Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima

Amphibian skin is a rich resource of antimicrobial peptides, like maximins and maximin Hs from frog Bombina maxima. Novel cDNA clones encoding a precursor protein, which comprises a novel maximin peptide (maximin 9) and reported maximin H3, were isolated from two constructed skin cDNA libraries of B. maxima. The predicted primary structure of maximin 9 is GIGRKFLGGVKTTFRCGVKDFASKHLY-NH2. A surprising substitution is at position 16, with a free cysteine in maximin 9 rather than usual conserved glycine in other reported maximins. Maximin 9, the homodimer form and its Cys(16) to Gly(16) mutant were synthesized and their antimicrobial activities were evaluated. Unlike previously reported maximin 3, the tested bacterial and fungal strains were resistant to maximin 9, its homodimer and the Cys(16) to Gly(16) mutant (with MICs > 100 mu M). On the other hand, interestingly, while eight clinical Mollicutes strains were generally resistant to maximin 9 homodimer and its Cys(16) to Gly(16) mutant, most of them are sensitive to maximin 9 at a peptide concentration of 30 mu M, especially in the presence of dithiothreitol. These results indicate that the presence of a reactive Cys residue in maximin 9 is important for its antimycoplasma activity. The diversity of antimicrobial peptide cDNA structures encountered in B. maxima skin cDNA libraries and the antimicrobial specificity differences of the peptides may reflect well the species' adaptation to the unique microbial environments. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Evolutionary history of the mtDNA 9-bp deletion in Chinese populations and its relevance to the peopling of east and southeast Asia

In total, 1218 Chinese from twelve ethnic groups and nine Han geographic groups were screened for the mtDNA 9-bp deletion motif. The frequency of the 9-bp deletion in all samples was 14.7% but ranged from 0% to 32% in the various ethnic groups. Three individuals had a triplication of the 9-bp segment. Phylogenetic and demographic analyses of the mtDNA hypervariable segment 1 (HVS1) sequences suggest that the 9-bp deletion occurred more than once in China. The majority of the Chinese deletion:haplotypes (about 90%) have a common origin as a mutational event following an initial expansion of modem humans in eastern Asia. Other deletion haplotypes and the three haplotypes with a 9-bp triplication may have arisen independently in the Chinese, presumably by replication error. HVS1 haplotype analysis suggests two possible migration routes of the 9-bp deletion in east and southeast Asia. Both migrations originated in China with one route leading to the Pacific Islands via Taiwan, the other to southeast Asia and possibly the Nicobar Islands. Along both routes of peopling, a decrease in HVS1 diversity of the mtDNA haplotypes is observed. The "Polynesian motif (16217T/C, 16247A/G, and 16261C/T)" and the 16140T/C, 16266C/A, or C/G polymorphisms appear specific to each migration route.

Frequency of the mtDNA 9-bp deletion in Chinese ethnic groups

The 9-bp deletion in the COII/tRNA(Lys) intergenic region (region V) of human mitochondrial DNA was screened in 1521 Chinese from 16 ethnic groups and 9 Hen geographic groups. The highest frequency was found in populations of Miao (32.4%) and Bouyei (30.8

The effect of increase in the number of fishing trips on the economic efficiency of 9.82 m and 11 m fishing trawlers along Kerala coast

Cost-benefit analysis of a 9.82 m and 11 m fishing trawlers based on the number of fishing trips is presented. The number of fishing trips per year determines the profit and loss of the trawler. With the increase in the number of fishing trips, the profit also increase for both the sizes of trawlers. The minimum quantity of prawn and fish to be landed for 0-20% profit for varying number of fishing trips are worked out. The break-even for 9.82 and 11m trawlers was observed to be 185 and 210 fishing trips respectively during 1980-81.

点柄乳牛肝菌子实体中抗HIV-1活性成分

目的 研究点柄乳牛肝菌的化学成分,并对分离鉴定的化合物进行抗HIV-1的活性研究.方法 将野外采集的点柄乳牛肝菌子实体用溶剂提取,采用硅胶柱色谱进行分离,通过波谱技术(NMR,MS,IR等)对结构进行鉴定.结果 分离鉴定了9个化合物,分别为:酒渣碱(Ⅰ)、5a,8a-过氧麦角甾-6,22-二烯-3β-醇(Ⅱ)、麦角甾-5,7,22-三烯-3β-醇(Ⅲ)、麦角甾-5,7,22-三烯-3β-O-β-D-吡喃葡萄糖苷(Ⅳ)、尿嘧啶(Ⅴ))、硫代乙酸酐(Ⅵ)、硬脂酸(Ⅶ)、3-吡啶甲酸(Ⅷ)和D-阿洛糖醇(Ⅸ).结论 化合物Ⅰ为首次从高等真菌中分离.经生物活性测试,该化合物具有抗HIV-1活性,对C8166细胞的毒性较小,CC50为87.86 μg/mL,对HIV-1诱导C8166细胞形成合胞体抑制的EC50为7.27 μg/mL,治疗指数(TI值)为12.09.

