Analysis of Respiratory Chain Regulation in Roots of Soybean Seedlings1

Changes in the respiratory rate and the contribution of the cytochrome (Cyt) c oxidase and alternative oxidase (COX and AOX, respectively) were investigated in soybean (Glycine max L. cv Stevens) root seedlings using the 18O-discrimination method. In 4-d-old roots respiration proceeded almost entirely via COX, but by d 17 more than 50% of the flux occurred via AOX. During this period the capacity of COX, the theoretical yield of ATP synthesis, and the root relative growth rate all decreased substantially. In extracts from whole roots of different ages, the ubiquinone pool was maintained at 50% to 60% reduction, whereas pyruvate content fluctuated without a consistent trend. In whole-root immunoblots, AOX protein was largely in the reduced, active form at 7 and 17 d but was partially oxidized at 4 d. In isolated mitochondria, Cyt pathway and succinate dehydrogenase capacities and COX I protein abundance decreased with root age, whereas both AOX capacity and protein abundance remained unchanged. The amount of mitochondrial protein on a dry-mass basis did not vary significantly with root age. It is concluded that decreases in whole-root respiration during growth of soybean seedlings can be largely explained by decreases in maximal rates of electron transport via COX. Flux via AOX is increased so that the ubiquinone pool is maintained in a moderately reduced state.

Clinical utility of ocular residual astigmatism and topographic disparity vector indexes in subclinical and clinical keratoconus

Purpose. We aimed to characterize the distribution of the vector parameters ocular residual astigmatism (ORA) and topography disparity (TD) in a sample of clinical and subclinical keratoconus eyes, and to evaluate their diagnostic value to discriminate between these conditions and healthy corneas. Methods. This study comprised a total of 43 keratoconic eyes (27 patients, 17–73 years) (keratoconus group), 11 subclinical keratoconus eyes (eight patients, 11–54 years) (subclinical keratoconus group) and 101 healthy eyes (101 patients, 15–64 years) (control group). In all cases, a complete corneal analysis was performed using a Scheimpflug photography-based topography system. Anterior corneal topographic data was imported from it to the iASSORT software (ASSORT Pty. Ltd), which allowed the calculation of ORA and TD. Results. Mean magnitude of the ORA was 3.23 ± 2.38, 1.16 ± 0.50 and 0.79 ± 0.43 D in the keratoconus, subclinical keratoconus and control groups, respectively (p < 0.001). Mean magnitude of the TD was 9.04 ± 8.08, 2.69 ± 2.42 and 0.89 ± 0.50 D in the keratoconus, subclinical keratoconus and control groups, respectively (p < 0.001). Good diagnostic performance of ORA (cutoff point: 1.21 D, sensitivity 83.7 %, specificity 87.1 %) and TD (cutoff point: 1.64 D, sensitivity 93.3 %, specificity 92.1 %) was found for the detection of keratoconus. The diagnostic ability of these parameters for the detection of subclinical keratoconus was more limited (ORA: cutoff 1.17 D, sensitivity 60.0 %, specificity 84.2 %; TD: cutoff 1.29 D, sensitivity 80.0 %, specificity 80.2 %). Conclusion. The vector parameters ORA and TD are able to discriminate with good levels of precision between keratoconus and healthy corneas. For the detection of subclinical keratoconus, only TD seems to be valid.

Reports of cases in the Supreme Court of Appeals of Virginia.

Some vols. also contain reports of cases in the General Court of Virginia.

Raccolta di tutti gli antichi poeti latini co la loro versione nell'italiana favella : tomo decimonono : contiene le prime tre commedie /

Vita di Publio Terenzio scritta dal signor d. Filippo Argelati ; Andria ; Heautontimoroumenos, ovvero Il punitore di se stesso ; Eunuco.

[Orationes M.T.C. dialectico et rhetorico artificio illustrata ... /

Contiene: Pro P. Quintio oratio ; Pro Sex. Roscio Amerino oratio ; Pro Q. Roscio comoedo oratio ; Accusationis in C. Verrem oratio ; Pro M. Fonteio oratio mutilata ; Pro A. Cecinna oratio.

In orationem M. T. Ciceronis pro C. Rabirio perduellionis reo Federici Ceruti Veronensis commentarius.

Edit16,

Lelio o Diálogo de Marco Tulio Cicerón sobre la amistad... seguida de algunos fragmentos de Séneca sobre la amistad... y de la refutación que hace Tulio en el libro de Finibus de la doctrina de Epicuro, aplicada a la amistad /

Mode of access: Internet.

M. Tullii Ciceronis Opera, cum delectu commentariorum edebat Josephus Olivetus ... ; tomus primus, quo rhetorica continentur.

Mode of access: Internet.

M. Tullii Ciceronis Opera, cum delectu commentariorum edebat Josephus Olivetus ... ; tomus octavus, in quo Epistolae ad Atticum.

Mode of access: Internet.

M.T. Ciceronis Opera omnia cum delectu commentariorum, in usum Serenissimi Delphini : tomus sextus qui De officiis, &c.

Mode of access: Internet.

Les livres de Ciceron de la viellesse, et de l'amitié / avec les paradoxes du même auteurs ; traduits en françois sur l'édition latine de Graevius, avec des notes, et des sommaires des chapitres ; par M. du Bois, ...

Mode of access: Internet.

De Partitione oratoria M. T. Ciceronis dialogus /

ICCU,

Rhetorica. Rhetoricorum ad C. Herennium lib. IIII. ; M. Tul. Ciceronis De inuentione lib. II. ; De oratore ad Quintum fratrem lib. III. ; De claris oratoribus, qui dicitur Brutus lib. I. Orator ad Brutum lib. I. ; Topica ad Trebatium lib. I. ; Oratoriae partitiones lib. I. ; Eiusdem de optimo genere oratorum praefatio quaedam ; Variae lectiones ad calcem reiectae.

Mode of access: Internet.

M. Tullii Ciceronis Opera : cum delectu commentariorum ... : tomus nonus, in quo Epistolae ad Q. Fratrem & c.

Mode of access: Internet.