Structural characterization of interaction of K11 and K63 mixed-linkage polyubiquitin chains with the tUIMs of epsin1

Ubiquitylation or covalent attachment of ubiquitin (Ub) to a variety of substrate proteins in cells is a versatile post-translational modification involved in the regulation of numerous cellular processes. The distinct messages that polyubiquitylation encodes are attributed to the multitude of conformations possible through attachment of ubiquitin monomers within a polyubiquitin chain via a specific lysine residue. Thus the hypothesis is that linkage defines polyubiquitin conformation which in turn determines specific recognition by cellular receptors. Ubiquitylation of membrane surface receptor proteins plays a very important role in regulating receptor-mediated endocytosis as well as endosomal sorting for lysosomal degradation. Epsin1 is an endocytic adaptor protein with three tandem UIMs (Ubiquitin Interacting Motifs) which are responsible for the highly specific interaction between epsin and ubiquitylated receptors. Epsin1 is also an oncogenic protein and its expression is upregulated in some types of cancer. Recently it has been shown that novel K11 and K63 mixed-linkage polyubiquitin chains serve as internalization signal for MHC I (Major Histocompatibility Complex I) molecule through their association with the tUIMs of epsin1. However the molecular mode of action and structural details of the interaction between polyubiquitin chains on receptors and tUIMs of epsin1 is yet to be determined. This information is crucial for the development of anticancer therapeutics targeting epsin1. The molecular basis for the linkage-specific recognition of K11 and K63 mixed-linkage polyubiquitin chains by the tandem UIMs of the endocytic adaptor protein epsin1 is investigated using a combination of NMR methods.

The fountain of sacred song : a collection of new church music, for choirs, congregations, singing schools and conventions /

Publisher's advertisements: back cover.

The village harmony, or, New-England repository of sacred musick /

For 3-4 voices.

Twelve sacred choral works.

Psalm 2, op.78, no. 1.--Psalm 43, op.78, no.2.--Psalm 22, op. 78, no. 3.--Psalm 100.--3 Motetten, op.69.--6 Sprüche, op.79.--2 Geistliche Chöre, op.115.--Trauergesang, op.116.--Ehre sei Gott in der Höhe.--Heilig.--Kyrie eleison.--Zum Abendsegen.

Giffe's new male quartet book : a choice collection of new secular quartets for male voices /

Includes index.

Hawthorn and lavender. [music] : a song-cycle for women's voices with piano accompaniment /

Poems printed as text: p. 3-5.

The chorus and choir : a superior collection of standard choruses, part-songs and anthems suitable for colleges, normals, choirs, choral societies, musical conventions, singing schools, etc., etc. /

Includes index.

The Treasury of song for the home circle: the richest, best-loved gems, sacred and secular ... to which are appended brief biographies of eminent composers, performers and singers ...

Added t.p., colored.

Campath-1G in vivo confers a low incidence of graft-versus-host disease associated with a high incidence of mixed chimaerism after bone marrow transplantation for severe aplastic anaemia using HLA-identical sibling donors

We have evaluated the effect of in vivo Campath-1G on engraftment and GVHD in 23 patients with severe aplastic anaemia transplanted from HLA-identical sibling donors. In 14 patients Campath 1g was given pre-transplant for up to 9 days in an attempt to overcome graft rejection (group 1). In nine patients Campath-1G was given pre-transplant, but also continued post-transplant until day +5 to reduce GVHD (group 2). There were three patients with late graft failure in group I following initial neutrophil engraftment, and four cases of grade II+ GVHD. In group II, two patients had early graft failure (no take), and there were no cases of acute GVHD out of seven evaluable patients. One patient in group I developed chronic GVHD of the liver, and two patients (one in each group) had transient localised chronic GVHD. PCR of short tandem repeats was used to evaluate chimaeric status in 13 patients. Of 11 patients with initial neutrophil engraftment, only one had 100% donor haemopoiesis at all times. The remaining patients had either transient mixed chimaerism or persistence of recipient (< 20%) cells. We conclude that in vivo Campath-1G is associated with a high incidence of mixed chimaerism which tips the balance away from GVHD but towards graft rejection.

Normannenzug.

Mode of access: Internet.

Landerkennung (Landkjending)

In portfolio.

Vor der Klosterpforte; aus Björnson's "Arnljot Gelline." Für Solostimme, Frauenchor und Orchester. Op. 20.

In portfolio.

Eingangschor aus der Kantate: Lasset uns ablegen die Werke der Finsternis : komponiert 1749 /

For mixed voices (SATB) and piano.

Festgesgang an die Künstler, for men's chorus and brass, op.68;

Mode of access: Internet.