Images of a Broken World

Thesis (Master's)--University of Washington, 2016-08

California Calling

Thesis (Master's)--University of Washington, 2016-06

Intersectionality in the Language and Writing Classroom

Thesis (Ph.D.)--University of Washington, 2016-06

A comprehensive analysis of sexual dimorphism in the midbrain dopamine system

Thesis (Ph.D.)--University of Washington, 2016-06

Echocardiographic parameters in 14 healthy English Bull Terriers

Objective To determine the range of various cardiac parameters using echocardiography in apparently normal, healthy English Bull Terriers. Design Fourteen English Bull Terriers were selected for study. Cardiac auscultation of the parents of these dogs was normal. Echocardiographic examination of one parent of each animal showed: no mitral or aortic valve abnormalities; no myocardial lesions; no two dimensional evidence of fixed or dynamic left ventricular outflow tract obstruction; and no systolic aortic or left ventricular outflow tract turbulence on colour flow Doppler examination. The 14 selected dogs did not have arrhythmias or murmurs, and on echocardiographic examination had similar findings to their parents. Systolic blood pressure was measured in all dogs and they had no clinical evidence of Bull Terrier polycystic kidney disease or Bull Terrier hereditary nephritis. Procedure All dogs were auscultated and subjected to a sequential global echocardiographic assessment of the heart, including two dimensional long and short axis, and colour flow Doppler interrogation of the mitral and aortic valves. Dimensional measurements, including those from the left atrium, aortic annulus and left ventricle, were taken from a right parasternal window, and derived values such as fractional shortening, stroke volume and left atrial to aortic annulus ratio were calculated. Peak systolic aortic velocity was measured from the left parasternal window using two dimensional-guided pulsed wave Doppler with angle correction. Systolic blood pressure was measured using a Doppler monitor. The absence of Bull Terrier polycystic kidney disease was determined using renal ultrasonography, and of Bull Terrier hereditary nephritis using urinary protein to creatinine ratio. Results These 14 dogs had greater left ventricular wall thickness and smaller aortic root diameters than those reported as normal for other breeds of comparable body size. Left atrial dimensions were also larger, however this may have been due to the maximising method of measurement. These apparently normal English Bull Terriers also had higher aortic velocities than those reported for other breeds, possibly due to a smaller aortic root diameter or other anatomic substrate of the left ventricular outflow tract, lower systemic vascular resistance, or breed-specific normal left ventricular hypertrophy. While these dogs were selected to be as close to normal as possible, the breed may have a particular anatomy that produces abnormal left ventricular echocardiographic parameters. Conclusion These echocardiographic parameters may be used to diagnose left ventricular outflow tract obstruction and left ventricular hypertrophy, and inaccurate diagnoses may result if breed-specific values are not used.

mecA locus diversity in methicillin-resistant Staphylococcus aureus isolates in Brisbane, Australia, and the development of a novel diagnostic procedure for the Western Samoan phage pattern clone

An emerging public health phenomenon is the increasing incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections that are acquired outside of health care facilities. One lineage of community-acquired MRSA (CA-MRSA) is known as the Western Samoan phage pattern (WSPP) clone. The central aim of this study was to develop an efficient genotyping procedure for the identification of WSPP isolates. The approach taken was to make use of the highly variable region downstream of mecA in combination with a single nucleotide polymorphism (SNP) defined by the S. aureus multilocus sequence typing (MLST) database. The premise was that a combinatorial genotyping method that interrogated both a highly variable region and the genomic backbone would deliver a high degree of informative power relative to the number of genetic polymorphisms-interrogated. Thirty-five MRSA isolates were used for this study, and their gene contents and order downstream of mecA were determined. The CA-MRSA isolates were found to contain a truncated mecA downstream region consisting of mecA-HVR-IS431 mec-dcs-Ins117, and a PCR-based method for identifying this structure was developed. The hospital-acquired isolates were found to contain eight different mecA downstream regions, three of which were novel. The Minimum SNPs computer software program was used to mine the S. aureus MLST database, and the arcC 2726 polymorph was identified as 82% discriminatory for ST-30. A real-time PCR assay was developed to interrogate this SNP. We found that the assay for the truncated mecA downstream region in combination with the interrogation of arcC position 272 provided an unambiguous identification of WSPP isolates.

