Effects of N-acetylcysteine on alcohol abstinence and alcohol-induced adverse effects in rats

Alcoholism is rampant in modern society and some antioxidant compound could perhaps be useful to reduce the damage done by alcohol consumption and abstinence. The present study was undertaken to investigate the association of N-acetylcysteine (NAC) intake, alcoholism, and alcohol abstinence on lipid profile, in vivo low-density lipoprotein (LDL) oxidation, oxidative stress, and antioxidant status in serum and liver of rats. Initially, male Wistar 30 rats were divided into two groups: (C, N = 6) given standard chow and water; (E, N = 24) receiving standard chow and aqueous ethanol solution in semi-voluntary research. After 30 days of ethanol exposure, (E) group was divided into four subgroups (N = 6/group): (E-E) continued drinking 30% ethanol solution; (E-NAC) drinking ethanol solution containing 2 g/L NAC (AB) changed ethanol solution to water; (AB-NAC) changed ethanol to aqueous solution 2 g/L NAC. After 15 days of the E-group division, E-E rats had higher serum alanine transaminase, lower body weight, and surface area, despite higher energy intake than C. E-E rats had also lower feed efficiency, dyslipidemia with enhanced triacyl glycerol, very low-density lipoprotein (VLDL), lipid hydroperoxide (LH) and in vivo oxidized-LDL (ox-LDL). AB, E-NAC, and AB-NAC rats ameliorated serum oxidative stress markers and normalized serum lipids. E-E rats had higher hepatic LH and lower reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio than C, indicating hepatic oxidative stress. AB and E-NAC rats normalized hepatic LH, GSSG, and the GSH/GSSG ratio, compared to E-E. AB-NAC rats had the lowest serum ox-LDL, hepatic LH levels, and the highest GSH reductase activity in hepatic tissue. In conclusion, the present study brought new insights into alcohol consumption, because ethanol exposure enhanced serum in vivo ox-LDL, as well as serum and hepatic oxidative stress. N-acetylcysteine offers promising therapeutic value to inhibit ethanol-induced adverse effects. Ethanol withdrawal had beneficial effects on serum lipids, but was more effective when coupled with NAC supplementation. Ethanol abstinence and NAC intake interact synergistically, improving serum lipids and hepatic antioxidant defenses. (c) 2009 Elsevier B.V. All rights reserved.

Seasonal metabolic depression, substrate utilisation and changes in scaling patterns during the first year cycle of tegu lizards (Tupinambis merianae)

The tegus increase in body mass after hatching until early autumn, when the energy intake becomes gradually reduced. Resting rates of oxygen consumption in winter drop to 20% of the values in the active season (Vo(2)=0.0636 ml g(-1) h(-1)) and are nearly temperature insensitive over the range of 17-25degreesC (Q(10)=1.55). During dormancy, plasma glucose levels are 60% lower than those in active animals, while total protein, total lipids and beta-hydroxybutyrate are elevated by 24%, 43% and 113%, respectively. In addition, a significant depletion of liver carbohydrate (50%) and of fat deposited in the visceral fat bodies (24%) and in the tail (25%) and a slight loss of skeletal muscle protein (14%) were measured halfway through the inactive period. Otherwise, glycogen content is increased 4-fold in the brain and 2.3-fold in the heart of dormant lizards, declining by the onset of arousal. During early arousal, the young tegus are still anorexic, although Vo(2) is significantly greater than winter rates. The fat deposits analysed are further reduced (62% and 45%, respectively) and there is a large decrease in tail muscle protein (50%) together with a significant increase in glycogen (2-3-fold) and an increase in plasma glucose (40%), which suggests a role for gluconeogenesis as a supplementary energy source in arousing animals. No change is detectable in citrate synthase activity, but beta-hydroxyacyl CoA dehydrogenase activities are strongly affected by season, reaching a Mold and 5-fold increase in the liver tissue of winter and arousing animals, respectively, and becoming reduced by half in skeletal muscle and heart of winter animals compared with late fall or spring active individuals. From hatching to late autumn, the increase of the fat body mass relatively to body mass is disproportionate (b=1.44), and the mass exponent changes significantly to close to 1.0 during the fasting period. The concomitant shift in the Vo(2) mass exponent in early autumn (b=0.75) to values significantly greater than 1.0 in late autumn and during winter dormancy indicates an allometric effect on the degree of metabolic depression related to the size of the fat stores and suggests greater energy conservation in the smaller young.

