342 resultados para K1


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Relacion de las fiestas que la ... Villa de Madrid, hizo en la coronizacion de... San Isidro ... : p. VII-LXXIV, con port. propia ; La niñez de San Isidro ... : p. 1-74 ; La juventud de San Isidro ... : p. 75-148 ; Justa poética ... : p. 149-426.

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"Premier partie. Chimie" ; "Seconde partie. Métallurgie "

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Barbier 1-802, BNP 8-753, NUC B-186198.

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Mode of access: Internet.

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Mode of access: Internet.

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Contiene con portada propia: "Praelectiones chymicae, in quibus omnes fere operationes chymicae ad vera principia, & ipsius naturae leges rediguntur", [2], xij, [2], 102 p. -- "Appendix in qua critica exhibetur harum praelectionum relatio in actis lipsisiensibus edita", 31 p.

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Stable expression of human groups IIA and X secreted phospholipases A(2) (hGIIA and hGX) in CHO-K1 and HEK293 cells leads to serum- and interleukin-1beta-promoted arachidonate release. Using mutant CHO-K1 cell lines, it is shown that this arachidonate release does not require heparan sulfate proteoglycan- or glycosylphosphatidylinositol-anchored proteins. It is shown that the potent secreted phospholipase A(2) inhibitor Me-Indoxam is cell-impermeable. By use of Me-Indoxam and the cell-impermeable, secreted phospholipase A(2) trapping agent heparin, it is shown that hGIIA liberates free arachidonate prior to secretion from the cell. With hGX-transfected CHO-K1 cells, arachidonate release occurs before and after enzyme secretion, whereas all of the arachidonate release from HEK293 cells occurs prior to enzyme secretion. Immunocytochemical studies by confocal laser and electron microscopies show localization of hGIIA to the cell surface and Golgi compartment. Additional results show that the interleukin-1beta-dependent release of arachidonate is promoted by secreted phospholipase A(2) expression and is completely dependent on cytosolic (group IVA) phospholipase A(2). These results along with additional data resolve the paradox that efficient arachidonic acid release occurs with hGIIA-transfected cells, and yet exogenously added hGIIA is poorly able to liberate arachidonic acid from mammalian cells.