Detecção de herpesvírus bovino 5 (BoHV-5) em bovinos do Sudeste Brasileiro

This paper reports the detection of bovine herpesvirus type 5 (BoHV-5) by a specific nested PCR assay. Samples were collected from the central nervous system (CNS) of cattle from Minas Gerais and São Paulo States, Brazil. All animals died presenting neurological symptoms. Nineteen frozen CNS samples analyzed had been previously tested by fluorescence antibody test for rabies virus and showed negative results. Three paraffin-embedded brain tissue samples were examined by histopatology and the observed alterations suggested nonsuppurative meningoencephalitis. BoHV-5 was detected in five (22.7%) among 22 tested samples. The occurrence of BoHV-5 infection is reported in the Southeast region of Brazil, indicating that epidemiological studies should be carried out.

Estudo citogenético em equinos para detecção de portadores de monossomia do cromossomo X

A cytogenetic study of 22 mares with fertility has showed that four of them had 63,X/64,XX mosaicism. The X-chromosome has presented the expected interstitial heterochromatic C - banding located in the long arm, besides of the usual centromeric band. The great variation of clinic signs observed in mares with mosaicism, could be due to the type of zygote or the time the mosaicism occured.

Utilização do metabolismo protéico ( 15N-glicina) na detecção precoce de agravamento da atividade da doença em pacientes portadores de retocolite ulcerativa inespecífica

Disease activity was assessed in 10 (five males and five females) ulcerative colitis patients through the following parameters: clinical, laboratory, sigmoidoscopic and histological. Protein metabolism was also assessed with 15N-glycine and urinary ammonia as end product. Only one patient had exacerbation of the disease two months after the study started. This patient presented in the beginning of the study protein synthesis and breakdown of 4.51 and 3.47 g protein/kg/day, respectively, values higher than all other patients, showing an hypermetabolic state, suggesting an increase of the disease activity. However, this increase was not detected by others indicators and indexes utilised. These data allow to suggest the hypothesis that protein metabolism predicts precociously the exacerbation of disease activity in ulcerative colitis patients.

Aplicação do Índice Relaçáo Declividade-Extensão - RDE Na Bacia do Rio do Peixe (SP) para Detecção de Deformações Neotectô nicas

This paper presents the identification of tectonic deformation through the application of the stream-gradient index (slope x length) method in the Rio do Peixe hydrographic basin, located in western State of Sã o Paulo. The main principle used is that river valleys are the best tools for this type of analysis because of their fast adjustment to even the most gentle crustal deformations. This type of analysis for structural studies is extremely useful in the western region of the State of São Paulo, where deep chemical weathering results in a thick regolith and scarce outcrops. The stream length index is the ratio of the altimetric amplitude of each drainage and the natural logarithm of its length. The value obtained is plotted on maps at the median point of each drainage, allowing the drawings of lines with the same values (isodefs). Three anomalies (A, B and C) were identified along the Rio do Peixe valley representing uplift sectors indicated by the formation and distribution of Upper Quaternary deposits in terraces and modern fluvial plains and alluvial fans. In correlation with known tectonic data for this region it is possible to attribute the A and B anomalies to the Ribeirão Preto and Presidente Prudente crustal sutures, respectively. The smaller C anomaly needs further field investigation, but it could be associated with the Três Lagoas crustal suture. It must be emphasised that these sutures are inherited from the Precambrian Basement which had a strong influence on the Phanerozoic evolution in the whole sedimentary and volcanic stratigraphic pile of the Paraná Basin and on the dominant structural lineaments directions developed therein .The A, Band C anomalies are suggestive of renewed uplift as a result of neotectonic action in modern times.

Classificação automática de padrões projetados por um sistema de luz estruturada

One of the main problems in Computer Vision and Close Range Digital Photogrammetry is 3D reconstruction. 3D reconstruction with structured light is one of the existing techniques and which still has several problems, one of them the identification or classification of the projected targets. Approaching this problem is the goal of this paper. An area based method called template matching was used for target classification. This method performs detection of area similarity by correlation, which measures the similarity between the reference and search windows, using a suitable correlation function. In this paper the modified cross covariance function was used, which presented the best results. A strategy was developed for adaptative resampling of the patterns, which solved the problem of deformation of the targets due to object surface inclination. Experiments with simulated and real data were performed in order to assess the efficiency of the proposed methodology for target detection. The results showed that the proposed classification strategy works properly, identifying 98% of targets in plane surfaces and 93% in oblique surfaces.

