Diseño de un programa de seguridad y salud ocupacional para prevenir riesgos profesionales en la Asociación Cooperativa de Producción Agropecuaria de Ciudad Barrios de R.L., municipio de Ciudad Barrios, departamento de San Miguel año 2014

Para la realización del Programa de Seguridad y Salud Ocupacional, se efectuó un estudio de campo en la Cooperativa, utilizando como herramienta la encuesta y entrevista con el objetivo de elaborar un diagnóstico, el cual permitió conocer las actividades diarias de los miembros de la Asociación, y así mismo identificar cada una de las áreas de trabajo existentes. Para la realización del Programa de Seguridad y Salud Ocupacional se utilizaron dos métodos que permitieron evaluar cada una de las áreas y puestos de trabajo entre los métodos utilizados: El método William T. Fine que a través de las fichas de inspección permitió identificar y evaluar detalladamente cada una de los apartados establecidos en la Ley General de Prevención de Riesgos y cada uno de los puestos de trabajo arrojando como resultado el grado de peligrosidad existente en cada una de las áreas estudiadas; el método de Evaluación de Factores Psicosociales F-PSICO, a través de su encuesta permitió evaluar exclusivamente los aspectos psicosociales a cada uno de los empleados y así determinar cómo es el clima laboral que se vive. Se propone una serie de medidas preventivas por cada puesto de trabajo, es decir, acciones que debe realizar el trabajador para prevenir en gran manera los accidentes de trabajo y las enfermedades profesionales al momento de ejecutar las actividades encomendadas; así mismo se propone la señalización de seguridad a implementarse en la Asociación, la cual tiene la finalidad de indicar las acciones a seguir o alertar de peligro existente en determinada área de trabajo, del mismo modo se especifica el tipo de señalización, la dimensión y ubicación de la misma de modo que el personal pueda visualizar y comprender el mensaje que indica la señal y prevenir un riesgo profesional oportunamente.

Development of single nucleotide polymorphism (SNP) markers from the mango (Mangifera indica) transcriptome for mapping and estimation of genetic diversity

The development of molecular markers for genomic studies in Mangifera indica (mango) will allow marker-assisted selection and identification of genetically diverse germplasm, greatly aiding mango breeding programs. We report here our identification of thousands of unambiguous molecular markers that can be easily assayed across genotypes of the species. With origin centered in Southeast Asia, mangos are grown throughout the tropics and subtropics as a nutritious fruit that exhibits remarkable intraspecific phenotypic diversity. With the goal of building a high density genetic map, we have undertaken discovery of sequence variation in expressed genes across a broad range of mango cultivars. A transcriptome sequence reference was built de novo from extensive sequencing and assembly of RNA from cultivar 'Tommy Atkins'. Single nucleotide polymorphisms (SNPs) in protein coding transcripts were determined from alignment of RNA reads from 24 mango cultivars of diverse origins: 'Amin Abrahimpur' (India), 'Aroemanis' (Indonesia), 'Burma' (Burma), 'CAC' (Hawaii), 'Duncan' (Florida), 'Edward' (Florida), 'Everbearing' (Florida), 'Gary' (Florida), 'Hodson' (Florida), 'Itamaraca' (Brazil), 'Jakarata' (Florida), 'Long' (Jamaica), 'M. Casturi Purple' (Borneo), 'Malindi' (Kenya), 'Mulgoba' (India), 'Neelum' (India), 'Peach' (unknown), 'Prieto' (Cuba), 'Sandersha' (India), 'Tete Nene' (Puerto Rico), 'Thai Everbearing' (Thailand), 'Toledo' (Cuba), 'Tommy Atkins' (Florida) and 'Turpentine' (West Indies). SNPs in a selected subset of protein coding transcripts are currently being converted into Fluidigm assays for genotyping of mapping populations and germplasm collections. Using an alternate approach, SNPs (144) discovered by sequencing of candidate genes in 'Kensington Pride' have already been converted and used for genotyping.

O impacto da rede viária no uso do espaço da geneta (Genetta genetta L., 1758)

