Nanopartíulas de magnetita com oxacilina obtida por moagem de alta energia para aplicações biomédicas

The drug targeting has been the subject of extensive studies in order to develop site-specific treatments that minimize side effects and become more effective anticancer therapy. Despite considerable interest in this class, drugs like antibiotics also have limitations, and have been neglected. Using new pharmaceutical technologies, the use of magnetic vectors appear as promising candidate for drug delivery systems in several studies. Small magnetic particles bound to the drug of interest can be modulated according to the orientation of a magnet outside the body, locating and holding in a specific site. In this work, we propose the use of High Energy Milling (HEM) for synthesis of a magnetic vector with characteristics suitable for biomedical applications by intravenous administration, and for the formation of an oxacillin-carrier complex to obtain a system for treating infections caused by Staphylococcus aureus. The results of the variation of milling time showed that the size and structural properties of the formed material change with increasing milling time, and in 60 hours we found the sample closest to the ideal conditions of the material. The vector-drug system was studied in terms of structural stability and antimicrobial activity after the milling process, which revealed the integrity of the oxacillin molecule and its bactericidal action on cultures of Staphylococcus aureus ATCC

Variabilidade espacial de atributos do solo para adoção do sistema de agricultura de precisão na cultura de cana-de-açúcar

O objetivo do presente trabalho foi avaliar a variabilidade espacial de atributos de Latossolos sob cultivo de cana-de-açúcar na região de Jaboticabal (SP). Foram feitas amostragens do solo a intervalos regulares de 50 m, em uma área de 90 ha, nas profundidades de 0,00-0,20 e 0,60-0,80 m para determinação de pH, CTC, V % e teores de matéria orgânica, P, K+, Ca2+, Mg2+, H + Al e argila. Os dados foram submetidos às análises: estatística descritiva, geoestatística e interpolação por krigagem. Os alcances de dependência espacial para os atributos químicos do solo e teores de argila na camada de 0,60-0,80 m de profundidade foram menores, quando comparados àqueles referentes à camada de 0,00-0,20 m. Estes resultados demonstraram maior descontinuidade na distribuição espacial dos atributos do solo na camada de 0,60-0,80 m de profundidade dos Latossolos, indicando que essa classe de solos não apresentou homogeneidade de seus atributos como conceituadamente a ela foi atribuída. O manejo no solo alterou a dependência espacial dos atributos do solo na camada superficial de forma a diminuir a variabilidade espacial dos atributos químicos do solo em relação à camada mais profunda. A investigação da variabilidade espacial de atributos químicos e do teor de argila da camada superficial e subsuperficial dos solos proporcionou condições para a definição de zonas homogêneas de manejo, o que permite a adoção do sistema de agricultura de precisão.

Uso da krigagem indicatriz na avaliação de indicadores de fertilidade do solo

Este trabalho teve como objetivo identificar zonas diferenciadas de manejo por meio de indicadores de fertilidade em Latossolos cultivados com cana-de-açúcar, utilizando-se a técnica de krigagem indicatriz, com vistas em buscar um aprimoramento na utilização de técnicas de agricultura de precisão. O estudo foi realizado em uma área de 90 ha pertencente a uma área maior de 1.900 ha, localizada em Jaboticabal, Estado de São Paulo, Brasil (21 ° 15 ' S e 48 ° 18 ' W). Na área experimental, foi estabelecida uma malha de amostragem regular de 50 m, com 420 pontos. em cada ponto, coletou-se uma amostra de solo, na qual foram determinados os teores de matéria orgânica, P, K disponíveis e o valor de V. Os dados obtidos para estas características do solo, assim como as combinações entre elas, foram codificados em valores indicadores 0 ou 1, conforme as variáveis apresentassem valores acima ou abaixo do valor de corte estabelecido, escolhido para cada variável, respectivamente. Os resultados propiciaram a confecção de mapas de probabilidade de cada variável (individual e combinada), o que possibilitou identificar regiões com diferentes níveis de fertilidade do solo na área experimental.

