蹼铃蟾蛋白酶抑制剂及其制备方法和在制药中的应用

本发明属于生物医学领域。大蹼铃蟾蛋白酶抑制剂，从两栖类动物大蹼铃蟾皮肤中分离得到的一种单链丝氨酸蛋白酶抑制剂，其在SDS－聚丙烯酰胺凝胶电泳上的表观分子量还原条件下为67KDa，非还原条件下为45KDa，等电点4.8，糖含量17—19％，无酶活性，大蹼铃蟾蛋白酶抑制剂的N—端31个氨基酸序列结构是：DSETHIRFLGEVYKKVDTIDFRGLVLITRAQ。其制备方法是取大蹼铃蟾皮肤，在生理盐水中匀浆后，离心去除沉淀，收集上清液冷冻干燥后，经离子交换，凝胶过滤纯化后得到。具有强烈的丝氨酸蛋白酶抑制活性和抗肿瘤活性，作为制备肿瘤治疗药物和制备治疗胃炎、胰腺炎及其他炎症药物的应用。

云南几个地方鸡种的mtDNA限制性片段多态性研究

云南几个地方鸡种的ｍｔＤＮＡ限制性片段多态性研究邹平张廷饮（云南省畜牧兽医科学研究所昆明６５０２２４）王文陈永久张亚平（中国科学院昆明动物研究所细胞与分子进化开放实验室线粒体ＤＮＡ（ｍｔＤＮＡ）具有进化速度快、严格单性母系遗传和易于提取分析而成为研...

中国太阳鸟科鸟类新纪录——褐喉直嘴太阳鸟

2009年12月14—15日,武汉观鸟爱好者廖晓东(网名:僵鱼)分别在云南省勐腊县中国科学院西双版纳热带植物园和景洪市云南省热带作物科学研究所内拍摄到取食花蜜的小型鸟类。经鉴定,所摄两张照片中的鸟,均属褐喉直嘴太阳鸟

Product life trade-offs: what if products fail early?

Increasing product life allows the embodied emissions in products to be spread across a longer period but can mean that opportunities to improve use-phase efficiency are foregone. In this paper, a model that evaluates this trade-off is presented and used to estimate the optimal product life for a range of metal-intensive products. Two strategies that have potential to save emissions are explored: (1) adding extra embodied emissions to make products more sturdy, increasing product life, and (2) increasing frequency of use, causing early product failure to take advantage of improvements in use-phase efficiency. These strategies are evaluated for two specific case studies (long-life washing machines and more frequent use of vehicles through car clubs) and for a range of embodied and use-phase intensive products under different use-phase improvement rate assumptions. Particular emphasis is placed on the fact that products often fail neither at their design life nor at their optimal life. Policy recommendations are then made regarding the targeting of these strategies according to product characteristics and the timing of typical product failure relative to optimal product life.

Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue

An essential for respiration and viability (ERV1) homologue, 88R, was cloned and characterized from Rana grylio virus (RGV). Database searches found its homologues in all sequenced iridoviruses, and sequence alignment revealed a highly conserved motif shared by all ERV1 family proteins: Cys-X-X-Cys. RT-PCR and western blot analysis revealed that 88R begins to transcribe and translate at 6 h postinfection (p.i.) and remains detectable at 48 h p.i. during RGV infection course. Furthermore, using drug inhibition analysis by a de novo protein synthesis inhibitor and a viral DNA replication inhibitor, RGV 88R was classified as a late (L) viral gene during the in vitro infection. 88R-EGFP fusion protein was observed in both the cytoplasm and nucleus of pEGFP-N3-88R transfected EPC cells. Although result of immunofluorescence is similar, 88R protein was not detected in viromatrix. Moreover, function of RGV 88R on virus replication were evaluated by RNAi assay. Nevertheless, effect of knockdown of RGV 88R expression on virus replication was not detected in cultured fish cell lines. Collectively, current data indicate that RGV 88R was a late gene of iridovirus encoding protein that distributed both the cytoplasm and nucleus.

