Atividade in vitro da fosfolipases A2 e da fração peptídica extraídas do veneno de Crotalus durissus terrificus sobre formas amastogotas e promastigotas de Leishmania (L) infantum chagasi

Pós-graduação em Doenças Tropicais - FMB

Controle autonômico do coração e fração de ejeção na fase crônica do acidente vascular encefálico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do oxigênio intersticial no módulo de elasticidade de Ligas Ti-15Mo-xZr(x=5,10,15%p)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Preparação e caracterização estrutural, mecânica e anelástica de ligas do sistema Ti-Ta contendo oxigênio intersticial, para uso biomédico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de fração volumétrica de austenita retida e martensita em aço trip 800 utilizando três métodos diferentes

In the second half of the last century the automobile industries suffered from the petroleum crisis caused mainly by the wars in the Middle East. These crises led the automakers rethink their vehicles. One of the most important events after that was the adoption of new steels by the industry. One example is the TRIP steels (Transformationinduced plasticity). It is known that the macroscopic behavior of a material is strongly dependent on its microstructure and therefore the quantitative metallography is important to understand and relate the material properties to its microstructure. In this work, different specimens of TRIP steels were etched using LePera reagent. The obtained images were analyzed using digital processing. Using the ImageJ software the methods threshold and watershed were studied as well as a comparison with the ASTM E562 standard. The methods were compared and finally the morphological characteristics and volumetric fraction of the retained austenite and martensite phases were analyzed. The results showed that the threshold led to a higher number of identified grains with lower mean area and total area fraction than the watershed method and ASTM standard

Susceptibilidade do Staphylococcus aureus à terapia fotodinâmica (PDT)

A terapia fotodinâmica (PDT), de maneira simplificada pode ser aplicada utilizando um fotossensibilizador (FS) e uma fonte de luz com comprimento de onda específico, que combinados na presença do oxigênio produzem espécies citotóxicas que causam o dano celular. O objetivo deste estudo piloto foi verificar a susceptibilidade de Staphylococcus aureus (ATCC 25923) à PDT, empregando diferentes concentrações de azul de metileno. As amostras desta bactéria foram sensibilizadas com azul de metileno nas concentrações de 6,25 μg/mL; 12,5 μg/mL; 25,0 μg/mL; 50,0 μg/mL; 100,0 μg/mL e 200,0 μg/mL e expostas à luz LED vermelha (660nm) por 20 minutos. Após o tratamento de todos os grupos estudados a fração de sobrevivência foi calculada pela contagem das unidades formadoras de colônia (UFC). O resultado deste estudo mostrou que o fotossensibilizador no escuro foi tóxico (dark toxicity) nas concentrações de 100 e 200 μg/mL. Já no grupo onde utilizamos apenas a luz, não houve redução bacteriana significativa. Entretanto, o uso combinado do AM e da luz LED (Grupo PDT) mostrou que todas as concentrações testadas causaram uma redução significativa de S aureus. Desta forma concluímos que a terapia fotodinâmica antimicrobiana pode ser um tratamento alternativo para redução de Staphylococcus aureus.

Obtenção e caracterização de compósitos via RTM com alta fração de fibras de carbono processados à temperatura ambiente

After confirming the high specific mechanical properties of composite materials by scientific studies conducted over the last decades, one of the challenges of this new class of materials is the ability to achieve mass production at a more affordable cost, which has become indispensable. The Resin Transfer Molding (RTM) is an excellent method for manufacturing composite materials. Despite being a process widely used by international companies in the production of high performance structural composites, only a short time the national aviation industry has shown interest in implementing this type of processing to more complex structures and greater structural responsibility. In aeronautical projects, the reproducibility and the relative low cost of this process, several studies have been performed in Brazil for learning and perfecting this technique. This process is suitable for producing polymer components both simple as complex geometry, and allows to achieve consistent thickness, with high quality finish and without limiting range. Polymeric composite components for the high mechanical stress applications such as aircraft structures, satellites, etc., require a strict control of volume fractions of the composite constituents, beyond the knowledge of their mechanical and thermal properties. Therefore, in this experimental work degree study on the mechanical, thermal and of porosity composites processed by RTM processed characterization was performed. This characterization was performed targeting a possible aerospace application of this composite material. For the production of composites, process equipment (RTM RTM injector Radius 2100cc) was used. The processed carbono/epoxy composites were characterized via flexure tests mechanically and thermally analysis via DMA, DSC and TGA. To determine the volume fraction of fibers, the composite samples were analyzed via matrix digestion (ASTM D3171) ... (Complete abstract click electronic access below)