Influência da variabilidade espacial de atributos químicos de um latossolo na aplicação de insumos para cultura de cana-de-acúcar

As técnicas de aplicação localizada e em taxas variadas de insumos agrícolas com auxílio da geoestatística têm sido umas das áreas de concentração de esforços recentes em pesquisas e desenvolvimento ligado à agricultura de precisão. Portanto, o trabalho teve como objetivo avaliar a necessidade de aplicação de calcário e fósforo com base no comportamento espacial de atributos químicos do solo. Foram feitas amostragens do solo a intervalos regulares de 50 m, em uma área de 42 ha, na profundidade de 0,0-0,2 m para determinação do pH, Ca, Mg, P, V% e cálculo da necessidade de calagem e superfosfato triplo. Os dados foram submetidos às análises da estatística descritiva, geoestatística e interpolação por krigagem. O uso da técnica de geoestatística possibilitou uma precisa descrição dos atributos do solo o que permitiu a definição de zonas de manejo, indicando os locais de ocorrência de déficit e excesso. A variabilidade espacial dos atributos químicos do solo, possibilita a recomendação de doses de calcário e fósforo com taxas variadas, proporcionando economia e maior eficiência na aplicação.

Nitric oxide release using natural rubber latex as matrix

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genetic structure of populations of Rhizoctonia solani anastomosis group-1 IA from soybean in Brazil

The Basidiomycete fungus Rhizoctonia solani anastomosis group (AG)-1 IA is a major pathogen of soybean in Brazil, where the average yield losses have reached 30 to 60% in some states in Northern Brazil. No information is currently available concerning levels of genetic diversity and population structure for this pathogen in Brazil. A total of 232 isolates of R. solani AG1 IA were collected from five soybean fields in the most important soybean production areas in central-western, northern, and northeastern Brazil. These isolates were genotyped using 10 microsatellite loci. Most of the multilocus genotypes (MLGTs) were site-specific, with few MLGTs shared among populations. Significant population subdivision was evident. High levels of admixture were observed for populations from Mato Grosso and Tocantins. After removing admixed genotypes, three out of five field populations (Maranhao, Mato Grosso, and Tocantins), were in Hardy-Weinberg (HW) equilibrium, consistent with sexual recombination. HW and gametic disequilibrium were found for the remaining soybean-infecting populations. The findings of low genotypic diversity, departures from HW equilibrium, gametic disequilibrium, and high degree of population subdivision in these R. solani AG-1 IA populations from Brazil are consistent with predominantly asexual reproduction, short-distance dispersal of vegetative propagules (mycelium or sclerotia), and limited long-distance dispersal, possibly via contaminated seed. None of the soybean-infecting populations showed a reduction in population size (bottleneck effect). We detected asymmetric historical migration among the soybean-infecting populations, which could explain the observed levels of subdivision.

Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Integridade biótica de um córrego na bacia do Alto Rio Paraná avaliada por meio da comunidade de peixes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Variabilidade espacial de atributos do solo antes e após calagem e fosfatagem em doses variadas na cultura de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Variabilidade espacial de atributos químicos e de produtividade na cultura do café

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Transforming open mining pits into fish farms: Moving towards sustainability

The legacy of mining activities has typically been land 'returned to wildlife', or, at some sites, degraded to such an extent that it is unsuitable for any alternate use. Progress towards sustainability is made when value is added in terms of the ecological, social and economic well-being of the community. In keeping with the principles of sustainable development, the innovative use of flooded open pits and tailings impoundments as commercial, recreational or ornamental fish farms should be considered in some locations, as it could make a significant contribution to the social equity, economic vitality and environmental integrity of mining communities. This article highlights the growing significance of aquaculture and explores the benefits and barriers to transforming flooded pits and impoundments into aquaculture operations. Among other benefits, aquaculture may provide a much-needed source of revenue, employment and, in some cases, food to communities impacted by mine closure. Further, aquaculture in a controlled closed environment may be more acceptable to critics of fish farming who are concerned about fish escapes and viral transmissions to wild populations. Despite the potential benefits, aquaculture in flooded pits and impoundments is not without its complications - it requires a site-specific design approach that must consider issues ranging from metals uptake by fish, to the long-term viability of the aquatic system as fish habitat, to the overall contribution of aquaculture to sustainability. © 2004 United Nations. Published by Blackwell Publishing.

