The human SWI/SNF-B chromatin-remodeling complex is related to yeast Rsc and localizes at kinetochores of mitotic chromosomes

The SWI/SNF family of chromatin-remodeling complexes facilitates gene expression by helping transcription factors gain access to their targets in chromatin. SWI/SNF and Rsc are distinctive members of this family from yeast. They have similar protein components and catalytic activities but differ in biological function. Rsc is required for cell cycle progression through mitosis, whereas SWI/SNF is not. Human complexes of this family have also been identified, which have often been considered related to yeast SWI/SNF. However, all human subunits identified to date are equally similar to components of both SWI/SNF and Rsc, leaving open the possibility that some or all of the human complexes are rather related to Rsc. Here, we present evidence that the previously identified human SWI/SNF-B complex is indeed of the Rsc type. It contains six components conserved in both Rsc and SWI/SNF. Importantly, it has a unique subunit, BAF180, that harbors a distinctive set of structural motifs characteristic of three components of Rsc. Of the two mammalian ATPases known to be related to those in the yeast complexes, human SWI/SNF-B contains only the homolog that functions like Rsc during cell growth. Immunofluorescence studies with a BAF180 antibody revealed that SWI/SNF-B localizes at the kinetochores of chromosomes during mitosis. Our data suggest that SWI/SNF-B and Rsc represent a novel subfamily of chromatin-remodeling complexes conserved from yeast to human, and could participate in cell division at kinetochores of mitotic chromosomes.

Dynamics of Immature Secretory Granules: Role of Cytoskeletal Elements during Transport, Cortical Restriction, and F-Actin-dependent Tethering

Secretory granules store neuropeptides and hormones and exhibit regulated exocytosis upon appropriate cellular stimulation. They are generated in the trans-Golgi network as immature secretory granules, short-lived vesicular intermediates, which undergo a complex and poorly understood maturation process. Due to their short half-life and low abundance, real-time studies of immature secretory granules have not been previously possible. We describe here a pulse/chase-like system based on the expression of a human chromogranin B-GFP fusion protein in neuroendocrine PC12 cells, which permits direct visualization of the budding of immature secretory granules and their dynamics during maturation. Live cell imaging revealed that newly formed immature secretory granules are transported in a direct and microtubule-dependent manner within a few seconds to the cell periphery. Our data suggest that the cooperative action of microtubules and actin filaments restricts immature secretory granules to the F-actin-rich cell cortex, where they move randomly and mature completely within a few hours. During this maturation period, secretory granules segregate into pools of different motility. In a late phase of maturation, 60% of secretory granules were found to be immobile and about half of these underwent F-actin-dependent tethering.

The H+-Sucrose Cotransporter NtSUT1 Is Essential for Sugar Export from Tobacco Leaves1

In many species translocation of sucrose from the mesophyll to the phloem is carrier mediated. A sucrose/H+-symporter cDNA, NtSUT1, was isolated from tobacco (Nicotiana tabacum) and shown to be highly expressed in mature leaves and at low levels in other tissues, including floral organs. To study the in vivo function of NtSUT1, tobacco plants were transformed with a SUT1 antisense construct under control of the cauliflower mosaic virus 35S promoter. Upon maturation, leaves of transformants expressing reduced amounts of SUT1 mRNA curled downward, and strongly affected plants developed chloroses and necroses that led to death. The leaves exhibited impaired ability to export recently fixed 14CO2 and were unable to export transient starch during extended periods of darkness. As a consequence, soluble carbohydrates accumulated and photosynthesis was reduced. Autoradiographs of leaves show a heterogenous pattern of CO2 fixation even after a 24-h chase. The 14C pattern does not change with time, suggesting that movement of photosynthate between mesophyll cells may also be impaired. The affected lines show a reduction in the development of the root system and delayed or impaired flowering. Taken together, the effects observed in a seed plant (tobacco) demonstrate the importance of SUT1 for sucrose loading into the phloem via an apoplastic route and possibly for intermesophyll transport as well.

NMR studies of muscle glycogen synthesis in insulin-resistant offspring of parents with non-insulin-dependent diabetes mellitus immediately after glycogen-depleting exercise.

To examine the impact of insulin resistance on the insulin-dependent and insulin-independent portions of muscle glycogen synthesis during recovery from exercise, we studied eight young, lean, normoglycemic insulin-resistant (IR) offspring of individuals with non-insulin-dependent diabetes mellitus and eight age-weight matched control (CON) subjects after plantar flexion exercise that lowered muscle glycogen to approximately 25% of resting concentration. After approximately 20 min of exercise, intramuscular glucose 6-phosphate and glycogen were simultaneously monitored with 31P and 13C NMR spectroscopies. The postexercise rate of glycogen resynthesis was nonlinear. Glycogen synthesis rates during the initial insulin independent portion (0-1 hr of recovery) were similar in the two groups (IR, 15.5 +/- 1.3 mM/hr and CON, 15.8 +/- 1.7 mM/hr); however, over the next 4 hr, insulin-dependent glycogen synthesis was significantly reduced in the IR group [IR, 0.1 +/- 0.5 mM/hr and CON, 2.9 +/- 0.2 mM/hr; (P < or = 0.001)]. After exercise there was an initial rise in glucose 6-phosphate concentrations that returned to baseline after the first hour of recovery in both groups. In summary, we found that following muscle glycogen-depleting exercise, IR offspring of parents with non-insulin-dependent diabetes mellitus had (i) normal rates of muscle glycogen synthesis during the insulin-independent phase of recovery from exercise and (ii) severely diminished rates of muscle glycogen synthesis during the subsequent recovery period (2-5 hr), which has previously been shown to be insulin-dependent in normal CON subjects. These data provide evidence that exercise and insulin stimulate muscle glycogen synthesis in humans by different mechanisms and that in the IR subjects the early response to stimulation by exercise is normal.

