Influência do extrato pirolenhoso na calda de pulverização sobre o teor foliar de nutrientes em limoeiro 'Cravo'

Com o objetivo de avaliar o efeito da presença do extrato pirolenhoso (EP) na calda de pulverização sobre o teor foliar de nutrientes de limoeiro 'Cravo' (Citrus limonia Osbeck), foi desenvolvido um experimento com seis tratamentos e quatro repetições, em blocos ao acaso, em ambiente protegido. Os tratamentos constituíram da pulverização das soluções: T0 = água; T1 = solução de micronutrientes sem EP; T2 = solução de micronutrientes + EP (1cm³ dm-3); T3 = solução de micronutrientes + EP (2 cm³ dm-3); T4 = solução de micronutrientes + EP (5cm³ dm-3); T5 = solução de micronutrientes + EP (10 cm³ dm-3). A solução de micronutrientes foi preparada com sulfatos de Cu, Fe, Mn, Zn (250 mg dm-3 do elemento) e ácido bórico (42,5 mg dm-3 de B). As plantas foram cultivadas em tubetes cônicos de 0,280 dm³, com substrato sem a adição de micronutrientes na formulação. As soluções foram pulverizadas uma única vez, aos 140 dias após o plantio (DAP), momento em que as plantas apresentavam aproximadamente 20 cm de altura. Ao final do experimento (160 DAP), quantificaram-se a massa seca e os teores de macro e micronutrientes da parte aérea e sistema radicular. A presença do extrato pirolenhoso na solução de micronutrientes não interferiu na concentração foliar de B, Fe e Zn em mudas de limoeiro 'Cravo'. Entretanto, na concentração de 10 cm³ dm-3, aumentou a concentração foliar de Cu e Mn. Observou-se também que as plantas pulverizadas com soluções contendo EP (1 a 10 cm³ dm-3) + micronutrientes apresentaram menor teor de Fe e maior teor de Ca no sistema radicular.

Propofol ou sevofluorano sobre variáveis hemodinâmicas em cães submetidos à administração subaracnóidea de iohexol

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphologic changes in the vesical transition epithelium of alcoholic rats

Few studies are available about the effect of alcohol on the epithelium of the urinary bladder. In the present investigation we studied the ultrastructure of the vesical transition epithelium of normal rats and of rats submitted to experimental chronic alcoholism. Adult rats were submitted to experimental chronic alcoholism by the ingestion of sugar cane liquor. The vesical epithelium was examined after 60, 120, 180 and 240 days of alcohol treatment by transmission electron microscopy. Surface cells presented nuclear and cytoplasmic changes and marked cellular desquamation. There was an increase in multivesicular bodies and lysosomes suggesting cell degeneration. Mast cell infiltration was observed, possibly related to increased epithelial sensitivity. Intercellular spaces were frequently observed. The transition epithelium of the urinary bladder was found to be sensitive to the action of alcohol, as demonstrated by the changes in the components of the blood-urine barrier, the greater sensitivity to inflammation, the increase in cell desquamation and the greater recycling of the apical membrane and of the fusiform vesicles of surface cells observed in alcoholic rats.

Cinética da remoção dos extrativos da madeira de Eucalyptus grandis durante polpação Kraft

Kinetics of the removal of Eucalyptus grandis wood extractives during Kraft pulping. The objective of this work was to study the kinetics of the removal of Eucalyptus grandis wood extractives during Kraft pulping. The pulping was done in steel tubular reactors using wood saw under the following conditions: active alkali = 14%, sulfidity = 25%; liquor-to-wood ratio = 10 L kg(-1) of dry wood; and temperatures of 130, 150, 160 and 180 C during 30, 60, 90, 120, 150 and 180 minutes. After pulping, analyses were conducted of the yield and extractives in residue (pulp and reject). Total extractives (ethyl alcohol-toluene (1:2), ethyl alcohol and hot water, respectively) and soluble extractives in acetone were analyzed. The results showed that most extractives are degraded in the first 30 minutes of the pulping process. It was observed that the removal occurs in two different stages: the first is rapid, where the majority of the extractives are removed, and the last is slow, where few extractives are removed.