Desnutrição protéico-energética no paciente gastrectomizado

Racional - A gastrectomia traz conseqüências nutricionais inevitáveis mas atenuáveis, dependendo da dietoterapia pós-operatória. Embora observada, essa desnutrição protéico-energética é pouco dimensionada, provavelmente, pela falta de consenso metodológico. Objetivo - Avaliar o grau de desnutrição protéico-energética do paciente gastrectomizado, utilizando-se de indicadores isolados ou combinados. Pacientes e Métodos - Foram estudados 71 pacientes com gastrectomia parcial (n = 53) ou total (n =18) em pós-operatório de 6 a 24 meses e 24-60 meses. Os dados dietéticos, composição corporal e bioquímicos foram analisados de acordo com o tipo de gastrectomia e tempo pós-operatório. Resultados - A cirurgia foi conseqüência de complicações de úlcera péptica (68%) ou a câncer gástrico (32%). A perda de peso foi referida por 70% dos pacientes, sendo maior no grupo gastrectomia total (16 ± 5 kg) do que no grupo gastrectomia parcial (10 ± 6 kg). em geral, os pacientes apresentaram déficit antropométrico, albuminemia normal e baixa ingestão calórica, sugerindo deficiência energética crônica. A redução de hemoglobina, hematócrito e ferro ocorreu em maior intensidade e mais precocemente no grupo gastrectomia total. Assim, quando se associou hemoglobina aos indicadores albumina, linfócitos circunferência do braço e prega cutânea subescapular, a prevalência de desnutrição protéico-energética foi maior e em maior intensidade do que na ausência da hemoglobina. Conclusão - A gastrectomia resultou em desnutrição protéico-energética do tipo marasmática, acompanhada de anemia, mais intensa e precoce na gastrectomia total e gradativa na gastrectomia parcial, assemelhando-se à gastrectomia total no pós-operatório tardio.

Fatores associados à qualidade da dieta de adultos residentes na Região Metropolitana de São Paulo, Brasil, 2002

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo antropométrico e dietético de nadadores competitivos de áreas metropolitanas da região sudeste do Brasil

Com o objetivo de caracterizar os ábitos alimentares de nadadores competitivos de dois clubes das cidades do Rio de Janeiro e de São Paulo, foram estudados atletas, 30 homens e 37 mulheres pertencentes às equipes juvenil (15-17 anos) e seniors (18-25 anos). Concomitantemente à avaliação antropométrica, foi feito o inquérito alimentar mediante os métodos de registro alimentar, recordatório de 24 horas e freqüência de consumo alimentar. Os resultados mostraram semelhança antropométrica entre as duas faixas etárias para cada um dos sexos, semelhança dos alimentos prevalentemente ingeridos com aqueles referidos pela população da área metropolitana das duas cidades e semelhança entre os suplementos alimentares ingeridos pelos atletas dos dois clubes. A ingestão calórica observada foi quase o dobro da referida para a população daquelas localidades, mas equivalente à descrita para nadadores de outros países. Os lanches, entre refeições, contribuíram com 25-28% da ingestão calórica global e a maior contribuição energética alimentar foi dada pelo grupo de cereais. Apesar de contribuir com quase 50% do aporte calórico diário, a ingestão glicídica foi menor que a recomendada para atletas competitivos (55-60%). A ingestão protéica de 2,5-3,0 g/kg/dia superou em 100% o valor recomendado para atletas e ultrapassou os 15% na participação calórica diária. A ingestão lipídica foi considerada elevada, particularmente a de gordura saturada. O aporte de micronutrientes foi acima do referido para as populações locais em função da ingestão calórica elevada, mas mostrou-se relativamente inadequado em magnésio, ferro e vitaminas A e D. Conclui-se que o padrão alimentar dos nadadores estudados não difere, muito, do da população local, distinguindo-se pelo elevado e freqüente consumo de alimentos energéticos, maior no sexo masculino que no feminino.