Diminished degradation of yeast cytochrome c by interactions with its physiological partners.

The level and structure of yeast iso-1-cytochrome c and iso-2-cytochrome c, encoded by the nuclear genes CYC1 and CYC7, respectively, are normally not altered in rho- mutants, which completely lack the cytochromes a.a3 subunits and cytochrome b that are encoded by mitochondrial DNA. In contrast, iso-cytochromes c containing the amino acid change Thr-78-->Ile (T78I) were observed at the normal or near-normal wild-type level in rho+ strains but were completely absent in rho- mutants. We have demonstrated with the "global" suppressor mutation Asn-52-->Ile and by pulse-chase labeling that the T78I iso-1-cytochrome c undergoes rapid cellular degradation in rho- mutants. Furthermore, specific mutations revealed that the deficiency of T78I iso-1 cytochrome c can be caused by the lack of cytochrome a.a3 or cytochrome c1, but not by the lack of cytochrome b. Thus, this and certain other, but not all, labile forms of cytochrome c are protected from degradation by the interaction with its physiological partners.

Urban penalty: nuevas hip��tesis y caso espa��ol (1860-1920)

El t��rmino urban penalty fue en principio utilizado para definir la sobremortalidad urbana durante la revoluci��n industrial. Los antrop��metras lo emplearon m��s tarde para definir un hecho simult��neo: el descenso de la estatura urbana con respecto a la rural. En la primera parte del trabajo proponemos tres nuevas hip��tesis sobre ese castigo urbano: a) incorporar las aportaciones de la Teor��a Econ��mica sobre fallos de mercado al an��lisis de la sobremortalidad urbana; b) explicar la urban penalty mediante el modelo que Floud, Fogel, Harris y Chul Hong han elaborado recientemente para Gran Breta��a y c) sugerir respuestas a una pregunta que debe ser avalada o desmentida por investigaciones de ��mbito municipal: ��por qu�� los pol��ticos espa��oles de la restauraci��n tardaron d��cadas en acometer la reforma sanitaria de las ciudades? En la segunda parte del trabajo ofrecemos informaci��n que demuestra que Espa��a padeci�� sobremortalidad urbana derivada de fallos de mercado. Los datos de estatura rural y urbana evidencian por el contrario que, salvo excepciones, el pa��s no experiment�� urban penalty. El trabajo termina tratando de explicar esta particularidad y sosteniendo que el modelo de Floud, Fogel, Harris y Chul Hong puede aplicarse a esas excepciones.

Portland & Rochester Railroad, New England, 1860 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map showing the Portland & Rochester railroad and its connections, prepared by G.W. & C.B. Colton & Co. It was published in 1860. Scale [ca. 1:900,000]. Covers Vermont, New Hampshire, Massachusetts, Connecticut, Rhode Island, and portions of New York, Maine, and the provinces of Quebec and New Brunswick, Canada.The image inside the map neatline is georeferenced to the surface of the earth and fit to the USA Contiguous Albers Equal Area Conic projection (Meters). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as railraods, principal and proposed railroad connections, drainage, state, county, and town boundaries, and more. Relief shown by hachures. Includes table of distances and inset: [Northeastern United States]. Scale [ca. 1:7,600,000].This layer is part of a selection of digitally scanned and georeferenced historic maps of New England from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Saint Petersburg, Russia, 1860 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: [St. Petersburg = Sanktpeterburga], published under the superintendance of the Society for the Diffusion of Useful Knowledge ; drawn by W.B. Clarke ; engraved by B.R. Davies. It was published by Edward Stanford ca. 1860. Scale [ca. 1:31,000]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the 'Pulkovo 1995 Gauss Kruger Zone 6N' coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, drainage, built-up areas and selected buildings, ground cover, and more. Includes index and inset view: Isakiefskoi Bridge. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

H��dh�� Shar��� al-Faq��h al-��all��mah ... al-fahh��mah Sayyid Mu���ammad ibn al-���asan Aq���ab�� ��al�� Man�����mat al-Shaykh al-Im��m kh��timat jah��bidhat al-mu���aqqiq��n al-a��l��m Sayyid al-���ayyib Ibn K��r��n wa-bi-h��mishihi Shar��� al-Shaykh al-����lim al-b��ri�� ... Sayyid al-Tih��m�� al-B��r�� ��al�� al-Man�����mah al-madhk��rah ay���an

��qa���b��'s commentary of the Man�����mah of Ibn K��r��n, with al-Tih��m��'s commentary of the same Man�����mah in the margin.

Kit��b Naw��dir al-zam��n fi waq����i�� Jabal Lubn��n / ta��l��f Iskandar Afand�� Abk��riy��s : manuscript, 1877?

Text is a history of Lebanon in the early 20 century.

Evaluation of an extended aeration process for Skokomish salmon processing wastewater treatment /

Mode of access: Internet.

Notes on the planting of salmon and trout fry,

Cover-title.

Memoria en que el Ministro de la Confederaci��n Argentina en las c��rtes de Inglaterra, Francia y Espa��a da cuenta �� su gobierno de los trabajos de su misi��n, desde 1855 hasta 1860, con ocasi��n de la renuncia que hace de todos sus empleos.

Signed: Juan B. Alberdi.

Senators from Pennsylvania. Memorial addresses delivered in the Senate and House of Representatives of the United States in memory of Philander C. Knox, Boies Penrose, William E. Crow, late senators from Pennsylvania. Sixty-seventh Congress. Proceedings in the Senate, January 28, 1923. Proceedings in the House, February 18, 1923.

Running title: Memorial addresses.