Economic and technical feasibility study of a combined-cycle cogeneration system associated with cellulose plants

In this paper, is presented an economical and technical feasibility study of a combined cycle cogeneration system proposed to be used in a pulp plant located in Brazil, where around 95% of country's pulp production is done by the use of Kraft Process. This process allows the use of black liquor and other by-products as fuel. This study is based upon actual data from a pulp plant with a daily production of 1000 tons., that generates part of the energy demanded by the process in a conventional cogeneration system with condensing steam turbine and two extractions. The addition of a gas turbine was studied to compare electricity production level and its related costs between original system and the new one, considering that the former can use industrial by-products and firewood as fuel, when required. Several parameters related to electric generation systems operation and production costs were studied. The use of natural gas in the combined cycle, in comparison with the use of firewood in the conventional system was studied. The advantages of natural gas fuel are highlighted. The surplus availability and the electricity generation costs are presented as a function of pulp and black liquor production.

Cumulative effects of vinasse on the characteristics of red-yellow latosols under cerrado vegetation

Vinasse, a liquor effluent from the alcohol and sugar making industry, was applied annually for twelve years to medium-textured red-yellow latosols under cerrado vegetation sensu stricto, to study the environmental impacts on the biotic and abiotic factors. Four plots were established of which two acted as control and the other two received annual doses of vinasse. The studies were begun in 1980 when the first annual dose of 20 L m-2 year-1 was added to the soil without removing the top scrub layer. Theses doses were added to the soil until 1983, but in 1984 the doses were increased to 50 L m-2 year-1 and used until 1991. Soil samples were taken at a depth of 15 cm every three months from 1987 to 1991. Twenty seven environmental variables in the vinasse-treated and untreated plots were studied. These factors consisted of different enzymatic activities, a number of filamentous fungi, bacteria, actinomycetes and other micro-organisms, nutrients and some micro-climatic factors. The results obtained were statistically analyzed using the Tukey test, Pearson correlation and variance test methods with replicates and three factors. Matrices were determined using the correlation coefficient method and were compared with those of earlier published studies in the same area. The comparison of the results helped characterize changes in the environmental factors studied and in the correlation between them, after using annual cumulative doses of vinasse. Positive effects were observed only for the first six years of this application but vinasse had negative effects after the seventh year. It is concluded that medium-textured red-yellow latosols cannot be treated with vinasse for proloned periods.

Bacillus thuringiensis development from 1971 to 1996: Cases of a research group in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ultrastructural alterations of choroid plexuses of lateral ventricles of rats (rattus norvegicus) submitted to experimental chronic alcoholism

Adult male rats (Wistar lineage) were alcoholized with sugar cane liquor diluted at 30° GL during 300 days and sacrificed every 60 days in 5 stages. Samples of choroid plexuses of lateral ventricles were collected and examined at transmission electronic microscope to detect possible ultrastructural alterations and to raise possible pathological correlations. Gradual changes were observed in these animals during all the experiment: dilatation and enlargement of cisternae of Golgi complex, dilatation of RER, presence of digestive vacuoles and a large amount of pinocytic vesicles as well as vesicles with electronlucent content throughout cytoplasm, as well as an enlargement of intercellular space between basolateral interdigitation of the cells and of the connective tissue. The changes observed in the epithelium and connective tissue of choroid plexuses specially in 240 and 300 days of treatment are presumably due to a disturbance in hydroelectrolitic homeostasis, contributing to several morpho-functional disturbs of central nervous system. No changes were observed in the control group animals.

Bioethanol - Brazil, 30 years of Proálcool

Brazil, which has always been in the forefront of sugarcane production, also occupies a prominent position as the first country to produce and use biofuel in its automobile fleet. This fact is a consequence of the introduction of a program which has already turned 30 years, the Próalcool (National Alcohol Program). The oil crisis in the seventies encouraged the government to develop an alternative way to replace gasoline. Bioethanol was then born as fuel obtained from fermentation of sugarcane juice, molasses or both. In the eighties, 85% of the cars ran exclusively on alcohol. Ethanol production in that decade exceeded sugarcane production by the mills. The installed units reached in that period the capacity to produce 18 billion liters of bioethanol per season, a volume equivalent to 100 million barrels of gasoline. The fermentation process, which so far had been restricted to manufacturing sugarcane liquor (aguardente) or ethanol as a byproduct of sugarcane, takes over the spotlight in the entrepreneurial scene. As a result, processes comprising engineering concepts came up and most of the biological phenomena involved in fermentation were understood. The knowledge gathered and the units installed have granted Brazil the hold of production technology and use of a clean fuel.