Exigências de energia para mantença de bovinos zebuínos não-castrados em confinamento

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desempenho de bovinos jovens das raças Aberdeen Angus e Hereford, confinados e alimentados com dois níveis de energia

Estudou-se o desempenho de bovinos machos não-castrados das raças Aberdeen Angus (AA) e Hereford (HE) em confinamento, submetidos a dois níveis de energia, em esquema fatorial 2 x 2, sendo o menor nível com 3,07 e o maior com 3,18 Mcal/kg de energia digestível (12 e 32% de concentrado na dieta, respectivamente). Foram utilizados oito animais da raça AA e oito HE, com idade inicial de nove meses e peso médio inicial de 220,31 kg, que permaneceram confinados até que o peso da carcaça atingiu o mínimo de 190 kg (estimativa). Os animais da raça AA apresentaram maior consumo de MS, em % PV (2,27 vs 2,10%) e em g/kg0,75 (91,4 vs 86,4 g). Os animais que consumiram o maior nível de energia na dieta apresentaram maiores consumos de MS/dia (6,31 vs 5,71 kg), em PV (2,26 vs 2,11%) e em g/kg0,75 (92,28 vs 85,44 g), de energia digestível (ED), em Mcal/dia (20,58 vs 18,13 Mcal), e de PB, em kg/dia (0,845 vs 0,759 kg), além de maior ganho médio diário de peso (1,409 vs 1,250 kg). Os animais que consumiram o menor nível apresentaram maiores consumos de fibra em detergente neutro (FDN), em kg/dia (2,23 vs 2,07 kg), e de fibra em detergente ácido (FDA), em kg/dia (1,13 vs 1,01 kg). Os consumos de MS/dia, de FDN e de FDA, nos animais que consumiram o menor nível de energia, tiveram comportamento linear e, naqueles que receberam o maior nível, comportamento quadrático, frente aos períodos de confinamento. Para as características consumo de MS, em %PV e em g/kg0,75, nos tratamentos com menor nível de energia, o comportamento foi de forma cúbica e naqueles de maior nível, de forma quadrática. O consumo de ED apresentou, nos períodos, comportamento linear para o menor nível energético e cúbico para o maior nível.

Macro mineral requirements by grazing zebu bovines

The objective of this study was to determine requirements of calcium (Ca), phosphorus (P), magnesium (Mg), potassium (K) and sodium (Na) for grazing zebu bovines. The experiment area was composed of Brachiaria decumbens paddocks. Twenty-seven non-castrated animals, with initial live weight of 311.0 kg and at an average age of 14 months were used. Three animals were slaughtered, after adaptation period, so they were used as control for estimates of empty body weight and initial body composition of animals in the experiment. Out of the 24 remaining animals, four were sent to the maintenance group with restrict grazing time to limit energy intake close to the maintenance level. The other 20 animals were distributed in four treatments: mineral mixture, self-control intake and three-times-a-week-offer frequency (offered on Mondays, Wednesdays and Fridays) and daily. Concentrations of all studied macro elements in empty body and empty body gain decreased as live weight increased. The ratios obtained for g Ca/100 g of retained protein and g P/100 g of retained protein were 9.18 and 4.72, respectively. Total dietary requirement of calcium was lower than the one recommended by NRC (2000), but P requirement was very close to that.

Sojas integrais tostada pelo vapor e extrusada em rações de poedeiras

An experiment with 400 laying hens Hy Line with 26 weeks of age was conducted to compare the performance of laying hens fed during four cycles of 28 days with diets containing soybean meal (SM) plus soybean oil (SBM+oil), whole extruded soybean (ESB) and whole steam toasted soybean (TSB). A completely design randomized blocks was used, with 10 treatments and five replicates and eight laying hens in each experimental unit. The treatments consisted on the replacement of SBM per ESB and TSB at the levels 0, 25, 50, 75 and 100%; and as control the SBM with or without addition of oil. The results obtained showed that the hens were able to regulate the feed intake to maintain the energy intake only at lower energetic levels, however they tended to over intake energy with the increase of energy levels of the diets. The addition of oil or soybeans in the diets improved feed: gain ratio, however worsened the energy efficiency in relation to the diet without oil. The processing of soybean provided differences on the performance of laying hens and the ESB showed to be superior to TSB. The hens had higher use of the oil added to the soybean meal (SBM + oil) and ESB in relation to TSB. However, the values of AMEn obtained for the ESB were 12% higher, in average, to those determined for the SBM + oil and for the TSB.

