401 resultados para ovine


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Zootecnia - FCAV

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Streptococcosis is one of the major causes of mortality in tilapia's creation in Brazil, inducing great economic losses. As soon, the study objectived to determinate the frequency of isolation and identification the Streptococcus agalactiae in organs different of Oreochromis niloticus naturally infected, derived from eight fish farms in the northern region of the state of Parana, that presented clinical signs characteristics of streptococcal disease. However, blood samples and fragments (kidney, liver, spleen, heart and brain) were collected. These all samples were plated on solid medium of brain and heart infusion (BHI) added 5% ovine blood and incubated at 29 degrees C for 7 days in aerophilic conditions. Behind, the bacterial growth and from the macro and microscopic features, colonies compatibles with Streptococcus sp. gender, were selected. The species were identified by PCR reaction and confirmed by sequencing of 16S rDNA gene. The results exhibited that in tilapia of Nile infected with S. agalactiae the isolation is more common in brain, kidney and liver in descending order.

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Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 x 10(5) P strain oocysts; GII, 3 rams, 1.0 x 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (IIF) were performed. Presence of the parasite in the semen was investigated by PCR and bioassay techniques. The rams presented clinical alterations (hyperthermia and apathy) related to toxoplasmosis in both groups infected with Toxoplasma gondii. All the inoculated rams responded to antigenic stimulus, producing antibodies against T. gondii from postinoculation day 5 onwards. In ovine groups I and II, the greatest titers observed were 1 : 4096 and 1 : 8192, respectively. In semen samples collected from these two groups, the presence of T. gondii was detected by bioassay and PCR. This coccidian was isolated (bioassay and PCR) in tissue pools (testicles, epididymis, seminal vesicle, and prostrate) from two rams infected presenting oocysts and in one presenting tachyzoites.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Mastitis is an acute or a chronic inflammation of the udder, represented for physical, chemical and bacteriological milk alterations. It is notable that ovine milk represents high economic importance, and it can be used in the production of cheeses and other derivates, contributing for the farmer’s recipe enlargement. However, the mastitis in animals can determine the reduction of milk quantity and quality in infected animals. This may determine lambs’ weight loss and increase animal’s mortality. By the other hand, considering Public Health issues, mastitis can cause milk nutrients losses and promote the microorganisms’ multiplication that may lead to diarrheas and to other zoonosis in humans. This study aimed to evaluate 159 sheep mastitis status in 20 small farms located in Bauru (SP) region. Of the 309 colected samples, in 97 (31.4%) were isolated microorganisms of the genus Staphylococcus sp., which them 27.5% being coagulase negative Staphylococcus and 3.9% coagulase positive Staphylococcus. Of 177 isolates, 97 (54.8%) were represented by the genus Staphylococcus sp., which them 57 (58.8%) from pure culture and 40 (41.2%) in association. Most agents isolated were sensitive to antibiotics used in the test. Thus, we could verify the isolation of infectious microorganisms such as coagulase positive and negative Staphylococcus and, as well in dairy cattle, preventive measures to obtain hygienic milk are extremely important in order to get a final product with quality and safe in food and microbiological point of view.