Avaliação do efeito da temperatura na deslignificação com o oxigênio nas propriedades do papel

The deslignification with oxygen, also denominated pre-O2, consists in a whitening stage, which consists of accomplishing an oxidation of the lignin, and remove it with the alkali, providing a larger earnings in the bleaching of the pulp. The pre-O2 is a process already very established, where a significant part of the cellulose of whitened short fiber produced nowadays suffers deslignification for this method. The conditions of work of this stage contemplate directly in the results of the deslignification level, in the physical, optical and mechanics properties of the pulp, and consequently of the paper, because this is important to know their effects fully. The main variables related to the control of this process are respectively: pressure and oxygen load, alkaline load, consistence, time and temperature, being this last variable was the study focus in this work. The objective of the work was to analyze the effect of the variation of the temperature in the oxygen whitening along every bleaching process of the pulp, refine and in the optical, physics and mechanics properties of the paper. The development of the work was based in four temperature levels (90, 95, 100 and 105°C) combined to two whitening sequences (OD0(E+P)D1P and OAHTD0(E+P)D1P). The results obtained in the oxygen deslignification stage indicated that the elevation of the temperature contemplated in increases of the whiteness, deslignification efficiency and in the viscosity loss allied to the reduction of the selectivity of the process. In the remaining of the whitening, the sequence that included the acid hydrolysis presented values slightly inferior of whiteness, kappa number, viscosity and yield in relation to the other sequence when compared with the samples of same temperatures. Already the physical tests showed that the sequence with acid stage amplifies the values of capillary... (Complete abstract click electronic access below)

Remoção de N-amoniacal em baixas concentrações de oxigênio dissolvido a partir do efluente de frigorífico bovino

This work aimed to develop a biological system for removal of ammonia nitrogen operating at low concentrations of dissolved oxygen. Thus, a biological upflow vertical reactor was built, in which the affluent pass through the support media until the top. Sludge from an anaerobic stabilization pond of a slaughterhouse unit in the city of Presidente Prudente - SP was used as inoculum. Initially the system operated in batch and afterwards in a continuous flow with different HRT. For feeding the reactor, an initial phases was adopted a synthetic culture media, described by Martins (2007), in order to establish the ideal conditions for the development of Anammox bacteria and subsequently, submitted to the system a slugde effluent of slaughterhouse. The results showed significant removal efficiency of N-NH4+, especially in the phase without recirculation of culture media, with an average of 71% removal, with the proportion of removal of N-NH4 +:N-NO2- average 1: 1,69. For the period of operation with effluent from the slaughterhouse, were not obtained satisfactory results, without confirmation of the proliferation of Anammox bacteria in the system, due to the high presences of organic matter in the same confirmed by high concentrations of COD

Efeito do treinamento físico aeróbio em hipertensos de meia idade e idosos nos valores de consumo máximo de oxigênio

The aim of this study was to investigate whether aerobic capacity (VO2max) would be modified by antihypertensive therapy in elderly and middle-age women after 12 weeks of exercise training. The volunteers were divided in two groups: normotensive (n=14) and hypertensive (n=14). Aerobic exercise was performed for 3 days/week, during 60 minutes, for 12 weeks with an intensity of 50-70% rest heart hate. Anthropometric parameters (weight and height), body fat index, % of fat mass, cardiopulmonary evaluation to calculated VO2max and a cardiovascular evaluation with blood pressure and rest heart hate were evaluated at baseline and after training program. At the end of study abdominal circumference and Borg scale were also evaluated. Our findings showed the aerobic program of 12 weeks was effective to reduce diastolic blood pressure in both groups. Systolic blood pressure was reduced only hypertensive group. No evidences were found the aerobic capacity was affected by hypertensive therapies. In conclusion, the aerobic program for 12 weeks was effective to reduce blood pressure and there was no influence of antihypertensive therapy on the aerobic capacity in this particular population.