Do all tannins have similar nutritional effects? A comparison of three Brazilian fodder legumes

Three tropical legumes, namely Leucaena leucocephala, Sesbania sesban and Cajanus cajan, were subjected to chemical analysis plus in vitro, in situ and in vivo evaluations. Three different assays were used to determine total tannins: adsorption to polyvinyl pyrrolidine (PVPP-tannins), radial diffusion (RD-tannins) and protein precipitation capacity (BSA-tannins). Total phenols, total tannins and condensed tannins were highest for Sesbania. RD-tannins were correlated with total phenols (r(2) = 0.93), PVPP-tannins (r(2) = 0.92) and condensed tannins (r(2) = 0.99). The protein precipitation capacity of Sesbania, Leucaena and Cajanus were 25.9, 6.13 and 4.05 mu g BSA/g DM, respectively.Gas production at 24h was negatively correlated with total phenols (r(2) = 0.99), PVPP-tannins (r(2) = 0.99) and condensed tannins (r(2) = 0.91). The RD-, PVPP-tannins and the response to polyethylene glycol (PEG) in the gas production assay appeared to be useful as a first screen for tannins.In situ degradability did not reflect any adverse effects of tannins. However, in vivo experiments showed that the apparent DM digestibility of Sesbania and Leucaena was lower than the basal diet. The apparent protein digestibility was lower for all legumes compared to the basal diet. Most treatments caused a negative nitrogen balance. The problems associated with browse feeding were not only related to tannin contents, other factors such as inherently poor digestibility and low energy intake may also have lead to the poor animal performance on these diets. We propose, given the limitations of current tannin assays, that it is not possible to predict beneficial or harmful nutritional effects from total tannin concentrations per se. (C) 2004 Elsevier B.V. All rights reserved.

Effect of tannin levels in sorghum silage and concentrate supplementation on apparent digestibility and methane emission in beef cattle

This study evaluated the effect of diets containing sorghum silages with higher (HT) and lower-tannin (LT) concentrations supplemented with concentrate or urea on intake, digestibility, ruminal digestibility, methane emission and rumen parameters in beef cattle. Four treatments were distributed according to a 2 x 2 factorial arrangement in a duplicate 4 x 4 Latin square: LT sorghum silage + urea, LT sorghum silage + concentrate, HT sorghum silage + urea, and HT sorghum silage + concentrate. Total digestibility of the organic matter was higher when concentrate was included in the diet (0.749 and 0.753 in the LT and HT treatments, respectively). It was observed lower ruminal apparent digested matter of neutral detergent fiber in HT diets. There was no effect of tannin levels on digestibility and methane emission. The supplementation with concentrate in the LT diet decreased gas losses as a function of gross energy intake in comparison to the supplementation of the diet with urea. These results suggest the potential of concentrate supplementation to minimize energy loss as methane emission by ruminants and increase the efficiency of energy utilization. (c) 2006 Elsevier B.V. All rights reserved.

Desempenho Produtivo de Animais de Quatro Raças Zebuínas, Abatidos em Três Estádios de Maturidade. 1. Ganho de Peso e de Carcaça e Eficiência de Ganho