Transcriptome analysis of Neotyphodium and Epichloë grass endophytes

Large-scale gene discovery has been performed for the grass fungal endophytes Neotyphodium coenophialum, Neotyphodium lolii, and Epichloe festucae. The resulting sequences have been annotated by comparison with public DNA and protein sequence databases and using intermediate gene ontology annotation tools. Endophyte sequences have also been analysed for the presence of simple sequence repeat and single nucleotide polymorphism molecular genetic markers. Sequences and annotation are maintained within a MySQL database that may be queried using a custom web interface. Two cDNA-based microarrays have been generated from this genome resource, They permit the interrogation of 3806 Neotyphodium genes (Nchip (TM) rnicroarray), and 4195 Neotyphodium and 920 Epichloe genes (EndoChip (TM) microarray), respectively. These microarrays provide tools for high-throughput transcriptome analysis, including genome-specific gene expression studies, profiling of novel endophyte genes, and investigation of the host grass-symbiont interaction. Comparative transcriptome analysis in Neotyphodium and Epichloe was performed. (c) 2006 Elsevier

No pain, no gain? Torture and ethics in the war on terror

Is the use of torture ever justified? This article argues that torture cannot be justified, even in so called ticking bomb cases, but that in such extreme situations it may be necessary. In those situations, judgements about whether the use of torture is legitimate must balance the imminence and gravity of the threat with the need to prevent future occurrences of torture and maintain a normative environment that is hostile to its use. The article begins by observing that the use of torture and/or cruel and degrading treatment has become a core component of the global war on terror. It tests the claim that the use of coercive interrogation techniques does not constitute torture, showing that similar arguments were levelled by both the British and French governments in relation to Northern Ireland and Algeria respectively and found wanting. It then evaluates and rejects Dershowitz's claim for the legalization of torture and the more limited claim that torture may be permissible in ticking bomb scenarios. In the final section, the article questions how we might maintain the prohibition on torture while acknowledging that it may be necessary in some hypothetical cases.

AVALIAÇÃO EM MATEMÁTICA NA EDUCAÇÃO BÁSICA, DO ESTADO DE SÃO PAULO: PONTOS DE VISTA DOS SUJEITOS ENVOLVIDOS

Este trabalho tem por objetivo levantar e analisar algumas concepções sobre a avaliação escolar em Matemática e sobre a Avaliação em Matemática do Sistema de Avaliação de Rendimento Escolar do Estado de São Paulo (SARESP), procurando desvendar a realidade que é encontrada nas escolas paulistas referente à avaliação dessa disciplina. O intuito é verificar os pontos de vista dos alunos, professores, gestores escolares, família e especialistas para articular uma organização escolar que desenvolva um processo permanente de construção do conhecimento. Para verificar quais as concepções referentes às avaliações já citadas foram adotadas entrevistas, questionários e desenhos realizados pelos alunos, mostrando o que pensam sobre as avaliações previamente aplicadas. As entrevistas somente foram aplicadas aos discentes, pois seus depoimentos são fundamentais para a pesquisa. Para a concretização destas entrevistas foi solicitado aos alunos à produção de desenhos que representassem o que são as avaliações já mencionadas anteriormente. Levantamos uma grande quantidade de informações a partir desses depoimentos e desenhos sobre as avaliações pesquisadas, a opinião que possuem sobre a escola, seus professores de Matemática, de como são ministradas as aulas e também sobre a forma de avaliar dos professores. Os questionários foram dirigidos ao corpo docente de Matemática, diretor, vice-diretor, professor coordenador, supervisor de ensino, família e especialistas em Educação, Psicologia da Educação e Matemática para verificar também sua concepção sobre avaliação escolar em Matemática e sobre a prova de Matemática do SARESP, que fazem parte fundamental de todo o processo de avaliação, pois, direta ou indiretamente, estão ligados a ela e são responsáveis pelos seus resultados. Na análise dos dados obtidos foram destacados, nos depoimentos dos questionários e das entrevistas, as,unidades significativas referentes aos processos de avaliações pesquisadas, para com isso analisarmos e verificarmos quais são as opiniões e fatos que podem ajudar ou atrapalhar todo o processo de Avaliação em Matemática, tanto o escolar quanto o de grande escala como o SARESP. Também foram analisados o que os alunos representaram na produção dos desenhos. As imagens adquiridas demonstraram muitas informações sobre o que os discentes pensam sobre a Avaliação em Matemática ao desenhar monstros, corações, números e até mesmo pontos de exclamação e de interrogação. Este estudo foi desenvolvido em uma escola pública da rede Estadual de ensino do Estado de São Paulo. Essa pesquisa investiga o que realmente está acontecendo em todo o processo de Avaliação em Matemática, possibilitando identificar no que cada sujeito envolvido neste processo pode contribuir ou atrapalhar para sua concretização, evidenciando assim áreas críticas efavoráveis sobre a tão temida e polêmica Avaliação em Matemática.

