Caracterização molecular do vírus Melao cepas BE AR 633512 e BE AR 8033 (Bunyaviridae, Orthobunyavirus) e infecção experimental em hamsters dourados (Mesocricetus auratus)

As cepas do Virus Melao (VMEL), BE AR 8033 e BE AR 633512 foram isoladas de mosquitos Ochlerotatus (Ochlerotatus) scapularis, em Belém- PA (1955) e Alta Floresta do Oeste- RO (2000), respectivamente. Este trabalho teve como objetivo caracterizar molecularmente as cepas BE AR 633512 e BE AR 8033 e realizar estudos histopatológicos, bioquímicos e imunológicos comparativos em hamsters dourados (Mesocricetus auratus). Hamsters mostraram suscetibilidade às cepas do VMEL. A viremia em hamsters para BE AR 633512 ocorreu do 3º ao 6º dias pós-infecção (dpi.), e para a cepa BE AR 8033 ocorreu no 2º dpi. Anticorpos neutralizantes para ambas as cepas foram detectados a partir de 5 dpi., e se mantiveram até 30 dpi. As cepas testadas alteraram os marcadores bioquímicos AST, ALT e uréia, enquanto que a creatinina só apresentou alteração estatisticamente significante nos animais infectados com a cepa viral BE AR 633512, em comparação aos animais controles não infectados. Alterações histopatológicas foram observadas no SNC, fígado, rim e baço dos hamsters infectados pelas cepas do VMEL, sendo a infecção nesses órgãos confirmada por imunohistoquímica. A cepa BE AR 633512 foi mais virulenta e patogênica para hamsters que a cepa BE AR 8033. A análise genética dos genes N, Gn e Gc revelou que para os genes N e Gn, a cepa BE AR 8033 e do protótipo VMEL (TRVL 9375) são mais geneticamente relacionados. Para o gene Gc, a cepa BE AR 8033 é mais relacionada com a cepa BE AR 633512, sendo que esta última cepa apresentou maior variabilidade genética, principalmente no gene Gn com várias substituições de aminoácidos, mas as mutações no gene Gc provavelmente foram responsáveis pelo aumento da virulência e patogenicidade em hamsters.

Verificação da proporção áurea em medidas cefalométricas laterais de indivíduos com síndrome de Down

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Biomarcadores renais de lesão glomerular em pacientes submetidos à anestisia para cirurgia arterial

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Parâmetros vocais perceptivo-auditivos e acústicos em crianças com nódulos vocais

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Estudo comparativo das representações sociais sobre diabetes mellitus e pé diabético

The study aimed to investigate social representations of the terms diabetes and diabetic foot in different populations. Participants were divided into groups: diabetics (GD); non- diabetics (GN); and non-diabetic healthcare professionals (GP). Personal data were collected, and subjects answered two questions that were expected to evoke five words that came to mind when they thought of diabetes and then diabetic foot. The evoked material was analyzed with the software Ensemble de Programmes Permettant l'Analyse dês Èvocations. A total of 161 subjects participated, including GD (n = 72) with a mean age of 56.12 ± 5.49 years; GN (n = 38) with a mean age of 54.29 ± 7.91 years; and GP (n = 51) with 34.95 ± 7.52 years. The term diabetes evoked 297 words in GD, 172 in GN, and 235 words in GP. The term diabetic foot evoked 180 words in GD, 90 in GN, and 236 in GP. The groups proved to be anxious for more information, thus confirming the need for awareness-raising and educational programs on diabetes, covering comprehensive issues concerning the disease.

Estudo comparativo de dois programas de computador (Dolphin Imaging e Nemotec) versus o método manual utilizados para análises cefalométricas de pacientes com deformidade dento-facial padrão II

