Integrated study by NMR and X-ray Crystallography on the analysis of the molecular interactions in heme-binding proteins

Disserta����o para obten����o do Grau de Doutor em Bioqu��mica, Especialidade Bioqu��mica Estrutural

Molecular determinants of ligand specificity in carbohydrate-binding modules: an NMR and X-ray crystallography integrated study

Disserta����o para obten����o do Grau de Doutor em Bioqu��mica ��� Ramo Bioqu��mica Estrutural

Estudo de implementa����o da NP EN ISO 22000:2005 na sala de desmancha de carnes frescas da empresa ���X��� Cash & Carry

Disserta����o para obten����o do Grau de Mestre em Tecnologia e Seguran��a Alimentar

Electron impact ionization cross sections and analysis of X-ray spectra

A thesis submitted for the degree of Ph. D. in Physics

Scanning electron microscopy and X-ray spectroscopy applied to mycelial phase of sporothrix schenckii

Scanning electron microscopy applied to the mycelial phase of Sporothrix schenckii shows a matted mycelium with conidia of a regular pattern. X-Ray microanalysis applied in energy dispersive spectroscopy and also in wavelength dispersive spectroscopy reveals the presence of several elements of Mendeleef's classification.

Encefalite Cr��nica a Enterov��rus num Doente com Agamaglobulin��mia Cong��nita Ligada ao Cromossoma X

Os autores apresentam um caso cl��nico de encefalite cr��nica a enterov��rus num rapaz de 9 anos com agamaglobulin��mia cong��nita ligada ao cromossoma X (doen��a de Bruton). Apesar da terap��utica intraventricular com doses elevadas de gamaglobulina, registou-se uma progress��o da doen��a com deteriora����o neurol��gica maci��a e morte. Discutem-se os aspectos diagn��sticos e terap��uticos desta situa����o.

Variant Rett Syndrome in a Girl with a Pericentric X-Chromosome Inversion Leading to Epigenetic Changes and Overexpression of the MECP2 Gene

Rett syndrome is a neurodevelopmental disorder caused by mutations in the MECP2 gene. We investigated the genetic basis of disease in a female patient with a Rett-like clinical. Karyotype analysis revealed a pericentric inversion in the X chromosome -46,X,inv(X)(p22.1q28), with breakpoints in the cytobands where the MECP2 and CDKL5 genes are located. FISH analysis revealed that the MECP2 gene is not dislocated by the inversion. However, and in spite of a balanced pattern of X inactivation, this patient displayed hypomethylation and an overexpression of the MECP2 gene at the mRNA level in the lymphocytes (mean fold change: 2.55��0.38) in comparison to a group of control individuals; the expression of the CDKL5 gene was similar to that of controls (mean fold change: 0.98��0.10). No gains or losses were detected in the breakpoint regions encompassing known or suspected transcription regulatory elements. We propose that the de-regulation of MECP2 expression in this patient may be due to alterations in long-range genomic interactions caused by the inversion and hypothesize that this type of epigenetic de-regulation of the MECP2 may be present in other RTT-like patients.

Epidemiologia da doen��a de Chagas no Cear�� X - h��bitos alimentares dos vetores

Foram pesquisados 7 munic��pios do Estado, sendo o maior n��mero de amostras colhido em Russas, Morada Nova, Quixad�� e Iracema. Foram feitas provas de precipitina com anti-soros de gato, c��o, ave, cabra, roedor, marsupial e homem. De 1.205 provas realizadas, 392 apresentaram prova positiva (32,5%). Apresentaram 79,3% uma s�� fonte alimentar, 19,4% duas, 1% tr��s e 3% quatro. Os resultados foram os seguintes:

Bowel X-ray alterations in acute human schistosomiasis

A radiological study of the small intestine of 17 untreated patients in the acute phase ofschistosomiasis was performed. Twelve patients (70% of total) had alterations: nine had clear-cut thickening of the duodenal and jejunal folds, one flocculation, one fragmentation and one thickening of mucosae, flocculation and fragmentation of the barium column. There was no correlation of the gastrointestinal symptomatology (vomiting, diarrhoea, dysentery, hepatomegaly) neither with the parasitological load nor with the x-ray alterations.

Estudo necrosc��pico de um caso da forma cr��nica da doen��a de Chagas com eletrocardiograma e raio-X de t��rax normais: refer��ncia especial ao sistema excito-condutor do cora����o

Descrevem-se as altera����es anatomopatol��gicas observadas na necropsia de chag��sico cr��nico, provavelmente, portador da forma indeterminada da doen��a. O cora����o mostrou les��es dos tr��s folhetos e do sistema excito-condutor, traduzidas especialmente por pequenos focos inflamat��rios. Havia discreta fibrose do mioc��rdio contr��til, n��dulo ��trio-ventricular e origem do ramo esquerdo do feixe de His. Tais altera����es, embora de leve intensidade, assemelham-se, qualitativamente, ��s relatadas nas demais formas cr��nicas da doen��a de Chagas e representam les��es ativas, com potencial evolutivo. No es��fago, observou-se entre outros fatos, despopula����o neuronal acentuada.

Estudo da efici��ncia energ��tica do edif��cio X da FCT/UNL

Disserta����o para obten����o do Grau de Mestre em Engenharia Mec��nica

Elabora����o de um diagrama TAC para um a��o de alta tenacidade API X-70

Disserta����o para obten����o do Grau de Mestre em Engenharia de Materiais

Ecos da XXII Reuni��o Anual de Pesquisa Aplicada em Doen��a de Chagas e X Reuni��o Anual de Pesquisa Aplicada em Leishmanioses

A doen��a de Chagas e as leishmanioses s��o importantes condi����es que ainda afetam milh��es de pessoas, com relevantes implica����es m��dicas, sociais e econ��micas. As reuni��es anuais sobre doen��a de Chagas e leishmanioses s��o marcantes f��runs de discuss��o sobre novos achados acerca destas doen��as, bem como de planejamento de estrat��gias de controle, que envolvem grande n��mero de conceituados e de jovens pesquisadores. Assim, essas Reuni��es devem continuar com todo vigor representando a luta contra tais doen��as.

Modelling of a wastewater treatment plant using GPS-X

Dissertation to obtain the degree of Master in Chemical and Biochemical Engineering

Experimental lagochilascariosis in X-chromosome-linked immunodeficient mice

Lagochilascaris minor is the etiological agent of lagochilascariosis, a disease that affects the neck region and causes exudative abscesses, with eggs, adult parasites and L3/L4 larvae in the purulent exudates. Mice are now considered to be intermediate hosts for the parasite. To determine the pattern of infection in B1 cell-deficient mice, experimental lagochilascariosis was studied in BALB/c and X-chromosome-linked immunodeficient (xid) mice. BALB.xid-infected mice showed lower numbers of larvae. Third-stage larvae, fourth-stage larvae and adult parasites were found in both strains. BALB/c mice produced IgM, IgG, IgA and IgE against the crude extract and secreted/excreted antigens of the parasite. On the other hand, BALB.xid mice did not produce IgM and produced lower levels of IgG and IgA, and similar quantities of IgE.