The social cognitive neuroscience of organisations

Social cognitive neuroscience is an emerging branch of cognitive neuroscience that bridges together social psychology and neuroscience. At its core is an understanding of the relationship between the brain and social interaction. The social cognitive neuroscientist places empirical endeavor within a three–stage framework, and questions falling under the SCN rubric undergo interrogation at each of these three levels. Firstly, we seek to understand a neuroscience of social interactions at the social level. Here we need to understand the motivational and other social factors that drive our behavior and experience in the real world. It goes without saying that any study of the cognitive neuroscience of socially interactive behavior must first be informed by social psychological theory to maintain ecological validity. Second, the social cognitive neuroscientist must be an adroit cognitive psychologist and be able to examine interactive behavior from the cognitive level. It is here that information–processing models and theories are applied to the understanding of our social behavior. Finally, studies at the neural level seek to inform us about the cortical structures, as well as the way they interact with other, in the mediation of the previous cognitive level. This volume brings together contributions from leading thinkers in both the social cognitive neurosciences and business to provide a comprehensive introduction and overview of a social cognitive neuroscience of the business brain. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full–text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member

Proteomics in the study of hippocampal plasticity

Synaptic plasticity is the dynamic regulation of the strength of synaptic communication between nerve cells. It is central to neuronal development as well as experience-dependent remodeling of the adult nervous system as occurs during memory formation. Aberrant forms of synaptic plasticity also accompany a variety of neurological and psychiatric diseases, and unraveling the biological basis of synaptic plasticity has been a major goal in neurobiology research. The biochemical and structural mechanisms underlying different forms of synaptic plasticity are complex, involving multiple signaling cascades, reconfigurations of structural proteins and the trafficking of synaptic proteins. As such, proteomics should be a valuable tool in dissecting the molecular events underlying normal and disease-related forms of plasticity. In fact, progress in this area has been disappointingly slow. We discuss the particular challenges associated with proteomic interrogation of synaptic plasticity processes and outline ways in which we believe proteomics may advance the field over the next few years. We pay particular attention to technical advances being made in small sample proteomics and the advent of proteomic imaging in studying brain plasticity.

Wavelength-division and spatial multiplexing using tandem interferometers for Bragg grating sensor networks

We present a new method for the interrogation of large arrays of Bragg grating sensors. Eight gratings operating between the wavelengths of 1533 and 1555 nm have been demultiplexed. An unbalanced Mach—Zehnder interferometer illuminated by a single low-coherence source provides a high-phase-resolution output for each sensor, the outputs of which are sequentially selected in wavelength by a tunable Fabry-Perot interferometer. The minimum detectable strain measured was 90 ne-vHz at 7 Hz for a wavelength of 1535 nm.

In-fibre directional transverse loading sensor based on excessively tilted fibre Bragg gratings

We report a distinctive polarization mode coupling behaviour of tilted fibre Bragg gratings (TFBGs) with a tilted angle exceeding 45°. The ex-45° TFBGs exhibit pronounced polarization mode splitting resulted from the birefringence induced by the grating structure asymmetry. We have fabricated TFBGs with a tilted structure at 81° and studied their properties under transverse load applied to their equivalent fast and slow axes. The results show that the light coupling to the orthogonally polarized modes of the 81°-TFBGs changes only when the load is applied to their slow axis, giving a prominent directional loading response. For the view of real applications, we further investigated the possibility of interrogating such a TFBG-based load sensor using low-cost and compact-size single wavelength source and power detector. The experimental results clearly show that the 81°-TFBGs plus the proposed power-measurement interrogation scheme may be developed to an optical fibre vector sensor system capable of not just measuring the magnitude but also recognizing the direction of the applied transverse load. Using such an 81°-TFBG based load sensor, a load change as small as 1.6 × 10-2 g may be detected by employing a standard photodiode detector.

A high sensitivity refractometer based upon a long period grating Mach-Zehnder interferometer

A low cost interrogation scheme is demonstrated for a refractometer based on an in-line fiber long period grating (LPG) Mach–Zehnder interferometer. Using this interrogation scheme the minimum detectable change in refractive index of ?n ~ 1.8×10-6 is obtained, which is the highest resolution achieved using a fiber LPG device, and is comparable to precision techniques used in the industry including high performance liquid chromatography and ultraviolet spectroscopy.

High birefringence fibre interrogating interferometer for optical sensing applications

The use of high birefringence fibre forming a differential path interferometer as an interrogation unit for heterodyne fibre optic sensing applications is described.