A-Nperp, Pg-Nperp e mandibular plane). Thirty patients (male or female), with age varying from 19 to 48 years old were analyzed. All patients were diagnosted with mandibular retrognathism and submitted to bilateral sagital mandibular advancement. All tracings were performed in lateral cephalometric radiographs obtained one week before surgery. The intraclass correlation coefficient analysis (ICC), the t test adjusted for Tukey-Kramer test and Schuirmann test were applied. The results showed that the manual tracings and Nemotec® tracing had excellent reliabilities for all measures (ICC > 0.98). The Dolphin Imaging® showed low reliability in anterior facial height (value of ICC = 0.70), Co-A (value of ICC = 0.47) and Co-Gn (ICC value = 0.49). In A-Nperp, Pg-Nperp, Mandibular plan, SNA and SNB there were no differences between the 3 tracings (p > 0.05), for the anterior facial height measures differences were found between the Dolphin Imaging® and Nemotec® tracings, but no differences were observed as compared to the manual tracing (p > 0.05), in Co-A and Co-Gn measures t Dolphin Imaging® presented a significantly lower mean than the other methods (p > 0.05). The manual tracings were equivalent in 6 of 8 measures (A-Nperp, Pg-Nperp, md Plan, SNA, SNB and Co-A), there was no equivalence between the methods in the anterior facial height and Co-Gn measures (p < 0.01). The Dolphin Imaging® method was not equivalent in any of the 8 measures. It was concluded that in the manual tracing only the Co- Gn, Pg-Nperp and SNB measures confirmed the diagnosis of mandibular retrognathism, and the Nemotec® software showed better results than the Dolphin Imaging® software.

Long-term skeletal and dental effects and treatment timing for functional appliances in Class II malocclusion

Objective: To analyze the long-term skeletal and dentoalveolar effects and to evaluate treatment timing of Class II treatment with functional appliances followed by fixed appliances.Materials and Methods: A group of 40 patients (22 females and 18 males) with Class II malocclusion consecutively treated either with a Bionator or an Activator followed by fixed appliances was compared with a control group of 20 subjects (9 females and 11 males) with untreated Class II malocclusion. Lateral cephalograms were available at the start of treatment (mean age 10 years), end of treatment with functional appliances (mean age 12 years), and long-term observation (mean age 18.6 years). The treated sample also was divided into two groups according to skeletal maturity. The early-treatment group was composed of 20 subjects (12 females and 8 males) treated before puberty, while the late-treatment group included 20 subjects (10 females and 10 males) treated at puberty. Statistical comparisons were performed with analysis of variance followed by Tukey's post hoc tests.Results: Significant long-term mandibular changes (Co-Gn) in the treated group (3.6 mm over the controls) were associated with improvements in the skeletal sagittal intermaxillary relationship, overjet, and molar relationship (similar to 3.0-3.5 mm). Treatment during the pubertal peak was able to produce significantly greater increases in total mandibular length (4.3 mm) and mandibular ramus height (3.1 mm) associated with a significant advancement of the bony chin (3.9 mm) when compared with treatment before puberty.Conclusion: Treatment of Class II malocclusion with functional appliances appears to be more effective at puberty. (Angle Orthod. 2013;83:334-340.)

Influência da dexmedetomidina/atropina sobre as alterações da variação de pulso induzidas pela hemorragia seguida por reposição volêmica em cães anestesiados com isoflurano

Pós-graduação em Anestesiologia - FMB

Gait stability in diabetic peripheral neuropathy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determinação de adenosina deaminase, fator de necrose tumoral-alfa e interleucina-1 em gestantes portadoras de pré-eclâmpsia

Preeclampsia is a specific disorder of pregnancy, characterized by arterial hypertension and proteinuria detected after 20 weeks of gestation. This pathology is associated with hyperuricemia, higher levels of pro-inflammatory cytokines, enhanced leukocyte activation and oxidative stress. Adenosine deaminase (ADA) is an enzyme present in all human tissues and, it is involved with the maturation of the immune system. Although its function is not fully understood, ADA is considered an indicator of cellular inflammation and, its increased serum concentration is observed in inflammatory diseases, such as tuberculosis and rheumatoid arthritis. This study aimed to assess serum ADA levels in preeclamptic patients (PE) compared with normotensive pregnant (NT) and non-pregnant women (NP), and to correlate these values with TNF-α and IL-1β production. Ninety pregnant women were included: 60 were pre-eclamptic and 30 were normotensive matched for gestational age. As control group 20 healthy non-pregnant women matched with pregnant for age were included. Peripheral blood mononuclear cells (PMMC) obtained from the three groups studied were cultured with or without lipopolysaccharide (LPS) for 18h at 37oC, and TNF-α and IL-1β production was assessed in the supernatant of cultures by enzyme immunoassay (ELISA). ADA plasmatic concentration was determined by colorimetric method. The results show that ADA plasma levels were significantly higher in PE group compared with NT and NP groups. A positive correlation between ADA and uric acid levels was detected in preeclamptic women. There was no significant difference in relation to ADA levels when PE patients were classified in early and late-onset PE. The endogenous production of IL-1β and TNF-α by PBMC was significantly higher in PE group than in NT and NP women, showing the activation state of these cells in PE. LPS induced...(Complete abstract click electronic access below)

