980 resultados para Hormonal mutants
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Medicina Veterinária - FCAV
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Medicina Veterinária - FMVZ
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Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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As sementes de anonáceas são conhecidas por apresentarem mecanismos de dormência, o que dificulta a perpetuação das espécies e a formação de áreas produtivas para a exploração comercial. Deste modo, os objetivos deste trabalho foram estudar curva de aquisição de água; a germinação de sementes tratadas com GA3 e GA4+7 + Benziladenina; o balanço hormonal e a degradação de reservas em sementes de Annona diversifolia Saff e Annona purpurea Moc & Sessé ex Dunal tratadas com reguladores vegetais para a superação da dormência. Para tanto foram realizados três experimentos. Para a construção da curva de aquisição de água foram utilizadas 4 repetições de 25 sementes que foram mantidas em embebição e pesadas durante 480 horas. O segundo experimento foi constituído pela germinação das sementes tratadas com os reguladores vegetais; o delineamento experimental empregado foi o inteiramente casualizado com 4 repetições de 25 sementes por parcela em esquema fatorial 2 x 7 (reguladores x concentrações). Os tratamentos foram constituídos pelas combinações entre concentrações de GA3 e de GA4+7 + Benziladenina (GA4+7 + BA) x 0, 200, 400, 500, 600, 800 e 1000 mg L-1 i.a.. No terceiro experimento foram quantificados ABA (Ácido abscísico) e GA (Giberelinas), proteínas, açúcares...
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The aim of this study was to analyze photosynthate partitioning in tomato photomorphogenic mutants at the ends of the vegetative (40 days after emergence [DAE]) and reproductive (69 DAE) stages and to determine its interaction with morphoanatomical aspects. The mutants aurea (au), phytochrome-deficient, high pigment-1 (hp1), light-exaggerated response, were studied along with the non-mutant Micro-Tom (MT) cultivar. The plants were analyzed at 40 and 68 DAE to identify photosynthate source organs and tissues as well as the target organs of remobilized photosynthate during the reproductive stage. The plants were evaluated for their internal and external morphology as well as the percentage of dry mass of their organs. Photosynthate allocation in the hp1 mutant occurred primarily in the roots and leaves, and allocation in the au mutant occurred primarily in fruits. The au mutant showed a high capacity for photosynthate remobilization to fruit during the reproductive stage, and the predominant sources of these remobilized photosynthates were the leaf spongy parenchyma, the root vascular cylinder and the marrow stem.
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Pós-graduação em Biotecnologia Animal - FMVZ
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To assess the importance of the leucine residues in positions 262 and 265 of the angiotensin AT, receptor for signaling pathways and receptor expression and regulation, we compared the properties of CHO cells transfected with the wild type or the L262D or L265D receptor point mutants. It was found that the two mutants significantly increased the basal intracellular cyclic AMP (cAMP) formation in an agonist-independent mode. The morphology transformation of CHO cells was correlated with the increased cAMP formation, since forskolin, a direct activator of adenylate cyclase mimicked this effect on WT-expressing CHO cells. DNA synthesis was found to be inhibited in these cell lines, indicating that cAMP may also have determined the inhibitory effect on cell growth, in addition to the cell transformation from a tumorigenic to a non-tumorigenic phenotype. However a role for an increased Ca2(+) influx induced by the mutants in non-stimulated cells cannot be ruled out since this ion also was shown to cause transformed cells to regain the morphology and growth regulation. (c) 2005 Elsevier B.V. All rights reserved.
Effect of in vitro hormonal supplementation on the ultrastructure of oocyte from bitches at anestrus