Revision of the genus Niwaella in China (Pisces, Cobitidae), with description of two new species

Loaches of the genus Niwaella, family Cobitidae, are distributed only in East Asia. At present only in Japan and South Korea have fishes of the genus Niwaella been found. Herein we revise the genus Niwaella in China. Son and He ( 2001) transferred the species Cobitis laterimaculata to the genus Niwaella, but their specimens were not N. laterimaculata, but a new species, N. longibarba sp. n., collected from Cao'ejiang River, Huangzezhen, Chengxian County, Zhejiang Province. The new species is distinguished from N. laterimaculata by its colour pattern of a row of slightly large, and long, scattered dark brown vertical bars on the dorsolateral surface, two or three striations on the caudal fin, and long barbels and undeveloped mental lobes. In this paper we also describe another new species, N. xinjiangensis sp. n., collected from Xinjiang River, Guangfeng County, Jiangxi Province, May 1990, with diagnostic colour pattern of 17 - 20 large and long, dark brown vertical bars on the dorsolateral surface, a dark stripe or rounded black spots along the lateral midline and some blotches below the lateral midline; it is a large-sized species, with shorter barbels, and longer caudal peduncle. Thus five species of the genus Niwaella are known, three are endemic to eastern China and two are endemic to either Japan or South Korea.

Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)

The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareouirus, family Reouiridae, were sequenced. The respective segments 1, 2, and 3 were 3949, 3877, and 3702 nucleotides long. Conserved moths 5' (GUUAUUU) and 3' (UUCAUC) were found at the ends of each segment. Each segment contains a single ORF and the negative strand does not permit identification of consistent ORFs. Sequence analysis revealed that VP2 is the viral polymerase, while VPI might represent the viral guanyly/methyl transferase (involved in the capping process of RNA transcripts) and VP3 the NTPase/helicase (involved in the transcription and capping of viral RNAs), The highest amino acid identities (26-41%) were found with orthoreovirus proteins. Further genomic characterization should provide insight about the genetic relationships between GCRV, aquareoviruses, and orthoreoviruses, It should also permit to precise the taxonomic status of these different viruses. (C) 2000 Academic Press.

Fabrication and characterization of GaN-based LEDs grown on nanopatterned sapphire substrates

Sapphire substrates were nanopatterned by dry (inductively coupled plasma, ICP) etching to improve the performance of GaN-based light-emitting diodes (LEDs). GaN-based LEDs on nanopatterned sapphire substrates (NPSS) were fabricated by metal organic chemical vapor deposition (MOCVD). The characteristics of LEDs fabricated on NPSS prepared by dry etching were studied. The light output power and wall-plug efficiency of the LEDs fabricated on NPSS were greater than those of the conventional LEDs fabricated on common planar sapphire substrates when the injection currents were the same. The LEDs on NPSS and common planar sapphire substrates have similar I-V characteristics.

Fabrication of triplexers based on flattop SOI AWG

A triplexer is fabricated based on SOI arrayed waveguide gratings (AWGs). Three wavelengths of the triplexer operate at different diffraction orders of an arrayed waveguide grating. The signals of 1490 nm and 1550 nm, which are input from central input waveguide of an AWG, are demultiplexed and the signal of 1310 nm, which is input from central output waveguide of an AWG, is uploaded. The tested results show that the downloaded and uploaded signals have flat-top response. The insertion loss is 9 dB on chip, the nonadjacent crosstalk is less than -30 dB for 1490 nm and 1301 nm, and is less than -25 dB for 1550 nm, the 3 dB bandwidth equates that of the input light source.

基于量化权限的门限访问控制方案

研究了在引入量化权限观点后从访问控制角度实现秘密保护的问题.元权限是从哲学上"质"和"量"的角度认识传统意义上的权限所探究出的新概念,较以往访问控制中认识和使用权限而言,它全面而深入地反映了权限这一概念的本质.进一步结合门限思想和基于角色的访问控制机制所提出的基于量化权限的门限访问控制方案,从访问控制的角度研究了秘密保护问题.在秘密保护方面,基于量化权限的门限访问控制方案具有一些独特的优点,比如分发给参与者的秘密分片和要保护的秘密无知识上的联系、可以反映出参与者信任度的差异以及运算量低.