O presente estudo foi realizado numa área com 156,5 Km/ localizada nos municípios de Évora e Montemor-o-Novo, a Sul de Portugal. Cinco genetas foram seguidas via telemetria durante seis meses visando o estudo do uso do espaço e comportamento relativamente às estradas. Estas, parecem funcionar como limites das áreas vitais da geneta actuando como barreira física e/ou social aos seus movimentos. Contudo, as genetas cruzaram diferentes tipos de estrada embora com frequência reduzida. Por outro lado, as genetas adoptaram uma distância de segurança média das estradas, de 1km, onde se verifica uma aproximação durante a noite e um distanciamento durante o dia. Todos os animais evidenciaram ter uma distribuição não aleatória relativamente às estradas concentrando-se maioritariamente apenas num dos lados da estradão que indica o efeito destas infra-estruturas como barreiras parciais ao movimento. A presença de galerias ripicolas parece promover o uso das bermas e durante o estudo todos os indivíduos seleccionaram positivamente estes habitats. ABSTRACT: The present study, was conducted in a 156,5 Km 2 area, located in the Évora and Montemor-o-Novo counties, southern Portugal. Five genets were radio-tracked for six months with the purpose of studying their space use and behavior towards roads. ln this study, roads seem to be artificial boundaries to genets' home ranges acting as physical and social barrier to their movements. However, genets have crossed different types of roads, although in lower frequencies. They adopted, in average, a security distance of 1km from roads being further during the day and closer at night. Ali animals showed a not random distribution on their activity concerning roads presence and their localizations tend to concentrate on one side of the road which confirms that these infrastructures pose a significant barrier to genets' movements. The existence of riparian galleries seems to promote roadside usage and during the study genets have selected positively these habitats.

Germinação de sementes e otimização de técnicas de micropropagação de umbuzeiro (spondias tuberosa, arr.) anacardiaceae

The brazilian-plum (Spondias tuberosa, His) is a tropical fruit tree that has been consolidated in the market for agribusiness processing, due to its characteristic flavor of fruit. Accordingly, studies to optimize the propagation of plants are necessary for production of seedlings with agronomic and quality assurance measures. This study aimed at determining the efficient techniques for uniform seed germination, as brazilian-plum seed present mechanical dormancy, and establish optimal culture media for multiplication of shoots from the in vitro micropropagation. Firstly, in a greenhouse at the Universidade Federal do Rio Grande do Norte, was evaluated the influence of different methods of breaking dormancy in the emergence of seedlings of brazilian-plum and speed of germination (IVG) of seeds. After 60 days of cultivation, it was found that splay in the distal portion of the seed was the best treatment, with rates of 85.33% in germinability and 3.415 of IVG, compared with the treatment of seed-soaking in water for 12h + humus and the control group. Subsequently, new sources of seedling explants were obtained in studies of tissue culture. Laboratory of Plant Biotechnology that the university, was used stem apex, nodal segments and internodes in search of decontamination with various concentrations of calcium hypochlorite [Ca(OCl)2] and micropropagation, inoculating them in half WPM (1980) with various concentrations of 6-benzylaminopurine (BAP). We used 10 sample units with three replications for different concentrations of [Ca(OCl)2], BAP and explants type. After thirty days, which was observed for the control of contamination, during the establishment in vitro, concentrations of [Ca(OCl)2] between 0.5% and 2.0% were effective in combating exogenous contamination of the apex. In nodal segments and internodes, concentrations of [Ca(OCl)2] between 1.0% and 2.0% and 1.5% and 2.0% were respectively, sufficient to reduce the percentage of losses in these infestations explants. For micropropagation, the culture medium supplemented with 0.1 mg.L-1 BAP promotes better development of multiple shoots per explants from nodal segment. However, success does not get to shoot training in internodal segment

Óleo de nim (Azadirachta indica) estimula o crescimento micelial de agentes de biocontrole.

2008

Análise do crescimento e desenvolvimento de acessos de taperebazeiro (Spondias mombim L.), do banco ativo de germoplasma da Embrapa Amazônia Oriental, em Belém-PA.

O presente trabalho teve como objetivo avaliar o desenvolvimento vegetativo das plantas do Banco Ativo de Germoplasma do Taperebazeiro da Embrapa Amazônia Oriental, onde se encontram 29 acessos de Spondias mombim procedentes de diversas localidades do Estado do Pará. Foram analisados, no período de cinco anos (2011 a 2015), o crescimento das plantas em altura (m), desenvolvimento do diâmetro do enxerto (cm) e o desenvolvimento do diâmetro do porta-enxerto (cm). Utilizou-se o delineamento inteiramente ao acaso, com 29 tratamentos e cinco repetições. As médias dos acessos foram comparadas pelo método de Scott-Knott. O acesso 14 foi o que apresentou maior desenvolvimento em altura (4,51m), porém sem diferir de outros 13 acessos. Também obteve melhor desempenho quanto ao crescimento transversal (diâmetros do enxerto e porta-enxerto), sem diferir, entretanto, de outros nove acessos. No geral, a relação do desenvolvimento vegetativo dos enxertos com os porta-enxertos foi alta (0,8), indicando boa compatibilidade, apesar de haver diferenças entre os acessos captadas pela análise.

Levantamento florístico associado a indivíduos de Schinopsis brasiliensis Engl. (Anacardiaceae) em diferentes ambientes.