Detecção de pistas de aeroporto em imagens orbitais utilizando morfologia matemática

The Brazilian Cartography presents great deficiency in cartographic products updating. This form, Remote Sensins techniques together Digital Processing Images - DPI, are contributing to improve this problem. The Mathematical Morphology theory was used in this work. The principal function was the pruning operator. With its were extracted the interest features that can be used in cartographic process updating. The obtained results are positives and showed the use potential of mathematical morphology theory in cartography, mainly in updating.

Extração semi-automática de rodovias em um par de imagens aéreas digitais utilizando geometria epipolar e programação dinâmica

The purpose of this paper is to introduce a methodology for semi-automatic road extraction from aerial digital image pairs by using dynamic programming and epipolar geometry. The method uses both images from where each road feature pair is extracted. The operator identifies the corresponding road featuresand s/he selects sparse seed points along them. After all road pairs have been extracted, epipolar geometry is applied to determine the automatic point-to-point correspondence between each correspondent feature. Finally, each correspondent road pair is georeferenced by photogrammetric intersection. Experiments were made with rural aerial images. The results led to the conclusion that the methodology is robust and efficient, even in the presence of shadows of trees and buildings or other irregularities.

Orientação semi-automática de imagens CBERS usando rodovias como controle de campo

In this paper is proposed a methodology for semiautomatic CBERS image orientation using roads as ground control. It is based on an iterative strategy involving three steps. In the first step, an operator identifies on the image the ground control roads and supplies along them a few seed points, which could be sparsely and coarsely distributed. These seed points are used by the dynamic programming algorithm for extracting the ground control roads from the image. In the second step, it is established the correspondences between points describing the ground control roads and the corresponding ones extracted from the image. In the last step, the corresponding points are used to orient the CBERS image by using the DLT (Direct Linear Transformation). The two last steps are iterated until the convergence of the orientation process is verified. Experimental results showed that the proposed methodology was efficient with several test images. In all cases the orientation process converged. Moreover, the estimated orientation parameters allowed the registration of check roads with pixel accuracy or better.

Detecção de prováveis deformações neotectônicas no Vale do Rio do Peixe, Região Ocidental Paulista, mediante aplicaçao de Índices RDE (Relac̈ão Declividade-Extensão) em segmentos de drenagem

Running water is one of the most important of all the physical processes which fashion the landscape, allowing gravity to operate along the valley floors. Besides this, the streams show a fast adjustment to the crustal deformations, even to the most gentle ones. This geologic behavior turns them a potential tool for neotectonic studies, specially the analysis of morphotnetric parameters associated with hydraulic gradient and discharge, this second factor being directly proportional to the extension of the streams. Both elements, gradient and stream length, can be combined in the SL index. The purpose of this paper is to show the RDE index application in the neotectonics analysis of the Rio do Peixe hydrographic basin and to compare the obtained values with the geologic basement incised by the streams. This basement encompasses Cretaceous sedimentary rocks of post-Serra Oeral Formation magmatism (Caiuá and Bauru groups) and Quaternary deposits that include chiefly recent alluvial plains and some Pleistocene terrace deposits. In the final part of this paper, an attempt is made in order to correlate the RDE results and the neotectonic framework admitted to this portion of the São Paulo State territory, as well as with field geologic, seismologic and paleoseismologic known elements. The results indicate the presence of two groups of anomalies: The first set corresponds to the Marília-Exaporã Plateau border, and the second one, located in the central portion of the hydrographic basin, is correlated to the Presidente Prudente seimogenic zone.

Detecção de Leptospira pomona em sêmen bovino por eletroforese capilar fluorescente

This study was performed in order to evaluate the detection limit of PCR with fluorescent capillary electrophoresis for Leptospira pomona diagnosis in bovine semen. Negative bovine semen samples were artificially contaminated with Leptospira pomona (10 0 to 10 7 bacteria/ ml) and DNA was extracted by phenol/chloroform protocol. DNA fragments visualization was done by three electrophoresis methods: under UV light in 2 % agarose gel, silver staining 8% polyacrylamide gel and fluorescent capillary electrophoresis. The detection limit of capillary electrophoresis for Leptospira pomona was 10 2bacteria/ml. Under UV light, in 2 % agarose gel, the detection limit was of 10 4 bacteria/ ml while for silver stained 8 % polyacrylamide gel it was 10 2 bacteria/ ml. PCR with fluorescent capillary electrophoresis is an efficient and rapid diagnostic test for DNA detection of Leptospira in bovine semen and this can be an important tool for herd and semen sanitary control in artificial insemination centers.

