Electrostatic interactions between rotor and stator in the bacterial flagellar motor

Bacterial flagellar motors rotate, obtaining power from the membrane gradient of protons or, in some species, sodium ions. Torque generation in the flagellar motor must involve interactions between components of the rotor and components of the stator. Sites of interaction between the rotor and stator have not been identified. Mutational studies of the rotor protein FliG and the stator protein MotA showed that both proteins contain charged residues essential for motor rotation. This suggests that functionally important electrostatic interactions might occur between the rotor and stator. To test this proposal, we examined double mutants with charged-residue substitutions in both the rotor protein FliG and the stator protein MotA. Several combinations of FliG mutations with MotA mutations exhibited strong synergism, whereas others showed strong suppression, in a pattern that indicates that the functionally important charged residues of FliG interact with those of MotA. These results identify a functionally important site of interaction between the rotor and stator and suggest a hypothesis for electrostatic interactions at the rotor–stator interface.

Engineering model of future motor vehicles. Volume I. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Engineering model of future motor vehicles. Volume II: data book. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Cost evaluation for nine Federal Motor Vehicle Safety Standards: Task VIII - FMVSS 301, fuel system integrity - determination of the model year when manufacturers made changes to meet Standard's 1976 requirements. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

A model state classified licensing program. Guidelines for motor vehicle administrators.

Mode of access: Internet.

Engineering model of future motor vehicles. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Operator, organizational, direct support, and general support maintenance manual : printing press, offset, web-fed, motor driven, 208V, 60 hertz, Hess and Barker model 3CX, FSN 3610-617-7566.

Includes index.

Direct and general support and depot maintenance repair parts and special tools lists : air conditioner, floor mounted, air cooled, electric motor driven, AC, 3 phase, 400 cycle, 38,000 Btu hr, 416 volt (Keco model F-0038), FSN 4120-066-8771 ....

"April 1969."

Organizational maintenance repair parts and special tools list : air conditioning units, electric motor driven, 9,000 Btu/hr, vertical compact, 115 volt, single phase, 50/60 cycle (Therm-Air Manufacturing Co. model CV-9-1-15-60) FSN 4120-935-1609; 9,000 Btu/hr, vertical compact, 230 volt, three phase, 50/60 cycle ....

"February 1970."

Organizational maintenance repair parts and special tools list : air conditioner, vertical, compact, self-contained, air cooled, electric motor driven, 115 volts, A.C. 50/60 hertz, single phase, 9,000 Btu/hr cooling, 6,000 Btu/hr heating (Harvey W. Hottel model CV-9-5/6-15), FSN 4120-935-1609.

"September 1974."

Hand receipt manual covering end item/components of end item (COEI), basic issue items (BII), and additional authorization list (AAL) related to compressor, reciprocating, air, receiver, mounted, electric motor driven, 5 cfm, 175 psi (Champion Pneumatic model 60CAW20HM-1) (national stock no. 4310-00-930-7147) ...

"February 1979."

Operator's, organizational, and direct support maintenance manual : tank and pump unit, liquid dispensing, for truck mounting, mil design tank and pump units : gasoline engine driven model (97403) 13217E7100, NSN 4930-00-426-9960 : electric motor driven model (97403) 13217E7130, NSN 4930-01-130-7281.

"17 July 1984."

Operator, organizational, direct support, and general support maintenance manual : motor generator, power supply generator, 60 kw, 120/208, 240/416 volt, 3-phase, 4 wire service, 400 hertz, DC component output, 28 volt, 10 amp, motor input AC, 416 volt, 3-phase, 60 hertz, portable skid mounted (General Dynamics Corp. model 14105008), FSN 6125-917-2244.

"June 1971."

Direct, general support, and depot maintenance manual : tractor, wheeled, warehouse, gasoline engine driven, 4-wheel, pneumatic tired, 4000 lb drawbar pull, Army model MHE 201 (Northwestern Motor Co. model JG 40PT4), FSN 3930-926-1066.

"December 1967."

Organizational maintenance repair parts and special tools lists : tractor, wheeled, warehouse, gasoline engine driven; 4-wheel, pneumatic tired, 4000 lb DBP, Army model MHE 201 (Northwestern Motor Co. model JG-40PT4), FSN 3930-926-1066.

"November 1968."