Efeito da adição de oxigênio na produção de glicerol quinase pela levedura recombinante de Pichia pastoris

O glicerol é um importante subproduto de processos fermentativos. Em algumas bebidas alcoólicas, como vinhos e vodcas contribuem com seu sabor tornando-a aceitável para o consumidor. Com o incentivo na produção de biocombustíveis, a disponibilidade do glicerol deve aumentar, já que é o principal subproduto gerado na produção do Biodiesel. A sua presença em alguns alimentos, pode identificar presença de microrganismos, já que pode ser uma fonte de carbono para eles. Devido às inúmeras utilizações do glicerol para diversos tipos de indústrias, a dosagem do glicerol se torna cada vez mais importante e rotineira. Isso incentiva novas pesquisas de métodos de doseamento. A enzima GK é presente em vários microrganismos e converte o glicerol em glicerol-3-fosfato. Com a utilização da enzima Glicerol -3- fofato desidrogenase, o glicerol - 3 – fosfato é convertido a di-hidroxi-acetona fosfato com a produção de NADH. Este pode ser quantificado através do espectrofotômetro, tornando-se um rápido e eficiente método para doseamento. A levedura recombinante Pichia pastoris foi escolhida pois foi inserida no seu DNA, o Gene GUT1 que expressa a GK em Saccharomyces cerevisiae. Além disso, a expressão enzimática é extracelular, diminuindo etapas no processo de isolamento da enzima GK. O trabalho consistiu em otimizar a produção de GK expressa pela levedura recombinante Pichia pastoris por meio da adição de oxigênio no biorreator de fermentação em escala de 2,5 L. Os resultados do presente estudo indicam o ponto ótimo de oxigenação em fermentador nos parâmetros estabelecidos é de 2,0 lpm tanto para a produção de enzimática quanto para biomassa formada

Avaliação do envolvimento do receptor dectina-1 na atividade fungicida e na produção de metabólitos do oxigênio por neutrófilos e monócitos humanos desafiados com Paracoccidioides brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção da procedência de plantas psicoativas pelos isótopos estáveis do nitrogênio (15n) e oxigênio (18o)

This research examines the merits of hallucinogenic plants considered and/or psychoactive substances, such as Cannabis sativa and Ipomoea violacea, using the isotope rates of stable isotope of oxygen (18O) and nitrogen (15N), continuing projects already developed by the research group at the Center for Stable Isotope (CIE), which evaluated the isotopes carbon-13. This paper helps in creating a database that we intent to use in evaluation of each plant merit. Through the IRMS (Isotope-Ratio Mass Spectrometry) technique, it has shown that some of the 24 samples of marijuana (Cannabis sativa L.) evaluated were similar to those grown in the regions of Fairbanks and Tanacross Alaska, USA. In turn, the 50 samples of Ipomoea violacea, coming from Botucatu (SP) and Três Lagoas (MS), got their differences detected, in order to clearly identify the discrepancies between their growing regions. Thus, it was possible not only to track the geographical differences between marijuana samples collected from different regions, but also evaluate the isotopic variation of leaf, flower and seed of Ipomoea violacea. With this database, it was possible to determine the origin region of the drug and/or detect where the cultivation was carried out, aiding in the search of the traffic control agencies, such as the Federal Police in Brazil

Efeito do ácido linoleico e catalese sobre o desenvolvimento e criotolerãncia de embriões bovinos produzidos in vitro na ausência de soro e baixa tensão de oxigênio: Mônica Ferreira Accorsi. -

Pós-graduação em Ciência Animal - FMVA

Cinética do consumo de oxigênio e tempo limite na vvo2max: comparação entre homens e mulheres

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)