Efeito da silibinina sobre o fator de transcrição nuclear-kB e produção de citocinas inflamatórias por células mononucleares de gestantes portadoras de pré-eclâmpsia

Pre-eclampsia (PE) is a complication of human pregnancy characterized by hypertension and proteinuria after 20 weeks of gestation. Its incidence varies from 5% to 7% of pregnancies and is a major cause of morbidity and maternal and fetal mortality. This is a multisystemic disease, with focus on vascular dysfunction and is closely related to the exacerbated activation of the immune system. In addition to increased activation of monocytes and granulocytes, there is an elevated production of proinflammatory cytokines in pregnant women with PE. The nuclear transcription factor-kB (NF-kB) is present in the cells of the immune system and is responsible for transcription of genes related to inflammation. Whereas the PE is associated with intense inflammatory response, the use of substances modulating the activity of NF-kB factor could be useful in alleviating the inflammation present in these patients. Silibinin is the main component of silymarin, a polyphenolic extract obtained from fruits and seeds of Sylibum marianum with potent hepatoprotective, anti-inflammatory and anti-fibrotic activities. The silibinin mechanism of action includes the ability to inhibit NF-kB activation and, consequently, its migration to the nucleus. The objective of this study was to assess whether silibinin modulates the activity of NF-kB and the production of inflammatory cytokines in mononuclear cells of patients with PE. We evaluated 34 pregnant women with PE, 20 normotensive pregnant women (GN) and 15 non-pregnant women (NG). Peripheral blood mononuclear cells (PBMC) were obtained from those groups of women and cultured in the presence or absence of silibinin (5 uM or 50 uM) and stimulated or not with lipopolysaccharide (LPS) for 18 h to obtain supernatant for determination of tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1β) by enzyme immunoassay (ELISA). The cells were...(Complete abstract click electronic access below)

Estudo sobre uma turbina a gás instalada em uma refinaria de petróleo

The gas turbine (GT) is known to have: low cost of capital over the amount of energy, high flexibility, high reliability, short delivery time, commissioning and commercial operation at the beginning and quick departure. The gas turbine is also recognized for its superior environmental performance, manifested in air pollution containment and reducing greenhouse gases (Mahi, 1994). Gas turbines in simple cycle mode (SC) have long been used by utilities to limited power generation peak. In addition, manufacturing facilities use gas turbines for power generation units on site, often in combination with the process of heat production, such as hot water and steam process. In recent years, the performance of industrial gas turbines has been improved due to significant investments in research and development, in terms of fuel to electricity conversion efficiency, plant capacity, availability and reliability. The greater availability of energy resources such as natural gas (NG), the significant reduction of capital costs and the introduction of advanced cycles, have also been a success factor for the increased use of gas turbines to load applications base (Poulikas, 2004). Open Cycle Gas Turbine with a greater degree of heat to the atmosphere may alternatively be used to produce additional electricity using a steam cycle, or to compose a cogeneration process. The combined cycle (CC) uses the heat from the gas turbine exhaust gas to increase the power output and increase the overall efficiency of more than 50% second (Najjar, 2001). The initial discovery of these cycles in the commercial power generation market was possible due to the development of the gas turbine. Only from the 1970s that gas turbine inlet temperature and therefore the exhaust gas temperature was sufficiently high to allow a better efficiency in the combined cycle ... (Complete Abstract click electronic access below)

Avaliação do balanço nitrogenado e aparecimento de nitrogênio uréico como marcadores de catabolismo, infecção e mortalidade em pacientes com lesão renal aguda em diálise

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Efeitos da corrente elétrica contínua e do sulfato de zinco administrado pela iontoforese transdérmica sobre a cicatrização da pele de ratos diabéticos induzidos pela aloxana: análise morfológica e ultraestrutural

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise das alterações morfológicas e ultraestruturais do fígado de ratos diabéticos induzidos pela aloxana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)