 24 h earlier) were sprayed under a Potter tower and immediately transferred to the 2.5-cm-diameter petri dishes. After 2, 12, 24, and 48 h of the application, the dishes were examined to evaluate the mite survival. The four pesticides causing the highest levels A. litchii mortality, as well as azadirachtin, were tested for the effect on P. intermedius. For this test, experimental units consisted of discs of uninfested litchi leaves also sprayed under a Potter tower before introducing the predators. Survival and oviposition of the predator were evaluated every 24 h for 5 consecutive days; viability of the eggs laid was also evaluated. Highest mortality of A. litchii occurred with the application of fenpyroximate, sulfur, abamectin, and hexythiazox. Azadirachtin was considered moderately harmful to the predator P. intermedius, whereas other pesticides were classified as harmful. Despite the low efficiency of azadirachtin in the control of the pest, its relative selectivity to P. intermedius would encourage the evaluation on field condition, especially for use in organic production systems.

Jasmonates and abscisic acid influence fruit ripening and plant water use: practical, physiological and morphological aspects

The aim of the present thesis was to better understand the physiological role of the phytohormones jasmonates (JAs) and abscisic acid (ABA) during fruit ripening in prospect of a possible field application of JAs and ABA to improve fruit yield and quality. In particular, the effects of exogenous application of these substances at different fruit developmental stages and under different experimental conditions were evaluated. Some aspects of the water relations upon ABA treatment were also analysed. Three fruit species, peach (Prunus persica L. Batsch), golden (Actinidia chinensis) and green kiwifruit (Actinidia deliciosa), and several of their cvs, were used for the trials. Different experimental models were adopted: fruits in planta, detached fruit, detached branches with fruit, girdled branches and micropropagated plants. The work was structured into four sets of experiments as follows: (i) Pre-harvest methyl jasmonate (MJ) application was performed at S3/S4 transition under field conditions in Redhaven peach; ethylene production, ripening index, fruit quality and shelf-life were assessed showing that MJ-treated fruit were firmer and thus less ripe than controls as confirmed by the Index of Absorbance Difference (IAD), but exhibited a shorter shelf-life due to an increase in ethylene production. Moreover, the time course of the expression of ethylene-, auxin- and other ripening-related genes was determined. Ripening-related ACO1 and ACS1 transcript accumulation was inhibited though transiently by MJ, and gene expression of the ethylene receptor ETR2 and of the ethylene-related transcription factor ERF2 was also altered. The time course of the expression of several auxin-related genes was strongly affected by MJ suggesting an increase in auxin biosynthesis, altered auxin conjugation and release as well as perception and transport; the need for a correct ethylene/auxin balance during ripening was confirmed. (ii) Pre- and post-harvest ABA applications were carried out under field conditions in Flaminia and O’Henry peach and Stark Red Gold nectarine fruit; ethylene production, ripening index, fruit quality and shelf-life were assessed. Results show that pre-harvest ABA applications increase fruit size and skin color intensity. Also post-harvest ABA treatments alter ripening-related parameters; in particular, while ethylene production is impaired in ABA-treated fruit soluble solids concentration (SSC) is enhanced. Following field ABA applications stem water potential was modified since ABA-treated peach trees retain more water. (iii) Pre- and post-harvest ABA and PDJ treatments were carried out in both kiwifruit species under field conditions at different fruit developmental stages and in post-harvest. Ripening index, fruit quality, plant transpiration, photosynthesis and stomatal conductance were assessed. Pre-harvest treatments enhance SSC in the two cvs and flesh color development in golden kiwifruit. Post-harvest applications of either ABA or ABA plus PDJ lead to increased SSC. In addition, ABA reduces gas exchanges in A. deliciosa. (iv) Spray, drench and dipping ABA treatments were performed in micropropagated peach plants and in peach and nectarine detached branches; plant water use and transpiration, biomass production and fruit dehydration were determined. In both plants and branches ABA significantly reduces water use and fruit dehydration. No negative effects on biomass production were detected. The present information, mainly arising from plant growth regulator application in a field environment, where plants have to cope with multiple biotic and abiotic stresses, may implement the perspectives for the use of these substances in the control of fruit ripening.

Detection and molecular characterization of viruses infecting Actinidia spp.

Kiwifruit (genus Actinidia) is an important horticultural crop grown in the temperate regions. The four world’s largest producers are China, Italy, New Zealand and Chile. More than 50 species are recognized in the genus but the principal species in cultivation are A. deliciosa and A. chinensis. In Italy, as well as in many other countries, the kiwifruit crop has been considered to be relatively disease free and then no certification system for this species has been developed to regulate importation of propagation plant material in the European Union. During the last years a number of fungal and bacterial diseases have been recorded such as Botrytis cinerea and Pseudomonas syringae pv. actinidiae. Since 2003, several viruses and virus-like diseases have been identified and more recent studies demonstrated that Actinidia spp can be infected by a wide range of viral agents. In collaboration with the University of Auckland we have been detected thirteen different viral species on kiwifruit plants. During the three years of my PhD I worked on the characterization of Cucumber mosaic virus (CMV) and Pelargonium zonate spot virus (PZSV). The determination of causal agents has been based on host range, symptom expression in the test plant species and morphological properties of the virus particles using transmission electron microscopy (TEM) and using specific oligonucleotide primers in reverse transcription-polymerase chain reaction (RT-PCR). Both viruses induced several symptoms on kiwifruit plants. Moreover with new technologies such as high-throughput sequencing we detected additional viruses, a new member of the family Closteroviridae and a new member of the family Totiviridae. Taking together all results of my studies it is clear that, in order to minimize the risk of serious viral disease in kiwifruit, it is vital to use virus-free propagation material in order to prevent the spread of these viruses.