Group Size and Composition of Black．and．White Snub．nosedMonkey(rhinopithecus)Estimated by Faecesof Sleeping Sites at Baima Snow Mountain

介绍了应用过夜地粪便来估计白马雪山黑白仰鼻猴群大小和组成的一种方法。该物种以单雄多雌单 元和全雄组的形式在树上过夜。粪粒根据其大小可分为3种类型：成年雄性的(最大)、成年雌性的(中等大小)和 未成年个体的(最小)。2000一2001年，搜集了滇西北白马雪山国家级自然保护区北部南任村(99。04 7E，28。34 7N) 附近黑白仰鼻猴群每个季节2个过夜地的粪粒。根据2001年11月猴群通过开阔地的数据来确定猴群组成。每个 季节，由于单雄多雌单元的成年个体数与其粪粒数正相关，所以二者回归直线的斜率可以看作是每个个体每晚 的平均排便量。由于该物种的栖息地主要为高山峡谷，而且能见度较低，因此，利用过夜地粪便比以前通过猴群 活动痕迹来估计猴群大小和组成相对准确、可靠。从估计成年雌性个体数的角度看，利用粪粒来估计种群大约有 9．4％的偏差。导致偏差的可能原因有杂草和灌丛对粪粒准确计数的影响、个体排粪率的差异以及成年雄性最小 粪粒与成年雌性最大粪粒的混淆等。该方法适应于栖息地和主要食物与本文研究种群相似的其他种群。

中国9种嗜子宫线虫系统发育的初步研究

为了探讨鱼类寄生嗜子宫线虫的系统发育关系,测定了8种嗜子宫线虫的ITS rDNA(核糖体转录内间隔区核 糖核酸)序列和9种嗜子宫线虫的18S rDNA(小亚基核糖体核糖核酸)部分序列,并构建了18S rDNA序列的系统发 育树。在比较和分析ITS rDNA和18S rDNA两种分子标记对嗜子宫科线虫系统发育适用性的基础上,分析了嗜子 宫线虫的系统发育关系。结果表明:中国嗜子宫线虫是单系起源;黄颡鱼似嗜子宫线虫、赣州似嗜子宫线虫和棍头 嗜子宫线虫亲缘关系非常接近,可能是较晚形成的种;似嗜子宫线虫属可能应该被

Magnetocaloric effect and magnetic properties of Tb0.9 Sn0.1 MnO3

The magnetocaloric effect in magnetic materials is of great interest nowadays. In this article we present an investigation about the magnetic properties near the magnetic transition in a polycrystalline sample of a manganite Tb0.9 Sn0.1 MnO3. Particularly, we are interested in describing the nature of the magnetic interactions and the magnetocaloric effect in this compound. The temperature dependence of the magnetization was measured to determine the characteristics of the magnetic transition and the magnetic entropy change was calculated from magnetization curves at different temperatures. The magnetic solid is paramagnetic at high temperatures. We observe a dominant antiferromagnetic interaction below Tn =38 K for low applied magnetic fields; the presence of Sn doping in this compound decreases the Ńel temperature of the pure TbMnO3 system. A drastic increase in the magnetization as a function of temperature near the magnetic transition suggests a strong magnetocaloric effect. We found a large magnetic entropy change Δ SM (T) of about -4 J/kg K at H=3 T. We believe that the magnetic entropy change is associated with the magnetic transition and we interpret it as due to the coupling between the magnetic field and the spin ordering. This relatively large value and broad temperature interval (about 35 K) of the magnetocaloric effect make the present compound a promising candidate for magnetic refrigerators at low temperatures. © 2007 American Institute of Physics.

保安湖麦穗鱼种群生物学V.生产力

在 1 995年 1 0月至 1 996年 1 1月 ,依据有关种群参数估算了保安湖 1 # 试验围拦中麦穗鱼的生产力。月平均瞬时生长率的变化与年龄和季节有关 ,随着年龄增加生长率降低 ,在生活史的早期阶段生长最快 ,生长率一般在春、夏季较高。在研究期间 ,种群平均生物量为1 8 2 7kg·hm- 2 ,周年生产力为 36 53kg·hm- 2 ·a- 1 ,P/B系数为 2 0。 1 996世代对总生产力的贡献为 67 9%。种群月平均生物量及生产力均表现出了较明显的季节变化 ,生物量在秋季(9月 )