Esse trabalho tem por objetivo realizar o levantamento florístico associado a indivíduos de Schinopsis brasiliensis, em diferentes ambientes, visando verificar a influência da situação de campo nessa associação. O levantamento foi realizado no Campo Experimental da Caatinga, pertencente à Embrapa Semiárido, em três áreas com diferentes ambientes. Em cada área, foram selecionados dez indivíduos de Schinopsis brasiliensis. O levantamento florístico foi realizado em cada individuo, registrando-se todas as espécies, com DNS menor que 3,0 cm, encontradas no limite estabelecido pelo diâmetro de copa. Foram registradas 76 espécies pertencentes a 34 famílias botânicas, sendo Euphorbiaceae (14,47%), Fabaceae (10,53%) e Poaceae (9,09%) as que se destacaram. O maior número de espécies associadas a indivíduos de S. brasilienses foi registrado na Caatinga em regeneração (n=51), seguida pela Caatinga preservada (n=43) e plantio adensado (n=33), mostrando que o manejo pode estar refletindo na diversidade de plantas encontrada sob a copa das árvores dessa espécie.

Occurrence Of Isoflavonoids In Brazilian Common Bean Germplasm (phaseolus Vulgaris L.).

Common bean (Phaseolus vulgaris) is present in the daily diet of various countries and, as for other legumes, has been investigated for its nutraceutical potential. Thus, 16 genotypes from different gene pools, representing seven types of seed coats and different responses to pathogens and pests, were selected to verify their isoflavone contents. The isoflavonoids daidzein and genistein and the flavonols kaempferol, myricetin, and quercetin were found. Grains of the black type showed the highest concentrations of isoflavonoids and were the only ones to exhibit daidzein. IAC Formoso, with high protein content and source of resistance to anthracnose, showed the greatest concentration of genistein, representing around 11% of the content present in soybean, as well as high levels of kaempferol. Arc 1, Raz 55, and IAC Una genotypes showed high content of coumestrol. The results suggest the use of IAC Formoso to increase the nutraceutical characteristics in common bean.

Influence Of The Stage Of Ripeness On The Composition Of Iridoids And Phenolic Compounds In Genipap (genipa Americana L.).

Genipap fruits, native to the Amazon region, were classified in relation to their stage of ripeness according to firmness and peel color. The influence of the part of the genipap fruit and ripeness stage on the iridoid and phenolic compound profiles was evaluated by HPLC-DAD-MS(n), and a total of 17 compounds were identified. Geniposide was the major compound in both parts of the unripe genipap fruits, representing >70% of the total iridoids, whereas 5-caffeoylquinic acid was the major phenolic compound. In ripe fruits, genipin gentiobioside was the major compound in the endocarp (38%) and no phenolic compounds were detected. During ripening, the total iridoid content decreased by >90%, which could explain the absence of blue pigment formation in the ripe fruits after their injury. This is the first time that the phenolic compound composition and iridoid contents of genipap fruits have been reported in the literature.

Auxin And Physical Constraint Exerted By The Perianth Promote Androgynophore Bending In Passiflora Mucronata L. (passifloraceae).

The androgynophore column, a distinctive floral feature in passion flowers, is strongly crooked or bent in many Passiflora species pollinated by bats. This is a floral feature that facilitates the adaptation to bat pollination. Crooking or bending of plant organs are generally caused by environmental stimulus (e.g. mechanical barriers) and might involve the differential distribution of auxin. Our aim was to study the role of the perianth organs and the effect of auxin in bending of the androgynophore of the bat-pollinated species Passiflora mucronata. Morpho-anatomical characterisation of the androgynophore, including measurements of curvature angles and cell sizes both at the dorsal (convex) and ventral (concave) sides of the androgynophore, was performed on control flowers, flowers from which perianth organs were partially removed and flowers treated either with auxin (2,4-dichlorophenoxyacetic acid; 2,4-D) or with an inhibitor of auxin polar transport (naphthylphthalamic acid; NPA). Asymmetric growth of the androgynophore column, leading to bending, occurs at a late stage of flower development. Removing the physical constraint exerted by perianth organs or treatment with NPA significantly reduced androgynophore bending. Additionally, the androgynophores of plants treated with 2,4-D were more curved when compared to controls. There was a larger cellular expansion at the dorsal side of the androgynophores of plants treated with 2,4-D and in both sides of the androgynophores of plants treated with NPA. This study suggests that the physical constraint exerted by perianth and auxin redistribution promotes androgynophore bending in P. mucronata and might be related to the evolution of chiropterophily in the genus Passiflora.

Supercritical Carbon Dioxide Extraction Of Capsaicinoids From Malagueta Pepper (capsicum Frutescens L.) Assisted By Ultrasound.