A 170 year spring phenology index of plants in eastern China

Extending phenological records into the past is essential for the understanding of past ecological change and evaluating the effects of climate change on ecosystems. A growing body of historical phenological information is now available for Europe, North America, and Asia. In East Asia, long-term phenological series are still relatively scarce. This study extracted plant phenological observations from old diaries in the period 1834–1962. A spring phenology index (SPI) for the modern period (1963–2009) was defined as the mean flowering time of three shrubs (first flowering of Amygdalus davidiana and Cercis chinensis, 50% of full flowering of Paeonia suffruticosa) according to the data availability. Applying calibrated transfer functions from the modern period to the historical data, we reconstructed a continuous SPI time series across eastern China from 1834 to 2009. In the recent 30 years, the SPI is 2.1–6.3 days earlier than during any other consecutive 30 year period before 1970. A moving linear trend analysis shows that the advancing trend of SPI over the past three decades reaches upward of 4.1 d/decade, which exceeds all previously observed trends in the past 30 year period. In addition, the SPI series correlates significantly with spring (February to April) temperatures in the study area, with an increase in spring temperature of 1°C inducing an earlier SPI by 3.1 days. These shifts of SPI provide important information regarding regional vegetation-climate relationships, and they are helpful to assess long term of climate change impacts on biophysical systems and biodiversity.

Molluscan biostratigraphy of the Miocene and Chatt in the southern Herzogtum Lauenburg (Schleswig-Holstein)

A study was made of the marine molluscan fauna from 12 borings in the Schwarzenbek area. In the fossil rich facies underlying the 'Braunkohlensande', the Neochatt and Vierland faunal sequences could be described and used to define the Oligocene/Miocene boundary. The Neochatt, defined by Pectinidae, seems to be more closely related to the Miocene than previously thought. Nevertheless, a sufficient number of additional molluscan species are present for placing the Neochatt/Vierland boundary. Overlying the Braunkohlensande, the sandy Reinbek fauna as well as Glimmerton faunas of the Reinbek and Langenfelde stages could be described.

Fossil record of Mollusca from Neogene sediments in Schleswig-Holstein, Germany

Über die Verbreitung, Gliederung und Ausbildung des Jungtertiärs im westlichen Schleswig-Holstein war bisher nicht viel bekannt. Am besten bearbeitet sind die glazial gestauchten Schollen von Morsum/Sylt. Eine Aufzählung erbohrter Miozänvorkommen mit nicht immer überzeugender Begründung lieferte H.-L. HECK 1935. S. THIELE (1941) hat die ihm bekannten Vorkommen hauptsächlich nach faziellen und petrographischen Gesichtspunkten bearbeitet. Er erkannte richtig die Stellung der Braunkohlensande. Die angekündigte palaeontologische Bearbeitung ist nicht erschienen. Eine allgemeine Übersicht über die Entwicklung des Jungtertiärs bringen W. WOLFE und H.-L. HECK 1949. W. HINSCH lieferte wertvolle Beiträge zur Molluskenfauna und zur Gliederung des Miozäns (1952, 1955). Über neue Vorkommen von Braunkohlen-Sanden berichtete E. DITTMER(1 956), eine erste Übersicht über neue Vorkommen der Hemmoorer Stufe gab derselbe Verfasser 1957.

Miocene molluscan biostratigraphy in Schleswig-Holstein, north Germany

Vierlandian, Behrendorfian (Lower Hemmoorian), Oxlundian (Upper Hemmoorian), Lower and Upper Reinbekian, Langenfeldian and Gramian stages could be proved by evaluation of marine molluscan faunas. The diachrone base of 'Braunkohlensande' is demonstrated by underlying Vierlandian mica clay in the E, and by Hemmoorian substages more to the W, at last the fluviatile facies is replaced completely by euhaline to brachyhaline sandy to silty sediments. Brachyhaline effects in adjacent environments make possible an approximate dating on fluviatile sedimentation. The widest extension of 'Braunkohlensand' is during upper Oxlundian, whilst slightly brachyhaline Katzheide beds, defined in this paper to be of Lower Reinbekian age, indicate a limit of 'Braunkohlensande' more to the E. Winnert-fauna was found to be a mixture of Oxlundian and Langenfeldian; the overlying lignitic sands belong to the Kaolinsand group. Upper mica clay overlying Miocene Braunkohlensande can be divided into beds of Upper Reinbekian, Langenfeldian and Gramian ages.