This research was conducted to evaluate the productive performance (live weight gain and carcass weight gain and gain efficiency) of animals from four zebu breed slaughtered in three maturity stages. Thirty-six bulls from Gyr, Guzera, Mocho Tabapua and Nellore with twenty-four months of average age and average initial live weight of 357.6, 362.0, 368.6 and 376.4 kg, respectively, were used. The bulls, from each breed, were randomly assigned to individual pens and were full-fed a diet containing 50% concentrate (DM basis) and distributed to three category (1, 2 and 3) of slaughter weights. The bulls from category 1, 2 and 3 were slaughtered when reached the individual live weight of 405, 450 and 500 kg, respectively. At slaughter the empty body weight was determined. The Nellore breed bulls showed greater average empty body weight gain and carcass gain in relation to the other breeds, which did not differ among themselves. There were no differences among breeds regarding to feed efficiency (empty body weight gain efficiency and carcass gain efficiency per unit of metabolizable energy intake). The slaughter weight did not influence the empty body weight gain and carcass gain, but animals slaughtered at higher slaughter weights showed lower gain efficiency and spent more time in feedlot.

Anthropometrical parameters and markers of obesity in rats

The present study was undertaken to determine anthropometrical parameters in male adult Wistar rats. We tested the hypothesis that the anthropometrical index may identify obesity and may predict its adverse effects on lipid profile and oxidative stress in rats. Two experimental protocols were performed. in the first experiment, 50 male Wistar rats, 21 days old and fed a control chow were studied up to 150 days of age. in the second experiment, male Wistar rats, 60 days old, were divided into three groups (n = 8): control (C) given free access to a control chow; (S) receiving the control chow and drinking 30% sucrose ad libitum and (HQ fed a high-carbohydrate diet ad libitum. The first experiment showed that food consumption, energy intake and body weight increased with increasing age, while specific rate of body mass gain was significantly decreased. There were no significant differences in body length and thoracic circumference of rats from 60 days of age. The abdominal circumference (AC) and body mass index (BMI) significantly increased with enhancing age in rats up to 90 days of age and remained constant thereafter. In the second experiment, after 30 days of dietary treatment, the final body weight, body mass gain, carcass fat and BMI were higher in S and HC rats than in C. There were no significant alterations in body length and carcass protein among the groups. Triacylglycerol (TG), total cholesterol (CT), low-density lipoprotein cholesterol (LDL-C) and lipid hydroperoxide (LH) were higher in S and HC rats than in C. High-density lipoprotein cholesterol (HDL-C) decreased in HC rats and total antioxidant substances (TAS) decreased in S and HC rats. There were positive correlations between BMI with carcass fat, BMI with LH and BMI and serum TG concentration. In conclusion, the BMI for male adult Wistar rats ranged between 0.45 and 0.68 g/cm(2). Obesity may be easily estimated from the BMI in rats. Alterations in BMI were associated with dyslipidemic profile and oxidative stress in serum of rats and BMI may predict these adverse consequences of the obesity in rats.

Efeito dos níveis de energia da ração sobre o desempenho e teor de gordura na carcaça de frangos de corte

It was studied the effect of energy levels in the diet, obtained from vegetal oil inclusion, on the performance, diet cost per kilogram of broiler and fat content of the carcass, There were used 4,800 broiler chicks, 2,400 males and 2,400 females, and they were distributed in six treatment (energy levels in kcal ME/kg of diet) in three different phases: start diet from one to 20 days of age: A - 2930, B - 2980, C - 3030, D - 3080, E - 3130, F - 3180, growth diet from 21 to 40 days: A - 2980, B - 3030, C - 3050, D - 3130, E - 3150, F - 3230, finishing diet from 41 to 45 days of age: A - 3030, B - 3080, C - 3130,, D - 3180, E - 3230, F - 3280. The protein content was 22, 20 and 18% in each phase, respectively, There were evaluated the feed intake, energy intake, protein intake, weight gain, feed conversion mortality, diet cost per kg/bw. Sixty broilers were slaughtered, five males and five females per treatment, randomly chosen to determine the carcass composition considering the dry matter content, lipids content and protein content, total fat in carcass and fat per kilogram of broiler. The data were submitted to variance analysis and the means were compared using SNK. The broilers fed with medium and low levels of dietary energy showed the highest weight gain. At energy levels of the treatment D in the three phases, the lowest cost per kilogram of broiler was obtained. The females presented the highest fat content per kilogram of broiler produced.

Long-term nutrition education reduces several risk factors for type 2 diabetes mellitus in Brazilians with impaired glucose tolerance

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)