Extracts from malagueta pepper (Capsicum frutescens L.) were obtained using supercritical fluid extraction (SFE) assisted by ultrasound, with carbon dioxide as solvent at 15MPa and 40°C. The SFE global yield increased up to 77% when ultrasound waves were applied, and the best condition of ultrasound-assisted extraction was ultrasound power of 360W applied during 60min. Four capsaicinoids were identified in the extracts and quantified by high performance liquid chromatography. The use of ultrasonic waves did not influence significantly the capsaicinoid profiles and the phenolic content of the extracts. However, ultrasound has enhanced the SFE rate. A model based on the broken and intact cell concept was adequate to represent the extraction kinetics and estimate the mass transfer coefficients, which were increased with ultrasound. Images obtained by field emission scanning electron microscopy showed that the action of ultrasonic waves did not cause cracks on the cell wall surface. On the other hand, ultrasound promoted disturbances in the vegetable matrix, leading to the release of extractable material on the solid surface. The effects of ultrasound were more significant on SFE from larger solid particles.

Improvement Of The Physical Performance Is Associated With Activation Of No/pgc-1α/mttfa Signaling Pathway And Increased Protein Expressions Of Electron Transport Chain In Gastrocnemius Muscle From Rats Supplemented With L-arginine.

To examine the influence of l-arginine supplementation in combination with physical training on mitochondrial biomarkers from gastrocnemius muscle and its relationship with physical performance. Male Wistar rats were divided into four groups: control sedentary (SD), sedentary supplemented with l-arginine (SDLA), trained (TR) and trained supplemented with l-arginine (TRLA). Supplementation of l-arginine was administered by gavage (62.5mg/ml/day/rat). Physical training consisted of 60min/day, 5days/week, 0% grade, speed of 1.2km/h. The study lasted 8weeks. Skeletal muscle mitochondrial enriched fraction as well as cytoplasmic fractions were obtained for Western blotting and biochemical analyses. Protein expressions of transcriptor coactivator (PGC-1α), transcriptor factors (mtTFA), ATP synthase subunit c, cytochrome oxidase (COXIV), constitutive nitric oxide synthases (eNOS and nNOS), Cu/Zn-superoxide dismutase (SOD) and manganese-SOD (Mn-SOD) were evaluated. We also assessed in plasma: lipid profile, glycemia and malondialdehyde (MDA) levels. The nitrite/nitrate (NOx(-)) levels were measured in both plasma and cytosol fraction of the gastrocnemius muscle. 8-week l-arginine supplementation associated with physical training was effective in promoting greater tolerance to exercise that was accompanied by up-regulation of the protein expressions of mtTFA, PGC-1α, ATP synthase subunit c, COXIV, Cu/Zn-SOD and Mn-SOD. The upstream pathway was associated with improvement of NO bioavailability, but not in NO production since no changes in nNOS or eNOS protein expressions were observed. This combination would be an alternative approach for preventing cardiometabolic diseases given that in overt diseases a profound impairment in the physical performance of the patients is observed.

Otimização do processo de extração e isolamento do antimalárico artemisinina a partir de Artemisia annua L.

Malaria is still one of the major diseases in the world, causing physical and economic problems in tropical regions. Artemisinin (Qinghaosu), a natural compound identified in Artemisia annua L. , is an effective drug mainly against cerebral malaria. The action of this drug is immediate and parasitaemia in the treatment of drug-resistant malaria is rapidily reduced, justifying the industrial production of artemisinin. This article focuses on the industrial production of this potent antimalarial drug, including strategies for enhancing yield using inexpensive and easy steps.

Synthesis, characterization, DFT and Td-dfT study of the [Fe(mnt)(L)(t-BuNC) 2] octahedral complex (L = phen, bipy)

FeBr2 has reacted with an equivalent of mnt2- (mnt = cis-1,2-dicyanoethylene-1,2-dithiolate) and the α-diimine L (L = 1,10'-phenantroline, 2,2'-bipyridine) in THF solution, and followed by adding of t-butyl-isocyanide to give [Fe(mnt)(L)(t-BuNC)2] neutral compound. The products were characterized by infrared, UV-visible and Mössbauer spectroscopy, besides thermogravimetric and conductivity data. The geometry in the equilibrium was calculated by the density functional theory and the electronic spectrum by the time-dependent. The experimental and theoretical results in good agreement have defined an octahedral geometry with two isocyanide neighbours. The π→π* intraligand electronic transition was not observed for cis-isomers in the near-IR spectral region.

Desenvolvimento e caracterização de nanocápsulas de poli (L-lactídeo) contendo benzocaína

In this paper we describe the preparation poly (L-lactide) (PLA) nanocapsules as a drug delivery system for the local anesthetic benzocaine. The characterization and in vitro release properties of the system were investigated. The characterization results showed a polydispersity index of 0.14, an average diameter of 190.1± 3 nm, zeta potential of -38.5 mV and an entrapment efficiency of 73%. The release profile of Benzocaine loaded in PLA nanocapsules showed a significant different behavior than that of the pure anesthetic in solution. This study is important to characterize a drug release system using benzocaine for application in pain treatment.