Variabilidade espacial do estado nutricional do cafeeiro canephora visando o manejo localizado

Mapping the plant nutritional condition allows viewing different regions in a cropping area, providing the producers with different criteria to use foliar and soil fertilization. The aim of this study was to evaluate the spatial variability of the nutritional condition of canephora coffee (Coffea canephora Pierre ex Froehner) regarding the site specific management of foliar and soil fertilization. In a one hectare area 60 georeferenced points were sampled at irregular intervals. There were five plants in each sampled point; two pairs of leaves were removed from the lateral branches (3 rd and 4 th pairs from extremity to the basis) in the cardinal points of each plant, counting up 40 leaves per point. The foliar samples were chemically analyzed for the following nutrients: N, P, K, Ca, Mg, S, B, Cu and Zn. The same pattern of spatial dependence was presented with adjustment for K and B. Except for N and P, which presented random distribution, the other nutrients presented mild to severe spatial dependence justifying the geostatistical data analysis for making maps for differential and located, foliar and soil fertilizer application in coffee crop.

Evaluation of mechanical and thermal properties after chemical degradation of PVC and HDPE geomembranes

Chemical compatibility between geomembranes and site-specific waste liquids should be assessed since the waste liquids are highly complex mixtures. This paper presents some considerations about the chemical compatibility of geomembranes and some results of mechanical tests in HDPE and PVC geomembranes that were exposed to leachate and chemical residue (niobium). PVC and HDPE geomembranes of two thicknesses were tested: 1.0, 2.0 mm (PVC) and 0.8, 2.5 mm (HDPE). The results obtained show that after exposure the PVC geomembranes (1.0, 2.0 mm) were more rigid and stiffer than fresh samples. The HDPE geomembranes, on the other hand, when exposed to leachate and niobium residue presented increases in deformation. Melt flow index (MFI) tests were also carried out to verify the oxidation. © 2013 ejge.

Search for methylation-sensitive amplification polymorphisms in mutant figs

Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation in plants leads to gene repression, thereby affecting transcription without changing the DNA sequence. Previous studies using random amplification of polymorphic DNA and amplified fragment length polymorphism markers revealed no polymorphisms among select fig mutants that originated from gamma-irradiated buds. Therefore, we conducted methylation-sensitive amplified polymorphism analysis to verify the existence of variability due to epigenetic DNA methylation among these mutant selections compared to the main cultivar 'Roxo-de-Valinhos'. Samples of genomic DNA were double-digested with either HpaII (methylation sensitive) or MspI (methylation insensitive) and with EcoRI. Fourteen primer combinations were tested, and on an average, non-methylated CCGG, symmetrically methylated CmCGG, and hemimethylated hmCCGG sites accounted for 87.9, 10.1, and 2.0%, respectively. MSAP analysis was effective in detecting differentially methylated sites in the genomic DNA of fig mutants, and methylation may be responsible for the phenotypic variation between treatments. Further analyses such as polymorphic DNA sequencing are necessary to validate these differences, standardize the regions of methylation, and analyze reads using bioinformatic tools. © FUNPEC-RP.

A survey of DNA methylation across social insect species, life stages, and castes reveals abundant and caste-associated methylation in a primitively social wasp

DNA methylation plays an important role in the epigenetic control of developmental and behavioral plasticity, with connections to the generation of striking phenotypic differences between castes (larger, reproductive queens and smaller, non-reproductive workers) in honeybees and ants. Here, we provide the first comparative investigation of caste- and life stage-associated DNA methylation in several species of bees and vespid wasps displaying different levels of social organization. Our results reveal moderate levels of DNA methylation in most bees and wasps, with no clear relationship to the level of sociality. Strikingly, primitively social Polistes dominula paper wasps show unusually high overall DNA methylation and caste-related differences in site-specific methylation. These results suggest DNA methylation may play a role in the regulation of behavioral and physiological differences in primitively social species with more flexible caste differences. © 2013 Springer-Verlag Berlin Heidelberg.