Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis

Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).

Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil

INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.

Les fleurs animees /par J.-J. Grandville ; introductions par Alph. Karr ; texte par Taxile Delord.

vol. 2

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 1 1991

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 2 1992

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 3 1993

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 4 1994

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 5 1995

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 6 1996

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 7 1997

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 8 1998

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 9 1999

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 10 2000

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 11 2001

Novon a journal of botanical nomenclature from the Missouri Botanical Garden.

v. 12 2002