L(+) Lactic acid production by new Lactobacillus rhamnosus B 103

L(+) Lactic acid fermentation was studied by Lactobacillus rhamnosus sp. under the effects of pH control and a lowcost nutritional medium (sugarcane juice and corn steep liquor-CSL). Central composite design (CCD) was employed to determine maximum lactic acid production at optimum values for process variables and a satisfactory fit model was realized. Statistical analysis of the results showed that the linear and quadratic terms of two variables (sugarcane juice and pH) had significant effects. The interactions between the three variables were found to contribute to the response at a significant level. A second-order polynomial regression model estimated that the maximum lactic acid production of 86.36 g/L was obtained when the optimum values of sucrose, CSL and pH were 112.65 g/L, 29.88 g/L and 6.2, respectively. Verification of the optimization showed that L(+) lactic acid production was of 85.06 g/L. Under these conditions, Y P/S and Q P values of 0.85 g/g and 1.77 g/Lh, respectively, were obtained after 48 h fermentation, with a maximal productivity of 2.2 g/L h at 30 h of process. © 2010 de Lima CJB, et al.

Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti

The bacteria Bacillus thuringiensis var. israelensis (Bti) generates certain toxins with pesticide action, which can be used on the control of transmissible diseases by culicides, specially Aedes aegypti, the dengue vector. This biopesticide has been produced by submerged fermentation and, in Brazil, this production has been made by very little research centers and, more recently, by a unique small enterprise. For the implementation of a viable vectors control program through biopesticides, some studies about culture media are essential in order to join efficiency and low costs. In this way, agroindustrial wastes or by-products have been used as a nutrient source for the culture media production. In this study, corn steep liquor, a corn industrial processing by-product and tryptose, both with/without sugar addition, were compared as culture media. Cellular growth was evaluated by optical density at 620 nm, spore production by total viable cell count and LC50 by bioassays against 4th instar larvae. Among the four examined substrates, the medium composed by glucose plus corn steep liquor presented the best spore production and bioassay results.

Fontes de carbono de baixo custo para a produção de xilanase termoestável por aspergillus niger

A strain of the flamentous fungus Aspergillus niger was isolated and shown to possess extracellular xylanolytic activity. These enzymes have biotechnological potential and can be employed in various industries. This fungus produced its highest xylanase activity in a medium made up of 0.1% CaCO3, 0.5% NaCl, 0.1% NH4Cl, 0.5% corn steep liquor and 1% carbon source, at pH 8.0. A low-cost hemicellulose residue (powdered corncob) proved to be an excellent inducer of the A. niger xylanolytic complex. Filtration of the crude culture medium with suspended kaolin was ideal for to clarify the extract and led to partial purifcation of the xylanolytic activity. The apparent molecular mass of the xylanase was about 32.3 kDa. Maximum enzyme activity occurred at pH 5.0 and 55-60oC. Apparent Km was 10.41 ± 0.282 mg/mL and Vmax was 3.32 ± 0.053 U/mg protein, with birchwood xylan as the substrate. Activation energy was 4.55 kcal/mol and half-life of the crude enzyme at 60oC was 30 minutes. Addition of 2% glucose to the culture medium supplemented with xylan repressed xylanase production, but in the presence of xylose the enzyme production was not affected.

Interação patógeno-hospedeiro no patossistema capim limão-ferrugem das folhas e avaliação de ingredientes ativos naturais no controle da doença

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo comprarativo da biossorção dos metais terras-raras ('ND POT. +3', 'LA POT. +3', 'CE POT. +3') pela biomassa melanizada e pigmento, na forma livre e imobilizada, obtidos do fungo Aspergillus nidulans

Pós-graduação em Biotecnologia - IQ

Uso do extrato pirolenhoso: Efeito no solo e nas plantas de tomate

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)