Detecção de bordas em imagens digitais através de um processo de difusão anisotrópica não linear

The edges detection model by a non-linear anisotropic diffusion, consists in a mathematical model of smoothing based in Partial Differential Equation (PDE), alternative to the conventional low-pass filters. The smoothing model consists in a selective process, where homogeneous areas of the image are smoothed intensely in agreement with the temporal evolution applied to the model. The level of smoothing is related with the amount of undesired information contained in the image, i.e., the model is directly related with the optimal level of smoothing, eliminating the undesired information and keeping selectively the interest features for Cartography area. The model is primordial for cartographic applications, its function is to realize the image preprocessing without losing edges and other important details on the image, mainly airports tracks and paved roads. Experiments carried out with digital images showed that the methodology allows to obtain the features, e.g. airports tracks, with efficiency.

Detecção da produção de slime por estafilococos coagulase-negativa isolados de cateter venoso central

Slime production is an important virulence factor of coagulase-negative Staphylococcus spp., allowing them to attach to smooth surfaces of biomaterials, and it has been associated with infections of implanted medical devices. In the present study the production of slime capsules in 27 strains of coagulase-negative Staphylococcus was investigated by culture in Congo Red agar (77.7% positivity), spectrophotometric or microplate method (81.4% positivity) and scanning electron microscopy (88.9% positivity). The resistance of coagulase-negative strains of Staphylococcus to various antimicrobial agents was also determined by agar disk diffusion. The proportion of strains resistant to penicillin G, oxacillin, erythromycin, clindamycin and gentamicin among the slime-producing staphylococci was 88.9%, 70.4%, 81.5%, 66.7% and 59.2%, respectively; all of the coagulase-negative staphylococci were susceptible to vancomycin. The strains isolated from central venous catheters were identified by a conventional method and the API Staph system. The 27 coagulase-negative Staphylococcus strains were identified as: S. saprophyticus (3.7%), S. xylosus (7.4%), S. haemolyticus (14.8%), S. epidermidis (37.0%), S. warneri (14.8%), S. lugdunensis (7.4%), S. hominis (7.4%), S. schleiferi (3.7%) and S. chromogenes (3.7%). It can be concluded that in the most of the coagulase-negative Staphylococcus species there was an association between slime production, the nosocomial origin of the strains and reduced sensitivity to the antibiotics, suggesting a pathogenic potential in the hospital environment.

Utilização do método de aglutina ção direta e da reação de imunofluorescência indireta na detecção de anticorpos para Toxoplasma gondii em cavalos naturalmente infectados

Infection by Toxoplasma gondii in equines is usually not apparent, it being characterized by presence of antibody titers and tissue cysts. This study was aimed at verifying the presence of anti-Toxoplasma antibodies in equine serum by modified agglutination test (MAT) and reaction to indirect immunofluorescent antibody test (IFAT). 1984 samples of serum were examined, by MAT, using whole formalin fixed tachyzoites of T.gondii as antigen. The samples reacting in the MAT test, and 150 other negative samples, chosen at random, were also tested by IFAT, utilizing anti-equine IgG. The association among the test results was verified by the McNemar test. 138 samples were positive in the MAT test, with 60 (46.38%) presenting reaction at a dilution of 1:64; 52 (37.7%) at 1:256; 19 (13.8%) at 1:1024; five (3.6%) at 1:4096; and two (1.45%) at 1:16384. Of 132 positive MAT samples, 14 were negative in the IFAT test, but the statistical analysis indicated general agreement in results of the tests. The results obtained showed agreement among the tests utilized, and the possibility of participation of equines in the transmission of toxoplasmosis to carnivorous animals, and also to humans.

Aplicabilidade do TTC para a detecção de bacteriúria

Laboratory tests to detect urinary infection at a lawer cost than uroculture are necessary. Triphenyl tetrazolium chloride was evaluated in parallel with uroculture in 342 urine samples, for the detection of significant bacteriuria. The results showed that the test has high sensitivity (91.3%) but low specificity (64.3%), and negative predictive value 99.0%. Although the test cannot replace uroculture as a diagnostic method, it can be recommended for preliminary screening, and could eliminate the need for uroculture of negative samples.